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All Journal JURNAL ATOMIK MUKADIMAH: Jurnal Pendidikan, Sejarah, dan Ilmu-ilmu Sosial al Kimiya : Jurnal Ilmu Kimia dan Terapan
Putri, Amanda Aulia
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Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Environmental Science Social Sciences

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AKTIVITAS AMILASE PENDEGRADASI PATI MENTAH DARI BAKTERI TANAH TEMPAT PEMBUANGAN AKHIR BUKIT PINANG Putri, Amanda Aulia; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1408

Abstract

Raw starch-degrading amylase (RSDA) is a hydrolytic enzyme that can degrade raw starch into glucose. This study was conducted to obtain raw starch-degrading amylase-producing bacteria from the soil of the Bukit Pinang Landfill, Samarinda City. Isolation of bacteria using the spread plate method. Screening of RSDA-producing bacteria using nutrient media to contain raw starch. Isolation of bacteria obtained 10 single colonies of bacteria. The isolated bacteria were selected for their ability to produce RSDA. All single colonies of bacteria could produce RSDA and their activity was tested quantitatively using the 3,5 Dinitrosalicylic Acid (DNS) method. Bacteria with the TPA 1 colony code had the highest activity of 3.837 U/mL.
RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA Astuti, Winni; Adawiyah, Rabiatul; Putri, Amanda Aulia; Pratiwi, Djihan Ryn
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol 11, No 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i2.39438

Abstract

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.
RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA Astuti, Winni; Adawiyah, Rabiatul; Putri, Amanda Aulia; Pratiwi, Djihan Ryn
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol. 11 No. 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i2.39438

Abstract

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.
Hubungan Father Involvement dengan Kontrol Diri Siswa Madrasah Tsanawiyah Putri, Amanda Aulia; Zahri, Triave Nuzila; Taufik, Taufik; Sari, Azmatul Khairiah; Putra, Ade Herdian
MUKADIMAH: Jurnal Pendidikan, Sejarah, dan Ilmu-ilmu Sosial Vol 9, No 2 (2025)
Publisher : Prodi Pendidikan Sejarah Fakultas Keguruan dan Ilmu Pendidikan Universitas Islam Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/mkd.v9i2.11773

Abstract

This study aims to describe and examine the relationship between father involvement and student self-control, based on the persistent issues of truancy and low self-regulation among students. The research employed a quantitative method with a descriptive correlational approach. The study population consisted of students from MTsN 6 Padang City during the 2024/2025 academic year, with a sample of 266 students selected through stratified random sampling. The research instruments used were the father involvement scale and the self-control scale. Data were analyzed using descriptive statistics and Pearson Product Moment correlation. The results revealed that: (1) 34.96% of students exhibited a moderate level of self-control; (2) father involvement was also at a moderate level, with 33.08%; (3) there was a significant positive correlation between father involvement and students' self-control, with a correlation coefficient of 0.398. These findings affirm that father involvement makes a meaningful contribution to strengthening student self-control. This study has implications for family counseling services aimed at improving self-control among students with low levels of father involvement.
Co-Authors Azmatul Khairiah Sari Djihan Ryn Pratiwi, Djihan Ryn Putra, Ade Herdian Rabiatul Adawiyah Taufik Taufik TRIAVE NUZILA ZAHRI Winni Astuti Winni Astuti, Winni