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Ilmi Sufa, Hafizah
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Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology

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EFEKTIVITAS DEKONTAMINASI SPUTUM MENGGUNAKAN N-acetyl-L-cysteine–sodium citrate (NALC) - NaOH 4%, 5%, DAN 6% TERHADAP WAKTU PERTUMBUHAN Mycobacterium tuberculosis PADA BACTEC MGIT 960 Faisal, Ardian; Ilmi Sufa, Hafizah; Dermawan, Asep; Rahmat, Mamat; Maemunah, Iin
Jurnal Kesehatan Siliwangi Vol. 4 No. 3 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Tuberkulosis (TBC) merupakan penyakit menular yang disebabkan oleh bakteri Mycobacterium tuberculosis (MTB). Bakteri yang tahan asam ini, yang juga dikenal sebagai basil tahan asam, memiliki struktur berbentuk batang. Sebagaimana disebutkan World Health Organization (WHO), tuberkulosis menjadi masalah kesehatan di seluruh dunia. Akan ada 10,6 juta kasus tuberkulosis yang terdiagnosis di seluruh dunia pada tahun 2021, meningkat sekitar 600.000 kasus dari 10 juta kasus yang terdiagnosis pada tahun 2020. Saat ini Indonesia berada diposisi kedua di dunia setelah India. Teknik biakan dengan media cair yaitu MGIT memerlukan proses dekontaminasi sebagai salah satu proses yang menentukan tumbuhnya bakteri MTB dengan baik tanpa adanya kontaminasi bakteri lain, sehingga memudahkan pada pemeriksaan selanjutnya yaitu uji kepekaan. Tujuan penelitian adalah untuk menentukan Waktu dan Growth Unit MTB yang tumbuh dengan dekontaminasi pemberian NALC-NaOH 4%, 5% dan 6% pada waktu inkubasi 1,2, 3 dan 4 minggu. Metode penelitian yang dilakukan merupakan penelitian eksperimen. Penelitian ini dilaksanakan pada bulan Oktober-November 2023 didapatkan sampel sebanyak 10 pasien yang melakukan pengobatan TB Diagnosis dan Follow Up MDR di RS. Paru M. Goenawan Partowidigdo. Hasil penelitian diperoleh rata-rata waktu pertumbuhan dan growth unit pada proses biakan dengan dekontaminasi NaLC-NaOH 4% memiliki rata-rata waktu pertumbuhan 10 hari 12 jam dan growth unit pada alat 148,5, konsentrasi NaLc-NaOH 5% memiliki rata-rata waktu pertumbuhan 10 hari 18 jam dan growth unit pada alat 155,9 dan konsentrasi NaLc-NaOH 6% memiliki rata-rata waktu pertumbuhan 11 hari dan growth unit pada alat 143,2. Disimpulkan dekontaminasi sputum menggunakan NaLC-NaOH dengan berbagai konsentrasi efektivitas baik pada NaLC-NaOH 5%.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum L.) TERHADAP Streptococcus pyogenes Ghaisani, Syifa Marha; Kurniati, Iis; Ilmi Sufa, Hafizah; Rahmat, Mamat
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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One of the most common bacterial infections is pharyngitis. Most cases of acute pharyngitis are caused by viruses, but the main bacterial agent is Streptococcus pyogenes. Some antibiotics have been resistant to Streptococcus pyogenes, so alternative antibacterials are needed, namely the use of clove leaves (Syzygium aromaticum L.). The type of research used is Quasi Experiment. This study was conducted to test the antibacterial activity of clove leaves against Streptococcus pyogenes. Clove leaf extract was obtained by maceration method using 96% ethanol solvent. Phytochemical tests on clove leaf extracts showed positive leaves containing saponins, phenols, terpenoids, flavonoids, and tannins. Antibacterial activity test was conducted by disc diffusion method with variation of extract concentration of 10%, 15%, and 25% and incubation time for 24 and 48 hours. Bacitracin disc was used as positive control and negative control used 10% DMSO. The test results showed that there was antibacterial activity indicated by the formation of inhibition zone at 25% concentration with an average of 9.23 mm and 15% concentration of 8.8 mm. The zone of inhibition formed did not match the zone of inhibition in the control (≥13 mm).
POTENSI EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) DALAM MENGHAMBAT PERTUMBUHAN Streptococcus pyogenes Nurdini, Lia; Dermawan, Asep; Ilmi Sufa, Hafizah; Rohayati
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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S. pyogenes is the primary cause of acute pharyngitis. The inappropriate, repeated, and irregular use of antibiotics can lead to resistance. One alternative is to utilize plants containing antibacterial compounds. This study aims to identify the concentration and contact time of fragrant pandan leaf extract that has the potential to inhibit the growth of S. pyogenes. The method applied in this study is the disk diffusion test with fragrant pandan leaf extract at concentrations of 20%, 40%, and 60%, with contact periods of 24 hours and 48 hours. Bacitracin was used as a positive control, and 10% DMSO as a negative control. The data were analyzed using a Two Way ANOVA test, showing a significant difference between the extract concentration and contact time on the inhibition zone diameter (p<0.05). The study resulted in an average inhibition zone diameter at a 20% concentration of 9 mm after 24 hours, 40% at 9.9 mm, and 60% at 11 mm. For the 48-hour contact time, the average inhibition zone diameter at a 20% concentration was 8.9 mm, 40% was 9.7 mm, and 60% was 10.8 mm. The average inhibition zone diameter in the positive control (bacitracin) was 18.3 mm, while the negative control showed no inhibition zone.
OPTIMASI MEDIA ALTERNATIF NUTRIENT AGAR BERBAHAN KACANG MERAH (Phaseolus vulgaris L.) DALAM MENUMBUHKAN Staphylococcus aureus Mutiara Sulam, Nuri; Ilmi Sufa, Hafizah; Dermawan, Asep; Rismiati, Zuri
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Media are used to grow bacteria to diagnose infectious diseases, stock bacterial cultures, and in educational institutes for learning and research on the characteristic of bacteria. One of these media is nutrient agar which contains nutrients in the form of protein and carbohydrates. Growth media has less affordable prices so alternative media are created. Alternative media to replace nutrient agar with a vegetable protein source to replace the protein contained in the media. For example, the vegetable protein used is from red beans. Red beans contain protein and amino acids which will replace protein in nutrient agar media. This research aims to determine alternative media made from red beans that can be used as a substitute for nutrient agar, the optimum concentration and boiling time of red beans in growing Staphylococcus aureus with concentration variations of 5%, 10%, and 15% and time variations of 5 minutes, 10 minutes, and 15 minutes. In the research, inoculation was carried out using the pouring cup method. The results of the number of colonies on the alternative media will be compared with the results of the number of colonies on the control, namely nutrient agar. Based on the Generalized Linear Model (GLM) statistical test, an alternative media with a concentration of 10% and a boiling time of 10 minutes can be used as a substitute for nutrient agar.
Co-Authors Dermawan, Asep Faisal, Ardian Ghaisani, Syifa Marha Iis Kurniati Maemunah, Iin Mamat Rahmat Mutiara Sulam, Nuri Nurdini, Lia Rismiati, Zuri Rohayati