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All Journal Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan JURNAL KAJIAN VETERINER Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Kontribusi: Jurnal Penelitian dan Pengabdian Kepada Masyarakat Current Biomedicine Jurnal Agro Veteriner
Yulianna Puspitasari
Divisi Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlannga, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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PENYULUHAN MASTITIS SUBKLINIS DAN KEGIATAN UJI CALIFORNIA MASTITIS TEST PADA SAPI PERAH DI KOPERASI UNIT DESA KECAMATAN NGANCAR, KABUPATEN KEDIRI, JAWA TIMUR Yulianna Puspitasari; Wiwiek Tyasningsih; Jola Rahmahani; Suwarno Suwarno; Sri Chusniati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 5 No. 2 (2021): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v5i2.2021.489-495

Abstract

AbstractSubclinical mastitis is an inflammation of the udder that often attacks livestock, especially milk-producing livestock. The impact of the subclinical mastitis losses is quite large, including a drastic decrease in the quantity and quality of milk, but no pathological changes were found in cows. The Karya Bhakti Village Unit Cooperative (VUC) is a center for milk production in the Kediri Regency which accommodates dairy cows' milk products from people's farms in the Kediri Regency. The low awareness of farmers in the management of livestock health care and hygiene results in low productivity and quality of milk and the high incidence of subclinical mastitis. This encourages counseling about subclinical mastitis. The socialization method used for counseling were carried out by presenting material about subclinical mastitis with lecture techniques (power point slides), videos about the method of early detection of subclinical mastitis using California Mastitis Test (CMT), conducting demonstrations of testing milk samples using the CMT method and discussion about the material that has been delivered as an indicator of the success of counseling. This series of counseling activities is an effort to reduce the incidence of subclinical mastitis through increasing public knowledge and understanding. This activity also has a positive impact by reducing infectious diseases, especially subclinical mastitis in livestock so that the health of livestock and products from livestock will also be guaranteed both in terms of quality and quantity.Keywords: mastitis, subclinical mastitis, cows, Counseling, CMTAbstrakMastitis subklinis merupakan radang ambing yang sering menyerang hewan ternak khususnya ternak penghasil susu. Dampak kerugian mastitis subklinis diantaranya penurunan kuantitas dan kualitas susu secara drastis, namun tidak ditemukan adanya perubahan patologis pada sapi.  Koperasi Unit Desa (KUD) Karya Bhakti merupakan KUD yang menjadi sentra penghasil susu di Wilayah Kabupaten Kediri yang menampung hasil susu sapi perah dari peternakan – peternakan rakyat di Wilayah Kabupaten Kediri. Rendahnya kesadaran peternak dalam manajemen kesehatan ternak dan hiegienitas kandang mengakibatkan rendahnya produktivitas dan kualitas susu serta masih tingginya angka kejadian mastitis subklinis.  Angka kejadian mastitis subklinis yang tinggi ini mendorong untuk dilakukan penyuluhan mengenai mastitis subklinis. Metode sosialisasi yang digunakan untuk penyuluhan adalah menggunakan alat peraga berupa slide power point yang berisikan materi mastitis subklinis, video metode deteksi dini mastitis subklinis menggunakan California Mastitis Test(CMT), demonstrasi CMT pada sampel susu serta tanya jawab seputar materi yang telah disampaikan sebagai salah satu indikator keberhasilan penyuluhan. Rangkaian kegiatan penyuluhan ini sebagai upaya untuk menekan angka kejadian mastitis subklinis melalui peningkatan pengetahuan dan pemahaman masyarakat. Kegiatan ini juga memberikan dampak positif dengan menurunnya penyakit infeksi khususnya mastitis subklinis pada hewan ternak sehingga kesehatan hewan ternak dan produk asal hewan ternak juga akan terjamin baik dari segi kualitas maupun kuantitasnya.Kata Kunci: mastitis, mastitis subklinis, sapi perah, penyuluhan, CMT
Penerapan Video Edukasi untuk Meningkatkan Pemahaman Penggunaan Ramuan Herbal Selama Pandemi Covid-19 bagi Kelompok Remaja Kecamatan Loceret Kabupaten Nganjuk Agus Muji Santoso; Guruh Sukma Hanggara; Zainal Afandi; Endang Sri Mujiwati; Heru Budiono; Poppy Rahmatika Primandiri; Irwan Setyowidodo; Aan Nurfahrudianto; Elis Irmayanti; Siti Aizah; Ericka Darmawan; Rizhal Hendi Ristanto; Bea Hana Siswati; Mohamad Amin; Yulianna Puspitasari; Tri Julianto
Kontribusi: Jurnal Penelitian dan Pengabdian Kepada Masyarakat Vol. 1 No. 2 (2021): Mei 2021
Publisher : Cipta Media Harmoni

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1269.346 KB) | DOI: 10.53624/kontribusi.v1i2.34

Abstract

Ramuan herbal dapat berpotensi besar untuk meningkatkan ketahanan tubuh dalam masa pandemi jika digunaan secara tepat. Rendahnya pengetahuan tentang takaran, teknik pengolahan, pola konsumsi ramuan herbal, dan efek sampingnya menjadikan fokus untuk program pengabdian masyarakat dengan memberikan informasi yang komprehensif tentang penggunaan ramuan herbal yang dapat meningkatkan daya tahan tubuh secara baik dan benar. Program pemberdayaan masyarakat dilaksanakan dalam moda daring melalui media jejaring sosial secara asinkronus sesuai tahap ODSIMED (observasi, pengembangan, sosialisasi, implementasi, monitoring, evaluasi, diseminasi) Program ini mampu meningkatkan  pengetahuan masyarakat sasaran tentang penggunaan ramuan herbal yang tepat. Khususnya tentang teknik pengolahan ramuan herbal (skor N-Gain 0,77).
Identification and Prevalence of Endoparasite on Layer Chicken in Udanawu Sub-district Blitar Khalissa Farah Alifia; Setiawan Koesdarto; Yulianna Puspitasari; Mufasirin; Adiana Mutamsari Witaningrum
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.38769

Abstract

The aim of this research was to determined the prevalence and infection degrees of endoparasite on layer chicken in Sub-district Udanawu, Blitar. Ninety-six samples were taken from layer chicken in 3 different villages namely Bakung Village, Tunjung Village, and Slemanan Village. The examinations taken in this study are fecal examination using native, sediment, and floating methods and blood examination using blood smear method. Result showed that 81.25% samples are positive for helminthiasis infection consisting of Ascaridia galli (66.67%), Heterakis gallinarum (45.83%), Raillietina sp. (31.25%), and Strongyloides avium (7.29%). Blood examination result shown there is no positive sample that infect layer chicken in Sub-district Udanawu, Blitar. Chi-Square test result showed there are significant difference (P<0.05) of Ascaridia galli and Heterakis gallinarum in Bakung Village, Tunjung Village, and Slemanan Village in Sub-district Udanawu, meanwhile there are no significant difference (P>0.05) of Raillietina sp. and Strongyloides avium. Range of infection degrees of helminthiasis in Bakung village, Tunjung village, and Slemanan Village are 608.75 ± 588.53, 223.12 ± 359.21, 156.25 ± 332.39. There are significant difference (P<0.05) on helminthiasis infection degree of layer chicken in Udanawu District, Blitar.
Hubungan teknik pemerahan dengan jumlah Escherichia coli pada susu segar dari peternakan sapi perah di KUD Kertajaya, Kabupaten Kediri, Jawa Timur Rahadyan, Raihan Alif; Tyasningsih, Wiwiek; Puspitasari, Yulianna; Permatasari, Dian Ayu; Widjiati, Widjiati
Current Biomedicine Vol. 1 No. 1 (2023): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.1.19-24

Abstract

Background: The coliform bacteria group is a group of bacteria used as an indicator of food sanitation, one of which is in dairy products. Objective: This study aimed to determine the relationship of milking techniques with the number of Escherichia coli in raw milk compared to the bacterial contamination limit set by the National Standardization Agency. Methods: The design of this study was a descriptive study. Raw milk samples were taken from Lucky Farm and several traditional dairy farms in the KUD Kertajaya area, Kediri Regency, East Java. This study uses the most probable number (MPN) test. Results: The results showed 11 (73.3%) of raw milk samples using conventional milking techniques exceeding the maximum limit of E. coli bacterial contamination determined by the National Standardization Agency (< 3 MPN/mL). However, all 15 samples, or 100% of raw milk samples using machine milking techniques, were negative for E. coli and met the maximum bacterial contamination limit requirements. Conclusion: The milking techniques affect E. coli contamination limits in raw milk. The milk samples taken using machine milking techniques had better results with MPN values ​​< 3 MPN/mL than those taken using conventional milking techniques.
Total plate count dalam isi telur ayam ras di pasar tradisional Kecamatan Waru, Kabupaten Sidoarjo Budiarto, Budiarto; Kirana, Sabila Citra; Lestari, Tita Damayanti; Budiarto; Puspitasari, Yulianna; Mutamsari Witaningrum, Adiana; Permatasari, Dian Ayu
Current Biomedicine Vol. 1 No. 2 (2023): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.2.86-94

Abstract

Background: Microbial contamination in consumed chicken eggs can endanger human health. Bacteria can grow and develop in food so that it can reduce the food quality. Determination of food quality can be done by various methods, one of which is Total Plate Count (TPC). Objective: The aim of this study was to determine the level of bacterial contamination in chicken eggs sold at traditional markets in Waru District, Sidoarjo Regency. Methods: Thirty samples were collected from two traditional markets, Waru Market and Wadung Asri Market. This study used the pour plate method of TPC test. Egg contents were diluted and then inoculated in Nutrient Agar. The media inoculated with the sample suspension was incubated at 37°C for 24 hours. The growing bacterial colonies were counted and analyzed using the Standard Plate Count. This study's data are presented descriptively and compared with the Indonesian National Standard (SNI) 7388-2009 maximum requirement of contaminant bacteria in chicken eggs is 1×105 CFU/mL. Results: The results showed that the averages TPC in two traditional markets were 2.1×104 CFU/mL in Waru Market and 3.4×103 CFU/mL in Wadung Asri Market. The average TPC still meets the maximum limit of microbial contamination listed in SNI 7388-2009. Conclusion: The average values of TPC in both markets are not the same, but the qualities of chicken eggs sold in Waru Market and Wadung Asri Market are good and suitable for consumption.
Identification of Escherichia coli Multidrug Resistance in Cattle in Abattoirs Mustika, Yolla Rona; Effendi, Mustofa Helmi; Puspitasari, Yulianna; Plumeriastuti, Hani; Khairullah, Aswin Rafif; Kinasih, Kurnia Nisa
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.19-32

Abstract

Escherichia coli is a typical flora of an animals' digestive tract. Based on these details, this study was done on the detection and identification of multidrug-resistant E. coli in cattle in Surabaya abattoirs. Each cattle rectum swab sample was streaked into EMB agar media and followed by Gram staining. The IMViC test was used to confirm the presence of E. coli. Based on morphological culture features, Gram staining, and biochemical testing, the sample examination results revealed that 41 samples (41%) of the 100 cattle rectal swab samples that were isolated were confirmed to be positive for E. coli. The profile of antibiotic resistance from the results of the E. coli resistance test to antibiotics showed that out of a total of 41 isolates of E. coli, 12 isolates (29.27%) were detected as resistant to 1 class of antibiotics tested, while 2 isolates (4.88%) were resistant to 2 classes of antibiotics, and 3 isolates (7.32%) were confirmed to be multidrug resistance (MDR).
Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition Delaiah, Diena; Aswin, Ahmad; Susilowati, Helen; Wijaya, Andi Yasmin; Maulana, Firdausy Kurnia; Diyantoro, Diyantoro; Rodprasert, Watchareewan; Puspitasari, Yulianna; Dhamayanti, Yeni; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.1-6

Abstract

Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages including batch to batch variation and adventitious agent. Therefore, chemically defined serum-free media (SFM) are formulated by using standardized growth factors. Epidermal Growth Factor (EGF) is one of growth factors that showed adequate mitogenic support in serum-free medium system, especially in Vero cells. In this study, SFM-EGF media was compared with serum supplementation media, namely MEM 10% and MEM 5% FBS supplementation. The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. Vero E6 and Vero CCL-81 proliferation in SFM-EGF media on day one to four did not show a significant difference compared to MEM 10% or MEM 5% serum supplementation media. However, the OD values of both Vero E6 and Vero CCL-81 cells given SFM-EGF media produced an average value below MEM 10% but higher than MEM 5% FBS supplementation. As such, this study proved that utilizing SFM-EGF could support the proliferation of Vero E6 and Vero CCL-81 cells.
Effect of Plectranthus scutellarioides (L.) Leaf Extract as Natural Antibacterial Against Staphylococcus aureus and Escherichia coli Isolated From Dairy Cattle with Subclinical Mastitis Shafina Hanum, Farah; Witaningrum, Adiana Mutamsari; Puspitasari, Yulianna
Journal of Basic Medical Veterinary Vol. 11 No. 2 (2022): Journal of Basic Medical Veterinary, December 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i2.39799

Abstract

Mastitis is one of the causes of low milk production in dairy cows, and can be caused by bacteria such as Escherichia coli and Staphylococcus aureus. Plectranthus scutellarioides (L.) leaf has antibacterial compounds such as flavonoids, eugenol, polyphenols, steroids, tannins, and so on. The type of this research is experimental laboratory research that aims to test the antibacterial activity of the P. scutellarioides (L.) leaf extract with ethanol pro analysis as the solvent against E. coli and S. aureus bacteria isolated directly from the sample of dairy cow's milk which CMT score is (+) 3, by measuring its inhibition ability indicated by the presence of clear zone using the agar-well diffusion method. The extract concentrations tested are 5%, 10%, 20% and 40%. The result of the antibacterial test of P. scutellarioides (L.) leaf extract with ethanol p.a. as the solvent showed antibacterial activity against E. coli and S. aureus bacteria isolated directly from cow's milk samples with subclinical mastitis at certain concentrations.
Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro Saraswati, Afif Tasya; Sugihartuti, Rahmi; Puspitasari, Yulianna; Witaningrum, Adiana Mutamsari; Rachmawati, Kadek; Maslachah, Lilik; Raharjo, Hartanto Mulyo; Hisyam, Mirza Atikah Madarina; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51106

Abstract

This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.
Profile of Antibiotic Residue and Antibiotic Resistance in Broiler Chicken Meat in Indonesia: Public Health Importance Bura, Maria Antonia Yersi Dua; Effendi, Mustofa Helmi; Puspitasari, Yulianna
JURNAL KAJIAN VETERINER Vol 12 No 1 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i1.15360

Abstract

Keamanan pangan merupakan isu global dan menjadi perhatian bersama. Di bidang peternakan, penyalahgunaan antibiotik dalam praktik kesehatan hewan berdampak pada bahaya residu dan resistensi antimikroba pada produk pangan asal hewan bagi kesehatan masyarakat. Penelitian ini mengkaji profil residu dan resistensi antibiotik pada daging ayam broiler di Indonesia. Kajian dilakukan melalui tinjauan pustaka terhadap artikel penelitian yang dipublikasikan di jurnal ilmiah nasional dan internasional, prosiding seminar, dan laporan penelitian. Hasil penelitian mengungkapkan, dalam lima tahun terakhir (2018-2023) masih ditemukan residu antibiotik pada daging ayam broiler di beberapa daerah di Indonesia. Pada periode yang sama, resistensi antibiotik juga ditemukan pada sampel daging ayam broiler di beberapa wilayah Indonesia. Resistensi antibiotik yang ditemukan adalah pada satu jenis antibiotik dan beberapa jenis antibiotik (multidrug resistance) sekaligus. Temuan ini dapat dijadikan bahan evaluasi perbaikan manajemen rantai produksi dan distribusi produk daging ayam broiler dalam negeri tentang jaminan keamanan pangan asal hewan.
Co-Authors Aan Nurfahrudianto Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Afandi, Zainal Agus Muji Santoso Aswin, Ahmad Bea Hana Siswati BUDI UTOMO Budiarto Budiarto Budiarto Bura, Maria Antonia Yersi Dua Dadik Rahardjo, Dadik Delaiah, Diena Diyantoro Diyantoro, Diyantoro Dua Bura, Maria Antonia Yersi Elis Irmayanti Endang Sri Mujiwati Endang Suprihati Ericka Darmawan Guruh Sukma Hanggara Hani Plumeriastuti Helen Susilowati, Helen Hermin Ratnani Heru Budiono Hidanah, Sri Hisyam, Mirza Atikah Madarina Jola Rahmahani Jola Rahmahani Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Khalissa Farah Alifia Kinasih, Kurnia Nisa Kirana, Sabila Citra Kuncorojakti, Suryo Kurniawan, Muhammad 'Ahdi Kusnoto Kusnoto, Kusnoto Larasati, Anissa Dhea Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Maulana, Firdausy Kurnia Mohamad Amin Moses, Ikechukwu Benjamin Mufasirin Mustika, Yolla Rona Mustofa Helmi Effendi Nidom, Chairul Anwar Permatasari, Dian Ayu Poedji Hastutiek Poppy Rahmatika Primandiri Pratama, Bima Putra Rahadyan, Raihan Alif Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmi Sugihartuti, Rahmi Ramadhani, Fifi Fauziah Rimayanti Rimayanti Riwu, Katty Hendriana Priscilia Rizhal Hendi Ristanto Rochmah Kurnijasanti Rodprasert, Watchareewan Rosyta, Pegy Sabdoningrum, Emy Koestanti Saraswati, Afif Tasya Setiawan Koesdarto Setyowidodo, Irwan Shafina Hanum, Farah Siti Aizah Sri Chusniati Suwarno Suwarno Suwarno Tacharina, Martia Rani Tita Damayanti Lestari Tri Julianto Tyasningsih, Wiwiek Widjiati Widjiati, Widjiati Wijaya, Andi Yasmin Wiwiek Tyasningsih Wiwik Misaco Yuniarti Yeni Dhamayanti