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Delaiah, Diena
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Biochemistry, Genetics & Molecular Biology Veterinary

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Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition Delaiah, Diena; Aswin, Ahmad; Susilowati, Helen; Wijaya, Andi Yasmin; Maulana, Firdausy Kurnia; Diyantoro, Diyantoro; Rodprasert, Watchareewan; Puspitasari, Yulianna; Dhamayanti, Yeni; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.1-6

Abstract

Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages including batch to batch variation and adventitious agent. Therefore, chemically defined serum-free media (SFM) are formulated by using standardized growth factors. Epidermal Growth Factor (EGF) is one of growth factors that showed adequate mitogenic support in serum-free medium system, especially in Vero cells. In this study, SFM-EGF media was compared with serum supplementation media, namely MEM 10% and MEM 5% FBS supplementation. The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. Vero E6 and Vero CCL-81 proliferation in SFM-EGF media on day one to four did not show a significant difference compared to MEM 10% or MEM 5% serum supplementation media. However, the OD values of both Vero E6 and Vero CCL-81 cells given SFM-EGF media produced an average value below MEM 10% but higher than MEM 5% FBS supplementation. As such, this study proved that utilizing SFM-EGF could support the proliferation of Vero E6 and Vero CCL-81 cells.
Co-Authors Aswin, Ahmad Diyantoro Diyantoro, Diyantoro Helen Susilowati, Helen Kuncorojakti, Suryo Maulana, Firdausy Kurnia Rodprasert, Watchareewan Wijaya, Andi Yasmin Yeni Dhamayanti Yulianna Puspitasari