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AKTIVITAS ANTIBAKTERI DAUN PEPAYA (Carica Pepaya) MENGGUNAKAN PELARUT ETANOL TERHADAP BAKTERI Salmonella thypi Sudarwati, Tri Puji Lestari
Journal of Research and Technology Vol 4, No 1 (2018)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

Typhoid fever is one of the health problems that often occur in people of Indonesia. The spreads of this desease are through contaminated food. Thyphoid fever could be caused by bacterials infection, one of the bactery is Salmonella typhi. One of solution o cure the infection is by using antibacterial derived from plants or herbs called traditional medicine. This medicine is chosen and preffered because it has relatively small side effects. Carica papaya L. is the most kind of plant which is learnt for the function as antibacterial. This study aims to determine the concentration effects of papaya leaf extract to the inhibition zone of Salmonella typhi bacteria. The method to determine the concentration effect of papaya leaf extract is a paper disc difussion. The results of this study at a concentration of 20 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml and 100 μg/ml indicate inhibiton zone in the category of moderate.

LAJU PERKEMBANGAN Aedes aegypti PADA BEBERAPA MEDIA YANG BERBEDA Sudarwati, Tri Puji Lestari
Journal of Research and Technology Vol 1, No 01 (2015)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

This research was conducted to determine the pace of development of larvae of Aedes aegypti in some media of water, with the aim of knowing the development of Aedes aegypti mosquitoes from egg to be by looking at the development and physiology. The research was conducted between February and April 2008. The material used is the Aedes aegypti mosquito eggs laid on 3 media: water bath water, sewage, and water ponds. The data obtained were statistically analyzed using SPSS. The development of mosquito larvae in the sewer water is 8.3 days, 21.5 days of pool water, water bath of 24.3 days. Results of statistical analysis of all the observed variables showed that, there is a real difference between the sewage and the pool water and bath water, but the pool water and bath water are not significantly different. It can be concluded that the difference in water media affect the development of larvae of Aedes aegypti. Keywords: Aedes aegypti, water bath, water sewer, water ponds.

Concentration and Time Exposure Determination of Methanol Extract from Carica papaya Leaves in The Larvicidal Activity Against Aedes aegypti Larvae Sudarwati, Tri Puji Lestari; M. A Hanny Ferry Fernanda
Journal of Research and Technology Vol. 7 No. 1 (2021): JRT Volume 7 No 1 Jun 2021
Publisher : 2477 - 6165

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55732/jrt.v7i1.288

Dengue Haemorrhagic Fever (DHF) is a disease caused by dengue virus which is transmitted through Aedes aegypti mosquito bite. Efforts to control the widespread of the vectors have been made using biological agents and also chemical compound. Chemicals known as a standard protocol have raised concerns about resistance and harmfulness to the environment. Hence, the present study was aimed to explore the larvicidal activity of papaya (Carica papaya) leaf extract against Aedes aegypti larvae in regards to the optimum concentration and time exposure. Preparation the obtained extract was diluted to make a serial concentration. These solutions were made by pipetting 0.65 mL, 1.25 mL, 2.5 mL, 5.0 mL, and 10.0 mL of extract into 10.0 mL volumetric flasks and dilute with distilled water. The test solution was poured into a glass jar contained 90 mL of distilled water and filled with 20 third instar larvae. Each experiment was replicated four times. The larval mortality was recorded in 24h and calculated as a percentage of total larvae used in the experiment. The table above shows the value of LC50 And LT50 from toxicity assay of papaya leaf extract. According to the LT50 value, it can be seen that the lowest LT50 of 1,006h occurred at the concentration of 11000 ppm. Moreover, calculated LC50 is 4929,344 ppm. Based on these results, papaya leaves have the ability to Aedes aegypti larvaside so that it can help in breaking the chain of development of Aedes aegypti.

LAJU PERKEMBANGAN Aedes aegypti PADA BEBERAPA MEDIA YANG BERBEDA Sudarwati, Tri Puji Lestari
Journal of Research and Technology Vol. 1 No. 1 (2015): JRT Volume 1 No 1 Des 2015
Publisher : 2477 - 6165

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55732/jrt.v1i1.329

This research was conducted to determine the pace of development of larvae of Aedes aegypti in some media of water, with the aim of knowing the development of Aedes aegypti mosquitoes from egg to be by looking at the development and physiology. The research was conducted between February and April 2008. The material used is the Aedes aegypti mosquito eggs laid on 3 media: water bath water, sewage, and water ponds. The data obtained were statistically analyzed using SPSS. The development of mosquito larvae in the sewer water is 8.3 days, 21.5 days of pool water, water bath of 24.3 days. Results of statistical analysis of all the observed variables showed that, there is a real difference between the sewage and the pool water and bath water, but the pool water and bath water are not significantly different. It can be concluded that the difference in water media affect the development of larvae of Aedes aegypti.

Concentration and Time Exposure Determination of Methanol Extract from Carica papaya Leaves in The Larvicidal Activity Against Aedes aegypti Larvae Sudarwati, Tri Puji Lestari; M. A Hanny Ferry Fernanda
Journal of Research and Technology Vol. 7 No. 1 (2021): JRT Volume 7 No 1 Jun 2021
Publisher : 2477 - 6165

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55732/jrt.v7i1.288

Dengue Haemorrhagic Fever (DHF) is a disease caused by dengue virus which is transmitted through Aedes aegypti mosquito bite. Efforts to control the widespread of the vectors have been made using biological agents and also chemical compound. Chemicals known as a standard protocol have raised concerns about resistance and harmfulness to the environment. Hence, the present study was aimed to explore the larvicidal activity of papaya (Carica papaya) leaf extract against Aedes aegypti larvae in regards to the optimum concentration and time exposure. Preparation the obtained extract was diluted to make a serial concentration. These solutions were made by pipetting 0.65 mL, 1.25 mL, 2.5 mL, 5.0 mL, and 10.0 mL of extract into 10.0 mL volumetric flasks and dilute with distilled water. The test solution was poured into a glass jar contained 90 mL of distilled water and filled with 20 third instar larvae. Each experiment was replicated four times. The larval mortality was recorded in 24h and calculated as a percentage of total larvae used in the experiment. The table above shows the value of LC50 And LT50 from toxicity assay of papaya leaf extract. According to the LT50 value, it can be seen that the lowest LT50 of 1,006h occurred at the concentration of 11000 ppm. Moreover, calculated LC50 is 4929,344 ppm. Based on these results, papaya leaves have the ability to Aedes aegypti larvaside so that it can help in breaking the chain of development of Aedes aegypti.

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