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All Journal International Journal of Public Health Science (IJPHS) The Indonesian Biomedical Journal
Rahmawati, Desty Restia
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Biochemistry, Genetics & Molecular Biology Health Professions Public Health

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Influence of croscarmellose in fast disintegrating tablet of Syzygium polyanthum extract Putranti, Widyasari; Rahmawati, Desty Restia; Sugihartini, Nining; Saifullah, Teuku Nanda
International Journal of Public Health Science (IJPHS) Vol 13, No 1: March 2024
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v13i1.22666

Abstract

Bay leaves (Syzygium polyanthum) contain the flavonoid quercetin which can be used as an antihyperlipidemic drug. The development of antihyperlipidemic drug formula in the form of fast disintegrating tablet (FDT) is needed for patients who experience dysphagia. FDT preparations require an optimal super disintegrant concentration to produce a good drug formula. This study aims to develop the FDT formula of bay leaves extract using the super disintegrant croscarmellose sodium (CCS) intra and extra-granular. FDT formulation using the wet granulation method with variations of CCS concentrations; F1: 2%, F2: 3.5%, and F3: 5% for extra granular, and 2% for intra granular. The formulation process, in-process control (IPC) granules, weight uniformity tests, and various physical properties tests of tablets were carried out. Data were statistically analyzed using one way ANOVA test (α=95%). The results of statistical tests of IPC granules, uniformity of weight, and tablet size of all FDT formulas were not significantly different (p>0.05). The CCS concentration for extra granular significantly affected the wetting time, disintegration time, hardness, and the value of friability percentage of FDT (p<0.05). The combination of intra and extra-granular CCS (2%:5%) gave the most optimum physical properties of bay leaf extract FDT.
Pentagamavunon-1 Enhances the Anticancer Effects of Doxorubicin on Triple-Negative Breast Cancer Cells in Monolayers and 3D Cancer Spheroid Models Rahmawati, Desty Restia; Murwanti, Retno; Jenie, Riris Istighfari; Nurrochmad, Arief
The Indonesian Biomedical Journal Vol 17, No 3 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i3.3587

Abstract

BACKGROUND: The 4T1 cells, a triple-negative breast cancer (TNBC) cell line, exhibit high malignancy and metastatic potential. As a primary treatment for TNBC, doxorubicin has limitations, including drug resistance mechanisms and severe side effects such as cardiotoxicity. Pentagamavunone-1 (PGV-1) exhibits antiproliferative and antimetastatic effects, induces prometaphase arrest, triggers cell senescence, and enhances reactive oxygen species (ROS) modulation, which may help overcome doxorubicin resistance. The selective cytotoxicity of PGV-1 against cancer cells suggests that it has a role in reducing systemic toxicity. Therefore, in this study, the anticancer effects of doxorubicin combined with PGV-1 was investigated.METHODS: Monolayer/2D and spheroid/3D models of 4T1 cells were used to assess the effects of PGV-1, doxorubicin, and their combination. MTT assay was used to evaluate the cytotoxicity, colony formation assay was used to measure persistent antiproliferative effects, and spheroid volume analysis was performed to assessed tumor growth inhibition. Senescence-associated beta-galactosidase (SA-β-gal) assay determined cellular senescence.RESULTS: The combination of PGV-1 and doxorubicin significantly enhanced cytotoxicity, with IC50 values of 0.57 µM and 4.88 µM, respectively (p=0.000). A strong synergistic effect was observed, leading to persistent suppression of cancer cell proliferation and an 80% reduction in colony formation (p=0.007). In the 3D spheroid model, combination treatment significantly reduced spheroid volume (p=0.002) more effectively than monotherapy, indicating superior growth inhibition and cytotoxicity. It also increased SA-β-gal, the senescence marker (p=0.010).CONCLUSION: The combination of PGV-1 and doxorubicin demonstrated potent anticancer effects in 4T1 monolayers and spheroid models by enhancing cytotoxicity and inducing cellular senescence. This combination confirmed its potential as a more effective therapeutic strategy.KEYWORDS: 3D spheroid, 4T1 TNBC cell, doxorubicin, pentagamavunon-1, PGV-1, senescence
Co-Authors Arief Nurrochmad Nining Sugihartini Retno Murwanti Riris Istighfari Jenie Teuku Nanda Saifullah, Teuku Nanda Widyasari Putranti