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All Journal Indonesian Journal of Applied Research (IJAR) J.Pelastek Journal of Biotechnology and Natural Science Filogeni: Jurnal Mahasiswa Biologi Jurnal Abdimas

Astuti, Sutan Nur Chamida Tri

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Author-ID : 7598652

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Electrical & Electronics Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Nursing Social Sciences

Published : 8 Documents Claim Missing Document

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Articles

Title

The Potential of Salvinia molesta as a Copper Phytoremediation Agent based on Gene Expression Analysis Astuti, Sutan Nur Chamida Tri; Solihah, Jumailatus; Aisah, Siti
Journal of Biotechnology and Natural Science Vol. 3 No. 1 (2023): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i1.9739

This research explores the rapid environmental impact of the batik industry, particularly concerning batik waste pollution, especially from the heavy metal copper (Cu). Untreated batik waste can have adverse effects on the environment. The study highlights the potential of the water fern plant (Salvinia molesta) as a heavy metal phytoremediator, specifically for Cu, by activating phytochelatin synthase. The research aims to determine the most effective waste concentration for Cu absorption by water ferns, measure the reduction in Cu levels after phytoremediation treatment, evaluate post-phytoremediation water quality, and observe PCS gene expression in the roots and leaves of water ferns. A static method is employed with variations in waste concentrations. Data analysis utilizes one-way ANOVA for Cu level reduction and changes in water quality. The results indicate that the most effective wastewater concentration for absorbing heavy metal copper (Cu) using water fern (Salvinia molesta) is 2%. The highest reduction in heavy metal copper (Cu) concentration after phytoremediation treatment with water fern (Salvinia molesta) is 41.48%. Water quality post-phytoremediation treatment using water fern (Salvinia molesta) at all concentrations exhibits improvement with an increase in dissolved oxygen (DO) levels. The expression of the phytochelatin synthase (PCS) gene in the leaves and roots of water fern (Salvinia molesta) confirms the role of the PCS gene in binding heavy metal copper (Cu) to the plant's vacuole.

Inventory of Terrestrial Orchids in the Baturraden Botanical Garden of Central Java Astuti, Sutan Nur Chamida Tri; Aisah, Siti; Kurniawan, Ardyan Pramudya
Journal of Biotechnology and Natural Science Vol. 2 No. 1 (2022): June
Publisher : Universitas Ahmad Dahlan

Baturraden Botanical Garden located on the slopes of Mount Slamet in the south has a high enough humidity so it is ideal for plant growth, especially orchids. As an ex-situ plant conservation area, the Baturraden Botanical Garden plays an important role in preserving the diversity of orchids in it. The purpose of this study was to find out the types and descriptions of terrestrial orchids in the Baturraden Botanical Garden area of Central Java. The method used in this study is a 400 m line transect method with an inter-point distance of 100 m each and data collection is carried out at 10 m each on the right and left of the line transect. Hasil research shows that there are 13 species derived from 10 genus of soil orchids. The types that have been found are Goodyera rubicunda (Blume) Lindl., Calanthe pulchra (Blume) Lindl., Spathoglottis plicata Blume, Cryptostylis javanica J.J.Sm., Appendicula alba Blume, Phaius callosus (Blume) Lindl., Lepidogyne longifolia Blume, Tainia elongata J.J.Sm., Goodyera colorata Blume, Malaxis sp. Soland. ex Sw., Goodyera sp. R. Br., Goodyera procera (Ker-Gawl.) Hook., and Plocoglottis sp. Blume.

Toxicity of Copper (Cu) and Chromium (Cr) on the Seed Germination of Mung Bean (Vigna radiata L.) Astuti, Sutan Nur Chamida Tri; Nugroho, Andhika Puspito
Journal of Biotechnology and Natural Science Vol. 2 No. 2 (2022): December
Publisher : Universitas Ahmad Dahlan

Rapid industrial growth may have implications for an increase in cases of pollution of soil ecosystems through untreated waste disposal. Copper and chromium are metals found in some types of industrial wastewater, such as electroplating and silversmithing, that are essential at low concentrations but toxic at high concentrations. Vigna radiata was chosen as the test organism because it has been reported that the yield and production of the crop have decreased worldwide due to heavy metal pollution. This study aims to evaluate individual and mixed Cu and Cr toxicity in the seed germination of V. radiata. The individual and mixed Cu and Cr toxicity test was started with a range-finding and definitive test for 96 h. The toxicity level of the individual metal was expressed as IC50-96 h by probit analysis. For the mixed test, the organism was exposed to concentration ratios of Cu and Cr: 10%:90%; 35%:65%; 50%:50%; 65%:35%; and 90%:10% of individual IC50-96 h, each conducted for 96 h. The results showed that the individual IC50-96 h of Cu was 127.4 mg/L, while for Cr, it was 615.23 mg/L, indicating that Cu is more toxic than Cr. The mixed test showed that the IC50-96 h of Cu was 247.5 mg/L, 579.85 mg/L for Cr. The highest inhibition value at the mixed test was at a Cu and Cr concentration ratio of 90%:10%. Based on the mixed test, the interaction of Cu and Cr resulted in an additive effect (CI=1).

Antibiotic Resistance in Escherichia coli Isolated from Chicken Meat in Traditional Markets of Yogyakarta Al Farisi, Irsyad; Astuti, Sutan Nur Chamida Tri; Falih, Afifah Nurul; Mardiah, Irma; Aji, Oktira Roka
Journal of Biotechnology and Natural Science Vol. 5 No. 1 (2025): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v5i1.13125

The widespread use of antibiotics in the livestock industry serves both as a preventive measure against diseases and as a feed additive. However, excessive antibiotic use has led to antibiotic resistance, where bacterial exposure to antibiotics renders them resistant to one or more antibiotic agents. This study investigates the presence of antibiotic resistance in Escherichia coli isolated from chicken meat sold in traditional markets in Yogyakarta. An experimental method was employed, using ECB and EMBA media for bacterial isolation. Gram staining was performed to characterize the morphology of E. coli, followed by molecular testing using the uspA gene. Antibiotic susceptibility testing was conducted using the Kirby-Bauer disk diffusion method. A total of 10 chicken meat samples were collected from five traditional markets in Yogyakarta. The antibiotics tested included ampicillin, ciprofloxacin, and chloramphenicol. The results indicated resistance in all 10 isolates. Sample codes G1 and G2 were resistant to all three antibiotics. Samples P1, P2, and L1 exhibited resistance to ampicillin and chloramphenicol, with intermediate resistance to ciprofloxacin. Sample L2 showed resistance to ampicillin, intermediate resistance to ciprofloxacin, and sensitivity to chloramphenicol. Sample B1 exhibited resistance to all antibiotics tested. Sample B2 was resistant to ampicillin and ciprofloxacin, with intermediate resistance to chloramphenicol. Samples K1 and K2 were resistant to ampicillin and chloramphenicol but sensitive to ciprofloxacin. These findings highlight the growing concern of antibiotic resistance in bacterial strains isolated from chicken meat, emphasizing the need for stricter regulations on antibiotic use in the poultry industry.

Co-Authors Al Farisi, Irsyad Andhika Puspito Nugroho Falih, Afifah Nurul Ilma, Nur Malika Irsalinda, Nursyiva Kurniawan, Ardyan Pramudya Mardiah, Irma Mubarokah, Alifah Oktira Roka Aji Pratiwi, Ambar Putri, Diah Asta Siti Aisah Solihah, Jumailatus Suwartiningsih, Nurul Wasono, Mardi Wulandari, Endah Retno Yuliani, Ririn Kholifatu

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