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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Journal of Fisheries & Marine
Md Yasin, Ina Salwany
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models Muhamad-Sofie, Mohd Hafiz Ngoo; Mohamad, Aslah; Azzam-Sayuti, Mohamad; Amal, Mohammad Noor Azmai; Zamri-Saad, Mohd; Monir, Md Shirajum; Md Yasin, Ina Salwany
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.59794

Abstract

Graphical Abstract Highlight Research The LD50 (median lethal dose) of Vibrio parahaemolyticus and harveyi in zebrafish was determined to be 1 x 106 CFU/mL, while for V. alginolyticus it was found to be 1 x 105 CFU/mL. The LAVh vaccine demonstrated cross-protection against various pathogenic strains of Vibrio, leading to an average of 80% survival rate in vaccinated individuals. The analysis of the LAVh vaccine emphasized its versatility, as it can be quickly deployed and stored as a freeze-dried powder. The LAVh vaccine can be easily accessible and user-friendly in various aquaculture environments, such as offshore and remote farms. Abstract Vibriosis poses a significant threat to marine teleosts, causing substantial losses in the global aquaculture industry. Previous work in our lab led to the development of a live-attenuated V. harveyi vaccine (LAVh) candidate that targets the serine endoprotease gene with a three-point knockout and has shown promise in protecting against vibriosis. However, further investigation is necessary to evaluate the stability and efficacy of its various storage conditions for broader applications. This study aims to determine how well the three different LAVh vaccine storage (fresh, stale, and freeze-dried LAVh) worked against vibriosis. A total of 1000 adult zebrafish (Danio rerio) (mean weight: 0.20±0.5 g) were divided into four groups. Groups 1, 2, and 3 were intraperitoneally injected with different LAVh vaccine storage (fresh, stale, and freeze-dried, respectively), while Group 4 received 0.01 M phosphate-buffered saline (PBS) and served as the unvaccinated control. Fish were monitored for 21 days post-vaccination for safety, stability, efficacy, and antibody analysis. The results showed that a modest dosage of 1 x 104 CFU/mL of LAVh vaccine from all storage conditions provided 80% survival upon intraperitoneal challenge with pathogenic strains of pathogenic V. harveyi, V. alginolyticus, and V. parahaemolyticus. This dosage induced significant antibody production and conferred cross-protection against different Vibrio spp., indicating the LAVh vaccine’s potential for commercial application. The LAVh vaccine demonstrated high effectiveness and suitability for storage as a freeze-dried powder. This study might offer significant insights into practical strategies for reducing vibriosis, especially in aquaculture settings with limited infrastructure.
Aktivitas antioksidan dan antibakteri ekstrak Caulerpa racemosa dengan kombinasi pelarut dan variasi waktu maserasi: Aktivitas antioksidan dan antibakteri ekstrak Caulerpa racemosa dengan kombinasi pelarut dan variasi waktu maserasi Hastuti Satyantini, Woro; Ainun Kurnia Izzata; Patmawati; Wahju Tjahjaningsih; Kiki Adi Kurnia; Md Yasin, Ina Salwany
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 11 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(11)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/wkb2jq46

Abstract

Rumput laut dari kelompok Chlorophyta dan Rhodophyta banyak dimanfaatkan di Indonesia sebagai bahan pangan. Caulerpa racemosa, salah satu jenis Chlorophyta, mengandung senyawa bioaktif antioksidan dan antibakteri di antaranya terpenoid, polifenol, alkaloid, dan flavonoid. Tujuan penelitian untuk menentukan kondisi ekstraksi optimal (konsentrasi, rasio padatan:pelarut, dan lama waktu) C. racemosa menggunakan Deep Eutectic Solvents (DES) berdasarkan aktivitas antioksidan dan antibakteri. Penelitian ini menggunakan variabel bebas (konsentrasi DES 5, 10, 15%), padatan : pelarut (1:10, 1:15, 1:20) dan lama waktu maserasi (24, 48, 72 jam). Proses optimasi dilakukan menggunakan metode Response Surface Methodology (RSM) dengan rancangan Box–Behnken melalui perangkat lunak Design Expert versi 13. Aktivitas antioksidan ekstrak C. racemosa berkisar antara 28,46±0,067 hingga 50,50±0,067 mg TE/g DW. Konsentrasi pelarut DES dan waktu maserasi berpengaruh signifikan (p < 0,05), sedangkan rasio padatan terhadap pelarut tidak menunjukkan pengaruh signifikan (p > 0,05). Aktivitas antioksidan menurun pada 15% DES akibat perbedaan polaritas dan viskositas pelarut, sementara viskositas yang lebih rendah meningkatkan efisiensi ekstraksi. Kondisi optimum diperoleh pada 10% DES, rasio 1:20, dan waktu maserasi selama 72 jam.  Aktivitas antibakteri terhadap Vibrio harveyi (13 mm) dan Vibrio parahaemolyticus (14,8 mm) juga tertinggi pada kondisi tersebut, kemungkinan karena peningkatan ekstrak senyawa bioaktif. Hasil ini menunjukkan bahwa metode ekstraksi teroptimasi ini dapat direkomendasikan untuk pengembangan produk akuakultur dan pangan fungsional.
Co-Authors Ainun Kurnia Izzata Amal, Mohammad Noor Azmai Azzam-Sayuti, Mohamad Kiki Adi Kurnia Mohamad, Aslah Monir, Md Shirajum Muhamad-Sofie, Mohd Hafiz Ngoo Patmawati Tjahjaningsih, Wahju Woro Hastuti Satyantini, Woro Zamri-Saad, Mohd