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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Penelitian Pendidikan IPA (JPPIPA) Seminar Nasional Lahan Suboptimal
Sidik, Effi Alfiani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Materials Science & Nanotechnology Physics Other

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Journal : Seminar Nasional Lahan Suboptimal

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Asosiasi Begomovirus dan Betasatelit dalam Pengendalian Penyakit Kuning melalui Pendekatan Bioteknologi Sidik, Effi Alfiani; Laeshita, Putri
Seminar Nasional Lahan Suboptimal Vol 11, No 1 (2023): Prosiding Seminar Nasional Lahan Suboptimal ke-11 “Optimalisasi Pengelolaan Lah
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Sidik, E.A., Laeshita, P.  (2023). Association of begomoviruses and betasatellit in controlling yellow disease through a biotechnological approach. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-11 Tahun 2023, Palembang 21 Oktober 2023. (pp. 660-672).  Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Begomovirus belongs to the Geminivirus family, which is the largest genus that causes disease in many plants. Begomovirus genomes are monopartite and bipartite. In recent years it has been known that monopartite and bipartite begomoviruses associate with ssDNA satellit (betasatellit). Recombination that occurs between begomoviruses and betasatellit can trigger the emergence of new species and play a role in spread. The goal of this article is to describe the association between begomoviruses and betasatellit and their role in control using biotechnological approaches. This article was studied applying a scientific literature review process. This association plays a role in triggering the emergence of symptoms in the pathogenicity process. The betasatellit region contains a single open reading frame (ORFs) (βC1 gene) as a determinant of pathogenicity. Begomoviruses and betasatellit contain ORFs that encode replication, transcription, and RNA silencing (suppressing gene silencing) activities. The existence of this association can be an alternative control that utilizes the application of RNA interference (RNAi) to control begomovirus infection. Implemented by producing transgenic plants that harbor two different RNAi hairpin intron constructs using conserved regions from the viral genome and ORFs βC1 from the betasatellit genome. The multifunctional nature of the encoded protein may be a promising alternative in the development of resistance against begomovirus infection. In the end, transgenic plants were produced with milder symptoms and less viral DNA accumulation.
Studi Molekuler Rice tungro bacilliform virus pada Ratun Padi di Sidrap, Sulawesi Selatan melalui Pendekatan PCR Sidik, Effi Alfiani; Ibrahim, E.; Firmansyah, F.; Khaerana, K.
Seminar Nasional Lahan Suboptimal Vol 12, No 1 (2024): Vol 12, No 1 (2024): Prosiding Seminar Nasional Lahan Suboptimal ke-12 “Revital
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Sidik, E.A., Ibrahim, E., Firmansyah, F. & Khaerana, K. (2024). Molecular study of rice tungro bacilliform virus on rice ratoons in Sidrap, South Sulawesi through a PCR Approach. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-12 Tahun 2024, Palembang  21 Oktober 2024. (pp. 836–842).  Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Rice rungro bacilliform virus (RTBV) is a primary causes of tungro disease, which proses a significant threat to rice production in South Sulawesi. This study was conducted to identify the infection symptoms and presence of RTBV in rice retoon in Sidrap, South Sulawesi. Samples were collected using purposive sampling in area suspected of tungro virus infection, and symptom identification was performed. RTBV detection was carried out using Polymerase Chain Reaction (PCR). Symptoms observed included yellow to orange discoloration of leaves, leaf shortening, and narrowing of leaf blades. PCR results confirmed that the ratoon rice was infected by RTBV. These finding indicated ratoons may serve as a potential inoculum source for virus spread in the mext planting season, thus prolonging the infection cycle. Early detection of RTBV infection in the ratooning phase through molecular approaches is crucial to minimizing the risk of tungro disease spread.
Co-Authors Ema Komalasari, Ema Firmansyah, F. Haq, Muhammad Syahidul Ibrahim, E. Ibrahim, Elisurya Ismayanti, Rini Judijanto, Loso Kadir, Dalimawaty Khaerana, K. Putri Laeshita, Putri Santosa, Tomi Apra Widodo , Joko