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All Journal International Journal of Community Service Learning JURNAL KAJIAN VETERINER
Sitompul, Yeremia Y.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Veterinary

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INTRODUCTION STUDY OF MDR STAPHYLOCOCCUS AUREUS IN PIG SLAUGHTER HOUSE IN KUPANG CITY Sanam, Maxs U. E.; Kallau, Novalino H. G.; Sitompul, Yeremia Y.
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.7870

Abstract

Due to the unprudent use of antibiotics globally, it causes the incidence of antibiotic resistance with the emergence of bacteria that are resistant to antibiotics and has implications for the failure of the use of antibiotics in dealing with various cases of diseases caused by bacteria. The incidence of antibiotic resistance in the livestock sector occurs due to the use of antibiotics that are not based on recommendations and antibiotics are given in the long term. The presence of bacteria that carry resistance to one or several kinds of antibiotics in livestock is a threat to animal and human health. The city of Kupang as an area that has pig farms and a high pig population has a big challenge to the emergence of antibiotic resistance due to the use of antibiotics in maintaining livestock health. One of the bacteria that has a large number of distributions and is widely found in livestock and is an indicator of antibiotic resistance is Staphylococcus aureus (S. aureus). This research has used an observational research method based on data collection methods and laboratory examinations, which are adjusted to the objectives that have been set. The approach that has been used in this research is a Cross Sectional Study, because it wants to get an overview of S. aureus bacteria that are resistant to antibiotics. The sample that has been needed is pig feces obtained by field observations. The data analysis technique that has been used is a descriptive analysis model, to explain the presence of antibiotic-resistant S. aureus bacteria in pig farms. The results showed that this isolation and identification process resulted in 28 (51.85%) samples that were positive for S. aureus. The highest prevalence of resistance was found in S. aureus which was resistant to the antibiotic Colistin sulfate (82.1%). The number of antibiotics tested from S. aureus isolates showed various variations from 0 to 3 types of antibiotics that were resistant in 1 isolate. The most common resistance pattern shown in S. aureus isolates was the CS (Colistin sulfate) pattern as many as 19 of the 28 samples. The conclusion of this study encourages the need to increase supervision related to the use of antibiotics and increase public awareness of the use of antibiotics and prevention of the increasing incidence of antibiotic resistance.
Diagnosa Penyakit Hewan dengan Teknik Polymerase Chain Reaction di Lingkungan Universitas Nusa Cendana Gelolodo, Maria Aega; Pandarangga, Putri; Simarmata, Yohanes T. R. M. R.; Utami, Tri; Tophianong, Tarsisius Considus; Datta, Frans Umbu; Sitompul, Yeremia Y.; Gaina, Cynthia Dewi; Foeh, Nancy Diana F. K.; Deta, Herlina Umbu
International Journal of Community Service Learning Vol. 7 No. 2 (2023): May 2023
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v7i2.55140

Abstract

Penyakit infeksius dapat menyebabkan kerugian ekonomi dan dampak sosial budaya di masyarakat. Diagnosa dan deteksi dini terhadap agen patogen penyebab penyakit infeksius merupakan langkah penting dalam mencegah efek yang lebih besar dari penyakit infeksius. Semakin cepat suatu agen penyakit terdeteksi maka makin cepat pula usaha pencegahan maupun kontrol yang dilakukan untuk mencegah penyebaran lebih lanjut dari penyakit tersebut. Salah satu teknik deteksi laboratorik yang paling banyak digunakan saat ini adalah teknik molekuler PCR. Teknik ini memiliki sensitivitas dan spesifitas yang tinggi dan dapat dilaksanakan dalam waktu yang relatif singkat. Terlepas dari keunggulan serta keuntungan teknik PCR ini, namun pada kenyataannya masih ada stakeholders di tingkat universitas yang belum sepenuhnya mengenal maupun memahami dengan baik teknik ini. Oleh karena itulah maka pelatihan PCR pada para dosen maupun tenaga laboran di lingkungan Universitas Nusa Cendana, Kupang menjadi penting dilakukan dalam rangka peningkatan kapasitas deteksi laboratorik terhadap penyakit infeksius. Kegiatan yang diikuti oleh 14 orang peserta ini selain melengkapi para peserta dengan pengetahuan mengenai prinsip dasar PCR dan aplikasinya juga melatih kemampuan peserta dalam melaksanakan PCR di laboratorium.
Gambaran Hematologi 3 Bulan Pasca Vaksinisasi Porcine Reproductive and Respiratory Syndrome (PRRS) pada Ternak Babi di Kabupaten Kupang Simarmata, Yohanes T. R. M. R.; Gelolodo, Maria A.; Sitompul, Yeremia Y.; Sole, Marsyella G.
JURNAL KAJIAN VETERINER Vol 12 No 2 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i2.15132

Abstract

Pigs are the most commonly raised livestock in Kupang Regency, offering various advantages over other animals. However, the growing pig population poses a higher risk of diseases such PRRS, which affects both respiratory and reproductive health. Vaccination remains a crucial method for preventing PRRS. Post-vaccination haematological examinations are essential for assessing immune responses, utilizing parameters like leukocyte and lymphocyte counts to evaluate vaccine efficacy and safety. This study investigates haematological parameters as indicators of physiological responses to PRRS vaccination, a relatively less explored area compared to other immunological assessments. The research analysed haematological parameters such as RBC, HGB, PCV, MCV, MCH, MCHC, and WBC both before and three months after vaccination. Blood samples were collected from 15 pigs one day prior to and three months following vaccination. SPSS software was used to analyse the data. The results indicated that haematological parameters remained within normal ranges. RBC, HGB, PCV, and MCHC did not significantly differ, however MCH, MCV, and WBC levels did indicate statistically significant variations. These results support the safety and efficacy of PRRS immunization by indicating that it causes detectable haematological alterations. This study underscores the importance of using haematological parameters as reliable indicators for assessing vaccine pigs reaction.
Co-Authors Deta, Herlina Umbu Foeh, Nancy Diana F. K. Gaina, Cynthia Dewi Kallau, Novalino Harold Geoffrey Maria Aega Gelolodo Maxs Urias Ebenheizer Sanam Simarmata, Yohanes T. R. M. R. Sole, Marsyella G. Tarsisius Considus Tophianong, Tarsisius Considus Tri Utami