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Toxicity assessment and bioimaging potential of carbon dots synthesized from banana peel in zebrafish model Wijayanti, Ni PAD.; Permatasari, Fitri A.; Damayanti, Sophi; Anggadiredja, Kusnandar; Iskandar, Fery; Wibowo, Indra; Rachmawati, Heni
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.1228

Abstract

Zebrafish serve as a pivotal model for bioimaging and toxicity assessments; however, the toxicity of banana peel-derived carbon dots in zebrafish has not been previously reported. The aim of this study was to assess the toxicity of carbon dots derived from banana peel in zebrafish, focusing on two types prepared through hydrothermal and pyrolysis methods. Banana peels were synthesized using hydrothermal and pyrolysis techniques and then compared for characteristics, bioimaging ability, and toxicity in zebrafish as an animal model. Pyrolysis-derived banana peel and hydrothermal-derived banana peel showed blue emission under ultraviolet light, indicating excitation-dependent behavior. To test their potential application for bioimaging, a soaking method was used using zebrafish that showed fluorescence intensity in the eyes, abdomen, and tail of zebrafish. Toxicity comparison showed that pyrolysis-derived banana peel had lower toxicity with 50% lethal concentrations (LC50) of 1707.3 ppm than hydrothermal-derived banana peel (LC50 993 ppm) in zebrafish. Both types of carbon dots showed significant differences (p<0.05) in hatching rates at 96 and 120 hours of exposure. Of the two methods for carbon dot synthesis from banana peel, the pyrolysis method had a higher toxicity threshold than the hydrothermal method, as indicated by the LC50 value and the number of zebrafish embryos that died, hatched delayed, and experienced malformation during their development.
Optimization of nSiO2-Filled RTV Silicone Rubber Coatings for Enhanced High Voltage Outdoor Insulator Performance Nalendra, Agustinus D.; Rachmawati; Alifianda, Aulia R.; Permatasari, Fitri A.; Suwarno
Emerging Science Journal Vol. 9 No. 4 (2025): August
Publisher : Ital Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/ESJ-2025-09-04-025

Abstract

This paper presents the experimental results of leakage current testing on nanosilica (nSiO₂)-filled room-temperature vulcanized (RTV) silicone rubber (SiR) coatings for outdoor insulators. Nanosilica composition is prepared based on the optimization results of surface hydrophobicity, surface resistivity, and relative permittivity of the RTV SiR matrix with varying nSiO2 contents. The study found that the insulator with a 4 wt.% nSiO₂-filled RTV SiR coating had the highest surface resistivity, better hydrophobicity, and higher permittivity compared to the unfilled RTV SiR coating. Leakage current tests are performed under several conditions (dry, clean fog, and salt fog) to evaluate the insulation characteristics of the modified RTV SiR coating applied on an actual high-voltage insulator. The results indicate that the 4 wt.% nSiO2-filled RTV SiR-coated insulator significantly reduces the leakage current magnitude and Total Harmonic Distortion (THD) when compared to that of the uncoated as well as to that of unfilled RTV SiR-coated insulators. Under all fog conditions, the 4 wt.% nSiO2-filled RTV SiR-coated insulator with a polluted surface showed the smallest leakage current THD percentage of all insulator samples. Additionally, the cross product of the leakage current magnitude and THD is also calculated to determine the condition of the insulator. The cross-product results show that the 4 wt.% nSiO₂-filled RTV SiR-coated insulator is more effective at reducing it under dry conditions, with a reduction range of 68%–81% compared to the uncoated isolator and 70%–77% compared to the unfilled RTV SiR-coated isolator. This study shows the effectiveness of RTV silicone rubber coating material with nSiO2 filler particles in reducing the magnitude of leakage current and harmonics in polluted environments.