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All Journal Buletin Udayana Mengabdi Research Journal of Life Science Jurnal Ternak Tropika Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal International Journal of Community Service Prosiding Seminar Nasional Unimus
Amri, Indah Amalia
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Socialization Of The Using Of Disinfectants In Cattle As An Effort To Prevent The Transmission Of Foot And Mouth Diseases (FMD) In Sumber Rejeki, Dau, Malang Tuska, Habib Syaiful Arif; Orlona, Vanessa Prudence; Amri, Indah Amalia; Aini, Fidi Nur; Residiwati, Gretania
International Journal Of Community Service Vol. 3 No. 4 (2023): November 2023 ( Indonesia - Republik Demokratik Timor Leste - Malaysia - USA -
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijcs.v3i4.217

Abstract

Foot and Mouth Disease (FMD) is a contagious animal disease that attacks livestock. The Sumber Rejeki Dairy Cattle Group in Gading Kulon Village, Malang Regency has 356 cows of the Friesian Holstein breed. Several cows in Sumber Rejeki has been infected with the PMK virus. This disease causes a decrease in milk production in infected cows, causing economic losses to KSP breeders. Source of fortune. One of the efforts to prevent its spread is to disinfect the cage. Disinfection of cages is effective enough to kill the FMD virus because the FMD virus does not have an outer membrane and is easily destroyed at pH <5 and >7. Community knowledge regarding disinfection is still low, so socialization is carried out regarding the provision of disinfectants in cages. After the socialization, there was an increase in the knowledge of the farmers by 41.5% through the pretest and posttest which were given before and after the socialization.
Identification of Colibacillosis and Antibiotic Resistance Test in Free-range Chicken: Identifikasi Kolibasilosis dan Uji Resistensi Antibiotik pada Ayam Kampung Wulandari, Eka; Amri, Indah Amalia
Veterinary Biomedical and Clinical Journal Vol. 4 No. 1 (2022)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2022.004.01.4

Abstract

Escherichia coli are normal flora in the digestive tract of animals and humans but can be opportunistic under certain conditions. Apart from normal flora, there are also pathogenic strains of the E. coli bacteria that can cause disease in the hospes. The disease that can be caused by E. coli bacteria is colibacillosis which often occurs in poultry. One of the clinical symptoms that appear in chickens suffering from colibasillosis is diarrhea which can arise due to enteritis. This study aims to identify the incidence of colibacillosis and antibiotic resistance of E. coli bacteria in Free-range Chicken. Clinical symptoms showed that the animal looked weak, had diarrhea, and had feces around the cloaca. The necropsy results showed the presence of hemorrhage in the duodenum and caecum. Samples of organ swabs that experienced hemorrhage were then tested for isolation and identification of E.coli bacteria and antibiotic resistance using the Kirby-Bauer method for five types of antibiotics, penicillin, bacitracin, gentamicin, cefadroxil, and ciprofloxacin. The test results showed that the Free-range Chicken had colibacillosis. The E. coli bacteria that cause colibacillosis were resistant to penicillin and bacitracin antibiotics, intermediates to cefadroxil, and sensitive to ciprofloxacin and gentamicin.
Effect of Oregano (Origanum vulgare) Extract on Kidney Histomorphometry and Histopathology in Mice (Mus musculus) Colibacillosis Model: Pengaruh Pemberian Ekstrak Oregano (Origanum vulgare) terhadap Histomorfometri dan Histopatologi Ginjal pada Mencit (Mus musculus) Model Kolibasilosis Alviano, Bian Febry Rohmana; Amri, Indah Amalia
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.4

Abstract

Colibacillosis is a disease that infects the digestive tract due to infection with the pathogen Escherichia coli. E. coli is able to cause bacteremia so that E. coli can reach the kidney through blood flowing to the kidney. To reduce the side effects of treatment using antibiotics, the herbal medicine oregano (Origanum vulgare) which contains phenols (carvacrol and thymol) works as an antibacterial. The benefits of this research are as a source of information and the basis for further research related to the use of oregano extract in the treatment of colibacillosis due to E. coli infection. The study used 20 male mice, divided into 5 applicable groups, namely K-, healthy mice were not given any treatment; K+ was induced by Streptomycin 20 mg and infected with E.coli 3 x 109 CFU/ml, 0.5 ml; P1 was induced by Streptomycin 20 mg and infected with E.coli 3 x 109 CFU/ml, 0.5 ml + Origanum vulgare extract 25 mg/kg; P2 was induced by Streptomycin 20 mg and infected with Escherichia coli 3 x 109 CFU/ml, 0.5ml + Origanum vulgare extract 50mg/kg; P3 was induced by Streptomycin 20 mg and infected with E.coli 3 x 109 CFU/ml, 0.5 ml + Origanum vulgare extract 100 mg/kg. Kidney was taken for histopathological preparations with hematoxylin eosin (HE) staining. After that, it was analyzed using Kruskal Wallis and continued with Mann Whitney. Histomorphometry semiquantitative measures the width of the Bowman's glomerular capsule and the length of the tubular lumen and then analyzed using One Way Analysis of Variance (ANOVA). The results of this study showed that oregano extract had an effect on histomorphometry and histopathology of renal, with an optimal dose is 5 mg/mice.
Toksisitas Larutan Perak Nitrat (AgNO3) pada Mencit Balb-c Berdasarkan Kadar SGPT dan SGOT Amri, Indah Amalia; Hendrasmara, Muhamad Ferian; Qosimah, Dahliatul; Aeka, Ajeng; Rickyawan, Nofan; Purwatiningsih, Wawid; Dameanti, Fidi Nur Aini Eka Puji
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.251-257

Abstract

Perak Nitrat (AgNO3) sering dimanfaatkan di bidang kesehatan sebagai bahan antimikroba. Efek pada organisme yang terpapar perak nitrat dapat mengakibatkan adanya akumulasi toksik pada hepar. Penelitian ini bertujuan mengetahui dampak toksisitas perak nitrat terhadap kadar SGPT dan SGOT pada mencit Balb-c. Hewan coba yang digunakan pada penelitian ini berjumlah 20 ekor mencit Balb-c dengan berat badan 25-30  gram dan umur 2-3 bulan serta masa pemberian perlakuan selama 14 hari. Perlakuan yang diberikan terdiri dari kelompok K- (tanpa induksi), P1 (100 µg/ml), P2 (200 µg/ml), P3 (300 µg/ml), P4 (400 µg/ml). Kadar SGPT dan SGOT diamati dengan menggunakan metode spektrofotometri. Data dianalisa menggunakan uji One Way ANOVA dengan tingkat kepercayaan 95%. Hasil dan kesimpulan menunjukkan induksi larutan perak nitrat pada mencit Balb-c dengan dosis P4 (400 µg/ml) dan volume induksi 0.5 ml dapat menyebabkan toksisitas pada mencit yang ditandai dengan peningkatan kadar SGPT dan SGOT.
Histopathological and Gastric Histomorphometry Analysis in Mice Colibacillosis Model Induced by Ethanol 96% Oregano Vulgare Extract Agustin, Yuliana; Amri, Indah Amalia; Dameanti, Fidi Nur Aini Eka Puji; Wisesa, Ida Bagus Gde Rama
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.162-169

Abstract

Colibacillosis is a disease caused by Escherichia coli which is a normal flora bacterium of the digestive system in humans and animals. Oregano contains carvacrol and thymol which provide an antibacterial. This study was used 20 male Balb/C mice age 2-3 weeks with an average weight of 20-25 grams and were divided into 5 groups with 4 replications: negative control (KN), positive control (KP) given by streptomycin dose of 20 mg/head. E. coli dose of 3 x 109 CFU, (P1) was given by streptomycin dose of 20 mg/head, E. coli dose of 3 x 109 CFU, then given oreganoextract dose of 5 mg/head, (P2) was given by streptomycin dose of 20 mg/head, E. coli dose of 3 x 109 CFU, then given oregano extract dose of 10 mg/head, (P3) was given by streptomycin dose of 20 mg/head, E. coli dose of 3 x 109 CFU, then oregano extract was given dose of 20 mg/head. The data were analyzed by descriptive comparative. The histopathology showed epithelial cell damage or erosion caused by inflammatory cells in KP, P1, P2, and P3. In study group given oregano extract dose of 20 mg/head, P3, inflammatory cells and epithelial erosion were lower. Based on the ANOVA test of day 3 and 6 after necropsy, P1, P2, and P3 show insignificant in villi length and width of the vili (p>0,05) compared to KP. The conclusion the result of the histopathology result is epithelial erosion in group with dose of 20mg/head oregano extract was able to relieve the symptoms of colibacillosis. The conclusion of histomorphometry of the length and width of the villi at P1, P2, P3 (p>0.05) was not significantly different from the positive control.
Efficacy of Oregano Extract Ointment on Fibroblast Cells and Epidermis in Albino Rats with Excisional Wound Model Lesmana, Muhammad Arfan; Amri, Indah Amalia; Cahyanissa, Ardilla Rachma; Geoputri, Kartika Myrnandari; Anisa, Agri Kaltaria; Hardian, Andreas Bandang
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.262-270

Abstract

This study aimed to determine the effect of oregano extract ointment therapy on the number of fibroblast cells and the epidermal thickness in excised wounds. The experimental animals used were 20 male Wistar strain albino rats, 12 weeks old, weighing 100–150 g, divided into 5 treatment groups i.e., (C-) not excised and without ointment, (C+) excised and without ointment, and the treatment group were excised using a 5 x 5 mm biopsy punch and treated with oregano extract ointment using concentrations of (T1) 3%, (T2) 6% and (T3) 9% twice daily for 14 days in an excised wound. The variables observed in this study were the number of fibroblast cells and the epidermal thickness by the HE staining method then measured using ImageJ. Data analysis used the One-Way ANOVA test followed by the Tukey test (p < 0,05). The results showed a decrease in the number of fibroblast cells and an increase in the epidermal thickness in the 6% concentration ointment. This study concluded that the administration of oregano extract ointment with a concentration of 6% was the optimal concentration in accelerating the final proliferative phase, characterized by a decrease in fibroblast cells and an increase in epidermal thickness.
Incidence of Extended Spectrum Beta-Lactamase (ESBL) of Escherichia Coli Isolated from the Feces of Dairy Cattles in Blitar Dameanti, Fidi Nur Aini Eka Puji; Amri, Indah Amalia; Roosdiana, Anna; Pratama, Alfaro Rikko; Izofani, Safira; Fahmiantika, Reza; Tedja, Dhaneswara; Sutrisno, Rahayu; Akramsyah, Muhammad Ali
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.5

Abstract

Antibiotic resistance (AMR) has caused a serious global threat to human health, animal health and food security. The antibiotic resistance problem, especially in Gram-negative bacteria, is increasingly widespread in various areas and has increased mortality, morbidity, and incidence. This study aims to determine the incidence of ESBL E. coli and the AMR and MDR properties of E.coli isolated from the feces of dairy cattles in Blitar, which have not been reported. This research was conducted from June to September 2022 with 60 samples of dairy cattle feces in Blitar. Samples were isolated and identified until confirmed that 55 (91.6%) samples were E. coli. All samples showed AMR character with 100% streptomycin, 87.27% chloramphenicol, 85.45% ampicillin, 72.73% cefotaxime, 40% tetracycline, 21.82% ciprofloxacin, and 14.55% sulfamethoxazole-trimethoprim. Forty-nine samples were MDR, with 10.9% resistant in three groups, 34.5% in four groups, 29.1% in five groups, 10.9% in six groups, and 3.6% in seven groups. MDR E. coli isolates were then confirmed for ESBL using the DDST test. The incidence of ESBL-producing E. coli from dairy cattle feces in Blitar was 40% (22 isolates). The results of this study can be a warning in the human health, animal health and food security.
PENINGKATAN SOFTSKILL DAN HARDSKILL BIOLOGI BAGI SISWA SMA MELALUI BASIC MICROBIOLOGY TRAINING Amri, Indah Amalia
Buletin Udayana Mengabdi Vol 23 No 5 (2024): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Mata pelajaran Biologi yang diajarkan pada Sekolah Menengah Atas (SMA) pada umumnya terbatas capaian membaca, menghafal dengan disertai pelaksanaaan praktikum, namun pelaksanaan praktikum ditingkat SMA cukup minim. Hal ini yang menjadi latar belakang pelaksanaan pengabdian masyarakat dengan judul Pengayaan Mata Pembelajaran Biologi ; Organisme Prokariotik dan Virus Pada Siswa SMA kelas X di Kota Malang Raya. Kegiatan ini bertujuan untuk mengajarkan siswa sekolah menengah teknik hands on secara langsung dengan konsep dasar yang telah diajarkan. Topik yang diajarkan adalah organisme prokariotik, virus, bakteri dan jamur. Kegiatan ini dilakukan secara luring dan daring, dimana pemberian materi dilakukan secara daring dan peningkatan kemampuan laboratorium dilakukan secara luring melalui praktikum pewarnaan bakteri dan jamur serta pengamatan secara mikros dan makros di Laboratorium Mikrobiologi dan Imunologi Fakultas Kedokteran Hewan Universitas Brawijaya. Kesimpulan dari kegiatan ini adalah terdapat peningkatan pengetahuan siswa berdasarkan peningkatan nilai, pretest sebelum melakukan hands on dengan rata-rata nilai 71,67, kemudian dilakukan posttest setelah melakukan hands on dengan rata-rata nilai 80,4 sehingga terdapat peningkatan nilai sebesar 12% (8,73) dan rekapitulasi IKM menunjukkan sebanyak 78% peserta menyatakan sangat puas dan 22% menyatakan puas.
Komunikasi, Informasi, dan Edukasi (KIE) Terhadap Penerapan Sanitasi Higiene dalam Upaya Pencegahan Infeksi LSD di Kud Kertajaya Kabupaten Kediri Auranzha, Avriell Jasmine; Kurniawati, Siti; Amri, Indah Amalia
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Lumpy Skin Disease (LSD) merupakan penyakit hewan menular strategis dengan terdapat nodul pada kulityang jelas batasnya, menonjol, dan berdiameter antara 2-5 cm yang dapat menyebabkan dampak negatifpada kesehatan ternak sapi perah dan ekonomi peternak. LSD disebabkan oleh virus Lumpy Skin DiseaseVirus (LSDV) dari genus Capripoxvirus. Virus LSD hanya menyerang pada sapi dan kerbau. Penularanutama LSD adalah melalui vektor dan kontak langsung antar ternak. Penyakit ini menyebabkan penurunanproduksi susu, infertilitas, dan abortus. Virus LSD dapat menyebabkan kerugian baik dari segi kesehatanternak maupun dari segi ekonomi dan sosial peternak. Salah satu daerah yang memiliki populasi ternakyang relatif besar adalah KUD Kertajaya, Desa Medowo. Peternak KUD Kertajaya Desa Medowomayoritas merupakan peternak tradisional yang mendapatkan informasi terkait LSD sehingga diperlukanadanya KIE dengan tujuan untuk meningkatkan pengetahuan peternak terkaitpentingnya sanitasi higienedalam upaya pencegahan infeksi LSD. Metode KIE yang digunakan adalah menggunakan mediainformasi(pamflet), presentasi, dan diskusi secara langsung dan penilaian terukur dari hasil pretest, posttest,dan SKM yang dianalisis secara deskriptif dan kuantitatif. Hasil pretest yang diperoleh menunjukkansebanyak 16,5% peternak masih belum memiliki pemahaman yang komprehensif mengenai pentingnyasanitasi higiene dalam upaya pencegahan infeksi LSD.  Hasil posttest diketahui adanya peningkatansebanyak 12,5% setelah dilakukan KIE. Data SKM menunjukkan adanya respon positif sebesar 88%.Kegiatan KIE ini dapat menginisiasi peternak untuk dapat meningkatkan pemahaman terkait LSD padahewan ternak di KUD Kertajaya, Desa Medowo. Kata Kunci: Lumpy Skin Disease, Sanitasi higiene, Komunikasi Informasi EdukasiAbstract
Antibiotic Sensitivity Profile of Pasteurella multocida in Cattle in Malang, Indonesia Dodik Prasetyo; Amri, Indah Amalia; Dian Siswanto; Rahayu Shafa Camila Sanjoyo; Monica Widyaningrum; Tanti Widya; Fidi Nur Eka Puji Dameanti
Veterinary Biomedical and Clinical Journal Vol. 6 No. 2 (2024):
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.02.4

Abstract

This study was conducted in Malang, Indonesia, to assess the prevalence and antibiotic susceptibility of Pasteurella multocida in cattle exhibiting clinical signs of respiratory disease. Nasal and tracheal swabs were collected from 50 cattle across multiple farms and tested for P. multocida. The isolates were identified through colony morphology, Gram staining, and biochemical tests, with 4 out of 50 samples (8%) testing positive for P. multocida. Antibiotic susceptibility was determined using the disk diffusion method, involving seven antibiotics commonly used in veterinary practice. Results showed that 75% of the isolates were sensitive to ceftriaxone, while amoxicillin-clavulanic acid was effective in 50% of cases. However, all isolates demonstrated resistance to erythromycin and tetracycline. These findings emphasize the importance of susceptibility testing to guide appropriate antibiotic therapy and prevent the development of resistance. Further research is necessary to monitor resistance patterns and improve treatment strategies for pasteurellosis in cattle.
Co-Authors Aeka, Ajeng Agustin, Yuliana Akramsyah, Muhammad Ali Albiruni Haryo, Albiruni Alviano, Bian Febry Rohmana Anisa, Agri Kaltaria Anna Roosdiana Auranzha, Avriell Jasmine Cahyanissa, Ardilla Rachma Dameanti, Fidi Nur Aini Eka Puji Dian Siswanto Dodik Prasetyo Eka Wulandari Fahmiantika, Reza Fajar Shodiq Permata Fidi Nur Eka Puji Dameanti Geoputri, Kartika Myrnandari Hardian, Andreas Bandang Hendrasmara, Muhamad Ferian Izofani, Safira Kurniawan, Septian Bestart Lesmana, Muhammad Arfan Monica Widyaningrum Noviatri, Aldila Orlona, Vanessa Prudence Paramanandi, Dini Agusti Pratama, Alfaro Rikko Purwatiningsih, Wawid Qosimah, Dahliatul Rahayu Shafa Camila Sanjoyo Residiwati, Gretania Rickyawan, Nofan Siti Kurniawati Sutrisno, Rahayu Tanti Widya Tedja, Dhaneswara Tuska, Habib Syaiful Arif Wisesa, Ida Bagus Gde Rama