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Peptida Bioaktif: Menjelajahi Potensi Dan Tantangan Menuju Pangan Masa Depan Rusydan, Azka Muhammad; Zulfaidah, Nanda Tsalasani
Jurnal Farmasi SYIFA Vol 2 No 2 (2024): Jurnal Farmasi SYIFA
Publisher : CV. Wadah Publikasi Cendekia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63004/jfs.v2i2.461

Abstract

Peptida bioaktif merupakan fragmen protein spesifik yang tidak hanya memiliki fungsi sebagai nutrisi, namun juga memiliki efek positif pada fungsi, kondisi dan kesehatan tubuh. Peptida bioaktif dapat terbentuk selama proses proteolisis baik melalui hidrolisis enzimatik in vitro, fermentasi, maupun pemecahan selama pencernaan makanan di saluran cerna. Peptida bioaktif memiliki berbagai manfaat pada kesehatan manusia dengan mempengaruhi sistem pencernaan, endokrin, kardiovaskular, syaraf dan imunitas. Beberapa jenis peptida bioaktif juga telah diteliti dan diketahui memiliki aktivitas antihipertensi, antioksidan, imunomodulator, antimikroba, antihiperlipidemia, sitomodulator dan antigenotoksik. Peptida bioaktif juga merupakan komponen yang fleksibel dalam penggunaannya dikarenakan variasi rantai samping yang dapat berinteraksi dan bekerja pada berbagai jenis lingkungan. Beberapa tantangan dalam penggunaan bioaktif peptida adalah lingkungan asam pada lambung serta efek dari enzim protease pada lambung dan usus yang tentunya akan berdampak terhadap ketersediaan hayatinya. Selain itu, meskipun peptida dapat mencapai sirkulasi sistemik, peptida dapat mengalami inaktivasi yang menyebabkan peptida kehilangan aktivitasnya dan hanya berperan sebagai nutrisi. Peptida dengan berat molekul besar juga dapat mengalami kesulitan dalam proses penyerapannya di usus, menyebabkan hilangnya peptida yang bermanfaat dari sistem pencernaan. Meskipun begitu, peptida bioaktif memiliki potensi besar yang dapat dikembangkan. Review ini berfokus pada potensi peptida bioaktif sebagai pangan fungsional dan nutrasetikal.
The Effect of Variation in Solvent Concentration on Caffeine Content in Green Arabica Coffee Bean Extract (Coffea arabica) using UV-Vis Spectrophotometry Zulfaidah, Nanda Tsalasani; Kurniawati , Endah; Putri, Gita Herdittya; Widiani, Fitria
Journal of Food and Pharmaceutical Sciences Vol 12, No 3 (2024): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.17983

Abstract

Coffee is one type of beverage that is widely favoured by the community, Arabica coffee plants are a variant that grows a lot in Indonesia. Coffee beans contain various chemical compounds, one of which is caffeine. This study aims to determine the caffeine content with different solvent concentrations. Green Arabica coffee beans (Coffea arabica) were extracted using the maceration method. Ethanol extract 70% and ethanol extract 96% of green Arabica coffee beans were then fractionated in liquid-liquid extraction of chloroform and water phases. Then the analysis and determination of caffeine content were carried out with a UV-Vis Spectrophotometer. Qualitative tests were carried out using the Parry reagent color test showing positive results with a change in color to green. The results of the study in the form of a % caffeine content value to the weight of the extract sample, showed that in the 70% ethanol extract of green Arabica coffee beans contained a caffeine content of 10.115 ± 0.06% and the 96% ethanol extract of green Arabica coffee beans was 12.760 ± 0.580%. The results of the analysis showed that the amount of caffeine content had a significant difference with a p-value <0.05. So it can be concluded that the optimum solvent for extracting caffeine from green Arabica coffee bean extract is 96% solvent.
The Effect of Extraction Solvents towards Total Flavonoid Content and Total Phenolic Content of Butterfly Pea (Clitoria ternatea) Extract Kurniawati, Endah; Zulfaidah, Nanda Tsalasani; Amaliya, Siti Nur; Kurniawati, Siska Putri
Journal of Biotechnology and Natural Science Vol. 4 No. 2 (2024): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v4i2.12158

Abstract

Butterfly pea flower (Clitoria ternatea) has many benefits including antioxidant. The antioxidant activity was contributed by flavonoid and phenolic components. The choice of solvent is important to obtain the metabolite components that contribute to antioxidant activity. Therefore this study was conducted to find the ability of different solvent to obtain total flavonoid content (TFC) and total phenolic content (TPC) in C. ternatea. The fine powder of dried flower was extracted by different solvents (ethanol 70%, ethanol 96%, and methanol). Quercetin was used as standard in determination of TFC and gallic acid was used as standard in determination of TPC. The TFC value of ethanol 70%, ethanol 96% and methanol extract were 46,23±0,39; 28,19±0,03; and 16,87±0,11 mg QE/g extract respectively. The TPC value of ethanol 70%, ethanol 96% and methanol extract were 93,91±0,16; 78,63±0,16; 84,46±0,16 mg GAE/g extract respectively. Ethanol 70% extract had the highest yield value and the highest amount of TPC and TFC compare to other solvents. Thus, the amount of flavonoid and phenolic in ethanol 70% extract showed the promising potential benefit of C. ternatea as antioxidant.
The Effect of Variation in Solvent Concentration on Caffeine Content in Green Arabica Coffee Bean Extract (Coffea arabica) using UV-Vis Spectrophotometry Zulfaidah, Nanda Tsalasani; Kurniawati , Endah; Putri, Gita Herdittya; Widiani, Fitria
Journal of Food and Pharmaceutical Sciences Vol 12, No 3 (2024): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.17983

Abstract

Coffee is one type of beverage that is widely favoured by the community, Arabica coffee plants are a variant that grows a lot in Indonesia. Coffee beans contain various chemical compounds, one of which is caffeine. This study aims to determine the caffeine content with different solvent concentrations. Green Arabica coffee beans (Coffea arabica) were extracted using the maceration method. Ethanol extract 70% and ethanol extract 96% of green Arabica coffee beans were then fractionated in liquid-liquid extraction of chloroform and water phases. Then the analysis and determination of caffeine content were carried out with a UV-Vis Spectrophotometer. Qualitative tests were carried out using the Parry reagent color test showing positive results with a change in color to green. The results of the study in the form of a % caffeine content value to the weight of the extract sample, showed that in the 70% ethanol extract of green Arabica coffee beans contained a caffeine content of 10.115 ± 0.06% and the 96% ethanol extract of green Arabica coffee beans was 12.760 ± 0.580%. The results of the analysis showed that the amount of caffeine content had a significant difference with a p-value <0.05. So it can be concluded that the optimum solvent for extracting caffeine from green Arabica coffee bean extract is 96% solvent.
Formulasi dan Karakterisasi Fisik Masker Mata Hidrogel Berbasis Ekstrak Kulit Manggis (Garcinia mangostana L.) Larasati, Dwi; Zulfaidah, Nanda Tsalasani; Rif’at, Widhiana Putri; Daryanti, Edhita Putri
Majalah Farmaseutik Vol 21, No 3 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v21i3.101786

Abstract

Lingkaran hitam di bawah mata (LHBM) adalah kondisi kulit yang ditandai dengan kegelapan pada kelopak mata bawah akibat berbagai faktor, termasuk paparan sinar UV. Salah satu solusi untuk mengatasi masalah ini adalah dengan menggunakan masker mata hidrogel yang terbuat dari ekstrak kulit buah manggis (Garcinia mangostana L). Masker ini berfungsi sebagai penghantar antioksidan untuk kulit di bawah mata, karena kulit buah manggis kaya akan xanton yang memiliki sifat antioksidan. Penelitian ini bertujuan untuk menentukan konsentrasi ekstrak kulit buah manggis yang optimal untuk formulasi masker mata hidrogel dan mengevaluasi sifat fisiknya. Jenis penelitian ini adalah eksperimental, menggunakan ekstrak kulit buah manggis yang diperoleh melalui metode maserasi, diikuti dengan skrining fitokimia. Konsentrasi ekstrak yang diuji adalah F1 (0,5%), F2 (1%), dan F3 (2%). Pengujian terhadap sediaan masker mata hidrogel meliputi organoleptis, pengukuran bobot dan ketebalan masker, pH, daya mengembang, elastisitas, dan homogenitas. Hasil penelitian menunjukkan bahwa ekstrak kulit buah manggis mengandung alkaloid, flavonoid, saponin, triterpenoid, tanin, dan polifenol. Ketiga konsentrasi ekstrak (F1, F2, dan F3) dapat diformulasikan menjadi masker mata hidrogel. Masker dengan konsentrasi F2 (1%) menunjukkan sifat homogenitas, penampilan fisik, pH, daya mengembang, dan elastisitas yang baik, sehingga sebagai pilihan yang paling direkomendasikan untuk digunakan.
Biji Kurma sebagai Sumber Fitokimia dan Antioksidan untuk Minuman Fungsional Bebas Kafein Ningrum, Karina Primatyas; Zulfaidah, Nanda Tsalasani; Jannah, Nadia Miftahul
Jurnal Riset Farmasi Volume 5, No. 2, Desember 2025, Jurnal Riset Farmasi (JRF)
Publisher : UPT Publikasi Ilmiah Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jrf.v5i2.8567

Abstract

Abstract. Date seeds (Phoenix dactylifera L.) are abundant agro-industrial by-products that remain underutilized. Numerous studies have reported that date seeds contain phenolic and flavonoid compounds with notable antioxidant activity and are naturally caffeine-free, indicating their potential as functional beverage ingredients and coffee alternatives. However, systematic evidence summarizing the variability of bioactive composition, antioxidant capacity, and the influence of variety and processing methods remains limited and scattered across the literature. This study aimed to comprehensively evaluate the bioactive profile, antioxidant activity, and potential utilization of date seeds as a caffeine-free functional beverage through a systematic literature review. Literature searches were conducted following the PRISMA 2020 guidelines using PubMed, ScienceDirect, DOAJ, and Google Scholar, focusing on articles published between 2020 and 2025. The reviewed studies reported total phenolic contents ranging from 135 to 448 mg GAE and total flavonoid contents of 6.8–7.5 mg CE/g, with antioxidant activity classified as moderate to strong based on DPPH assays (IC₅₀: 28–49 µg/mL). Overall, the findings indicate that date seeds have potential for development as caffeine-free functional beverage ingredients; however, further standardized studies are required to support optimal product development. Abstrak. Biji kurma (Phoenix dactylifera L.) merupakan limbah agroindustri yang melimpah dan hingga kini belum dimanfaatkan secara optimal. Berbagai studi melaporkan bahwa biji kurma mengandung senyawa fenolik dan flavonoid dengan aktivitas antioksidan yang signifikan serta bersifat bebas kafein secara alami, sehingga berpotensi dikembangkan sebagai bahan minuman fungsional alternatif kopi. Namun, kajian ilmiah yang merangkum secara sistematis variasi kandungan bioaktif, aktivitas antioksidan, serta pengaruh varietas dan metode pengolahan biji kurma masih terbatas dan tersebar dalam berbagai publikasi.Penelitian ini bertujuan untuk mengkaji secara komprehensif profil senyawa bioaktif, aktivitas antioksidan, dan potensi pemanfaatan biji kurma sebagai minuman fungsional bebas kafein melalui pendekatan systematic literature review. Penelusuran literatur dilakukan mengikuti pedoman PRISMA 2020 pada basis data PubMed, ScienceDirect, DOAJ, dan Google Scholar untuk artikel terbitan tahun 2020–2025. Hasil kajian menunjukkan bahwa biji kurma memiliki kandungan total fenolik pada kisaran 135–448 mg GAE dan total flavonoid 6,8–7,5 mg CE/g, dengan aktivitas antioksidan kategori sedang hingga kuat berdasarkan uji DPPH (IC₅₀ 28–49 µg/mL). Secara keseluruhan, biji kurma berpotensi dikembangkan sebagai bahan baku minuman fungsional bebas kafein, meskipun penelitian lanjutan yang terstandarisasi masih diperlukan untuk mendukung pengembangan produk secara optimal.