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All Journal Judika (Jurnal Nusantara Medika) Jurnal Sintesis: Penelitian Sains, Terapan dan Analisisnya Biocaster : Jurnal Kajian Biologi JENGGALA: Jurnal Riset Pengembangan dan Pelayanan Kesehatan Ficco Public Health Journal
Aulia Risqi Fatmariza
Institut Ilmu Kesehatan Bhakti Wiyata Kediri
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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Stabilitas Pooled Sera Etilen Glikol dan Pooled Plasma NaF Sebagai Bahan Kontrol Pemeriksaan Glukosa Fatmariza, Aulia Risqi; Kurniawan, Angga Eka; Sari, Nofita
Jenggala : Jurnal Riset Pengembangan dan Pelayanan Kesehatan Vol 3 No 2 (2024): DESEMBER 2024
Publisher : Fakultas Teknologi dan Manajemen Kesehatan

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Abstract

Analisis Korelasi Neutrophil-Lymphocyte Ratio (NLR) dengan Indikator Komplikasi Makrovaskular dan Mikrovaskular pada Diabetes Melitus Tipe 2 Hasanah, Fathul Hidayatul; Wahyuni, Sri; Primartati, Danny Meganingdyah; Fatmariza, Aulia Risqi; Sari, Fita
Biocaster : Jurnal Kajian Biologi Vol. 5 No. 3 (2025): July
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v5i3.501

Abstract

Type 2 Diabetes Mellitus (DM type 2) is associated with low-grade systemic inflammation. Neutrophil-Lymphocyte Ratio (NLR) is a pro-inflammatory and oxidative stress biomarker in cardiometabolic diseases such as T2DM. This study aims to analyze the relationship of NLR with leukocyte count, random blood glucose, creatinine, total cholesterol, Serum Glutamic Pyruvic Transaminase (SGPT), and Serum Glutamic Oxaloacetic Transaminase (SGOT). The study design is a retrospective analysis with respondents of 162 uncomplicated T2DM patients treated at Mojowarno Christian General Hospital, Jombang. The results of the study showed that the average number of leukocytes was 12,688 cells/µl, neutrophils 75.3%, lymphocytes 17.2%, NLR 8.5, blood glucose 273.2 mg/dl, SGOT 40.88 U/l, SGPT 54.64 U/l, total cholesterol 196.3 mg/dl (<200), and creatinine 1.13 mg/dl. Correlation tests showed that NLR was significantly associated with leukocyte count (p = 0.000 < 0.05) and total cholesterol (p = 0.000 < 0.05), but not significantly correlated with random blood glucose (p = 0.064 > 0.05), SGOT (p = 0.089 > 0.05), SGPT (p = 0.929 > 0.05), or creatinine (p = 0.625 > 0.05). In conclusion, in patients with type 2 diabetes, NLR was significantly correlated with leukocyte count and total cholesterol, but not with random blood glucose, SGOT, SGPT, or creatinine.
Pengaruh Siklus Freeze-thaw Terhadap Akurasi dan Presisi Pooled sera Glukosa Fatmariza, Aulia Risqi; Primartati, Danny Meganingdyah; Danny Meganingdyah Primartati
Judika (Jurnal Nusantara Medika) Vol 9 No 2 (2025): Judika Volume 9 No. 2 Oktober 2025
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/judika.v9i2.27073

Abstract

Siklus pembekuan dan pencairan (freeze–thaw) berulang sering kali tidak dapat dihindari dalam pengelolaan bahan kontrol laboratorium, terutama pada laboratorium dengan keterbatasan sumber daya. Glukosa merupakan salah satu parameter kimia klinik yang banyak diperiksa, sehingga penting untuk mengevaluasi stabilitasnya terhadap proses freeze–thaw. Tujuan: Penelitian ini bertujuan untuk menilai pengaruh siklus freeze–thaw terhadap akurasi dan presisi pooled sera glukosa. Metode: Penelitian eksperimental dilakukan menggunakan pooled sera dengan kadar glukosa tinggi yang dibagi menjadi aliquot dan melalui sepuluh kali siklus freeze–thaw. Pemeriksaan kadar glukosa dilakukan secara duplo pada setiap siklus. Analisis dilakukan dengan menghitung bias (%) terhadap nilai target awal, koefisien variasi (CV%), serta distribusi data (mean, median, minimum, maksimum). Hasil: Kadar glukosa rata-rata menunjukkan rentang 231,5–236 mg/dL dengan nilai mean 233,75 mg/dL, median 233,5 mg/dL, minimum 231,5 mg/dL, dan maksimum 236 mg/dL. Bias maksimum yang diperoleh adalah -1,91% dan nilai CV% ≤0,30%, yang keduanya masih memenuhi standar akurasi dan presisi berdasarkan ISO 15189 dan CLSI EP15-A3. Kesimpulan: Pooled sera glukosa terbukti stabil terhadap sepuluh kali siklus freeze–thaw tanpa penurunan signifikan pada akurasi maupun presisi, sehingga berpotensi digunakan sebagai alternatif bahan kontrol internal level tinggi yang efektif dan efisien di laboratorium klinik.
Hubungan Upaya Pemberantasan Sarang Nyamuk dengan Kejadian Demam Berdarah Dengue di Indonesia Tahun 2023 Sari, Nofita; Fatmariza, Aulia Risqi
Ficco Public Health Journal Vol. 1 No. 03 (2024): December 2024
Publisher : Ficco Scientific Corner

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.14949247

Abstract

Background: This study aims to identify the relationship between mosquito nest eradication efforts and the prevalence of dengue fever in Indonesia. Methods: The study used a quantitative method with a descriptive analytic design and a cross-sectional approach, where the independent variables (sowing larvicide powder, installing mosquito screens on vents, draining bathtubs, closing water reservoirs, and destroying used items) were analysed against the dependent variable, which was the prevalence of DHF based on the Indonesian Health Survey data in 2023. Data were analysed univariately and bivariately with Spearman correlation test to see the relationship between variables. Results: The results showed that installation of mosquito screens on house ventilation and destruction of used goods had a significant association with the prevalence of DHF (p=0.006 and p=0.042, respectively), while sowing larvicidal powder, draining bathtubs, and closing water reservoirs did not show a significant association. Conclusions: This study concludes that mosquito nest eradication efforts, particularly mosquito screen installation and disposal of used items, can be effective in reducing the prevalence of DHF in Indonesia.
Neutrophil Extracellular Traps as Potential Therapeutic Targets in Systemic Lupus Erythematosus: A Systematic Review of Current Evidence Primartati, Danny Meganingdyah; Fatmariza, Aulia Risqi; Darmawan, Rahmad; Fadillah, Noor
Jurnal Sintesis: Penelitian Sains, Terapan dan Analisisnya Vol 6 No 2 (2025): Desember 2025
Publisher : Fakultas Sains, Teknologi, dan Analsisi Institut ilmu Kesehatan Bhakti Wiyata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56399/jst.v6i2.318

Abstract

Introduction: Systemic Lupus Erythematosus is an autoimmune disorder in which the immune system becomes increasingly dysregulated, inflamed, and involves multiple organ systems. While NETs play an essential role in antimicrobial defence, in SLE, they enhance immunopathological damage by driving type I interferon response, autoantibody production, and tissue damage. NETs, due to their unique capability of serving both as disease extraditional and as disease instigators, are a promising target in SLE. Methods: The current review is in accordance with PRISMA guidelines and registered in PROSPERO (CRD420251129386). Comprehensive searches were performed in PubMed, ScienceDirect, SpringerLink, and JSTOR, published between 2021 and 2025. Primary research investigating NETs or interventions aimed at them in SLE were included. Articles were excluded for non-originality, focus outside SLE and NETs, and lack of therapeutic focus. Documents were appraised for design, population, sample size, intervention, outcome, and therapeutic relevance for extraction. Results: A total of 1.065 articles were initially identified, of which six studies met inclusion criteria. Clinical studies consistently demonstrated elevated NET remnants, impaired NET degradation, and organ-specific deposition associated with lupus nephritis and vascular involvement. Preclinical models showed that PAD4 inhibition, DNase I supplementation, and mitochondrial-targeted antioxidants reduced NET formation, attenuated interferon-driven immune responses, and ameliorated renal and vascular pathology. Conclusion: Existing data underscore the role of NETs as critical pathogenic components of SLE and corroborate the possibility of NETs as therapeutic targets. There is, however, an immediate need for bespoke NET assays, active-signed NET biomarker stratification, NET-guided patient stratification, and rigorous clinical trial design.
Co-Authors Danny Meganingdyah Primartati Fathul Hidayatul Hasanah, Fathul Hidayatul Fita Sari, Fita Kurniawan, Angga Eka Noor Fadillah Rahmad Darmawan Sari, Nofita Sri Wahyuni