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All Journal Jurnal Veteriner Journal Of Vocational Health Studies Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Darmabakti Cendekia: Journal of Community Service and Engagements Journal of Applied Veterinary Science and Technology
Diyantoro Diyantoro, Diyantoro
Department Of Health, Faculty Of Vocational Studies, Universitas Airlangga, Surabaya-Indonesia
Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Seroprevalensi dan Faktor Risiko Penularan Mycoplasma gallisepticum pada Peternakan Ayam Petelur Komersial di Kabupaten Blitar (SEROPREVALENCE AND( RISK FACTORS OF MYCOPLASMA GALLISEPTICUM INFECTION IN COMMERCIAL LAYER FARM IN BLITAR DISTRICT) Diyantoro Diyantoro; I Wayan Teguh Wibawan; Eko Sugeng Pribadi
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.237 KB) | DOI: 10.19087/jveteriner.2017.18.2.211

Abstract

This study aimed to figure out the prevalence and risk factors of Mycoplasma gallisepticum (MG) infection in commercial layer farm in Blitar District. Blood samples and questionnaires were taken during December 2014 to February 2015. A total of 264 sera samples were collected from 22 commercial layer farm. Based on serum plate agglutination test, 26 sera samples were MG positive that were indicated an infection prevalence was 9.85%. The highest SRR value for MG infection was occurred in Bakung Subdistrict (SRR = 2.5). Based on Analysis of multivariate logistic regression showed that a very significant influenced risk factors of MG infection have occurred in flocking density more than 3,000 birds per flock (?2= 11.10; p= 0.001; OR= 6.1), flocking density about 1,501 to 3,000 birds per flock (?2 = 11.10; p= 0.001; OR= 6.1), bird feeding once a day (?2= 9.32; p= 0.002; OR= 0.3), house desinfection once in every two weeks (?2 =7.70; p= 0.009; OR= 1.2), house desinfection once a month or only in case (?2= 9.36; p= 0.006; OR= 3.9). It was concluded that seroprevalence of MG infection in studied area was 9,85%. the MG seroprevalence were influenced by flocking density more than 3,000 birds per flock, flocking density about 1,501 to 3,000 birds per flock, bird feeding once a day, house desinfection once every two weeks, and house desinfection once a month or only in case. ABSTRAK Mycoplasmosis merupakan salah satu penyakit paling penting yang dihadapi oleh industri perunggasan di Indonesia. Penelitian ini bertujuan untuk mengetahui prevalensi dan faktor risiko penularan Mycoplasma gallisepticum (MG) pada peternakan ayam petelur komersial di Kabupaten Blitar, Jawa Timur. Contoh darah dan kuisioner diambil selama periode Desember 2014 hingga Februari 2015. Sebanyak 264 contoh serum dikumpulkan dari 22 peternakan ayam petelur komersial. Berdasarkan uji Rapid Serum Agglutination (RSA), 26 contoh ditemukan positif MG dengan prevalensi infeksi MG di Kabupaten Blitar sebesar 9,85%. Nilai rasio risiko terbakukan (standardized risk ratio, SRR) untuk penularan MG paling tinggi adalah Kecamatan Bakung (SRR = 2,5). Hasil analisis regresi logistik multivariate didapatkan bahwa faktor yang berpengaruh sangat nyata (P<0,01) terhadap terjadinya infeksi MG adalah kepadatan flok di atas 3.000 ekor ( ?2= 11,10; p= 0,001; OR= 6,1), kepadatan flok 500-1.500 ekor (?2 = 11,09; p= 0,004; OR= 3,4), pemberian pakan satu kali dalam sehari (?2= 9,32; p= 0,002; OR=0,3), penyemprotan kandang yang dilakukan satu kali dalam dua minggu (?2 = 7,70; p= 0,009; OR= 1,2),dan penyemprotan kandang yang hanya dilakukan satu kali dalam sebulan atau hanya jika terjadi kasus ?2 ( = 9,36; p= 0,006; OR= 3,9). Hasil penelitian dapat disimpulkan bahwa prevalensi infeksi MG di daerah kajian adalah 9,85%. Faktor risiko yang berpengaruh sangat nyata terhadap penularan MG antara lain jumlah ayam yang dipelihara di atas 3.000 ekor per kandang, jumlah ayam yang dipelihara sebanyak 1.501- 3.000 ekor per kandang, pemberian pakan satu kali sehari, penyemprotan kandang satu kali dalam dua minggu, dan penyemprotan kandang satu kali sebulan atau hanya jika ada kasus. Mycoplamosis is one of an important poultry diseases that should be well managed by poultry farmer in Indonesia.
ANALYSIS OF THE INCIDENT RATE OF PEOPLE WITH A POTENTIAL RISK FOR HYPERTENTION AND DIABETES IN KENJERAN SUB-DISTRICT, SURABAYA Maya Septriana; Aliyah Siti Sundari; Diyantoro Diyantoro; Dwi Wahyu Indriati; Anita Kurniati; Ni Nyoman Purwani; Tofan Agung Eka Prasetya; Fadilatus Sukma Ika Noviarni; Shelly Wulandari; Myrna Adianti
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 1 No. 1 (2019): June 2019
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.389 KB) | DOI: 10.20473/dc.V1.I1.2019.11-15

Abstract

Background: The number of people with diabetes mellitus in Indonesia is increasing every year. The data in 2016 showed that 1 of 11 adults could have diabetes mellitus. Uncontrolled and untreated diabetes mellitus can cause complications. Objectives: This activity aimed to determine the group of age that have a potential risk of hypertension and diabetes so that it could minimize the prevalence of disease and optimize human productivity. Methods: (1) Counseling about hypertension and diabetes, (2) Screening the people with a potential risk of hypertension using tensimeter and diabetes prediction using point-of-care testing (POCT) and urine dipstick. Results: The data were collected from 75 respondents with characteristics about 85,33% female and 14,67% male. About 25 respondents (33,33%) were suspected of hypertension. Based on age group, it showed that the highest incident rate of hypertension was found in the age group of 41-60 years (41,86%), followed by the age group of 61-80 years (20%), 21-40 years (13,33%), and age under 20 years and above 80 years (0%). The majority of respondents had a normal glucose level(90,67%). Based on gender, there was 1 male respondent (9,09%)and5female respondents  (7,81%) having a glucose level higher than normal value (hyperglycemia). Conclusion:  The age group with the highest potential risk for hypertension was 41-60 years, while for diabetes was 61-80 years. This activity provided information to prevent the potential risk leading to hypertension and diabetes.
PUBLIC AWARENESS ABOUT SYMPTOMS AND PREVENTION OF COVID-19 IN BENOWO, SURABAYA Dwi Wahyu Indriati; Diyantoro Diyantoro; Anita Kurniati; Aliyah Siti Sundari; Rizka Oktarianti Ainun Jariah; Nur Septia Handayani
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 3 No. 2 (2021): DECEMBER 2021
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.478 KB) | DOI: 10.20473/dc.V3.I2.2021.61-65

Abstract

Background: Since its first report, COVID-19 has remained a major threat worldwide. Various attempts were made to treat and prevent further transmission of COVID-19. In Indonesia, although the first case was reported nearly five months after the first case in Wuhan, the transmission was the fastest among Southeast Asian countries. Several efforts have been made to prevent further transmission. Objective: Our community services program aimed to assess the public understanding of COVID-19 symptoms and the application of SHE (Self and Environmental Health) as a form of COVID-19 prevention. Method: We conducted counseling using a door-to-door method to increase the understanding of SHE as a form of COVID-19 prevention and then at the end of the visit, a questionnaire was given to measure the understanding of symptoms and prevention COVID-19 transmission. Results: After conducted the door-to-door counselling in Kandangan district, it is known that the public understanding of the symptoms and ways of preventing COVID-19 is quite good. The method of prevention that is understood by the public includes using masks, diligently wash hands, and maintain social distance in gathering. However, there are still people who do not understand (31.13%) that increasing their immune system by consuming nutritious and balanced food and exercise can also be used to prevent the transmission of COVID-19. Because a good immune system can prevent individuals from infection with pathogens such as COVID-19. Conclusion: The community has sufficient understanding of the symptoms and ways of preventing COVID-19 so that it is expected it can reduce the transmission of COVID-19 in Benowo District, Surabaya.
A Case Study of Feline Panleukopenia in Cats at The Educational Animal Hospital of Universitas Airlangga Yusril Nur Mahendra; M. Gandul Atik Yuliani; Agus Widodo; Diyantoro Diyantoro; Miyayu Soneta Sofyan
Journal of Applied Veterinary Science And Technology Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V1.I1.2020.6-10

Abstract

Background: Feline panleukopenia is a viral disease caused by the family of Parvoviridae. Many species of pets, especially cats, that if they have been infected, compromised health and can lead to death. Purpose: To determine the infection pattern and treatment of feline panleukopenia in cats at the Educational Animal Hospital of Universitas Airlangga. Method: Ten patients were recorded as infected with parvovirus from a total of 1107 patients with various disease complaints. Daily records were taken regarding all clinical symptoms, physical examination, and treatment. Result: After four weeks, the obtained data were calculated. Thus, we collect data that show essential factors that can be used to determine the diagnose of feline panleukopenia in cats. Conclusion: Most parvovirus infections occurred in cats aged two to four years by showing abnormalities in temperature, breathing, and turgor. Clinical symptoms that appeared include vomiting, diarrhea, and high dehydration. Treatments were used to suppress the secondary effects of parvovirus infection and besides to eradicate the virus itself. 
Detection of Salmonella sp. on Layer Chicken Eggs Sold at The Vegetable Market of Magetan Regency Brillian Inawulan Syamsiar; Kuncoro Puguh Santoso; Ira Sari Yudaniayati; Diyantoro Diyantoro
Journal of Applied Veterinary Science And Technology Vol. 1 No. 2 (2020): October 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V1.I2.2020.34-38

Abstract

Background: Eggs are poultry products that cannot be separated from the possibility of Salmonella contamination. Traditional markets are places where consumers can get chicken eggs with poor hygiene and sanitation aspects. Habits of today's society that often consumes raw or undercooked eggs can trigger Salmonellosis. Purpose: To identify the presence of Salmonella sp. in eggs sold in Magetan Regency Vegetable Market. Method: Sampling of eggs from different random stores. The eggs then proceeded with selection pre-enrichment, selective enrichment, isolation, and identification. Results: The handling of eggs in the Magetan Regency Market was quite good in its storage period, and purebred eggs sold in the Magetan Regency Market were negatively contaminated with Salmonella sp. Conclusion: Broiler chicken eggs sold in Magetan Vegetable Market are safe to consume.
The Effect of Providing Fermented Milk on The Performance of Gallus domesticus Dalila Fadhila Hidayat; Agus Widodo; Diyantoro Diyantoro; M. Gandul Atik Yuliani
Journal of Applied Veterinary Science And Technology Vol. 1 No. 2 (2020): October 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V1.I2.2020.43-47

Abstract

Backgrounds: Broiler chickens are high productivity livestock. To achieve this productivity, many farmers provide feed additives. Fermented milk is one of the feed additives given through drinking water. Providing feed additives, regularly, will affect the performance of these broilers. Purpose: To investigate the potential of providing fermented milk through drinking water on the performance of broiler chickens. Method: Direct observations and perform calculations on several aspects that support the research. Result: After making observations, it was known that there are differences in the starter phase and the finisher phase. The observation was carried out from the age of 0-27 days. Broiler performance determined by the parameters of feed consumption, chicken body weight, and feed conversion. Conclusion: The observation shows an increase in feed consumption. However, in feed conversion and body weight gain instability is caused by physical conditions, temperature, and palatability in each phase of the broiler chickens.
Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition Delaiah, Diena; Aswin, Ahmad; Susilowati, Helen; Wijaya, Andi Yasmin; Maulana, Firdausy Kurnia; Diyantoro, Diyantoro; Rodprasert, Watchareewan; Puspitasari, Yulianna; Dhamayanti, Yeni; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.1-6

Abstract

Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages including batch to batch variation and adventitious agent. Therefore, chemically defined serum-free media (SFM) are formulated by using standardized growth factors. Epidermal Growth Factor (EGF) is one of growth factors that showed adequate mitogenic support in serum-free medium system, especially in Vero cells. In this study, SFM-EGF media was compared with serum supplementation media, namely MEM 10% and MEM 5% FBS supplementation. The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. Vero E6 and Vero CCL-81 proliferation in SFM-EGF media on day one to four did not show a significant difference compared to MEM 10% or MEM 5% serum supplementation media. However, the OD values of both Vero E6 and Vero CCL-81 cells given SFM-EGF media produced an average value below MEM 10% but higher than MEM 5% FBS supplementation. As such, this study proved that utilizing SFM-EGF could support the proliferation of Vero E6 and Vero CCL-81 cells.
ANALYSIS OF MYCOPLASMA GALLISEPTICUM INFECTION FACTORS IN COMMERCIAL LAYER CHICKEN FARM USING ANALYTICAL HIERARCHY PROCESS Diyantoro, Diyantoro; Pribadi, Eko Sugeng
Journal of Vocational Health Studies Vol. 1 No. 2 (2017): November 2017 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V1.I2.2017.44-49

Abstract

Background: Mycoplasma galliseptium (MG) is a pathogenic microbial often found in poultry farm industry. In Indonesia there are rarely data of MG research. There are some factors increased the risk of MG infection. Purpose: This research aimed to determine the factors involved in the transmission of MG in commercial layer chicken farm. Method: This was a descriptive research with the data was collected from pairwise comparison questionnaire distributed to poultry expert. The Data was analyzed using Analytical Hierarchy Process (AHP) supported by computer programme "Expert Choice”. Result: The results showed that risk value of rearing management (0.425), health management (0.312), feed management (0.088), and biosecurity of  farm (0.175) to the transmission of MG. The results of AHP analysis to the risk factor variables of MG infection in commercial layer chicken farm showed that the highest risk value of MG infection was the number of bird in house (0.277), followed by antibiotic administration (0.276), while the other variables only have risk value under 0.100. Conclusion: The highest risk value of MG infection was rearing management factor especially on the variable of the population number of poultry in house.
PROFILE OF HIV/AIDS PATIENTS COINFECTED WITH TUBERCULOSIS IN IBNU SINA DISTRICT HOSPITAL, GRESIK,EAST JAVA, INDONESIA Widyaningrum, Lilik N.; Indriati, Dwi W.; Diyantoro; Sundari, Aliyah Siti
Journal of Vocational Health Studies Vol. 6 No. 2 (2022): November 2022 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V6.I2.2022.102-106

Abstract

Background: HIV/AIDS cases continue to increase globally. A person with a weak immune system, especially people with HIV/AIDS, is very susceptible to various diseases, such as tuberculosis. Tuberculosis infection can be associated with HIV-positive individuals, which is caused by damage to cellular immunity due to the reduced number and function of CD4 cells. Purpose: To determine the profile of HIV/AIDS patients with TB coinfection based on CD4 values in RSUD Ibnu Sina Gresik. Method: The method used is observational analytical cross-sectional. Patients with TB and HIV coinfection were recapitulated from the Case Report Form (CRF). The results are presented in a frequency distribution table. Result: The study obtained information about 36 people with TB co-infected HIV/AIDS cases. Patients with CD4 values <200 cells/mm3 were 35 people (97.22%), and most of them were in the age group of 20-60 years were 35 people (97.22%). Male patients (83.33 %) dominate compared to female patients (16.67%). The duration of TB coinfection in HIV/AIDS patients occurs in less than one month, which is 21 people (58.33%). Conclusion: The profile of HIV/AIDS patients co-infected with TB is based on CD4 values that are more dominant in men, with the highest age being 20 - 60 years.
Evaluation and Its Impact of SARS-CoV-2 Inactivated Vaccine Candidate in K18-hACE2 Mice A'la, Rofiqul; Rantam, Fedik Abdul; Wijaya, Andi Yasmin; Susilowati, Helen; Kuncorojakti, Suryo; Diyantoro, Diyantoro; Aswin, Ahmad; Rahmahani, Jola; Suwanti, Lucia Tri; Lukiswanto, Bambang Sektiari; Yudaniayanti, Ira Sari; Setiawan, Boedi
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.54-62

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 requires effective vaccines to be developed. This study aimed to assess the impact of a SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice by monitoring their body weight, immune activation, and inflammatory cytokines including IL-4, IL-6, TNF-α, and IFN-γ. The study utilized k18-hACE2 mice expressing the human angiotensin-converting enzyme 2 (hACE2) receptor. The mice were administered the inactivated vaccine candidate compared with sham and vehicle. Body weight was monitored, and serum samples were collected to measure IL-4, IL-6, TNF-α, and IFN-γ levels using ELISA. Data were evaluated using SPSS statistical analysis software. The administration of the SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice did not result in significant changes in body weight compared to the control group. Furthermore, the levels of IL-4, IL-6, TNF-α, and IFN-γ were significantly reduced in the vaccinated mice compared to the control group, suggesting a dampening effect on the inflammatory response. This study demonstrates that the SARS-CoV-2 inactivated vaccine candidate has a minimal impact on the body weight of k18-hACE2 mice. Nevertheless, it successfully regulates the levels of IL-4, IL-6, TNF-α, and IFN-γ, suggesting its safety and beneficial impact. These findings contribute to understanding the vaccine's efficacy and safety profile in vaccine development.
Co-Authors A'la, Rofiqul Agung Budianto Achmad, Agung Budianto Agus Widodo Agus Widodo Anita Kurniati Aswin, Ahmad Bambang Sektiari Lukiswanto Boedi Setiawan Brillian Inawulan Syamsiar Chrismanto, Dony Dalila Fadhila Hidayat Delaiah, Diena Dwi Wahyu Indriati Eko Sugeng Pribadi Fadilatus Sukma Ika Noviarmi Fedik Abdul Rantam Gandul Atik Yuliani Helen Susilowati, Helen I wayan Teguh Wibawan Indriati, Dwi W. Ira Sari Yudaniayanti Ira Sari Yudaniayati Kuncoro Puguh Santoso Kuncorojakti, Suryo LINA SUSANTI Lucia Tri Suwanti, Lucia Tri Maulana, Firdausy Kurnia Meilinia, Shafina Meilinia Myrna Adianti Ni Nyoman Purwani Nur Septia Handayani Prasetya, Tofan Agung Eka Rahmahani, Jola Rizka Oktarianti Ainun Jariah Rodprasert, Watchareewan Septriana, Maya Sofyan, Miyayu Soneta Sundari, Aliyah Siti Susilawati, Helen Widyaningrum, Lilik N. Wijaya, Andi Yasmin Wulandari, Shelly Yeni Dhamayanti Yulianna Puspitasari Yusril Nur Mahendra