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Faktor sosio ekonomi demografi terhadap kejadian infeksi kecacingan pada anak Sekolah Dasar Umi Mahmudah; Paramasari Dirgahayu; Brian Wasita
Ilmu Gizi Indonesia Vol 1, No 1 (2017): Agustus
Publisher : Universitas Respati Yogyakarta

Latar belakang: Infeksi kecacingan merupakan masalah kesehatan masyarakat di negara-negara berkembang seperti Indonesia. Anak sekolah dasar merupakan golongan yang paling berisiko terhadap kejadian infeksi kecacingan. Infeksi ini dipengaruhi oleh beberapa faktor yaitu kondisi sanitasi lingkungan, perilaku personal hygiene, serta kondisi sosio ekonomi demograf daerah sekitar. Kondisi sosio demograf yang berbeda di setiap wilayah mengakibatkan terjadinya infeksi kecacingan yang berbeda–beda. Tujuan: Untuk mengetahui hubungan faktor sosio ekonomi demograf terhadap kejadian infeksi kecacingan pada anak sekolah dasar. Metode: Penelitian ini merupakan penelitian observasional dengan rancangan cross sectional, pada populasi anak sekolah dasar kelas 1 sampai kelas 5 dengan jumlah 92 siswa, sedangkan besar sampel penelitian berjumlah 74 siswa di SD Barengan Kecamatan Teras Kabupaten Boyolali dengan teknik pengambilan sampel secara purposive. Data kecacingan pada anak diperoleh dengan pengambilan sampel feses dan diperiksa menggunakan metode formol ether concentration di Laboratorium Parasitologi Fakultas Kedokteran Universitas Sebelas Maret. Data sosio ekonomi demograf diperoleh dengan kuisioner dengan metode wawancara. Analisis data diolah menggunakan chi square. Hasil: Prevalensi kejadian infeksi kecacingan pada anak sekolah dasar di SD Barengan Kecamatan Teras Kabupaten Boyolali adalah 40,21%. Pendidikan ayah (p=0,159) dan pendidikan ibu (p=0,352) tidak berhubungan dengan kejadian infeksi kecacingan. Penghasilan ayah (p=0,330) dan penghasilan ibu (p=1,152) tidak berhubungan dengan kejadian infeksi kecacingan. Kesimpulan: Pendidikan ayah, pendidikan ibu, penghasilan ayah dan penghasilan ibu tidak berhubungan dengan kejadian infeksi kecacingan.

Hubungan antara Tingkat Pengetahuan dengan Pola Perilaku Penanganan Sindrom Premenstruasi di Fakultas Kedokteran Universitas Sebelas Maret David Perdana; Tedjo Danudjo; Brian Wasita
Nexus Kedokteran Komunitas Vol 1, No 2 (2012): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Premenstrual syndrome is an increased tension and sense of the complaints that usually began one week to several days before menstruation. Premenstrual syndrome affects 40% women with 5-10% of them were going to be powerless. Many opinions showed that premenstrual syndrome is an usual case, but they almost have a mistake how to handle it. This study aimed to research the relationship between the knowledge level of Premenstrual Syndrome with behavioral patterns of Premenstrual Syndrome handling. Methods: This was an analitic-observasional cross sectional study. The population was medical students 2011 Sebelas Maret University. A sample of 125 people was selected using total sampling. The researchs instrument used questionnaire about knowledge level of premenstrual syndrome and behavioral patterns of premenstrual syndrome handling. The questionnaire were pre-tested for its validity and reliability. The data were analized by use of Spearman, on SPSS 17.0. Results: Spearman analysis showed a statistically significant relationship between knowledge level and behavioral patterns of premenstrual syndrome handling (P = 0.002). Knowledge level of premenstrual syndrome was weak positive correlation with behavioral pattern of premenstrual syndrome handling (r = 0.272). Conclusions: There were weak positive and statistically significant relationship between knowledge level of premenstrual syndrome with behavioral patterns of premenstrual syndrome handling in Medical Students 2011 Sebelas Maret University. Keywords: Knowledge Level, Behavioral Patterns Handling, Premenstrual Syndrome

Pengaruh Ekstrak Nanas (Ananas Comosus (L) Merr) SEBAGAI Antihelmintik Terhadap Waktu Kematian Cacing Ascaris suum, Goeze In vitro Cindikya Saftiari Dewi; Cr. SitiUtari; Brian Wasita
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background:Pineapple (Ananas comosus (L) Merr) was reported to have a bromelain enzyme that belonged to a group of proteolytic enzymes. This enzyme has been known as anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities. The proteolytic effect of bromelain enzyme can change protein to be simple amino acid. This research was performed to understand the effect of pineapple (Ananas comosus (L) Merr) extract as anthelmintic on death time of Ascaris suum, Goeze In Vitro. Methods:This research was performed using experimental laboratory method with the post test only controlled group design. Eighty four adult Ascaris suum, Goeze were used. The worms divided into seven groups consisted of six treatment groups (pineapple (Ananas comosus (L) Merr) extract with the dose of 0 % g/ml, 3 % g/ml, 6 % g/ml, 12 % g/ml, 24 % g/ml and 48 % g/ml) and positive control group (Pirantel pamoat with the dose of 0.236% g/ml). Each group consisted of four worms. Ascaris suum, Goeze were incubated in incubator at 37C. Observation was performed every fifteen minute to determine the death time of the worms. Experimental data was analyzed using One Way ANOVA test continued with Post hoc Tukey test (? = 0.05). LC50 and LT50 were calculated using probit analysis. Results:One Way ANOVA test showed significance difference (p < 0.05). Pineapple (Ananas comosus (L) Merr) extract had a significant effect on the worm death time and showed dose dependent manner. Probit analysis showed that LC50 and LT50 of pineapple (Ananas comosus (L) Merr) extract were 21.059% g/ml and 396.929 minutes. At the concentration of 4% g/ml pineapple extract had a better effect than positive control. Conclusions:Pineapple (Ananas comosus (L) Merr) extract had an effect on the death time of Ascaris suum, Goeze which was showed by significance difference on the death time between treatment and negative control group, and this effect was comparable with positive control group. Keywords:Pineapple (Ananas comosus (L) Merr) extract, bromelain enzyme, Ascaris suum, Goeze, pirantel pamoat

Effect of EthanolicExtract of Centella asiatica againts Adrenal Gland CortexCell Lipid Droplet Feature Induced by Chronic Immobilization Stress in Rats Mochammad Fairuz Zahran; Brian Wasita; Nanang Wiyono; Ratih Puspita Febrinasari
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Chronic stress can increase the lipid droplet in cortex cell and adrenal gland damage. Pegagan extract contains such as asiaticoside, flavonoids and triterpenoid are known have antioxidant effect. The objective of the present study was to investigate the beneficial effects of Ethanolic Extract of Centella asiatica (EEC) on adrenal gland cortexcell lipid droplet feature induced by immobilization stress in male rats. Method: This study was a laboratory experimental study with randomized post test only control group design. Paraffin blocks from thirty five male rats previously subjected with chronic immobilization stress and then divided into 6 groups: control group given distilled water 1 mg/kg, stress + PGA, stress + 150 mg/kg EEC, stress + 300 mg/kg EEC, stress + 600 mg/kg EEC and stress + fluoxetine 10 mg. The treatments were performed for 21 consecutive days. Lipid droplet was evaluated using Scoring Lipid Content from Hematoxylin Eosin staining. Statistic analysis was performed using Kruskall-Wallis test continued by Post Hoc Mann-Whitney Test with Bonferroni correction. Result: Results of Kruskall Wallis test showed significant differences in six groups with p = 0.000. Mann Whitney test with Bonferroni correction showed a significant difference between control group with stress + PGA and stress + 600 mg/kg EEC. Fluexetine as positive control show the most effective dose in this study (p = 0,000). There were no significant difference between fluoxetine as control positive and control group (p = 0,112). Conclusion: Stress + 600 mg/kg EEC has beneficial effect on preventing adrenal gland cortexcell lipid droplet feature induced by chronic immobilization stress in rat. Keywords : Centella asiatica, chronic immobilization stress, adrenal glands lipid droplet feature

Pengaruh Usia terhadap Cervical Intraepithelial Neoplasia (CIN) pada Wanita dengan Infeksi Saluran Reproduksi Berbasis Pemeriksaan Papsmear Dewi Okta Anggraini; Paramasari Dirgahayu; Brian Wasita
Nexus Kedokteran Klinik Vol 2, No 1 (2013): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Cervical Intraepithelial Neoplasia (CIN) is a dysplasia of cervical squamous cells posibbly turn into cervical cancer if not treated immediately. The established cause of CIN is Human Papillomavirus (HPV). Several studies showed correlation between Reproductive Tract Infection (RTI) with CIN. But, it is still controversial. Age is one of predicted factor which can influence CIN. Through some mechanisms, age can change the cervical cells. Methods: This study was cross-sectional approach. Samples were taken from medical record, papsmears form result and papsmear preparation in the Pathology Anatomy Laboratory, Faculty of Medicine, Sebelas Maret University from 2009-2012. Total of 410 samples were divided into two groups: control and case group. Control group contain sample without RTI while case group contain sample with RTI. Each group consisted of 205 women who subsequently divided into two subgroup, aged <40 years and ? 40 years. In this study, HPV infection is uncontrolled. Data were taken including name, age, clinical symptoms, ISR and CIN. These data are analyzed using Fisher test from SPSS version 19 for windows. Results: Total CIN were found: 5 samples (<40 years) and 3 samples (?40 years) from positive RTI; 20 samples (<40 years) and 53 samples (?40 years) from negative RTI. Fisher test results showed that age has correlation with CIN (p=0,001) and there is a significant difference risk between the age distribution with CIN [RD= -12.3%, 95%CI=(-19.8)-(-4798)]. In the negative RTI, there is a significant risk sample aged <40 years and aged ?40 years to get CIN [RD= -21.28%, 95% CI= (-33.91)- (-8.655)]. RTI does not have correlation with CIN (p = 0.06) Conclusion: Without HPV controlled, age distribution (<40 years and ?40 years) has an influence to the occurence of CIN and there is no correlation between RTI with CIN. Key words: Age, Cervical Intraepithelial Neoplasia (CIN), Reproduction Tract Infection (RTI)

Pengaruh Ekstrak Buah Mengkudu (Morinda citrifolia) terhadap Waktu Kematian Cacing Ascaris suum, Goeze In Vitro Riza Deviana; Cr. Siti Utari; Brian Wasita
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Mengkudu (Morinda citrifolia) has a terapeutic effect, such as antibacterial, anthelmintic and imunomodulator. This research was performed to understand the effect of Mengkudu (Morinda citrifolia) fruit extract on death time of Ascaris suum, Goeze In Vitro. Methods: This research was performed using experimental laboratory method with post-test only controlled group design. Adult Ascaris suum, Goeze (108 worms) were divided into 9 groups. The worms treated with NaCl 0.9 % solution and Piperazine 0.4% g/ml served as negative control and positive control group consecutively. The worms treated with increasing dose of Mengkudu fruits (Morinda citrifolia) extract (1% g/ml, 2% g/ml, 4% g/ml, 6% g/ml, 8% g/ml, 10% g/ml, and 12% g/ml) served as treatment groups. Observations were performed every half hour until the worms died. LC50 and LT50 were calculated using probit analysis. Statistic analysis was performed using Kruskal-Wallis test continued by Post hoc Test with Bonferroni correction. Results: Probit analysis showed that LC50 and LT50 of Mengkudu fruits extract were 4.94% g/ml and 703.32 minutes. Kruskall-Wallis test showed that significance difference at least in the two groups. Post Hoc Mann-Whitney with Bonferroni correction test showed that treatment had significant difference (p < 0.001) to negative control and 12% g/ml group had significant difference to positive control group. Conclusions: Mengkudu (Morinda citrifolia) fruits extract can induce Ascaris suum death with the death time faster than standard medicine. Keywords: Mengkudu (Morinda citrifolia) extract, Ascaris suum, Piperazin.

EFEKTIFITAS PEMBERIAN JUS LABU SIAM (Sechium edule) TERHADAP PROFIL LIPID TIKUS (Rattus novergicus) MODEL HIPERLIPIDEMIA Yanita Listianasari; Paramasari Dirgahayu; Brian Wasita; Adi Magna Patriadi Nuhriawangsa
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) Vol. 40 No. 1 (2017)
Publisher : Persagi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/pgm.v40i1.6046.

Hyperlipidemia is a lipid profile (TC, TG, HDL, LDL) metabolism disorder becoming the leading cause of cardiovascular disease. Flavonoid, phenol, vitamin C contained in squash have strong cardioprotective effect but its optimal dose has not been known yet. The objective of this research was to analyze the effectiveness of squash juice administration with varying doses on the lipid profile of hyperlipidemia model-rat with High Fat Diet induced. This research was done in laboratory experimental research with pre and posttest control group design.The experimental animals were consisted of 36 rats and divided into negative control, positive control, treatment I (drug), treatment II, III, IV (squash juice dose 1 ml, 2 ml, 4 ml/ 100 g BW rat/day) with treatment for 38 days. The rat’s blood was taken before and after treatment for lipid profile. TC, HDL, LDL levels were measured using spectrophotometer Microlab 300 with CHOD-PAP. TG level was measured using Microlab 300 with GPO-PAP. Considering the statistic analysis, squash juice at doses 1 ml, 2 ml and 4 ml / 100 g BW rat/day, could reduce significantly (p < 0.05) TC, TG, LDL levels of rats. HDL level of rat could increase significantly (p < 0.05) with squash juice at dose 1 ml/100 g BW rat/day. There was no significant difference between the three doses of squash juice, so that squash juice at dose 1 ml/100 g BW rat/day is the best dose to reduce the lipid profile of hyperlipidemia model-rat. Hiperlipidemia merupakan gangguan metabolisme profil lipid (kolesterol total, trigliserida, HDL, LDL) yang menjadi penyebab utama timbulnya penyakit kardiovaskular. Flavonoid, fenol, vitamin C yang terkandung dalam buah labu siam mempunyai efek kardioprotektif yang kuat akan tetapi belum diketahui dosis optimal. Tujuan penelitian ini adalah untuk menganalisis efektifitas pemberian jus labu siam dengan variasi dosis terhadap profil lipid tikus model hiperlipidemia. Metode yang digunakan adalah eksperimental laboratorik dengan rancangan pre and posttest control group design. Sebanyak 36 tikus putih Strain Wistar jantan dibagi menjadi 6 kelompok dengan 6 ekor tiap kelompok, yaitu kontrol negatif, kontrol positif (diinduksi High Fat Diet), perlakuan 1 (diinduksi High Fat Diet dan obat), perlakuan 2, 3, 4 (diinduksi High Fat Diet dan jus labu siam) dosis 1 ml, 2 ml, 4 ml/100 g BB tikus/hari) dengan perlakuan selama 38 hari. Pengambilan darah tikus dilakukan sebelum dan sesudah perlakuan untuk analisis profil lipid. Kadar kolesterol total, HDL, LDL diukur menggunakan spektrofotometer Microlab 300 dengan metode CHOD-PAP. Kadar trigliserida diukur menggunakan spektrofotometer Microlab 300 dengan metode GPO-PAP. Data dianalisis menggunakan uji Paired T-Test dan menunjukkan jus labu siam ketiga dosis dapat menurunkan secara bermakna (p<0,05) kadar kolesterol total, trigliserida dan LDL tikus. Kadar HDL tikus dapat naik secara bermakna (p<0,05) dengan jus labu siam dosis 1 ml/100 g BB tikus/hari. Pemberian jus labu siam dosis 1 ml/100 g BB tikus/hari merupakan dosis yang paling efektif dalam menurunkan kadar kolesterol total, trigliserida dan LDL serta menaikkan kadar HDL.

Multiple Autoimmune Syndrome (Graves’ Disease, Autoimmune Hepatitis, SLE) in Young Male with ASD Secundum: A Rare Case Dinar Dewi Miftah Tyas Arum; Herlina Kusuma Dewi; Eva Niamuzisilawati; Aritantri Darmayani; Agus Joko Susanto; Astri Kurniati Martiana; Brian Wasita
‎ InaJEMD - Indonesian Journal of Endocrinology Metabolic and Diabetes Vol. 2 No. 1 (2025): InaJEMD Vol. 2, No. 1
Publisher : PP PERKENI

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Multiple autoimmune syndrome (MAS) is characterized by the presence of at least three autoimmune diseases, presenting complex clinical challenges due to overlapping conditions and varied manifestations. Multiple autoimmune syndrome is rarely reported in men, particularly with the combination of Graves’ disease, systemic lupus erythematosus (SLE), and autoimmune hepatitis (AIH). We report a case of a 22-year-old male who presented with symptoms of jaundice, significant weight loss, and classic signs of hyperthyroidism, including palpitations and tremors. Physical examination revealed jaundice, exophthalmos, and an enlarged thyroid gland, and he was diagnosed with Graves' disease, SLE, and AIH. Additional findings included stasis dermatitis and an atrial septal defect (ASD) with a moderate risk of pulmonary hypertension. The patient received thiamazole, propranolol, and corticosteroids, leading to clinical stabilization and symptom resolution. This rare MAS case with concurrent Graves’ disease, SLE, and AIH highlights the need for accurate diagnosis and individualized management. The immunological interplay among these diseases contributes to diverse clinical manifestations, requiring a multidisciplinary approach. Our patient’s management strategy effectively controlled hyperthyroidism, mitigated hepatic inflammation, and stabilized cardiac function, illustrating the effectiveness of comprehensive therapy. In young patients presenting with multiple autoimmune symptoms, MAS should be considered, especially with unusual combinations. Early detection and tailored treatment approaches, along with interdisciplinary collaboration, are essential to manage MAS and its associated complications.

Dynamic Coupling of Systemic and Intratumoral Estradiol in HER2-Positive and Triple-Negative Breast Cancer: A Validation Study of a Non-Invasive Surrogate Nadhim Muhammad Askar; Widyanti Soewoto; Brian Wasita
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 1 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i1.1476

Background: The role of estradiol in the tumor microenvironment (TME) of estrogen receptor (ER)-negative breast cancers is increasingly recognized. Direct measurement of intratumoral estradiol is invasive, creating a barrier to clinical research. This study aimed to determine if circulating plasma estradiol can serve as a high-fidelity, non-invasive surrogate for intratumoral concentrations in HER2-positive (HER2+) and triple-negative breast cancer (TNBC). Methods: This retrospective, cross-sectional study included 60 women with primary operable HER2+ (n=21) and TNBC (n=39) who underwent mastectomy. Paired pre-operative plasma and post-operative tumor tissue samples were analyzed. Estradiol concentrations were quantified using a validated high-performance liquid chromatography-radioimmunoassay (HPLC-RIA) protocol. Clinicopathological data, including Body Mass Index (BMI), were collected. The primary outcome was the correlation between plasma and intratumoral estradiol, assessed by Spearman's rank correlation. Paired concentrations were compared using the Wilcoxon signed-rank test. Results: Baseline clinicopathological characteristics, including BMI, were well-balanced between the HER2+ and TNBC cohorts. A highly significant, strong positive correlation was found between plasma and intratumoral estradiol concentrations across the entire cohort (Spearman's ρ = 0.78, p < 0.001). This correlation remained robust in subgroup analyses of menopausal status and tumor grade. Interestingly, median intratumoral estradiol levels (30.0 pg/mL; IQR: 10.0-65.0) were significantly lower than paired median plasma levels (132.0 pg/mL; IQR: 40.0-225.0) (p < 0.001). Conclusion: Plasma estradiol demonstrates a strong and direct correlation with intratumoral estradiol in HER2+ and TNBC, validating its use as a reliable, non-invasive surrogate. This provides a crucial tool to explore the pathophysiology of the TME. The finding that intratumoral levels are lower than systemic circulation, yet tightly coupled, suggests a dynamic equilibrium that warrants further investigation into local estradiol metabolism and signaling in ER-negative disease.

Dynamic Coupling of Systemic and Intratumoral Estradiol in HER2-Positive and Triple-Negative Breast Cancer: A Validation Study of a Non-Invasive Surrogate Nadhim Muhammad Askar; Widyanti Soewoto; Brian Wasita
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 1 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i1.1476

Background: The role of estradiol in the tumor microenvironment (TME) of estrogen receptor (ER)-negative breast cancers is increasingly recognized. Direct measurement of intratumoral estradiol is invasive, creating a barrier to clinical research. This study aimed to determine if circulating plasma estradiol can serve as a high-fidelity, non-invasive surrogate for intratumoral concentrations in HER2-positive (HER2+) and triple-negative breast cancer (TNBC). Methods: This retrospective, cross-sectional study included 60 women with primary operable HER2+ (n=21) and TNBC (n=39) who underwent mastectomy. Paired pre-operative plasma and post-operative tumor tissue samples were analyzed. Estradiol concentrations were quantified using a validated high-performance liquid chromatography-radioimmunoassay (HPLC-RIA) protocol. Clinicopathological data, including Body Mass Index (BMI), were collected. The primary outcome was the correlation between plasma and intratumoral estradiol, assessed by Spearman's rank correlation. Paired concentrations were compared using the Wilcoxon signed-rank test. Results: Baseline clinicopathological characteristics, including BMI, were well-balanced between the HER2+ and TNBC cohorts. A highly significant, strong positive correlation was found between plasma and intratumoral estradiol concentrations across the entire cohort (Spearman's ρ = 0.78, p < 0.001). This correlation remained robust in subgroup analyses of menopausal status and tumor grade. Interestingly, median intratumoral estradiol levels (30.0 pg/mL; IQR: 10.0-65.0) were significantly lower than paired median plasma levels (132.0 pg/mL; IQR: 40.0-225.0) (p < 0.001). Conclusion: Plasma estradiol demonstrates a strong and direct correlation with intratumoral estradiol in HER2+ and TNBC, validating its use as a reliable, non-invasive surrogate. This provides a crucial tool to explore the pathophysiology of the TME. The finding that intratumoral levels are lower than systemic circulation, yet tightly coupled, suggests a dynamic equilibrium that warrants further investigation into local estradiol metabolism and signaling in ER-negative disease.

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