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Aktivitas Antioksidan Ekstrak Etanol Daun dan Batang Etlingera comosa Endemik Sulawesi Tengah Menggunakan Metode ABTS Anugraini, Annisa Putri; Rasida; Maskur, Risda; Satriani; Razak, Abd Rahman; Yuyun, Yonelian
MPI (Media Pharmaceutica Indonesiana) Vol. 6 No. 2 (2024): DECEMBER
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v6i2.6827

Abstract

Etlingera comosa adalah jenis tanaman yang unik dan baru ditemukan, berasal dari Tentena, Sulawesi Tengah. Meskipun diyakini bahwa genus Etlingera ini mengandung senyawa antioksidan, belum ada penelitian yang mendalam mengenai spesies Etlingera comosa. Oleh karena itu, penelitian ini bertujuan untuk mengidentifikasi kandungan senyawa metabolit sekunder pada ekstrak etanol daun dan batang tanaman Etlingera comosa, serta mengidentifikasi aktivitas antioksidan yang terdapat pada ekstrak etanol daun dan batang Etlingera comosa. Penelitian ini menggunakan metode ABTS (2,2-azinobis 3-etilbenzotiazolin-6-sulfonat) untuk menentukan aktivitas antioksidan ekstrak etanol daun dan batang Etlingera comosa. Hasil dari penelitian menunjukkan bahwa ekstrak etanol tanaman Etlingera comosa pada bagian daun positif mengandung senyawa metabolit sekunder golongan flavonoid, alkaloid, steroid dan tanin. Sedangkan bagian batang positif mengandung senyawa metabolit sekunder golongan flavonoid, alkaloid, dan tanin. Aktivitas antioksidan ekstrak etanol tanaman Etlingera comosa berdasarkan nilai IC50 bagian daun sebesar 11,75 μg/ml sedangkan bagian batang sebesar 83,11 μg/ml. Penelitian ini menunjukkan bahwa ekstrak etanol daun dan batang Etlingera comosa mempunyai senyawa antioksidan serta memiliki aktivitas antioksidan. Etlingera comosa is a unique and newly discovered plant species endemic to Tentena, Central Sulawesi. Although the Etlingera genus is believed to contain antioxidant compounds, there has been no thorough research on the specific species Etlingera comosa. This study aims to investigate thephytochemical content of the ethanol extract from the leaves and stems of Etlingera comosa, as well as to evaluate its antioxidant activity. The research utilized the ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)) method with UV-Vis spectroscopy, measuring at a wavelength of 751 nm to assess the antioxidant activity of the ethanol extract. The findings indicated that the ethanol extract from the leaves of Etlingera comosa tested positive for secondary metabolite compounds, including flavonoids, alkaloids, triterpenoids/steroids, and tannins. In contrast, the stems showed positive results for flavonoids, alkaloids, and tannins. The antioxidant activity was quantified using the IC50 value, 11.75 μg/ml for the leaf extract and 83.11 μg/ml for the stem extract. These results demonstrate that the ethanol extracts of both the leaves and stems of Etlingera comosa contain antioxidant compounds and exhibit antioxidant activity. Submitted: 09-09-2024, Revised: 26-11-2024, Accepted: 29-11-2024, Published regularly: December 2024
Phenolic content, flavonoid, and antioxidant activity of Etlingera comosa Ardiyani and Ardi leaf extract endemic to Central Sulawesi Rasida; Anugraini, Annisa Putri; Satriani; Maskur, Risda; Widodo, Agustinus; Yuyun, Yonelian
Jurnal Ilmiah Farmasi Vol. 21 No. 2 (2025): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol21.iss2.art3

Abstract

Background: The recently identified plant species Etlingera comosa Ardiyani & Ardi is endemic to Tentena, Central Sulawesi, where it thrives both terrestrially and as an epiphyte. Nevertheless, the antioxidant activities of E. comosa have yet to be investigated, although other Etlingera species are recognized for their antioxidant components. Objective: This work aims to evaluate the antioxidant capabilities of E. comosa leaf extract using the Ferric Reducing Antioxidant Power (FRAP) assay, subsequently determining the total phenolic and flavonoid content. Method: The leaf extract was prepared through maceration with 96% ethanol. The FRAP method was employed to assess antioxidant activity, whereas the Folin-Ciocalteu method was utilized to quantify total phenolic content. The aluminum chloride colorimetric technique was employed to determine the total flavonoid content. Result: The results indicated that the extract exhibited robust antioxidant activities, with an EC₅₀ value of 9.94 µg/mL, whereas vitamin C, serving as the positive control, demonstrated an EC₅₀ value of 3.50 µg/mL. The extract comprised 18.86 mg GAE per 100 mg of extract for total phenolics and 3.31 mg QE per 100 mg of extract for total flavonoids. Conclusion: The antioxidant capacity of E. comosa leaf extract is significant, rendering it a promising subject for further pharmacological investigation.