Article Per Year (5 Year)
p-Index From 2021 - 2026
1.116
P-Index
This Author published in this journals
All Journal Open Access Health Scientific Journal The Indonesian Journal of Computer Science Strada Journal of Pharmacy
Purnaningtyas, Sri Rahayu Dwi
Unknown Affiliation
Chemistry Computer Science & IT Dentistry Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Open Access Health Scientific Journal

 1 
Validation of the UV-Vis Spectrophotometric Method for the Determination of Ascorbic Acid Content in Beverage Preparations Based on a Standard Vitamin C Calibration Curve Andaririt, Devita Riafinola; Purnaningtyas, Sri Rahayu Dwi; Wijayanto, Arif
Open Access Health Scientific Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Griya Eka Sejahtera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55700/oahsj.v6i2.101

Abstract

Background: Vitamin C or ascorbic acid (C₆H₈O₆) is an essential water-soluble compound, widely used in functional beverages due to its antioxidant properties. This compound plays a role in neutralizing free radicals, strengthening the immune system, and helping the absorption of iron in the body. Packaged beverages containing vitamin C can experience degradation due to storage temperature (pH), production process, and the length of product circulation on the market. This impacts degradation of ascorbic acid and decreases the levels of vitamin C in beverage preparations, so accurate and validated analytical methods are needed to ensure the conformity of ascorbic acid levels with the label.Methods: Determination of ascorbic acid levels using a UV-Vis spectrophotometer at maximum wavelength. A standard curve was prepared from a standard vitamin C solution with a concentration of 10–18 ppm, resulting in a regression equation as the basis for calculating sample levels. Method validation included linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ) tests according to quantitative analysis guidelines.Results: The standard curve equation is Y = 0.020x + 0.030Y with r2 = 0.995r indicating good linearity. The vitamin C content in the sample is 1.045 mg/140 mL or 103.5% of the label (1000 mg), still within the Pharmacopoeia limits (90–110%). The LOD and LOQ values are 0.429 ppm and 1.3 ppm, respectively. The precision results show a %RSD of 0.1260% and an accuracy (% recovery) of 103.5%.Conclusion:The results showed that the UV-Vis spectrophotometry method had good linearity, accuracy, and precision. The vitamin C content in the product met standards. Validation parameters demonstrated that this method was consistent in analyzing vitamin C in beverage preparations.  
Molecular Docking Of Quercetin As An Anti-Breast Cancer Agent Purnaningtyas, Sri Rahayu Dwi; Andaririt, Devita Riafinola
Open Access Health Scientific Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Griya Eka Sejahtera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55700/oahsj.v6i2.126

Abstract

Background: Breast cancer is a major global health problem and a leading cause of death in women. Flavonoid compounds, particularly quercetin, have been extensively studied for their diverse pharmacological activities, including antioxidant, anti-inflammatory, and anticancer properties. Advances in computational technology in pharmaceutical and medicinal chemistry, particularly molecular docking, offer opportunities to predict interactions between ligands and target receptors quickly, efficiently, and at low cost. This study aimed to identify Molecular Docking Of Quercetin As An Anti-Breast Cancer Agent.Methods: The ligand compounds were obtained from PubChem and the receptor clusters were obtained from the Protein Data Bank. Instrumens used VivoBook_AsusLaptop X409JB Intel ® Core ™ i3-1005G1 CPU @1.20GHz 12 RAM.  The independent variables of this study were Quercetin and Doxorubicin to Cyclin-Dependent Kinase 6 (CDK6).Results: Molecular docking results show that quercetin has an average binding affinity value of –6.19 kcal/mol, while the positive control doxorubicin has an average value of –7.3 kcal/mol against the Cyclin-dependent kinase 6 (CDK6) receptor. Amino acid similarities were found in the receptors for quercetin and doxorubicin. The same bound amino acids were found on the 3NUP, 3NUX, and 4EZ5 receptors with Van Der Walls Bond. Amino acid similarities were found in the receptors for quercetin and doxorubicin. The 4NUP receptor contains the same amino acids: Phenylalanine (A:164) and Histidine (A:143), while the 3NUX receptor contains Lysine (A:26), Valine (A:45), Valine (A:27), and Phenylalanine (A:164). Meanwhile, the 4EZ5 receptor has the same amino acids, namely Glycine (A:22), Glycine (A:20), and Asparagine (A:104). This indicates a similarity in the mechanisms of action between quercetin and doxorubicin.Conclusion: Quercetin has great potential as a candidate for breast cancer treatment
Co-Authors Adjie Wahyu Pradana Arif Wijayanto Basri Noor Cahyadi Devita Riafinola La Febry Andira Rose Cynthia Mohammad Chasrun Hasani Putri, Luluk Aniqoh Meliana Syafaah, Lailis