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All Journal Global Medical and Health Communication Molecular and Cellular Biomedical Sciences (MCBS)
Kamilah, Mutiara Mila
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Cytotoxicity Effect of Aqueous Propolis Extract of Geniotrigona thoracica Sumatrans on Colo-201 Colon Cancer Cell Line and Senescence Colo-201 Colon Cancer Cell Line Induced by Low-dose Doxorubicin Latama, Zahra Nabila; Achadiyani, Achadiyani; Kamilah, Mutiara Mila; Putra, Ramadhani Eka; Kinasih, Ida; Faridah, Lia; Ekawardhani, Savira
Global Medical & Health Communication (GMHC) Vol 12, No 2 (2024)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v12i2.13760

Abstract

Propolis, a resinous compound honeybees produce, demonstrates an extensive spectrum of powerful biological properties. However, the anti-cancer activity of propolis derived from Geniotrigona thoracica Sumatrans has yet to be reported. Thus, we sought to investigate the cytotoxicity of aqueous propolis extracts from Geniotrigona thoracica Sumatrans against Colo-201 colon cancer cell line and senescence Colo-201 colon cancer cell line induced by low-dose doxorubicin. This study was conducted at the Parasitology Laboratory of Human, Safety, and Environment, Universitas Padjadjaran Bandung from January to May 2024. This study assessed cell viability using the WST-1 test. Non-induced Colo-201 cells were treated with an aqueous extract of propolis (AEP) 100 ppm, or 5-fluorouracil (5-FU) 5 mg/ml as the positive control or water as a vehicle on untreated control. Colo-201 senescence was induced by doxorubicin 0.1 µM for three days. Doxorubicin-induced Colo-201 senescence was then treated with AEP 100 ppm, with 5-FU 5 mg/ml as the positive control, or with the combination of AEP 100 ppm and 5-FU 5 mg/ml, or water as a vehicle on untreated control. The data were analyzed using SPSS version 25.0, a one-way ANOVA, and Tukey’s post hoc test. The results showed that AEP has cancer-killing effects on Colo-201 cells and Colo-201 senescent cells induced by low-dose doxorubicin. AEP-treated Colo-201 cells and Colo-201 senescent cells induced by low-dose doxorubicin viability were significantly reduced to 37.15% and 13.72%, respectively, although slightly higher than those of the 5-FU-treated one at this concentration. There was also a decrease in the cancer-killing effect of 5-FU from 88.55% in non-induced Colo-201 cells to 41.5% in the doxorubicin-induced Colo-201 senescence model. In conclusion, aqueous extract of propolis from Geniotrigona thoracica Sumatrans showed cancer-killing-effects both on the Colo-201 colon cancer cell line and senescence Colo-201 colon cancer cell line induced by low-dose doxorubicin.
Promotion of Crypt-like Structures in Intestinal Organoid Development through the Addition of Graphene Oxide in Cell-based Assays Sulaksono, Haura Labibah Salsabil; Kamilah, Mutiara Mila; Faridah, Lia; Joni, I Made; Watanabe, Kozo; Ekawardhani, Savira
Global Medical & Health Communication (GMHC) Vol 12, No 3 (2024)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v12i3.13386

Abstract

The intestinal organoid represents a miniature organ that can mimic functional physiology and pathology. However, there are several challenges to developing the organoid system, such as the limited survival of cells. Based on theory, matrix addition is a factor that can support survival in cells. As a result, graphene oxide (GO) addition is used in this study. As an artificial matrix, GO has been successfully shown to encourage good cell behavior and is well known for having good biocompatibility. Herein, we fabricate GO characterized with FT-IR and PSA. Crypt-like structures (CLS) are isolated from small intestinal mice in GO addition as a matrix. The gene expression and cell viability of CLS are investigated. RT-PCR examined the gene expression in CLS, while cell viability of CLS was carried out using the staining method. This study was conducted at FiNder U-CoE and Parasitology Laboratory of HSE Universitas Padjadjaran Bandung during February and December 2023. Our results show that Vil-1 as an identity for cells in the intestinal epithelium has been expressed in CLS primary significantly higher than intestinal tissue (p=0.01). However, identifying Lgr5 in CSL isolates is tricky. Thes in the crypt may be limited. Besides that, cell viability of CLS with GO addition can be maintained for four days. The GO addition as a matrix may provide support to maintain CLS. These findings are promising as cell-based assays for developing organoid models.
The μDrop Method Enhances Melanin Content Measurement in the in vitro Melanogenesis Model Using B16F10 Cell Line Yuliarni, Dinda; Kamilah, Mutiara Mila; Nugraha, Gaga Irawan; Faridah, Lia; Bashari, Muhammad Hasan; Ekawardhani, Savira
Molecular and Cellular Biomedical Sciences Vol 9, No 1 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i1.527

Abstract

Background: The B16F10 cell line is a cell frequently used in melanin content assays. However, reports on cell models using B16F10 are limited, particularly as the robust model cell in the Indonesian cosmetics industry. We found measuring melanin content using microplate spectrophotometry to be challenging, so this research was conducted to develop a method using μDrop spectrophotometry.Materials and methods: In this in vitro study, the B16F10 melanoma cell line was cultured in Roswell Park Memorial Institute (RPMI) medium containing 5% fetal bovine serum (FBS). The cells were categorized into control, stimulated, and treated groups. Melanogenesis stimulation was achieved using 1μM α-melanocyte-stimulating hormone (α-MSH), while inhibition using 800 μg/ml kojic acid. After treatment, the cells were incubated for 48 hours. Their melanin content was then measured using an ELISA reader with a μDrop method and compared with the microplate method. Statistical analysis used a one-way ANOVA test with Turkey’s Post Hoc analysis.Results: The μDrop method increased the melanin signal into the linear range of machine readings, while the signals from the microplate method fell far below this range. The B16F10 melanoma cell lines stimulated by α-MSH exhibited increased melanin production compared with the control group, while kojic acid treatment significantly reduced (p<0.05) melanin content in the stimulated group.Conclusion: The μDrop method significantly outperformed the microplate method in measuring melanin content within melanogenesis cell models, offering enhanced accuracy and particularly excelling at quantifying low content of melanin. Keywords: μDrop, microplate, melanin, melanogenesis, B16F10 cell line, RPMI
Co-Authors Achadiyani Bashari, Muhammad Hasan Gaga Irawan Nugraha I Made Joni Ida Kinasih Latama, Zahra Nabila Lia Faridah Ramadhani Eka Putra Savira Ekawardhani Sulaksono, Haura Labibah Salsabil Watanabe, Kozo Yuliarni, Dinda