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All Journal JMLS: Journal of Medical Laboratory and Science Current Biomedicine Mukhtabar: Journal of Medical Laboratory Technology Jurnal Kesehatan Siliwangi Jurnal Laboratorium Khatulistiwa Jurnal Pengabdian Masyarakat Kesehatan Indonesia
Iis Kurniati
Politeknik Kesehatan Jurusan Analis Kesehatan, Departemen Kesehatan Provinsi Jawa Barat, Babakan Loa Cimahi Utara
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Analysis of Influencing Factors on Participation Compliance, Slide Preparations Quality and Microscopic Reading Quality of Acid-Resistant Bacteria Slides at Ciamis Regency Sriwardani, I Dewa Ayu; Dermawan, Asep; Kurniati, Iis; Feisal, Sonny
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 1 (2024): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i1.544

Abstract

Background & Objective: To find out the factors that influence participation compliance, slide preparations quality and microscopic reading quality in the External Quality Control program of Microscopic Cross Tests Acid Resistant Bacteria in Ciamis Regency in 2022-2023 Method: The research design is cross-sectional, population and total sampling of 27 respondents. Data analysis is Chi-Square with Fisher's Exact Test Result: Research data shows that the sig-p value of the period of employment variable for participation is 0.057, for the quality of slide preparations per quarter 0.695, 0.447, 0.550, 0.305, and the microscopic reading quality 0.036. The sig-p value of the training variable on participation is 0.226, on the quality of slide preparations per quarter 0.043, 0.388, 0.212, 0.219, and the microscopic reading quality 0.298. The sig-p value of the workload variable for participation is 0.204, for the quality of slide preparations per quarter 0.502, 0.091, 0.332, 0.502, and for the microscopic reading quality 0.050. The sig-p value of the microscope condition variable for participation is 0.005, for the quality of slide preparations per quarter 0.063, 0.106, 0.149, 0.323, and for the microscopic reading quality 0.036. The sig-p value of the incentive variable for participation is 0.001, for the quality of slide preparations per quarter 0.528, 0.302, 0.586, 0.189, for the microscopic reading quality of 0.013 Conclusion: there is a relationship between microscope conditions and incentive on participation compliance, there is a relationship between training on the slide preparations quality in the 4th quarter of 2022, there is a relationship between period of employment, workload, microscope conditions and incentive on microscopic reading quality
ANALISIS KADAR ASAM ASETAT (CH3COOH) PADA BUAH PISANG AMBON YANG DIFERMENTASI SELAMA 7, 10, 14, DAN 21 HARI Triana, Linda; Ratnawati, Gervacia Jenny; Kurniati, Iis; Sari, Emilda
Jurnal Laboratorium Khatulistiwa Vol 5, No 2 (2022): Mei 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v5i2.973

Abstract

Banana trees are plants that come from the humid tropics and include herbaceous plants from Southeast Asia, including Indonesian. Several types of bananas are scattered in various parts of Indonesia, namely Ambon bananas, Ambon moss bananas, Barangan bananas, Emas bananas, Kepok bananas, Lampung bananas, jackfruit bananas, horn bananas, and Raja Bulu bananas.One of the food ingredients that can be processed into processed products that have commercial value is bananas. Processing of bananas consists of two processing groups, that is: processing of unripe bananas and processing of ripe (ripe) bananas. One of the processing of ripe bananas can be done by fermentation. The end products of fermentation include energy, carbon dioxide, ethanol, ammonia, lactic acid, and acetic acid. Normally, acetic acid can be consumed after ripe bananas have been fermented for at least 3 days. Making acetic acid with bananas as raw material is called banana vinegar. This study aimed to observe differences in the levels of acetic acid (CH3COOH) in Ambon bananas fermented for 7, 10, 14, and 21 days. The results of the study on average levels of acetic acid in Ambon bananas fermented for 7, 10, 14 and 21 days were 0.59%, 0.68%, 0.77% and 0.84%. And from the results of statistic (p = 0.062 at the 95% confidence level (p > 0.05)).
PREVALENSI PENDERITA TUBERKULOSIS DI POLI DIRECTLY OBSERVED TREATMENT SHORT-COURSE (DOTS) RSUD CIDERES Prananda, Crystian Dwi; Kurniati, Iis; Sufa, Hafizah Ilmi; Solihat, Mohamad Firman
Journal of Medical Laboratory and Science Vol 4 No 2 (2024): JMLS: Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/medlabscience.v4i2.2100

Abstract

Latar Belakang: Berdasarkan laporan kegiatan layanan TB Directly Observed Treatment, Short-course (DOTS) di Rumah Sakit Umum Daerah Cideres penemuan suspek penderita BTA tahun 2021 ada 339 kasus yang dicurigai menderita BTA, 32 di antaranya positif BTA. Tahun 2022, 160 kasus dicurigai menderita BTA, 100 di antaranya positif BTA. Tujuan penelitian ini adalah untuk menentukan prevalensi penderita Tuberkulosis paru BTA Positif di Rumah Sakit Umum Daerah Cideres pada bulan Agustus-Oktober 2023. Metode: Jenis penelitian ini adalah deskriptif korelatif. Sampel merupakan penderita Tuberkulosis paru BTA positif di RSUD Cideres Majalengka pada bulan Agustus – Oktober 2023. Hasil: Didapat bahwa pasien kejadian TB positif dengan persentase sebesar 70% ada pada pasien dengan karakteristik pendidikan SD - SMP, kurangnya pengetahuan tentang TB serta tidak memerlukan pencahayaan di dalam rumah. Kemudian untuk pasien yang memiliki hunian tak layak didapatkan kejadian TB positif sebesar 67%, pasien dengan rentang usia 41-70th keatas sebesar 63%. Kesimpulan: Berdasarkan usia, pasien TB positif di RSUD Cideres sebagian besar berusia 41 hingga 70 tahun, yang berjumlah 19 orang (63 %). Berdasarkan jenis kelamin, sebagian besar pasien TB positif laki-laki dan perempuan sama, dan 21 orang (70%) berdasarkan karakteristik pendidikan, dan 9 orang (30 %). Berdasarkan tingkat pendidikan, sebagian besar pasien TB positif berusia 41 hingga 70 tahun, yang berjumlah 19 orang (63 %). Kata kunci : Tuberkulosis, DOTS, BTA Positif
PERBEDAAN SUHU DAN WAKTU PENYIMPANAN SPESIMEN SPUTUM TERHADAP HASIL PEMERIKSAAN MIKROSKOPIS BAKTERI TAHAN ASAM Alfi Agilia Saputri, Nianda; Ilmi Sufa , Hafizah; Kurniati, Iis; Dermawan, Asep
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Sputum specimens should ideally be examined immediately to ensure accurate BTA (Acid Resistant Bacteria) microscopic examination results. The limited number of ATLMs while the large amount of sputum causes delays in examination. The purpose of the study was to determine the differences in characteristics and the number of BTA based on the International Union Againtst Tuberculosis and Lung Disease (IUATLD) scale of sputum examined immediately with those stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours. The research design used was quasi-experimental. The results showed there were changes in the characteristics of sputum stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours against sputum examined immediately. Sputum examined immediately had a BTA count of 1+. Sputum stored at temperature (23-27°C) at storage times of 6.5 and 7.5 hours the number of BTA is 1+, while sputum stored at temperature (35-39°C) at storage times of 6.5 and 7.5 hours the number of BTA is 2+. So it can be concluded that there are changes in the characteristics of sputum stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours. There is no difference in the number of BTA scale (IUATLD) of sputum stored at temperature (23-27°C) at storage times of 6.5 and 7.5 hours, while sputum stored at temperature (35-39°C) at storage times of 6.5 and 7.5 hours there is a difference in the number of BTA scale (IUATLD) of sputum specimens examined immediately.
EFEKTIVITAS KONSENTRASI DAN WAKTU MASERASI EKSTRAK DAUN BELUNTAS (Pluchea indica L.) TERHADAP PERTUMBUHAN Streptococcus pyogenes Ismi Yulandari, Siti; Dermawan, Asep; Kurniati, Iis; Iin Nur Indra, Asep
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

One of the most common diseases in Indonesia, namely pharyngitis, is caused by Streptococcus pyogenes. Inappropriate treatment of pharyngitis with antibiotics can result in bacteria that are resistant to antibacterials. Therefore, herbal plants that are effective as natural antibacterials are needed, such as beluntas leaves (Pluchea indica L.) to prevent this. Apart from that, the right method is needed so that the contents of the beluntas leaves can be attracted properly. The aim of this research was to determine the effective concentration and maceration time of beluntas leaf extract in inhibiting the growth of S.pyogenes. This research used varying maceration times of 24 and 72 hours. Then, from each variation of maceration time, beluntas leaf extract was made in concentrations of 5%, 10%, 15%, 20%, and 25%.  Beluntas leaf extract was tested for its inhibitory power against S.pyogenes using the Kirby Bauer method. The data obtained was the diameter of the inhibitory power of beluntas leaf extract on the growth of S.pyogenes, then the data was processed statistically using the Kruskal-Wallis test and a further test, namely the Post Hoc Test. The results of this research were that beluntas leaf extract which was macerated for 72 hours with a concentration of 20% and 25% had an average diameter of inhibition against S.pyogenes of 9.04 mm and 12.71 mm. Therefore, a maceration time of 72 hours with a concentration of 25% is effective in inhibiting the growth of S.pyogenes.
OPTIMASI WAKTU INKUBASI VAKSIN H5N1 PADA TELUR AYAM BEREMBRIO DENGAN SUHU 37OC TERHADAP TITER HEMAGLUTINASI Fuji Lestary, Rizka; Kurniati, Iis; Dermawan, Asep; Riyani, Ani
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Avian Influenza virus subtype H5N1 can infect animals as well as humans due to its high pathogenicity. Avian Influenza virus contains hemagglutinin protein that can agglutinate red blood cells. The purpose of this study was to determine the optimal incubation time, namely 3 days, 4 days, and 5 days and whether there are differences in the different incubation times. The type of research used was descriptive. The samples used were Avian Influenza Vaccine subtype H5N1 inoculated into embryonated chicken eggs aged 9-11 days. The allantois fluid produced from vaccine inoculation in embryonated chicken eggs can be tested for hemagglutination to determine its titer. The results showed that the optimum incubation time was 3 days and the optimum hemagglutination titer at the 3rd day incubation time was 1/256, at the 4th day incubation time was 1/256, and at the 5th day incubation time was 1/512. The data were analyzed using One-Way Anova test on SPSS. In conclusion, there was a significant difference in hemagglutination titer in the H5N1 vaccine inoculated in embryonated chicken eggs for 3 days and 4 days against the incubation time for 5 days.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum L.) TERHADAP Streptococcus pyogenes Ghaisani, Syifa Marha; Kurniati, Iis; Ilmi Sufa, Hafizah; Rahmat, Mamat
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

One of the most common bacterial infections is pharyngitis. Most cases of acute pharyngitis are caused by viruses, but the main bacterial agent is Streptococcus pyogenes. Some antibiotics have been resistant to Streptococcus pyogenes, so alternative antibacterials are needed, namely the use of clove leaves (Syzygium aromaticum L.). The type of research used is Quasi Experiment. This study was conducted to test the antibacterial activity of clove leaves against Streptococcus pyogenes. Clove leaf extract was obtained by maceration method using 96% ethanol solvent. Phytochemical tests on clove leaf extracts showed positive leaves containing saponins, phenols, terpenoids, flavonoids, and tannins. Antibacterial activity test was conducted by disc diffusion method with variation of extract concentration of 10%, 15%, and 25% and incubation time for 24 and 48 hours. Bacitracin disc was used as positive control and negative control used 10% DMSO. The test results showed that there was antibacterial activity indicated by the formation of inhibition zone at 25% concentration with an average of 9.23 mm and 15% concentration of 8.8 mm. The zone of inhibition formed did not match the zone of inhibition in the control (≥13 mm).
Optimasi Konsentrasi dan Waktu Kontak Ekstrak Daun Jati (Tectona grandis) sebagai Alternatif Safranin pada Pewarnaan Gram Syafitri, Andita Izmi; Sufa, Hafizah Ilmi; Kurniati, Iis; Merdekawati, Fusvita
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Safranin is a cationic dye and is one of the harmful substance, in addition to its relatively expensive price, safranin waste also has several harmful effects on health, so the various studies on the use of natural dyes as an alternative of safranin have been widely carried out. Teak leaves are one of the plants that have the potential to used as an alternative of safranin because they contain anthocyanin compounds. This study aims to determine the concentration and optimal contact time of teak leaf extract that can be used as an alternative of safranin. The research method used is a quasi-experiment by providing treatment with 25%, 50%, and 100% concentration variations also contact time variations of 0.5, 1, and 1.5 minutes. The results showed that teak leaf (Tectona grandis) extract could be used as an alternative of safranin in Gram staining of E. coli and S. aureus. The conclusion of this study is that the optimum concentration of teak leaf extract is 50%, and the optimum contact time of teak leaf extract in Gram staining is 0.5 minutes.
The COMPARISON OF THE QUALITY OF MALARIA PREPARATIONS FLOODED WITH SOAKED IN GIEMSA SOLUTION Yuliana, Elviani Mutiara Hoy; Mulia, Yuliansyah Sundara; Sulaeman, Sulaeman; Kurniati, Iis
Journal of Medical Laboratory and Science Vol 5 No 1 (2025): JMLS: Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/medlabscience.v5i1.2809

Abstract

: Malaria is an infectious disease caused of Plasmodium. Microscopic diagnosis by examining thick and thin blood smears stained with Giemsa is that still the "gold standard". Giemsa is generally given by immersing entire blood stock as in the procedure for making malaria preparations and Giemsa has given by soaking in Giemsa paint as in the procedure making cytology preparations. The aim of this study to determine the quality of malaria preparations stained using the technique of being soaked in Giemsa solution to compare of malaria preparations stained using the technique of being soaked in Giemsa solution. The type of research used was a quasi-experiment with a static group comparison design, 80 preparations were made of 40 blood samples containing Plasmodium which were stained using the flooding technique and soaked in Giemsa solution. From the research carried out, the results of staining using the immersion technique were 35 preparations of good quality and 5 preparations of poor quality and staining using the immersion technique contained 48 preparations of good quality and 2 preparations of poor quality. The results of statistical tests using the Mc Nemar test obtained a sig value. of 0.453 (> 0.05). The result of this research can be concluded that there is no significant difference between preparations stained using the flooding technique and immersion in Giemsa solution.
Effect of solvent variation on results of antibiotic susceptibility test using the disk diffusion method against Staphylococcus aureus Habibah, Firda Nurul; Sufa, Hafizah Ilmi; Kurniati, Iis; Rismiarti, Zuri
Current Biomedicine Vol. 3 No. 2 (2025): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.2.60

Abstract

Background Mueller-Hinton agar (MHA) is widely used for disk diffusion tests to assess antibiotic susceptibility in non-fastidious bacteria. The type of water used to prepare the MHA may have affected the test outcomes. Objective This study evaluated the effect of different water types as solvents on the antibiotic susceptibility test results of tetracycline and gentamicin against Staphylococcus aureus. Methods MHA was prepared using four types of water: distilled (control), bottled, reverse osmosis (RO), and municipal tap water (PDAM). Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method, and data were analyzed using One-Way ANOVA and Tukey’s Post Hoc test. Results The mean inhibition zones for tetracycline were 23.8 ± 0.99 mm (distilled), 23.4 ± 0.37 mm (bottled), 23.0 ± 0.43 mm (RO), and 17.8 ± 1.41 mm (tap), categorized as sensitive except for tap water (intermediate). For gentamicin, the zones were 11.7 ± 0.37 mm (distilled), 12.7 ± 0.77 mm (bottled), 9.8 ± 0.18 mm (RO), and 17.6 ± 1.19 mm (tap), with the first three classified as resistant and tap water as sensitive. The tap water results were significantly different (p<0.05) from those of the other antibiotics. Conclusion The use of non-standard solvents in MHA preparation, particularly tap water, may lead to inconsistent antibiotic susceptibility results. Standardized use of distilled water is recommended to ensure test accuracy and reliability.
Co-Authors Abror, Yogi Khoirul Alfi Agilia Saputri, Nianda Dermawan, Asep Feisal, Sonny Fuji Lestary, Rizka Ghaisani, Syifa Marha Habibah, Firda Nurul I Gede Sugiyanta Iin Nur Indra, Asep Ilmi Sufa , Hafizah Ilmi Sufa, Hafizah Indrasari, Witri Ismi Yulandari, Siti Linda Triana Mamat Rahmat Merdekawati, Fusvita Mulia, Yuliansyah Sundara Nina Marliana, Nina Patria, Cecep Prananda, Crystian Dwi Rahma Kurnia Sri Utami Ratnawaty, Gervacia Jenny Riyani, Ani Sari, Emilda Solihat, Mohamad Firman Sriwardani, I Dewa Ayu Sufa, Hafizah Ilmi Sulaeman Sulaeman Syafitri, Andita Izmi Yuliana, Elviani Mutiara Hoy Zuri Rismiarti