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All Journal Jurnal Riset Kesehatan Poltekkes Depkes Bandung JMLS: Journal of Medical Laboratory and Science Current Biomedicine Mukhtabar: Journal of Medical Laboratory Technology Jurnal Kesehatan Siliwangi
Dermawan, Asep
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Analysis of Influencing Factors on Participation Compliance, Slide Preparations Quality and Microscopic Reading Quality of Acid-Resistant Bacteria Slides at Ciamis Regency Sriwardani, I Dewa Ayu; Dermawan, Asep; Kurniati, Iis; Feisal, Sonny
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 1 (2024): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i1.544

Abstract

Background & Objective: To find out the factors that influence participation compliance, slide preparations quality and microscopic reading quality in the External Quality Control program of Microscopic Cross Tests Acid Resistant Bacteria in Ciamis Regency in 2022-2023 Method: The research design is cross-sectional, population and total sampling of 27 respondents. Data analysis is Chi-Square with Fisher's Exact Test Result: Research data shows that the sig-p value of the period of employment variable for participation is 0.057, for the quality of slide preparations per quarter 0.695, 0.447, 0.550, 0.305, and the microscopic reading quality 0.036. The sig-p value of the training variable on participation is 0.226, on the quality of slide preparations per quarter 0.043, 0.388, 0.212, 0.219, and the microscopic reading quality 0.298. The sig-p value of the workload variable for participation is 0.204, for the quality of slide preparations per quarter 0.502, 0.091, 0.332, 0.502, and for the microscopic reading quality 0.050. The sig-p value of the microscope condition variable for participation is 0.005, for the quality of slide preparations per quarter 0.063, 0.106, 0.149, 0.323, and for the microscopic reading quality 0.036. The sig-p value of the incentive variable for participation is 0.001, for the quality of slide preparations per quarter 0.528, 0.302, 0.586, 0.189, for the microscopic reading quality of 0.013 Conclusion: there is a relationship between microscope conditions and incentive on participation compliance, there is a relationship between training on the slide preparations quality in the 4th quarter of 2022, there is a relationship between period of employment, workload, microscope conditions and incentive on microscopic reading quality
EFEKTIVITAS DEKONTAMINASI SPUTUM MENGGUNAKAN N-acetyl-L-cysteine–sodium citrate (NALC) - NaOH 4%, 5%, DAN 6% TERHADAP WAKTU PERTUMBUHAN Mycobacterium tuberculosis PADA BACTEC MGIT 960 Faisal, Ardian; Ilmi Sufa, Hafizah; Dermawan, Asep; Rahmat, Mamat; Maemunah, Iin
Jurnal Kesehatan Siliwangi Vol. 4 No. 3 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Tuberkulosis (TBC) merupakan penyakit menular yang disebabkan oleh bakteri Mycobacterium tuberculosis (MTB). Bakteri yang tahan asam ini, yang juga dikenal sebagai basil tahan asam, memiliki struktur berbentuk batang. Sebagaimana disebutkan World Health Organization (WHO), tuberkulosis menjadi masalah kesehatan di seluruh dunia. Akan ada 10,6 juta kasus tuberkulosis yang terdiagnosis di seluruh dunia pada tahun 2021, meningkat sekitar 600.000 kasus dari 10 juta kasus yang terdiagnosis pada tahun 2020. Saat ini Indonesia berada diposisi kedua di dunia setelah India. Teknik biakan dengan media cair yaitu MGIT memerlukan proses dekontaminasi sebagai salah satu proses yang menentukan tumbuhnya bakteri MTB dengan baik tanpa adanya kontaminasi bakteri lain, sehingga memudahkan pada pemeriksaan selanjutnya yaitu uji kepekaan. Tujuan penelitian adalah untuk menentukan Waktu dan Growth Unit MTB yang tumbuh dengan dekontaminasi pemberian NALC-NaOH 4%, 5% dan 6% pada waktu inkubasi 1,2, 3 dan 4 minggu. Metode penelitian yang dilakukan merupakan penelitian eksperimen. Penelitian ini dilaksanakan pada bulan Oktober-November 2023 didapatkan sampel sebanyak 10 pasien yang melakukan pengobatan TB Diagnosis dan Follow Up MDR di RS. Paru M. Goenawan Partowidigdo. Hasil penelitian diperoleh rata-rata waktu pertumbuhan dan growth unit pada proses biakan dengan dekontaminasi NaLC-NaOH 4% memiliki rata-rata waktu pertumbuhan 10 hari 12 jam dan growth unit pada alat 148,5, konsentrasi NaLc-NaOH 5% memiliki rata-rata waktu pertumbuhan 10 hari 18 jam dan growth unit pada alat 155,9 dan konsentrasi NaLc-NaOH 6% memiliki rata-rata waktu pertumbuhan 11 hari dan growth unit pada alat 143,2. Disimpulkan dekontaminasi sputum menggunakan NaLC-NaOH dengan berbagai konsentrasi efektivitas baik pada NaLC-NaOH 5%.
PENGARUH DISINFEKTAN TERHADAP PERTUMBUHAN Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, dan Bacillus subtilis ATCC 6633 Ridwan, Farid Muchammad; Wahyuni, Yeni; Solihat, Mohamad Firman; Dermawan, Asep
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 2 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i2.2268

Abstract

Kontaminasi mikroorganisme baik bakteri, yeast, mould, ataupun virus merupakan masalah yang serius di laboratorium mikrobiologi maupun di fasilitas pelayanan kesehatan. Pengendalian terhadap bakteri kontaminan di laboratorium dapat dilakukan dengan pemantauan lingkungan atau Environmental monitoring (EM). Tujuan Environmental monitoring adalah untuk memastikan jumlah partikel di udara dalam batas yang ditentukan, memperkirakan keefektifan proses pembersihan dan proses disinfeksi. Penggunaan disinfektan secara benar dan metode penggunaan yang tepat merupakan elemen dasar dalam pencegahan infeksi pada layanan kesehatan. Efektivitas prosedur desinfeksi bergantung pada banyak faktor (misalnya, waktu kontak, konsentrasi akhir bahan aktif). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh disinfektan terhadap pertumbuhan Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, dan Bacillus subtilis ATCC 6633. Jenis Penelitian yang dilakukan adalah Quasi Eksperimen. Sampel Alkohol 70%, Isopropil Alkohol 70%, dan Benzalkonium klorida 0,75% dipaparkan terhadap S. aureus ATCC 6538, P. aeruginosa ATCC 9027, dan B. subtilis ATCC 6633 dengan Waktu kontak 1, 5, dan 10 menit. Analisis data secara statistik pada penelitian menggunakan uji Cochran Q. Metode penelitian menggunakan Hard Surface Carrier Test. Hasil dari analisis uji Cochran Q menunjukkan nilai Asymptotic Significance > 0.05. Berdasarkan hasil uji statistik tersebut dapat disimpulkan tidak terdapat perbedaan bermakna antara waktu kontak (1 menit, 5 menit dan 10 menit) dan disinfektan (Alkohol 70%, Isopropanol 70%, Benzalkonium klorida 0,75%) terhadap pertumbuhan Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, dan Bacillus subtilis ATCC 6633.
POLA KEPEKAAN BAKTERI RESISTEN KATEGORI RESERVE PADA SPESIMEN MIKROBIOLOGIS DI RUMAH SAKIT ADVENT BANDUNG Triani, Novita Ajeng; Dermawan, Asep
Journal of Medical Laboratory and Science Vol 4 No 2 (2024): JMLS: Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/medlabscience.v4i2.2104

Abstract

Background: Microbial resistance to antimicrobials is a global health problem. In 2017 WHO divided antibiotics into AWaRe categories: Access, Watch and Reserve. The reserve category is a reserve antibiotic, only available in advanced health care facilities, reviewed by pharmacists, and approved by the hospital's PGA and PPRA. Therefore, the aim of this study was to determine the sensitivity pattern of reserve category antibiotic-resistant bacteria in microbiological specimens in hospitals Bandung Adventist. Methods: This research is a descriptive study, namely collecting data on events that have already occurred, taken from the results of culture examinations and bacterial sensitivity tests at the Bandung Adventist Hospital for the period August 2022 - August 2023 as secondary data, and primary data from September - October 2023. Results: Results research from 996 specimens sent to the laboratory, obtained 217 bacterial isolates (21.79%). Based on Gram staining, Gram negative was known to have a total of 169 isolates (77.89%), while Gram positive contained 48 isolates (22.11%). The most common susceptibility pattern of resistant bacteria in the reserve category in Gram negative was Acinetobacter baumannii, from 34 isolates 28 isolates were found to be resistant (82.35%), and Salmonella typhi from 4 isolates were all resistant (100%) to the antibiotic Cefepime. In the antibiotic Aztreonam, only Pseudomonas aeruginosa were used. Of the 31 isolates, 13 isolates were found to be resistant (41.93%), while in the Colistin class of antibiotics the most resistance was found in Pseudomonas aeruginosa, of the 31 isolates, 4 isolates were found to be resistant (12.90%). ). Of the Gram positives examined only the antibiotic Linezolid was found mostly in Staphylococcus aureus, from 29 isolates 3 isolates were found to be resistant (10.34%). Keywords : AWaRe, Acces, Watch, Reserve
Confirmation of Mycobacterium tuberculosis culture results with Ziehl-Neelsen staining and MPT64 antigen test Indrasari, Witri; Kurniati, Iis; Dermawan, Asep; Sufa, Hafizah Ilmi
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.11

Abstract

Background Culture of Mycobacterium tuberculosis (MTB) using egg-based solid media like Lowenstein Jensen (LJ) is the gold standard for tuberculosis diagnosis but requires extended incubation time. Rapid diagnostic tests, such as Ziehl-Neelsen (ZN) staining and the MPT64 antigen rapid test, are essential for early detection. Objective This study aimed to evaluate the effectiveness of ZN staining and the MPT64 rapid test in detecting MTB and Mycobacterium other than tuberculosis (MOTT) during different culture times. Methods Using a cross-sectional design, 110 culture-positive samples were analyzed from Hasan Sadikin Hospital Bandung over two months. Specimens were cultured on LJ media for eight weeks, with weekly observation of colony growth. ZN staining and MPT64 tests were performed on growing colonies. Results Less than four weeks culture, 61 samples (55.5%) were culture-negative, 45 (40.9%) were positive for MOTT, and the remainder were contaminated. In more than four weeks culture, 48 samples (43.6%) were positive for MTB, 45 samples (40.9%) were positive for MOTT, and 13 (11.8%) were culture-negative, and the remaining were contaminated. ZN-positive and MPT64-negative results indicated MOTT in less than four weeks culture, while ZN-positive and MPT64-positive results indicated MTB in more than four weeks culture. Conclusion While ZN staining was positive for both MTB and MOTT colonies, the MPT64 rapid antigen test was specific for MTB, supporting its use in confirming MTB detection alongside culture methods.
PERBEDAAN SUHU DAN WAKTU PENYIMPANAN SPESIMEN SPUTUM TERHADAP HASIL PEMERIKSAAN MIKROSKOPIS BAKTERI TAHAN ASAM Alfi Agilia Saputri, Nianda; Ilmi Sufa , Hafizah; Kurniati, Iis; Dermawan, Asep
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Sputum specimens should ideally be examined immediately to ensure accurate BTA (Acid Resistant Bacteria) microscopic examination results. The limited number of ATLMs while the large amount of sputum causes delays in examination. The purpose of the study was to determine the differences in characteristics and the number of BTA based on the International Union Againtst Tuberculosis and Lung Disease (IUATLD) scale of sputum examined immediately with those stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours. The research design used was quasi-experimental. The results showed there were changes in the characteristics of sputum stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours against sputum examined immediately. Sputum examined immediately had a BTA count of 1+. Sputum stored at temperature (23-27°C) at storage times of 6.5 and 7.5 hours the number of BTA is 1+, while sputum stored at temperature (35-39°C) at storage times of 6.5 and 7.5 hours the number of BTA is 2+. So it can be concluded that there are changes in the characteristics of sputum stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours. There is no difference in the number of BTA scale (IUATLD) of sputum stored at temperature (23-27°C) at storage times of 6.5 and 7.5 hours, while sputum stored at temperature (35-39°C) at storage times of 6.5 and 7.5 hours there is a difference in the number of BTA scale (IUATLD) of sputum specimens examined immediately.
EFEKTIVITAS KONSENTRASI DAN WAKTU MASERASI EKSTRAK DAUN BELUNTAS (Pluchea indica L.) TERHADAP PERTUMBUHAN Streptococcus pyogenes Ismi Yulandari, Siti; Dermawan, Asep; Kurniati, Iis; Iin Nur Indra, Asep
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

One of the most common diseases in Indonesia, namely pharyngitis, is caused by Streptococcus pyogenes. Inappropriate treatment of pharyngitis with antibiotics can result in bacteria that are resistant to antibacterials. Therefore, herbal plants that are effective as natural antibacterials are needed, such as beluntas leaves (Pluchea indica L.) to prevent this. Apart from that, the right method is needed so that the contents of the beluntas leaves can be attracted properly. The aim of this research was to determine the effective concentration and maceration time of beluntas leaf extract in inhibiting the growth of S.pyogenes. This research used varying maceration times of 24 and 72 hours. Then, from each variation of maceration time, beluntas leaf extract was made in concentrations of 5%, 10%, 15%, 20%, and 25%.  Beluntas leaf extract was tested for its inhibitory power against S.pyogenes using the Kirby Bauer method. The data obtained was the diameter of the inhibitory power of beluntas leaf extract on the growth of S.pyogenes, then the data was processed statistically using the Kruskal-Wallis test and a further test, namely the Post Hoc Test. The results of this research were that beluntas leaf extract which was macerated for 72 hours with a concentration of 20% and 25% had an average diameter of inhibition against S.pyogenes of 9.04 mm and 12.71 mm. Therefore, a maceration time of 72 hours with a concentration of 25% is effective in inhibiting the growth of S.pyogenes.
OPTIMASI WAKTU INKUBASI VAKSIN H5N1 PADA TELUR AYAM BEREMBRIO DENGAN SUHU 37OC TERHADAP TITER HEMAGLUTINASI Fuji Lestary, Rizka; Kurniati, Iis; Dermawan, Asep; Riyani, Ani
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Avian Influenza virus subtype H5N1 can infect animals as well as humans due to its high pathogenicity. Avian Influenza virus contains hemagglutinin protein that can agglutinate red blood cells. The purpose of this study was to determine the optimal incubation time, namely 3 days, 4 days, and 5 days and whether there are differences in the different incubation times. The type of research used was descriptive. The samples used were Avian Influenza Vaccine subtype H5N1 inoculated into embryonated chicken eggs aged 9-11 days. The allantois fluid produced from vaccine inoculation in embryonated chicken eggs can be tested for hemagglutination to determine its titer. The results showed that the optimum incubation time was 3 days and the optimum hemagglutination titer at the 3rd day incubation time was 1/256, at the 4th day incubation time was 1/256, and at the 5th day incubation time was 1/512. The data were analyzed using One-Way Anova test on SPSS. In conclusion, there was a significant difference in hemagglutination titer in the H5N1 vaccine inoculated in embryonated chicken eggs for 3 days and 4 days against the incubation time for 5 days.
POTENSI EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) DALAM MENGHAMBAT PERTUMBUHAN Streptococcus pyogenes Nurdini, Lia; Dermawan, Asep; Ilmi Sufa, Hafizah; Rohayati
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

S. pyogenes is the primary cause of acute pharyngitis. The inappropriate, repeated, and irregular use of antibiotics can lead to resistance. One alternative is to utilize plants containing antibacterial compounds. This study aims to identify the concentration and contact time of fragrant pandan leaf extract that has the potential to inhibit the growth of S. pyogenes. The method applied in this study is the disk diffusion test with fragrant pandan leaf extract at concentrations of 20%, 40%, and 60%, with contact periods of 24 hours and 48 hours. Bacitracin was used as a positive control, and 10% DMSO as a negative control. The data were analyzed using a Two Way ANOVA test, showing a significant difference between the extract concentration and contact time on the inhibition zone diameter (p<0.05). The study resulted in an average inhibition zone diameter at a 20% concentration of 9 mm after 24 hours, 40% at 9.9 mm, and 60% at 11 mm. For the 48-hour contact time, the average inhibition zone diameter at a 20% concentration was 8.9 mm, 40% was 9.7 mm, and 60% was 10.8 mm. The average inhibition zone diameter in the positive control (bacitracin) was 18.3 mm, while the negative control showed no inhibition zone.
OPTIMASI MEDIA ALTERNATIF NUTRIENT AGAR BERBAHAN KACANG MERAH (Phaseolus vulgaris L.) DALAM MENUMBUHKAN Staphylococcus aureus Mutiara Sulam, Nuri; Ilmi Sufa, Hafizah; Dermawan, Asep; Rismiati, Zuri
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Media are used to grow bacteria to diagnose infectious diseases, stock bacterial cultures, and in educational institutes for learning and research on the characteristic of bacteria. One of these media is nutrient agar which contains nutrients in the form of protein and carbohydrates. Growth media has less affordable prices so alternative media are created. Alternative media to replace nutrient agar with a vegetable protein source to replace the protein contained in the media. For example, the vegetable protein used is from red beans. Red beans contain protein and amino acids which will replace protein in nutrient agar media. This research aims to determine alternative media made from red beans that can be used as a substitute for nutrient agar, the optimum concentration and boiling time of red beans in growing Staphylococcus aureus with concentration variations of 5%, 10%, and 15% and time variations of 5 minutes, 10 minutes, and 15 minutes. In the research, inoculation was carried out using the pouring cup method. The results of the number of colonies on the alternative media will be compared with the results of the number of colonies on the control, namely nutrient agar. Based on the Generalized Linear Model (GLM) statistical test, an alternative media with a concentration of 10% and a boiling time of 10 minutes can be used as a substitute for nutrient agar.
Co-Authors Abror, Yogi Khoirul Alfi Agilia Saputri, Nianda Faisal, Ardian Feisal, Sonny Fuji Lestary, Rizka Iin Nur Indra, Asep Iis Kurniati Ilmi Sufa , Hafizah Ilmi Sufa, Hafizah Indrasari, Witri Ismi Yulandari, Siti Maemunah, Iin Mamat Rahmat Maryani, Nita Mutiara Sulam, Nuri Nurdini, Lia Nurhayati, Betty Ridwan, Farid Muchammad Rismiati, Zuri Riyani, Ani Rohayati Solihat, Mohamad Firman Sriwardani, I Dewa Ayu Sufa, Hafizah Ilmi Thayyiba, Amina Triani, Novita Ajeng Yeni Wahyuni