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All Journal Jurnal Medik Veteriner Jurnal Pengabdian dan Peningkatan Mutu Masyarakat (JANAYU)
Maulana, Firdausy Kurnia
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Religion Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Social Sciences Veterinary Other

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Education and Cadre in Improving Knowledge of One Health in Elementary School-Age Children Sari, Siti Shofiya Novita; Saputro, Sigit Ari; Qurniyawati, Eny; Yamani, Laura Navika; Astutik, Erni; Maulana, Firdausy Kurnia; Maghfiroh, Lailatul
Jurnal Pengabdian dan Peningkatan Mutu Masyarakat (Janayu) Vol. 5 No. 2 (2024): Jurnal Pengabdian dan Peningkatan Mutu Masyarakat
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/janayu.v5i2.30955

Abstract

Purpose – Elementary school students can become agents of change so that education on the concept of One Health. The purpose of this community service is to increase knowledge about the concept of One Health in students and the regeneration of Little One Health Ambassadors at SDN Kalisari II Surabaya City. Design/methodology/approach – The method was counseling on the concept of One Health, and the selection of Little One Health Ambassadors. Activities were carried out in August-September 2023. The educational material was from One Health Lessons and have been adapted for children aged 8 - 10 years. One Health Ambassadors were selected based on the results of the assessment of poster work about One Health, and mastery of One Health material. Counseling activities on One Health education were carried out in the fourth and fifth grades with a total of 80 students. Findings – The results showed that the education provided can improve the knowledge of the target group about the concept of One Health (p-value = 0.003). The first winner of One Health ambassadors was chosen who indicated that he mastered the material about One Health and his work was the most interesting and could present it well. Originality/value – Community service activities produce changes that are by external targets.
Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition Delaiah, Diena; Aswin, Ahmad; Susilowati, Helen; Wijaya, Andi Yasmin; Maulana, Firdausy Kurnia; Diyantoro, Diyantoro; Rodprasert, Watchareewan; Puspitasari, Yulianna; Dhamayanti, Yeni; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.1-6

Abstract

Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages including batch to batch variation and adventitious agent. Therefore, chemically defined serum-free media (SFM) are formulated by using standardized growth factors. Epidermal Growth Factor (EGF) is one of growth factors that showed adequate mitogenic support in serum-free medium system, especially in Vero cells. In this study, SFM-EGF media was compared with serum supplementation media, namely MEM 10% and MEM 5% FBS supplementation. The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. Vero E6 and Vero CCL-81 proliferation in SFM-EGF media on day one to four did not show a significant difference compared to MEM 10% or MEM 5% serum supplementation media. However, the OD values of both Vero E6 and Vero CCL-81 cells given SFM-EGF media produced an average value below MEM 10% but higher than MEM 5% FBS supplementation. As such, this study proved that utilizing SFM-EGF could support the proliferation of Vero E6 and Vero CCL-81 cells.
Co-Authors Astutik, Erni Aswin, Ahmad Delaiah, Diena Diyantoro Diyantoro, Diyantoro Eny Qurniyawati Helen Susilowati, Helen Kuncorojakti, Suryo LAILATUL MAGHFIROH Laura Navika Yamani Rodprasert, Watchareewan Sari, Siti Shofiya Novita Sigit Ari Saputro Wijaya, Andi Yasmin Yeni Dhamayanti Yulianna Puspitasari