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INTRODUCTION STUDY OF MDR STAPHYLOCOCCUS AUREUS IN PIG SLAUGHTER HOUSE IN KUPANG CITY Sanam, Maxs U. E.; Kallau, Novalino H. G.; Sitompul, Yeremia Y.
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.7870

Abstract

Due to the unprudent use of antibiotics globally, it causes the incidence of antibiotic resistance with the emergence of bacteria that are resistant to antibiotics and has implications for the failure of the use of antibiotics in dealing with various cases of diseases caused by bacteria. The incidence of antibiotic resistance in the livestock sector occurs due to the use of antibiotics that are not based on recommendations and antibiotics are given in the long term. The presence of bacteria that carry resistance to one or several kinds of antibiotics in livestock is a threat to animal and human health. The city of Kupang as an area that has pig farms and a high pig population has a big challenge to the emergence of antibiotic resistance due to the use of antibiotics in maintaining livestock health. One of the bacteria that has a large number of distributions and is widely found in livestock and is an indicator of antibiotic resistance is Staphylococcus aureus (S. aureus). This research has used an observational research method based on data collection methods and laboratory examinations, which are adjusted to the objectives that have been set. The approach that has been used in this research is a Cross Sectional Study, because it wants to get an overview of S. aureus bacteria that are resistant to antibiotics. The sample that has been needed is pig feces obtained by field observations. The data analysis technique that has been used is a descriptive analysis model, to explain the presence of antibiotic-resistant S. aureus bacteria in pig farms. The results showed that this isolation and identification process resulted in 28 (51.85%) samples that were positive for S. aureus. The highest prevalence of resistance was found in S. aureus which was resistant to the antibiotic Colistin sulfate (82.1%). The number of antibiotics tested from S. aureus isolates showed various variations from 0 to 3 types of antibiotics that were resistant in 1 isolate. The most common resistance pattern shown in S. aureus isolates was the CS (Colistin sulfate) pattern as many as 19 of the 28 samples. The conclusion of this study encourages the need to increase supervision related to the use of antibiotics and increase public awareness of the use of antibiotics and prevention of the increasing incidence of antibiotic resistance.
Diagnosa Penyakit Hewan dengan Teknik Polymerase Chain Reaction di Lingkungan Universitas Nusa Cendana Gelolodo, Maria Aega; Pandarangga, Putri; Simarmata, Yohanes T. R. M. R.; Utami, Tri; Tophianong, Tarsisius Considus; Datta, Frans Umbu; Sitompul, Yeremia Y.; Gaina, Cynthia Dewi; Foeh, Nancy Diana F. K.; Deta, Herlina Umbu
International Journal of Community Service Learning Vol. 7 No. 2 (2023): May 2023
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v7i2.55140

Abstract

Penyakit infeksius dapat menyebabkan kerugian ekonomi dan dampak sosial budaya di masyarakat. Diagnosa dan deteksi dini terhadap agen patogen penyebab penyakit infeksius merupakan langkah penting dalam mencegah efek yang lebih besar dari penyakit infeksius. Semakin cepat suatu agen penyakit terdeteksi maka makin cepat pula usaha pencegahan maupun kontrol yang dilakukan untuk mencegah penyebaran lebih lanjut dari penyakit tersebut. Salah satu teknik deteksi laboratorik yang paling banyak digunakan saat ini adalah teknik molekuler PCR. Teknik ini memiliki sensitivitas dan spesifitas yang tinggi dan dapat dilaksanakan dalam waktu yang relatif singkat. Terlepas dari keunggulan serta keuntungan teknik PCR ini, namun pada kenyataannya masih ada stakeholders di tingkat universitas yang belum sepenuhnya mengenal maupun memahami dengan baik teknik ini. Oleh karena itulah maka pelatihan PCR pada para dosen maupun tenaga laboran di lingkungan Universitas Nusa Cendana, Kupang menjadi penting dilakukan dalam rangka peningkatan kapasitas deteksi laboratorik terhadap penyakit infeksius. Kegiatan yang diikuti oleh 14 orang peserta ini selain melengkapi para peserta dengan pengetahuan mengenai prinsip dasar PCR dan aplikasinya juga melatih kemampuan peserta dalam melaksanakan PCR di laboratorium.
Gambaran Hematologi 3 Bulan Pasca Vaksinisasi Porcine Reproductive and Respiratory Syndrome (PRRS) pada Ternak Babi di Kabupaten Kupang Simarmata, Yohanes T. R. M. R.; Gelolodo, Maria A.; Sitompul, Yeremia Y.; Sole, Marsyella G.
JURNAL KAJIAN VETERINER Vol 12 No 2 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i2.15132

Abstract

Pigs are the most commonly raised livestock in Kupang Regency, offering various advantages over other animals. However, the growing pig population poses a higher risk of diseases such PRRS, which affects both respiratory and reproductive health. Vaccination remains a crucial method for preventing PRRS. Post-vaccination haematological examinations are essential for assessing immune responses, utilizing parameters like leukocyte and lymphocyte counts to evaluate vaccine efficacy and safety. This study investigates haematological parameters as indicators of physiological responses to PRRS vaccination, a relatively less explored area compared to other immunological assessments. The research analysed haematological parameters such as RBC, HGB, PCV, MCV, MCH, MCHC, and WBC both before and three months after vaccination. Blood samples were collected from 15 pigs one day prior to and three months following vaccination. SPSS software was used to analyse the data. The results indicated that haematological parameters remained within normal ranges. RBC, HGB, PCV, and MCHC did not significantly differ, however MCH, MCV, and WBC levels did indicate statistically significant variations. These results support the safety and efficacy of PRRS immunization by indicating that it causes detectable haematological alterations. This study underscores the importance of using haematological parameters as reliable indicators for assessing vaccine pigs reaction.
Peningkatan Kapasitas Dokter Hewan Sebagai Garis Depan Penanganan Penyakit Rabies di Nusa Tenggara Timur Gelolodo, Maria Aega; R. M. R, Yohanes T.; Simarmata, Simarmata; Sitompul, Yeremia Y.; Kallau, Novalino H.G; Loe, Fhady R.
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 5 No. 3 (2024): Jurnal Pengabdian kepada Masyarakat Nusantara (JPkMN) Edisi Mei- Agustus
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v5i3.3468

Abstract

Dalam satu dekade belakangan ini kejadian penyakit akibat penyakit-penyakit zoonosois merupakan salah satu ancaman penting yang dihadapi oleh masyarakat dunia. Selain kemunculan emerging diseases dan re-emerging diseases, kejadian penyakit-penyakit zoonosis yang tergolong sebagai penyakit terabaikan (neglacted diseases) juga tetap dilaporkan dari berbagai wilayah di dunia dan menjadi ancaman kesehatan masyarakat penting. Rabies merupakan penyakit zoonosis yang paling tua namun sampai dengan saat ini masih menjadi ancaman serius bagi hewan dan manusia, terutama pada negara-negara seperti Indonesia. Sebagai penyakit penting yang berdampak bagi lingkungan manusia dan hewan maka partisipasi aktif dari berbagai elemen terkait kesehatan hewan maupun manusia merupakan kunci dalam pemberantasan penyakit ini. Dokter hewan sebagai garda terdepan penanganan penyakit asal hewan berperan penting dalam eradikasi penyakit ini. Kegiatan yang diikuti oleh 30 orang dokter hewan yang bertugas di berbagai wilayah di Provinsi Nusa Tenggara Timur ini bertujuan untuk meningkatkan kapasitas dokter hewan dalam investigasi penyakit seperti rabies. Kegiatan ini dilaksanakan dengan metode berupa presentasi dari pemateri utama, diskusi dan kerja kelompok serta praktik lapangan. Dari hasil pre-test, post-test serta feedback dari peserta diketahui bahwa terjadi peningkatan pengetahuan peserta akan investigasi penyakit dan rata-rata peserta puas akan kegiatan ini dan metode yang digunakan.  Kegiatan ini diharapkan dapat membekali para dokter hewan dengan kemampuan peneyelidikan penyakit yang handal sehingga peran serta dokter hewan dalam pengendalian maupun pemberantasan penyaki-penyakit zoonosis dapat semakin nyata terasa.
Spatial Analysis of the Occurrence of Multi-Drug Resistance (MDR) Escherichia coli in Pet Dogs in Kupang City Kallau, Novalino H.G.; Sanam, Maxs U.E.; Utami, Tri; Sitompul, Yeremia Y.
JURNAL INFO KESEHATAN Vol 21 No 2 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss2.1225

Abstract

Antibiotic resistance is an important health problem and a threat to public health. Dogs as pets can be both spreaders and reservoirs of antibiotic-resistant bacteria. Based on spatial analysis, this study aims to determine the distribution pattern of multi-drug resistance (MDR) Escherichia coli found in pet dogs in Kupang City. The spatial analysis method is based on the convex hull technique, elementary analysis of disease, and the Nearest Neighbour Index (NNI). Information on antibiotic-resistant E. coli has been obtained from the laboratory analysis results, and secondary data was obtained for spatial analysis. The results of this study have shown that the distribution pattern of the incidence of MDR E. coli in the City of Kupang in 2020 is a clustered pattern with the Nearest Neighbor Index (NNI) value of 0.783,  which is higher than the NNI AMR E. coli in the City of Kupang of 0.763. The incidence of MDR E. coli that has occurred in domesticated dogs takes place with a prevalence rate of 35% in an area of 5079 Ha. These results have shown that the incidence of MDR E. coli has spread to several areas in Kupang City and is a threat to public health. It requires appropriate prevention and control measures by implementing good hygiene and sanitation in the relationship between humans and their pets.