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All Journal Research Journal of Life Science Jurnal Ternak Tropika Jurnal Medik Veteriner Journal of Applied Veterinary Science and Technology
Sutrisno, Rahayu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Veterinary

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Risk Factors and Incidence of Escherichia coli Producing Extended-spectrum β-lactamase (ESBL) in Dairy Cattle Dameanti, Fidi Nur Aini Eka Puji; Akramsyah, Muhammad Ali; Hasan, Chyntia Silvi Yanti; Amanda, Jacky Teguh; Pratama, Alfaro Rikko; Fahmiantika, Reza; Tedja, Dhaneswara; Izofani, Safira; Sutrisno, Rahayu
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.213-218

Abstract

This study aimed to evaluate the risk factor and insidence of Extended-spectrum b-lactamase (ESBL) produced by Escherichia coli in dairy cattle. The samples obtained were 38 rectal swabs from dairy cows from the Successful Mutual Livestock Group, Deyeng Village, Kediri Regency, East Java. The study started with interviews and observations using a questionnaire to obtain data on risk factors (source of drinking water, cleanliness of feedlots, cleanliness of drinking places, administration of antibiotics, history of mastitis). A sampling of the rectal swab of cattle was carried out using Nutrient Broth as carrier media. Isolation of bacteria using Mac Conkey Agar (MCA) media with the antibiotic cefotaxime 1 mg/L. The addition of antibiotics is intended so that the bacteria that grow are coliform bacteria resistant to the cefotaxime antibiotic, a beta-lactam group. Bacterial isolation was continued with MCA media to enrich ESBL bacteria. Bacterial identification was continued on EMBA media, gram staining, IMViC biochemical test, TSIA, and urease to obtain isolates of E. coli bacteria. The E. coli isolates tested for ESBL Double Disc Synergy Test (DDST) phenotype. The close relationship between each risk factor and the incidence of ESBL-producing E. coli was tested statistically using the Spearman Rank correlation test. The results showed that the incidence of ESBL-producing E. coli in the tested samples was 21.05%, or there were eight positive samples of ESBL-producing E. coli. The risk factors for finding a positive incidence of E. coli producing ESBL in this study were 87% of the use of drinking water sources from wells, 25% did not pay attention to the cleanliness of drinking places, 25% did not pay attention to the cleanliness of eating places, 38% were given antibiotics during maintenance, and 100% had a history of mastitis. These risk factors were not statistically closely related to the incidence of ESBL-producing E. coli.
Incidence of Extended Spectrum Beta-Lactamase (ESBL) of Escherichia Coli Isolated from the Feces of Dairy Cattles in Blitar Dameanti, Fidi Nur Aini Eka Puji; Amri, Indah Amalia; Roosdiana, Anna; Pratama, Alfaro Rikko; Izofani, Safira; Fahmiantika, Reza; Tedja, Dhaneswara; Sutrisno, Rahayu; Akramsyah, Muhammad Ali
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.5

Abstract

Antibiotic resistance (AMR) has caused a serious global threat to human health, animal health and food security. The antibiotic resistance problem, especially in Gram-negative bacteria, is increasingly widespread in various areas and has increased mortality, morbidity, and incidence. This study aims to determine the incidence of ESBL E. coli and the AMR and MDR properties of E.coli isolated from the feces of dairy cattles in Blitar, which have not been reported. This research was conducted from June to September 2022 with 60 samples of dairy cattle feces in Blitar. Samples were isolated and identified until confirmed that 55 (91.6%) samples were E. coli. All samples showed AMR character with 100% streptomycin, 87.27% chloramphenicol, 85.45% ampicillin, 72.73% cefotaxime, 40% tetracycline, 21.82% ciprofloxacin, and 14.55% sulfamethoxazole-trimethoprim. Forty-nine samples were MDR, with 10.9% resistant in three groups, 34.5% in four groups, 29.1% in five groups, 10.9% in six groups, and 3.6% in seven groups. MDR E. coli isolates were then confirmed for ESBL using the DDST test. The incidence of ESBL-producing E. coli from dairy cattle feces in Blitar was 40% (22 isolates). The results of this study can be a warning in the human health, animal health and food security.
Dairy Milk Quality After Foot and Mouth Disease in Ternak Sukses Bersama, Kediri Regency Dameanti, Fidi Nur Aini Eka Puji; Assyifa, Fraya; Kamulyan, Ujiati; Delivery, Rheina Yesi Octa; Safri, M. Ali AkramSyah; Ompusunggu, Meifi Mariana; Sutrisno, Rahayu
Journal of Applied Veterinary Science And Technology Vol. 5 No. 2 (2024): October 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I2.2024.103-108

Abstract

Background: Milk, a nutrient-rich substance obtained from udder secretions, can suffer quality degradation due to factors like disease-causing microorganisms. Foot and Mouth Disease (FMD), an acute viral infection affecting cloven-hoofed animals, can severely impact milk production and quality. Purpose: This study aims to analyze the quality of milk from dairy cattle following the FMD outbreak, as FMD not only reduces milk yield but also alters its composition, affecting consumer preferences and farmer income. Method: Milk quality tests were conducted on cattle following the FMD outbreak with each 50 ml sample stored in an icebox and analyzed using a calibrated Lactoscan tool. The analysis covered fat, solid-non-fat (SNF), density, lactose, salts, protein, temperature, freezing point, added water, total solids, and pH levels. The study received consent from local authorities and breeders. Results: The analysis of cow milk post Foot and Mouth Disease (FMD) outbreak reveals significant variability in milk composition, affecting quality and processing, with many falling below or above standard thresholds for fat content, SNFs, protein content, freezing point, and added water, while all samples had a pH level outside the acceptable range. Conclusion:  Quality of dairy milk from dairy cattle after FMD outbreaks show decreasing of fat content in five milk samples, four samples in solid non fat, three samples in protein, nine samples in freezing point, eight samples in added water, and all samples fell outside the acceptable pH range.
Analisis Kualitas Air Limbah Peternakan Sapi Perah Berdasarkan Nilai Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Ph dan Escherichia Coli di Kabupaten Kediri Dameanti, Fidi Nur Aini; Akramsyah S, Muhammad Ali; Hasan, Chyntia Silvi Yanti; Amanda, Jacky Teguh; Sutrisno, Rahayu
TERNAK TROPIKA Journal of Tropical Animal Production Vol. 23 No. 1 (2022): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2022.023.01.9

Abstract

Peternakan sapi perah mampu menghasilkan produk susu yang bernilai ekonomi tinggi namun juga menghasilkan produk berupa limbah baik cair (air), padat dan gas. Limbah cairan pada peternakan sapi perah didominasi oleh air yang telah digunakan untuk pembersihan, sanitasi, pemanasan, pendinginan, dan pencucian lantai. Air limbah yang langsung dibuang tanpa melalui pengolahan dapat berdampak pada kualitas lingkungan sekitar. Pencemaran air di sekitar lingkungan peternakan sapi perah disebabkan karena kontaminasi dari limbah yang bisa dilihat dari nilai BOD, COD, pH dan keberadaan Escherichia. coli. Desain penelitian yang digunakan adalah penelitian deskriptif laboratorik yang dilakukan pada bulan Juni-September 2021. Pemilihan sampel dilakukan secara purposive sampling sehingga sampel yang diperoleh adalah 8 sampel dari Kelompok Ternak Sukses Bersama, Desa Deyeng, Kabupaten Kediri. Sampel air limbah dilakukan pengujian kadar BOD dan COD di Laboratorium Lingkungan Perum Jasa Tirta 1 dan analisis kadar pH serta deteksi E. coli. dilakukan di Laboratorium Mikrobiologi dan Imunologi Fakultas Kedokteran Hewan, Universitas Brawijaya. Pengujian kadar BOD dengan menggunakan metode APHA 5210B-2017, kadar COD berdasarkan SNI 6989.2:2009, pengujian pH berdasarkan SNI 6989.2:2019, dan deteksi E. coli dilakukan dengan uji penguat atau confirmed test uji kualitatif koliform. Hasil penelitian menunjukkan bahwa nilai BOD berkisar antara 3466 – 18000 mg/L, nilai COD antara 12800 – 66300 mg/L, nilai pH antar 7,3-8,04, dan seluruh sampel positif mengandung E.coli. Hasil tersebut menujukkan bahwa seluruh indikatir berada di atas ambang batas yang ditetapkan sehingga perlu dilakukan pengolahan air limbah yang lebih baik.
Co-Authors Akramsyah S, Muhammad Ali Akramsyah, Muhammad Ali Amanda, Jacky Teguh Amri, Indah Amalia Anna Roosdiana Assyifa, Fraya Dameanti, Fidi Nur Aini Eka Puji Delivery, Rheina Yesi Octa Fahmiantika, Reza Hasan, Chyntia Silvi Yanti Izofani, Safira Kamulyan, Ujiati Ompusunggu, Meifi Mariana Pratama, Alfaro Rikko Safri, M. Ali AkramSyah Tedja, Dhaneswara