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All Journal HAYATI Journal of Biosciences International Journal of Integrated Health Sciences HIGEIA (Journal of Public Health Research and Development) Jurnal Riset Kesehatan Masyarakat
Hitipeuw, Derico
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Health Professions Public Health

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Uji Efektivitas Larvasida Bacillus thuringiensis var. israelensis Terhadap Kematian Larva Aedes Hitipeuw, Derico; Martini, Martini; Hestiningsih, Retno; Udijono, Ari
Jurnal Riset Kesehatan Masyarakat Vol 2, No 4 (2022): Oktober 2022
Publisher : Fakultas Kesehatan Masyarakat, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jrkm.2022.16486

Abstract

Dengue Hemorrhagic Fever is an infectious disease that often causes outbreaks. In Indonesia, the CFR of DHF in 2021 reached 0.96%, this figure increased from the previous year, which was 0.69%. Various efforts have been made to prevent transmission of the dengue virus, for example the use of larvicides for the eradication of Aedes, as a vector for transmitting dengue. The type of research in the Bio Larvicide B. Thuringiensis test is a pure experiment with a posttest only control design. The study was conducted to determine the effect of giving B. thuringiensis on the mortality of A. aegypti larvae in conditions that almost resembled their natural habitat. The study population was all larvae of Aedes instar III from the colonies of the Salatiga B2P2VRP Laboratory. Sampling was done randomly/simple random, ie all third instar larvae had the opportunity to be used as research and control samples. The mortality of Aedes larvae from the intervention of VectoBac WG (Bio Larvicide with the active ingredient B. thuringiensis) from each concentration at 60 minutes of observation, namely the mortality of Aedes larvae in the control group was 0%, at a concentration of 0.001 g/L was 93%, at a concentration of 0.002 g/L as much as 98%, a concentration of 0.004 g/L, 0.006 g/L and 0.008 g/L as much as 100%. The results of this study can be used as a reference for the use of VectoBac WG products as natural larvicides and an alternative in controlling mosquito vector populations.
Efikasi Larvasida Bacillus thuringiensis var. israelensis Terhadap Larva Aedes, Anopheles, dan Culex Hitipeuw, Derico; Martini, Martini; Hestiningsih, Retno; Udijono, Ari; Yuliawati, Sri
HIGEIA (Journal of Public Health Research and Development) Vol 7 No 2 (2023): April 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/higeia.v7i2.66286

Abstract

Berdasarkan data Profil Kesehatan Indonesia pada tahun 2020, kasus DBD dilaporkan sebanyak 108.303 kasus, chikungunya 1.689 kasus, filariasis 9.906 kasus, sedangkan malaria dengan Annual Parasite Incidence sebesar 0,9%. Sejauh ini pengendalian vektor nyamuk menggunakan insektisida temephos sebagai larvasida yang dapat berdampak resistensi pada vektor serta kerusakan lingkungan, sehingga diperlukan pengendalian vektor nyamuk secara biologi. Penelitian ini digunakan untuk mengetahui efikasi atas Bio Larvasida dengan bahan aktif Bacillus thuringiensis terhadap kematian larva Aedes, Anopheles, dan Culex. Penelitian ini merupakan penelitian kuantitatif, menggunakan eksperimen murni dengan posttest only control design, yang dilaksanakan pada bulan April 2022. Terjadi kematian larva Aedes, Anopheles, dan Culex secara signifikan sejalan dengan peningkatan konsentrasi pada Bio Larvasida dengan bahan aktif B. thuringiensis dan terjadi perubahan secara morfologi terhadap larva yang telah terpapar Bio Larvasida dengan bahan aktif B. thuringiensis. Bio Larvasida dengan bahan aktif B. Thuringiensis dinilai efektif untuk digunakan sebagai alternatif larvasida alami dalam pengendalian populasi vektor nyamuk. Kata kunci: Efikasi, Bio Larvasida, B. thuringiensis
Detection of Leptospira sp. Bacteria and Factors Related to the Incidence of Leptospirosis in Semarang City Diyana, Sutra; Sutiningsih, Dwi; Adi, Mateus Sakundarno; Hitipeuw, Derico
International Journal of Integrated Health Sciences Vol 12, No 1 (2024)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/ijihs.v12.n1.3767

Abstract

Objective: The Case Fatality Rate (CFR) of Leptospirosis in Semarang City has shown a tendency to increase from 2019 to 2022. In 2022, there were 30 cases reported, with 8 deaths (CFR 27%). The purpose of this study was to detect Leptospira sp. bacteria in rats and analyze the risk factors for leptospirosis in Semarang City.Methods: This study employed an observational approach with a Case-Control Study design. The research was conducted in Semarang City from November to December 2023. Non-random sampling techniques were used, specifically the total sampling method, resulting in a sample size of 45 case groups and 45 control groups. Bivariate analysis was performed using the Chi-square test, with a significance level set at <0.05.Results: Based on the Chi-Square test results, the following variables were found to be associated with the incidence of leptospirosis in Semarang City: the presence of rats (p<0.0001), the presence of Leptospira sp. bacteria (p<0.0001), house conditions (p=0.260), sewer condition (p<0.0001), presence of pets (p=0.001), condition of landfills (p=0.001), presence of vegetation (p=0.005), temperature (p=0.299), humidity (p=0.495), lighting (p=0.023), history of wounds (p=0.001), and knowledge level (p=0.025).Conclusion: The risk factors for leptospirosis in Semarang City include the presence of rats, the presence of Leptospira sp. bacteria, sewer conditions, the presence of pets, conditions of landfills, the presence of vegetation, lighting, history of injury, and level of knowledge.
Molecular Characterization of Anisakis spp.: Potential Source of Fish-borne Zoonosis in Coastal Living Environment in Semarang, Indonesia Hitipeuw, Derico; Daud, Muhammad Muzhaffar; Nuranindita, Raisha; Diyana, Sutra; Amirudin, Fariha Akmalina; Rahayu, Aprillia Andika; Saputra, Edo Yungki; Qanita, Nabila Gusvie; Natanegara, Surya; Yuwanita, Mutiara Rizqi; Wusono, Ajeng Dwi; Martini, Martini; Muh, Fauzi
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.345-359

Abstract

Anisakis spp. are marine nematodes known to cause anisakiasis, a zoonotic disease transmitted through fish consumption with diverse clinical manifestations. In Indonesia, a country with extensive marine biodiversity and high fish consumption, data on anisakiasis remain limited. This study aimed to characterize the Anisakis spp. from the most significant commercial fish market in Semarang, Indonesia. A total of 17 Rastrelliger sp. were sampled. PCR amplification targeting the mitochondrial COX2 gene was used to identify the species of Anisakis spp. molecularly. Phylogenetic relationships, nucleotide diversity, and neutrality indices were assessed. The haplotype structures were visualized using the Haplotype Network. Subsequently, 7 of 17 (41.17%)  Rastrelliger sp. samples were infected with A. typica. Molecular analysis revealed two species, A. typica and H. amoyense, with high haplotype diversity (1.00±0.016, diversity±SD). Phylogenetic analysis revealed two major clades: A. typica (Semarang and Southern Makassar) and H. amoyense (Semarang, China, and Bangladesh), with both species indicating high genetic connectivity. Neutrality indices suggested purifying selection and population expansion for both species (dN-dS = -5.017). These findings highlight the genetic variability and zoonotic potential of Anisakis spp. in the commercial fish market, emphasizing the need for surveillance of fish-borne parasitic infections in Indonesia.
Co-Authors Amirudin, Fariha Akmalina Ari Udijono Daud, Muhammad Muzhaffar Diyana, Sutra Dwi Sutiningsih Martini Martini Mateus Sakundarno Adi, Mateus Sakundarno Muh, Fauzi Natanegara, Surya Nuranindita, Raisha Qanita, Nabila Gusvie Rahayu, Aprillia Andika Retno Hestiningsih Saputra, Edo Yungki Sri Yuliawati Wusono, Ajeng Dwi Yuwanita, Mutiara Rizqi