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SKRINING FITOKIMIA DAN BIOAKTIVITAS RHODOMYRTUS TOMENTOSA (AITON) HASSK) (KARAMUNTING) Erwin, Erwin; Ilham, Muhammad Hafid; Pratiwi, Djihan Ryn; Alimuddin, Alimuddin
PROSIDING SEMINAR KIMIA Vol 2 No 2 (2024): Prosiding SNKT III 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Rhodomyrtus tomentosa (Aiton) Hassk) or known as Karamunting, is one of the medicinal plants. This medicinal plant is used by the community as a medicine for colic, fever, dysentery, sepsis, to treat shortness of breath or pain, tuberculosis, abscesses, bleeding and gynecopathy. In this study, phytochemical and bioactivity tests will be carried out on Rhodomyrtus tomentosa leaves, stem and bark extracts. Research methods include maceration of the sample with methanol, separation of the filtrate solvent obtained from the extract using a rotary evaporator, determination of chemical content using a phytochemical test, and determination of bioactivity using the brine shrimp lethality test (BSLT) method. Based on the results of phytochemical tests, it was found that the leaf extract contained alkaloids, steroids, phenolics, flavonoids, saponins and quinones. Stem extract contains alkaloids, phenolics, flavonoids, saponins and quinones. and stem bark extract contains triterpenoids, phenolics, saponins and quinones. Meanwhile, the results of the bioactivity test showed that only the leaf extract was toxic to Artemia salina shrimp with an LC50 of 582.10 ppm, while the leaf and stem bark extracts were inactive with an LC50 value above 1000 ppm. Keywords: Karamunting, BSLT test, phytochemical test, and Artemia Salina L
PENGARUH ION LOGAM TERHADAP AKTIVITAS LIPASE DARI BAKTERI AIR BEKAS GALIAN TAMBANG DI SAMARINDA Haeruddin, Haeruddin; Astuti, Winni; Pratiwi, Djihan Ryn
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Addition of metal ions on lipase activity has been done. The aim of this research is to find out the effect of metal ions on lipase activity from water bacterial post mining in Samarinda. Lipase activity was determined based on the Titrimetric method with olive oil as a substrate. The result showed lipase activity from water bacterial post mining without the addition of metal ions is 1,0 U/mL and with the addition of metal ions Mg2+, K+, Fe3+, Ca2+, dan Ba2+their respective lipase activities is 0,6 U/mL, 0,7 U/mL, 1,9 U/mL, 1,4 U/mL dan 0,8 U/mL. The conclusion that can be drawn that is metal ions K+, Mg2+ and Ba2+ inhibit lipase activity with a relative activity each at 70; 60 dan 80% while Ca2+ increase lipase activity with a relative activity at 140% and Fe3+ causes denatured lipase with a relative activity at 190%. Keywords: Bacteria, Lipase, Metal Affect
PENENTUAN VARIASI KOMPOSISI DAN pH OPTIMUM ADSORBEN KITOSAN-Fe3O4 BERDASARKAN PERSEN PENYERAPAN ION Pb2+ Lianasari, Ika Yekti; Koesnarpadi, Soerja; Pratiwi, Djihan Ryn; Munandar, Aris
JURNAL ATOMIK Vol 8 No 1 (2023)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Previously, the synthesis of Chitosan-Fe3O4 adsorbent from magnetite (Fe3O4) made from ferrous ammonium sulfate (III) and ferrous ammonium sulfate (II) in a ratio of 3:1 was carried out. The adsorbent is adjusted with the ratio of magnetite and chitosan composition 1:1; 1:2 and 2:1 with the help of glutaraldehyde as a crosslinker. The results showed that the ratio of the ideal composition is 1:1 with % absorption that is 94.97% in 24 hour, and the optimum pH is obtained at pH 5 with % absorption of 92.18% for Pb2+ ions.
UJI FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN (METODE DPPH) DARI DAUN RAMBAI (Baccaurea motleyana Mull. Arg.) Ami, Suyatmi; Saleh, Chairul; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The research about phytochemical test and antioxidant activity test on rambai leaves extract (Baccaurea motleyana Müll.Arg.) using DPPH radical immersion method had been done. This research aims to know kinds of secondary metabolite compounds in rambai leaves extract and level of antioxidant activity in rambai leaf. The result of this research showed that the secondary metabolite in rambai leaf were alkaloids, phenolics, flavonoids, saponins, steroids and triterpenoids. IC50 values obtained in each crude extract, n-hexane fraction, ethyl acetate fraction and residual ethanol fraction were 13.27 ppm, 22.81 ppm, 19.22 ppm and 13.33 ppm respectively. The highest level of antioxidant activity was in crude extracts.
SCREENING OF LIPASE FROM WATER BACTERIA IN BENANGA LEMPAKE DAM, NORTH SAMARINDA AND ITS POTENTIAL AS A DETERGENT ADDITIVE Vera, Hendrica Mini; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 8 No 2 (2023)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Lipase is an enzyme that can hydrolyze triglycerol into glycerol and free fatty acids. The aim of this research is to obtain lipase-producing bacteria and determine the potential of lipase from water bacteria in the water of the Benanga Lempake Dam, North Samarinda for biodetergent applications through stability test of lipase activity against several commercial detergents. Screening of lipase-producing bacteria was carried out on nutrient agar media containing olive oil and Rhodamine B. The results showed that seven isolates were capable of producing lipase. Based on the stability test of lipase on detergent, it can be seen that the activity of lipase decreased by more than 70% at the addition of 1% detergent.
Penurunan Konsentrasi Besi dan Mangan Terlarut pada Air Tanah Muara Lawa Menggunakan Serbuk Kiambang (Salvinia molesta) Widodo, Nanang Tri; Sari, Ika Yekti Liana; Pratiwi, Djihan Ryn
PRIMER : Jurnal Ilmiah Multidisiplin Vol. 2 No. 4 (2024): PRIMER : Jurnal Ilmiah Multidisiplin, Agustus 2024
Publisher : LPPM Institut Teknologi Dan Kesehatan Aspirasi

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Abstract

Salah satu sumber air untuk kebutuhan manusia adalah air tanah. Air tanah berfungsi memenuhi berbagai keperluan rumah tangga, seperti mandi, memasak, minum, dan mencuci. Keberadaan zat besi dan mangan di air tanah telah menjadi masalah yang serius sejak lama, kandungan besi dan mangan dapat menumbulkan rasa dan bau pada air tanah. Penelitian ini bertujuan untuk mengetahui tingkat penurunan konsentrasi besi dan mangan terlarut pada air tanah menggunakan serbuk kiambang (Salvinia molesta) sebagai adsorben. Penurunan konsentrasi besi dan mangan pada air tanah dilakukan dengan cara mengadsorpsi besi dan mangan pada air tanah menggunakan serbuk kiambang teraktifasi. Hasil penelitian menunjukkan konsentrasi besi dan mangan pada air tanah dapat diturunkan berturut-turut sebesar 64,77 % dan 77,67 %.
AKTIVITAS AMILASE PENDEGRADASI PATI MENTAH DARI BAKTERI TANAH TEMPAT PEMBUANGAN AKHIR BUKIT PINANG Putri, Amanda Aulia; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1408

Abstract

Raw starch-degrading amylase (RSDA) is a hydrolytic enzyme that can degrade raw starch into glucose. This study was conducted to obtain raw starch-degrading amylase-producing bacteria from the soil of the Bukit Pinang Landfill, Samarinda City. Isolation of bacteria using the spread plate method. Screening of RSDA-producing bacteria using nutrient media to contain raw starch. Isolation of bacteria obtained 10 single colonies of bacteria. The isolated bacteria were selected for their ability to produce RSDA. All single colonies of bacteria could produce RSDA and their activity was tested quantitatively using the 3,5 Dinitrosalicylic Acid (DNS) method. Bacteria with the TPA 1 colony code had the highest activity of 3.837 U/mL.
ANALISIS KADAR NITROGEN (N) TOTAL DAN BESI (Fe) PADA PENAMBAHAN BIOAKTIVATOR EM4 TERHADAP KOMPOS (SAMPAH KACANG PANJANG-KULIT KACANG KEDELAI) Cahyani, Amelya Putri; Sitorus, Saibun; Pratiwi, Djihan Ryn
JURNAL KIMIA MULAWARMAN Vol 22 No 1 (2024)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v22i1.1111

Abstract

Pengomposan merupakan proses penguraian bahan organik sampah dibawah kondisi-kondisi terkontrol oleh adanya aktivitas mikroorganisme pengurai. Proses pengomposan dilakukan dengan penambahan Effective microorgarnism 4 (EM4). Tahapan penelitian meliputi uji pengaruh waktu pengomposan terhadap suhu dan pH; penentuan variasi optimum bioaktivator EM4 terhadap kadar N-Total dan Fe serta waktu optimum pengomposan berdasarkan kondisi suhu, pH dan warna. Variasi optimum EM4 berdasarkan kadar N-Total pada variasi tanpa penambahan EM4 sebesar 0,4731% dan berdasarkan kadar Fe pada variasi EM4 20 mL sebesar 0,805%. Waktu optimum pengomposan berada pada hari ke-16 dengan pH 7,19-7,50; suhu sebesar 27-28,3°C dan memiliki warna hitam.
RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA Astuti, Winni; Adawiyah, Rabiatul; Putri, Amanda Aulia; Pratiwi, Djihan Ryn
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol 11, No 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i2.39438

Abstract

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.
RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA Astuti, Winni; Adawiyah, Rabiatul; Putri, Amanda Aulia; Pratiwi, Djihan Ryn
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol. 11 No. 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i2.39438

Abstract

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.