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All Journal Research Journal of Life Science Jurnal Medik Veteriner
Akramsyah, Muhammad Ali
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Veterinary

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Risk Factors and Incidence of Escherichia coli Producing Extended-spectrum β-lactamase (ESBL) in Dairy Cattle Dameanti, Fidi Nur Aini Eka Puji; Akramsyah, Muhammad Ali; Hasan, Chyntia Silvi Yanti; Amanda, Jacky Teguh; Pratama, Alfaro Rikko; Fahmiantika, Reza; Tedja, Dhaneswara; Izofani, Safira; Sutrisno, Rahayu
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.213-218

Abstract

This study aimed to evaluate the risk factor and insidence of Extended-spectrum b-lactamase (ESBL) produced by Escherichia coli in dairy cattle. The samples obtained were 38 rectal swabs from dairy cows from the Successful Mutual Livestock Group, Deyeng Village, Kediri Regency, East Java. The study started with interviews and observations using a questionnaire to obtain data on risk factors (source of drinking water, cleanliness of feedlots, cleanliness of drinking places, administration of antibiotics, history of mastitis). A sampling of the rectal swab of cattle was carried out using Nutrient Broth as carrier media. Isolation of bacteria using Mac Conkey Agar (MCA) media with the antibiotic cefotaxime 1 mg/L. The addition of antibiotics is intended so that the bacteria that grow are coliform bacteria resistant to the cefotaxime antibiotic, a beta-lactam group. Bacterial isolation was continued with MCA media to enrich ESBL bacteria. Bacterial identification was continued on EMBA media, gram staining, IMViC biochemical test, TSIA, and urease to obtain isolates of E. coli bacteria. The E. coli isolates tested for ESBL Double Disc Synergy Test (DDST) phenotype. The close relationship between each risk factor and the incidence of ESBL-producing E. coli was tested statistically using the Spearman Rank correlation test. The results showed that the incidence of ESBL-producing E. coli in the tested samples was 21.05%, or there were eight positive samples of ESBL-producing E. coli. The risk factors for finding a positive incidence of E. coli producing ESBL in this study were 87% of the use of drinking water sources from wells, 25% did not pay attention to the cleanliness of drinking places, 25% did not pay attention to the cleanliness of eating places, 38% were given antibiotics during maintenance, and 100% had a history of mastitis. These risk factors were not statistically closely related to the incidence of ESBL-producing E. coli.
Incidence of Extended Spectrum Beta-Lactamase (ESBL) of Escherichia Coli Isolated from the Feces of Dairy Cattles in Blitar Dameanti, Fidi Nur Aini Eka Puji; Amri, Indah Amalia; Roosdiana, Anna; Pratama, Alfaro Rikko; Izofani, Safira; Fahmiantika, Reza; Tedja, Dhaneswara; Sutrisno, Rahayu; Akramsyah, Muhammad Ali
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.5

Abstract

Antibiotic resistance (AMR) has caused a serious global threat to human health, animal health and food security. The antibiotic resistance problem, especially in Gram-negative bacteria, is increasingly widespread in various areas and has increased mortality, morbidity, and incidence. This study aims to determine the incidence of ESBL E. coli and the AMR and MDR properties of E.coli isolated from the feces of dairy cattles in Blitar, which have not been reported. This research was conducted from June to September 2022 with 60 samples of dairy cattle feces in Blitar. Samples were isolated and identified until confirmed that 55 (91.6%) samples were E. coli. All samples showed AMR character with 100% streptomycin, 87.27% chloramphenicol, 85.45% ampicillin, 72.73% cefotaxime, 40% tetracycline, 21.82% ciprofloxacin, and 14.55% sulfamethoxazole-trimethoprim. Forty-nine samples were MDR, with 10.9% resistant in three groups, 34.5% in four groups, 29.1% in five groups, 10.9% in six groups, and 3.6% in seven groups. MDR E. coli isolates were then confirmed for ESBL using the DDST test. The incidence of ESBL-producing E. coli from dairy cattle feces in Blitar was 40% (22 isolates). The results of this study can be a warning in the human health, animal health and food security.
Co-Authors Amanda, Jacky Teguh Amri, Indah Amalia Anna Roosdiana Dameanti, Fidi Nur Aini Eka Puji Fahmiantika, Reza Hasan, Chyntia Silvi Yanti Izofani, Safira Pratama, Alfaro Rikko Sutrisno, Rahayu Tedja, Dhaneswara