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All Journal Global Medical and Health Communication Medika Kartika : Jurnal Kedokteran dan Kesehatan
Watanabe, Kozo
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Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Arbovirus Detection of Adult Female Aedes aegypti for Dengue Surveillance: a Cohort Study in Bandung City, Indonesia Faridah, Lia; Ekawardhani, Savira; Fauziah, Nisa; Djati, Imam Damar; Putra, Ramadhani Eka; Watanabe, Kozo
Global Medical & Health Communication (GMHC) Vol 11, No 3 (2023)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v11i3.12749

Abstract

Dengue surveillance is an important activity to prevent dengue outbreaks. This activity becomes a significant challenge for the region with limited logistic capabilities. Developing a simple mathematical model to predict the possibility of dengue incidence provides a reliable early warning system. This study compared the correlation between vector (adult female Aedes aegypti) and arbovirus detection on a vector to dengue incidence, which generalized linear mixed models tested. The incidence of adult female Aedes aegypti and dengue fever cases were interpolated through third-power inverse distance weighting (IDW). A spatial correlation between female Aedes aegypti incidence and dengue incidence was obtained from polynomial regression. Collection sites were 16 villages in Bandung city, one of the significant dengue endemic areas in January–December 2017. A total of 8,402 mosquitoes of Aedes aegypti, Aedes albopictus, and Culex sp., with 17% belonging to Aedes aegypti as the subject of the dengue virus (DENV) infection test. Data analysis only showed a weak correlation between the numbers of adult female Aedes aegypti and dengue incidence. On the other hand, there is no correlation between positive dengue infection of vector and dengue incidence. This study highlights the importance of constant arbovirus surveillance and integrated surveillance methods on all possible dengue vectors to develop an early warning system for dengue incidence.
Promotion of Crypt-like Structures in Intestinal Organoid Development through the Addition of Graphene Oxide in Cell-based Assays Sulaksono, Haura Labibah Salsabil; Kamilah, Mutiara Mila; Faridah, Lia; Joni, I Made; Watanabe, Kozo; Ekawardhani, Savira
Global Medical & Health Communication (GMHC) Vol 12, No 3 (2024)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v12i3.13386

Abstract

The intestinal organoid represents a miniature organ that can mimic functional physiology and pathology. However, there are several challenges to developing the organoid system, such as the limited survival of cells. Based on theory, matrix addition is a factor that can support survival in cells. As a result, graphene oxide (GO) addition is used in this study. As an artificial matrix, GO has been successfully shown to encourage good cell behavior and is well known for having good biocompatibility. Herein, we fabricate GO characterized with FT-IR and PSA. Crypt-like structures (CLS) are isolated from small intestinal mice in GO addition as a matrix. The gene expression and cell viability of CLS are investigated. RT-PCR examined the gene expression in CLS, while cell viability of CLS was carried out using the staining method. This study was conducted at FiNder U-CoE and Parasitology Laboratory of HSE Universitas Padjadjaran Bandung during February and December 2023. Our results show that Vil-1 as an identity for cells in the intestinal epithelium has been expressed in CLS primary significantly higher than intestinal tissue (p=0.01). However, identifying Lgr5 in CSL isolates is tricky. Thes in the crypt may be limited. Besides that, cell viability of CLS with GO addition can be maintained for four days. The GO addition as a matrix may provide support to maintain CLS. These findings are promising as cell-based assays for developing organoid models.
BIODIVERSITY OF ADULT FEMALE MOSQUITOES: ACTIVE SURVEILLANCE TO ADDRESS MOSQUITO-BORNE DISEASES IN CIMAHI, INDONESIA Faridah, Lia; Thufail, Muhammad Akbar; Zahra, Ridha Beta; Fauziah, Nisa; Qaromah, Annisa Abdiwijaya; Watanabe, Kozo
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 9 No 1 (2026): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Abstract

Mosquito-borne diseases are a global health threat, especially in developing cities such as Cimahi City, West Java, which has shown an increase in cases of dengue fever and filariasis. Limited data on adult mosquito vectors in Cimahi prompted this exploratory descriptive study. A total of 439 female mosquitoes were collected from 15 villages during the dry and rainy seasons using light traps, then identified morphologically. Diversity analysis was conducted using the Shannon-Wiener index (H'), while comparisons between locations were performed using the Kruskal-Wallis test due to non-normal data distribution. The results showed that Culex spp. (54.67%) dominated, followed by Aedes aegypti (32.19%), Armigeres spp. (10.02%), and Aedes albopictus (3.19%). Baros had the highest number of mosquitoes (77), while Central Cimahi had the lowest (11). The majority of villages showed low diversity (H' ≤ 1) and uneven distribution (J < 0.5) with the dominance of certain species. The Kruskal-Wallis test found no significant difference in the number of mosquitoes between villages (p = 0.993). Keywords: Cimahi City, mosquito diversity, mosquito-borne diseases DOI : 10.35990/mk.v9n1.p68-78
Co-Authors Djati, Imam Damar Fauziah, Nisa I Made Joni Kamilah, Mutiara Mila Lia Faridah Qaromah, Annisa Abdiwijaya Ramadhani Eka Putra Savira Ekawardhani Sulaksono, Haura Labibah Salsabil Thufail, Muhammad Akbar Zahra, Ridha Beta