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All Journal Jurnal Medik Veteriner Khidmat : Jurnal Pendidikan dan Ilmu Sosial
Aswin, Ahmad
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Biochemistry, Genetics & Molecular Biology Education Social Sciences Veterinary

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Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition Delaiah, Diena; Aswin, Ahmad; Susilowati, Helen; Wijaya, Andi Yasmin; Maulana, Firdausy Kurnia; Diyantoro, Diyantoro; Rodprasert, Watchareewan; Puspitasari, Yulianna; Dhamayanti, Yeni; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.1-6

Abstract

Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages including batch to batch variation and adventitious agent. Therefore, chemically defined serum-free media (SFM) are formulated by using standardized growth factors. Epidermal Growth Factor (EGF) is one of growth factors that showed adequate mitogenic support in serum-free medium system, especially in Vero cells. In this study, SFM-EGF media was compared with serum supplementation media, namely MEM 10% and MEM 5% FBS supplementation. The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. Vero E6 and Vero CCL-81 proliferation in SFM-EGF media on day one to four did not show a significant difference compared to MEM 10% or MEM 5% serum supplementation media. However, the OD values of both Vero E6 and Vero CCL-81 cells given SFM-EGF media produced an average value below MEM 10% but higher than MEM 5% FBS supplementation. As such, this study proved that utilizing SFM-EGF could support the proliferation of Vero E6 and Vero CCL-81 cells.
Penggunaan Permainan Puzzle Huruf Hijaiyah Dalam Pengembangan Kecerdasan Linguistik Anak Usia 7-8 Tahun Aswin, Ahmad
Khidmat Vol 1 No 2 (2023)
Publisher : CV Edu Tech Jaya

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Abstract

Penelitian ini mengemukakan permasalahan tentang kemampuan anak-anak megenal huruf hijayah di kelas 1 SDN 058103 Parangguam masih rendah dalam mengenal huruf hjaiyah tersebut. Penelitian ini bertujuan melalui kegiatan bermain dengan media puzzle huruf hijaiyah diharapkan bahwa peserta didik, terutama anak-anak kelas 1, akan dapat menambah pemahaman mereka tentang huruf hijaiyah. Tidak hanya itu, aktivitas ini pula diharapkan bisa meningkatkan kecerdasan linguistik, spesialnya keahlian bahasa pada kanak- kanak. Metodologi riset yang digunakan merupakan pendekatan kualitatif dengan pendekatan deskriftif, dimana informasi hasil riset yang digunakan berbentuk kalimat, tidak berbentuk angka. Riset ini dicoba di SDN 058103 Parangguam. Hasil riset bahwasanya pemakaian game media puzzle bisa signifikan tingkatkan keahlian anak dalam memahami huruf hijaiyah di kelas 1 SDN 058103 Parangguam. Selain itu, media ini juga berpotensi untuk mengembangkan kecerdasan linguistik anak, karena pemahaman huruf hijaiyah memungkinkan mereka untuk mengucapkan bunyi-bunyi huruf tersebut. Dengan demikian, perkembangan bahasa pada anak dapat lebih terlatih dan berkembang dengan baik.
Evaluation and Its Impact of SARS-CoV-2 Inactivated Vaccine Candidate in K18-hACE2 Mice A'la, Rofiqul; Rantam, Fedik Abdul; Wijaya, Andi Yasmin; Susilowati, Helen; Kuncorojakti, Suryo; Diyantoro, Diyantoro; Aswin, Ahmad; Rahmahani, Jola; Suwanti, Lucia Tri; Lukiswanto, Bambang Sektiari; Yudaniayanti, Ira Sari; Setiawan, Boedi
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.54-62

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 requires effective vaccines to be developed. This study aimed to assess the impact of a SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice by monitoring their body weight, immune activation, and inflammatory cytokines including IL-4, IL-6, TNF-α, and IFN-γ. The study utilized k18-hACE2 mice expressing the human angiotensin-converting enzyme 2 (hACE2) receptor. The mice were administered the inactivated vaccine candidate compared with sham and vehicle. Body weight was monitored, and serum samples were collected to measure IL-4, IL-6, TNF-α, and IFN-γ levels using ELISA. Data were evaluated using SPSS statistical analysis software. The administration of the SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice did not result in significant changes in body weight compared to the control group. Furthermore, the levels of IL-4, IL-6, TNF-α, and IFN-γ were significantly reduced in the vaccinated mice compared to the control group, suggesting a dampening effect on the inflammatory response. This study demonstrates that the SARS-CoV-2 inactivated vaccine candidate has a minimal impact on the body weight of k18-hACE2 mice. Nevertheless, it successfully regulates the levels of IL-4, IL-6, TNF-α, and IFN-γ, suggesting its safety and beneficial impact. These findings contribute to understanding the vaccine's efficacy and safety profile in vaccine development.
Novel Surface Marker for the Prospective Mesenchymal Stem Cell Characterization from Rabbit Visceral Adipose Tissue Kuncorojakti, Suryo; Yudaniayanti, Ira Sari; Susanti, Lina; Susilawati, Helen; Aswin, Ahmad; Diyantoro, Diyantoro; Rodprasert, Watchareewan
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.1-9

Abstract

Rabbit as a laboratory animals play an important role in bridging basic research to clinical application. The exploration of rabbit mesenchymal stem cell (MSC) is still facing an obstacle regarding the standardization of characterization of MSC. This study is aimed to explore the novel candidate of rabbit MSC surface marker as an effort to establish the goal standardization of rabbit MSC. Three 2-month-old male White New Zealand rabbits weighing 2 kg were used as visceral rabbit adipose derived mesenchymal stem cell (Rab-ADMSC) donors. The cells were characterized according to their morphology characteristic, clonogenic and three-lineage differentiation capacity. Flow cytometry was used to evaluate the MSC surface markers of Rab-ADMSC against CD81, CD29, CD34 and CD45. The results of this study revealed that the Rab-ADMSC posses characteristic of MSC according to their morphology and differentiation capacity. It can be concluded that CD81 surface marker can be proposed as a stable alternative candidate marker for rabbit MSCs. This data is supported by other phenotypic characteristics of MSCs, both in morphology and in the ability of visceral Rab-ADMSCs to differentiate into adipogenic, osteogenic, and chondrogenic lineages.
Co-Authors A'la, Rofiqul Bambang Sektiari Lukiswanto Boedi Setiawan Delaiah, Diena Diyantoro Diyantoro, Diyantoro Fedik Abdul Rantam Helen Susilowati, Helen Ira Sari Yudaniayanti Kuncorojakti, Suryo LINA SUSANTI Lucia Tri Suwanti, Lucia Tri Maulana, Firdausy Kurnia Rahmahani, Jola Rodprasert, Watchareewan Susilawati, Helen Wijaya, Andi Yasmin Yeni Dhamayanti Yulianna Puspitasari