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Propolis from Mount Lawu: Dose-Dependent Antioxidant and Anti-Inflammatory Effects in a Rat Model of Wound Healing Proginova Dian Yudatama; Amru Sungkar; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 3 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i3.1232

Background: Chronic wounds present a significant healthcare challenge, necessitating the exploration of effective adjuvant therapies. Propolis, a natural product derived from beehives, has demonstrated antioxidant and anti-inflammatory properties. This study investigated the dose-dependent effects of Mount Lawu propolis extract on oxidative stress and inflammation in a rat model of wound healing. Methods: A completely randomized experimental design was employed using male rats (Rattus norvegicus). Granulation tissue wounds were induced, and the rats were treated with varying doses of ethanol extract of Mount Lawu propolis. Immunohistochemical analysis was performed to assess the expression of Malondialdehyde (MDA) and Interleukin-6 (IL-6) in the wound tissue. Results: The results demonstrated a significant dose-dependent decrease in MDA and IL-6 expression in the propolis-treated groups compared to the control group. The high-dose propolis group exhibited the most substantial reduction in both MDA and IL-6 levels. Conclusion: Mount Lawu propolis extract exhibits potent antioxidant and anti-inflammatory effects in a dose-dependent manner, suggesting its potential as an adjuvant therapy for chronic wound management.

The Role of Mount Lawu Propolis in Modulating Oxidative Stress and Angiogenic Factors During the Proliferation Phase of Wound Healing Muhammad Apri Rajab; Amru Sungkar; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1298

Background: The skin, the body's largest and first-line defense organ, is crucial for protection against environmental aggressors, thermoregulation, fluid balance, immune surveillance, sensory perception, and the intricate process of wound healing. This study investigated the modulatory role of Mount Lawu propolis extract on Malondialdehyde (MDA), a marker of oxidative stress, and Vascular Endothelial Growth Factor (VEGF), a key mediator of angiogenesis, during the critical proliferation phase of wound healing. Methods: A post-test-only control group design experimental study was conducted using twenty-four male rats (Rattus norvegicus). Following the creation of standardized full-thickness skin excision wounds, the rats were divided into four groups (n=6 per group): a control group receiving no propolis, and three treatment groups receiving topical applications of 200mg/kgBB (Group 1), 400mg/kgBB (Group 2), and 800mg/kgBB (Group 3) ethanol propolis extract daily for seven days. MDA and VEGF expression in granulated tissue samples were evaluated using immunohistochemistry and semi-quantitatively scored. Statistical analysis involved the Kruskal-Wallis non-parametric test, followed by the Mann-Whitney test due to non-normal data distribution. Results: MDA levels showed a significant reduction (p<0.05) in Group 2 (400mg/kgBB propolis) compared to the control, indicating a dose-specific antioxidant effect. VEGF expression demonstrated a significant increase (p<0.05) in all propolis-treated groups (Groups 1, 2, and 3) compared to the control, confirming the pro-angiogenic potential of propolis. Notably, the highest VEGF expression was observed in Group 3 (800mg/kgBB propolis). Conclusion: Mount Lawu ethanol propolis extract effectively modulated both oxidative stress and angiogenesis during the proliferation phase of wound healing in this rat model. A 400mg/kgBB/day dose of propolis optimally reduced MDA expression, while propolis at all doses, particularly 800mg/kgBB/day, significantly enhanced VEGF expression. These findings suggest propolis as a promising therapeutic agent to enhance wound healing by mitigating oxidative stress and promoting angiogenesis.

The Role of Mount Lawu Propolis in Modulating Oxidative Stress and Angiogenic Factors During the Proliferation Phase of Wound Healing Muhammad Apri Rajab; Amru Sungkar; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1298

Background: The skin, the body's largest and first-line defense organ, is crucial for protection against environmental aggressors, thermoregulation, fluid balance, immune surveillance, sensory perception, and the intricate process of wound healing. This study investigated the modulatory role of Mount Lawu propolis extract on Malondialdehyde (MDA), a marker of oxidative stress, and Vascular Endothelial Growth Factor (VEGF), a key mediator of angiogenesis, during the critical proliferation phase of wound healing. Methods: A post-test-only control group design experimental study was conducted using twenty-four male rats (Rattus norvegicus). Following the creation of standardized full-thickness skin excision wounds, the rats were divided into four groups (n=6 per group): a control group receiving no propolis, and three treatment groups receiving topical applications of 200mg/kgBB (Group 1), 400mg/kgBB (Group 2), and 800mg/kgBB (Group 3) ethanol propolis extract daily for seven days. MDA and VEGF expression in granulated tissue samples were evaluated using immunohistochemistry and semi-quantitatively scored. Statistical analysis involved the Kruskal-Wallis non-parametric test, followed by the Mann-Whitney test due to non-normal data distribution. Results: MDA levels showed a significant reduction (p<0.05) in Group 2 (400mg/kgBB propolis) compared to the control, indicating a dose-specific antioxidant effect. VEGF expression demonstrated a significant increase (p<0.05) in all propolis-treated groups (Groups 1, 2, and 3) compared to the control, confirming the pro-angiogenic potential of propolis. Notably, the highest VEGF expression was observed in Group 3 (800mg/kgBB propolis). Conclusion: Mount Lawu ethanol propolis extract effectively modulated both oxidative stress and angiogenesis during the proliferation phase of wound healing in this rat model. A 400mg/kgBB/day dose of propolis optimally reduced MDA expression, while propolis at all doses, particularly 800mg/kgBB/day, significantly enhanced VEGF expression. These findings suggest propolis as a promising therapeutic agent to enhance wound healing by mitigating oxidative stress and promoting angiogenesis.

Orthoplastic Limb Salvage of a Gustilo-Anderson Grade IIIB Open Trans-Articular Knee Fracture: A Case Report on Staged Reconstruction with a Rotational Fasciocutaneous Flap Satriya Teguh Imam; Amru Sungkar; Rhyan Dharma
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 9 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i9.1385

Background: High-energy, open trans-articular knee fractures with extensive soft-tissue loss represent a formidable challenge, carrying a high risk of deep infection, nonunion, and amputation. Successful limb salvage necessitates a collaborative orthoplastic approach, combining aggressive surgical debridement and skeletal stabilization with timely, vascularized soft-tissue coverage. Case presentation: A 16-year-old male was admitted after a severe traffic accident, sustaining a Gustilo-Anderson Grade IIIB open fracture of the right knee. The injury comprised a comminuted distal femur fracture, a comminuted tibial plateau fracture (Schatzker VI), a comminuted patellar fracture, and a proximal fibula fracture. A 12x15 cm soft-tissue defect with a depth of 6 cm exposed the joint capsule and all fracture sites. The patient underwent a staged management protocol. The initial surgery involved extensive debridement of non-viable tissue and stabilization with a spanning multi-planar external fixator, followed by a second-look debridement. Definitive coverage was achieved on day five with a large, medially-based rotational fasciocutaneous flap and a split-thickness skin graft over the donor site. At the 1-year follow-up, all fractures had united, and the soft-tissue envelope was stable and sensate. The patient achieved a functional knee range of motion of 0-100 degrees and could ambulate without aids. The Lysholm knee score was 85, and the Knee Society Score (KSS) was 88. Conclusion: This case report demonstrates that a meticulously planned, staged orthoplastic strategy can achieve successful limb salvage even in devastating open knee injuries. A large, local rotational fasciocutaneous flap can serve as a reliable and less complex alternative to free tissue transfer for covering massive defects in this region, particularly in young patients with favorable tissue characteristics.

Orthoplastic Limb Salvage of a Gustilo-Anderson Grade IIIB Open Trans-Articular Knee Fracture: A Case Report on Staged Reconstruction with a Rotational Fasciocutaneous Flap Satriya Teguh Imam; Amru Sungkar; Rhyan Dharma
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 9 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i9.1385

Background: High-energy, open trans-articular knee fractures with extensive soft-tissue loss represent a formidable challenge, carrying a high risk of deep infection, nonunion, and amputation. Successful limb salvage necessitates a collaborative orthoplastic approach, combining aggressive surgical debridement and skeletal stabilization with timely, vascularized soft-tissue coverage. Case presentation: A 16-year-old male was admitted after a severe traffic accident, sustaining a Gustilo-Anderson Grade IIIB open fracture of the right knee. The injury comprised a comminuted distal femur fracture, a comminuted tibial plateau fracture (Schatzker VI), a comminuted patellar fracture, and a proximal fibula fracture. A 12x15 cm soft-tissue defect with a depth of 6 cm exposed the joint capsule and all fracture sites. The patient underwent a staged management protocol. The initial surgery involved extensive debridement of non-viable tissue and stabilization with a spanning multi-planar external fixator, followed by a second-look debridement. Definitive coverage was achieved on day five with a large, medially-based rotational fasciocutaneous flap and a split-thickness skin graft over the donor site. At the 1-year follow-up, all fractures had united, and the soft-tissue envelope was stable and sensate. The patient achieved a functional knee range of motion of 0-100 degrees and could ambulate without aids. The Lysholm knee score was 85, and the Knee Society Score (KSS) was 88. Conclusion: This case report demonstrates that a meticulously planned, staged orthoplastic strategy can achieve successful limb salvage even in devastating open knee injuries. A large, local rotational fasciocutaneous flap can serve as a reliable and less complex alternative to free tissue transfer for covering massive defects in this region, particularly in young patients with favorable tissue characteristics.

Propolis from Mount Lawu: Dose-Dependent Antioxidant and Anti-Inflammatory Effects in a Rat Model of Wound Healing Proginova Dian Yudatama; Amru Sungkar; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 3 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i3.1232

Background: Chronic wounds present a significant healthcare challenge, necessitating the exploration of effective adjuvant therapies. Propolis, a natural product derived from beehives, has demonstrated antioxidant and anti-inflammatory properties. This study investigated the dose-dependent effects of Mount Lawu propolis extract on oxidative stress and inflammation in a rat model of wound healing. Methods: A completely randomized experimental design was employed using male rats (Rattus norvegicus). Granulation tissue wounds were induced, and the rats were treated with varying doses of ethanol extract of Mount Lawu propolis. Immunohistochemical analysis was performed to assess the expression of Malondialdehyde (MDA) and Interleukin-6 (IL-6) in the wound tissue. Results: The results demonstrated a significant dose-dependent decrease in MDA and IL-6 expression in the propolis-treated groups compared to the control group. The high-dose propolis group exhibited the most substantial reduction in both MDA and IL-6 levels. Conclusion: Mount Lawu propolis extract exhibits potent antioxidant and anti-inflammatory effects in a dose-dependent manner, suggesting its potential as an adjuvant therapy for chronic wound management.

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