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Profil Penggunaan Terapi Bekam di Kabupaten/Kota Bandung Ditinjau Dari Aspek Demografi, Riwayat Penyakit, dan Profil Hematologi Damayanti, Sophi; Muharini, Fitria; Gunawan, Bambang
Acta Pharmaceutica Indonesia Vol 37, No 3 (2012)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Pengobatan tradisional bekam tercatat sebagai salah satu pengobatan tradisional yang telah digunakan sejak 400 SM. Pada kurun waktu 5 tahun terakhir, pengobatan bekam di Indonesia berkembang pesat dengan ditandai berdirinya klinik-klinik dan asosiasi pengobatan bekam. Studi pra-penelitian menunjukkan bahwa rata-rata pasien yang mengunjungi klinik bekam di Bandung mencapai jumlah 4000 orang pasien setiap bulannya. Sementara itu, penelitian-penelitian yang terkait dengan terapi bekam masih terbatas terutama di Indonesia. Tujuan penelitian ini adalah untuk pendataan profil penggunaan terapi bekam di kabupaten/kota Bandung yang diharapkan dapat menjadi suatu studi pendahuluan yang mendorong dilakukannya penelitian ilmiah lain berkaitan dengan pengembangan terapi pengobatan yang lebih terjangkau bagi masyarakat. Penelitian ini merupakan studi deskriptif yang dilakukan dengan metode penyebaran kuesioner di klinik-klinik pengobatan tradisional bekam. Penelitian dilakukan selama periode bulan Januari sampai Mei 2012. Sebagai tambahan, pada penelitian ini juga dilakukan sampling pengambilan darah vena, darah bekam basah, dan darah perifer terkait dengan profil hematologi dan kadar glukosa darah sewaktu pengguna bekam basah. Terapi bekam banyak digunakan oleh masyarakat pada rentang 20-39 tahun (70,63%) dan 30-49 tahun (17,65%). Pendapatan pengguna bekam berkisar antara Rp 500.000,00-Rp 1.500.000,00 (37,5%). Latar belakang pendidikan pengguna terapi bekam berasal dari lulusan SMA. Terapi bekam di masyarakat digunakan untuk pengobatan (62%) dan menjaga kesehatan (38%). Bekam digunakan untuk mengobati tukak (30%), sakit kepala (28%), dan kolesterol (20%). Terdapat perbedaan bermakna pada (p<0,05) antara kondisi kesehatan responden sebelum dan sesudah menjalani terapi bekam terhadap intensitas kualitas tidur, kelelahan, pegal-pegal, dan intensitas sakit. Profil hematologi dan Sediaan Apus Darah Tepi (SADT) menunjukkan perbedaan komponen leukosit dan trombosit antara darah vena dan bekam.Secara statistik, perbedaan kadar glukosa darah sewaktu sebelum dan sesudah (p<0,05) tidak menunjukkan perbedaan berarti. Terapi bekam paling banyak digunakan oleh masyarakat usia 20-39 tahun (70,63%) dari kalangan ekonomi dengan pendapatan dibawah Rp 1.500.000,- (37,5%). Sebagian besar responden menggunakan terapi bekam untuk pengobatan tukak (30%). Tidak terdapat perbedaan yang berarti antara kadar glukosa darah sewaktu sebelum dan sesudah bekam.Kata kunci: Bekam, bekam basah, pengobatan tradisional, darah, profil penggunaanÂ Traditional cupping treatment is listed as one of the old traditional medicine that has been used since 400 BC. In the last 5years, treatment is rapidly growing in Indonesia by the establishment of clinics and associations of cupping treatment. Preliminary-study showed that the average patient visiting a clinic in Bandung reach the 4000 number of patients each month. However, the study of cupping therapy especially in Indonesia is still limited. The aim of this study was to collect data profile of cupping therapy in Bandung region. Moreover it is expected to be a preliminary study to encourage other scientific research related to the development of more affordable therapeutic treatments for the community. This descriptive study was done by spreading questionnaire for cupping patient at tradional medicine clinics around Bandung from January-May 2012. In addition, sampling of venous blood collection, blood cupping, and peripheral blood associated with hematological profile and blood glucose level of wet cupping users were conducted. Cupping therapy was widely used by the community in the age range of 20-39 years (70.63%) and 30-49 years (17.65%). Cupping user revenues was in a range of Rp 500,000.00 -1,500,000.00 (37.5%). Educational background of cupping therapy users came from high school graduates. In the community, cupping therapy was used for treatment (62%) and health maintaining (38%). Cupping was used to treat ulcers (30%), headache (28%), and cholesterol (20%). There were significant differences (p <0.05) between the health condition before and after cupping therapy on parameters of quality of sleep, fatigue, aches, and the frequency of experiencing pain. Hematology profile showed that, blood glucose levels before and after (p <0.05) showed no significant differences. Cupping therapy in community was used by young adult in age 20-29 years old (70.63%) with income below Rp 1,500,000.00 (37.5%). Most of respondant was used cupping therapy for the treatment of ulcers (30%). Blood glucose levels before and after (p<0.05) showed no significant difference.Keywords: Cupping, wet cupping, traditional medicine, blood, profile of use.

Determination of Sugar Content in Fruit Juices Using High Performance Liquid Chromatography Damayanti, Sophi; Permana, Benny; Weng, Choong Chie
Acta Pharmaceutica Indonesia Vol 37, No 4 (2012)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Sugar is a sweet, water-soluble, crystallizable material which is obtained commercially from sugarcane or sugar beet. As an important source of dietary carbohydrate, a sweetener, preservative in foods and a cause factor for Diabetic disease, analysis of sugar content is needed. The objective of this study was to verify the suitability and determination of sugar content in fruit juices using HPLC. The fresh fruits except strawberries were peeled, cut and blended using homogenizer. After filtration, 12.5mL of each fresh juices and packed juices were diluted with acetonitrile and distilled water (50:50). The diluted fresh juices and packed juices were loaded onto C18 Sep-Pak cartridge. Fructose, glucose, and sucrose of each fresh juices and packed juices were analyzed in HPLC with Refractive Index detector, NH2 polar bonded phase column, 10Î¼m (250mm Ã4.6mm I.D.), temperature of 43.5Â°C, mobile phase of acetonitrile and 10mM sodium phosphate (monobasic) (78:22) and flow rate of 1.0mL/min. The method validation showed good linearity of equations for fructose, glucose and sucrose were y = 3833208.4806x â 94721.0361, y = 3782886.4708x â 101683.4708, y = 3770593.9638x â 82870.9083 with regression coefficients, r2 of 0.9995, 0.9997 and 0.9996 respectively. The calculated regression function coefficients (Vx0) for fructose, glucose and sucrose were 0.0152, 0.0112 and 0.0132% respectively. The percentage recovery of fructose, glucose, and sucrose was found to be in a range of each 86.681 â 89.888, 86.898 â 90.029, and 94.541 â 97.885%. Sugar contents in fruit juices can be determined using verified HPLC method with Refractive Index Detektor. Fructose, glucose and sucrose of fresh juices contained 0.469 â 1.431, 0.454 â 1.286, 0.544 â 1.861%, whereas that of packed juices contained 0.309 â 1.587, 0.261 â 0.762,0.063 â 0.898%, respectively.Keywords: Fructose, glucose, sucrose, fruit juice, HPLC.Gula merupakan zat yang berasa manis, larut dalam air, berbentuk kristal yang dapat diperoleh dari berbagai sumber antara lain bit gula dan tebu. Sebagai sumber karbohidrat, pemanis dan pengawet dari makanan, dan penyebab diabetes, maka kandungan gula perlu ditentukan. Tujuan dari penelitian ini adalah untuk verifikasi kesesuaian penentuan kadar gula dalam jus buah menggunakan Kromatografi Cair Kinerja Tinggi (KCKT). Buah segar kecuali stroberi dikupas kemudian dipotong dan dicampur dengan menggunakan homogenizer. Setelah disaring, 12.5mL jus segar dan 12.5mL jus kemasan diencerkan dengan asetonitril dan air suling (50:50). Jus segar dan jus kemasan kemudian dilewatkan melalui tabung C18 Sep-Pak. Fruktosa, glukosa dan sukrosa kemudian dianalisis menggunakan KCKT detektor Indeks bias, kolom NH2, 10Î¼m (250Î¼m Ã ID 4.6mm) dan suhu kolom 43.5ÂºC, fase gerak asetonitril dan larutan natrium fosfat 10mM (78:22) dan laju aliran adalah 1.0mL/min. Metode validasi menunjukkan linearitas persamaan yang baik untuk fruktosa, glukosa dan sukrosa yaitu y = 3833208,4806x â 94721,0361, y = 3782886,4708x â 101683,4708, y = 3770593,9638x â 82870,9083 dengan koefisien korelasi, r2 adalah 0,9995, 0,9997 and 0,9996 masing-masing. Koefisien fungsi regresi yang dihitung (Vx0) untuk fruktosa, glukosa dan sukrosa adalah 0,0152, 0,0112 dan 0,0132 %. Persentase perolehan kembali untuk fruktose, glukosa dan sukrosa berada dalam rentang masing-masing 86,681 â 89,888, 86,898 â 90,029, dan 94,541 â 97,885%. Kandungan fruktosa, glukosa dan sukrosa dapatditentukan dengan menggunakan kromatografi cair kinerja tinggi dengan detektor indek bias. Fruktosa, glukosa dan sukrosa jus segar mengandung 0,469 â 1,431, 0,454 â 1,286, 0,544 â 1,861% sementara pada jus kemasan mengandung masing-masing 0,309 â 1,587, 0,261 â 0,762, 0,063 â 0,898%.Kata kunci: Fruktosa, glukosa, sukrosa, jus buah, HPLC.

Pengaruh Penyiapan Sampel pada Pengembangan Metode Analisis Laktosa dalam Susu Formula Menggunakan Kromatografi Cair Kinerja Tinggi Mardiana, Mardiana; Damayanti, Sophi; Ibrahim, Slamet
Acta Pharmaceutica Indonesia Vol 39, No 1 & 2 (2014)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Penelitian ini menggunakan tiga jenis prosedur preparasi sampel untuk penetapan kadar laktosa dari susu formula bayi menggunakan KCKT. Kondisi optimum KCKT adalah sebagai berikut: detektor indeks refraksi, kolom NH2, fase gerak ACNH2O (65:35) dengan laju alir 1 mL/menit. Metode ini memberikan linieritas dengan r 0,9998; koefisien variansi regresi 1,25%;Â batas deteksi 0,12 mg/mL; dan batas kuantitasi 0,35 mg/mL. Prosedur preparasi III memberikan keseksamaan dan keakuratan yang lebih baik dibandingkan prosedur I dan II, mengindikasikan bahwa prosedur preparasi sampel adalah tahap krusial pada penentuan kadar laktosa dalam susu formula bayi.Kata kunci: laktosa, susu formula bayi, preparasi sampel, kromatografi cair kinerja tinggi, detektor indeks refraksiAbstractThis research performed three different sample preparation procedure before the determination of lactose content from infant milk formula using HPLC technique under following optimum conditions: refractive index detector, NH2 column, mobile phase ACN-H2O (65:35) with the flow rate 1 mL/minute. The method gave calibration curve with r = 0.9998; regression coefficient variance 1.25%; limit of detection 0.12 mg/mL; and limit of quantitation 0.35 mg/mL. Procedure preparation III gave precision and recovery better than procedure I and II, indicating that the sample preparation is crucial step in the determination of lactose in infant milk formula.Keywords: lactose, infant milk formula, sample preparation, high performance liquid chromatography, refractive index detector

FORMULASI DAN UJI POTENSI ANTIOKSIDAN NANOSTRUCTURED LIPID CARRIER (NLC) RETINIL PALMITAT Sriarumtias, Framesti Frisma; Darijanto, Sasanti Tarini; Damayanti, Sophi
Acta Pharmaceutica Indonesia Vol 42, No 1 (2017)
Publisher : School of Pharmacy Institut Teknologi Bandung

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ABSTRAK Retinil palmitat (RP) merupakan golongan retinioid, yaitu ester vitamin A (Retinol) yang memiliki gugus palmitat pada ujung rantainya. Retinil palmitat banyak digunakan sebagai antioksidan dan anti kerut dalam industri kosmetika. Hal yang perlu diperhatikan dalam formulasi RP yaitu menjaga stabilitas dari panas, cahaya dan oksigen, sehingga perlu dicari sistem yang mampu melindungi RP dari degradasi. Salah satunya dibuat dalam sistem Nanostructured lipid carriers (NLC), sebagaimana diketahui bahwa NLC merupakan sistem penghantaran obat yang bisa meminimalisir degradasi. Tujuan penelitian ini adalah untuk mengembangkan sistem penghantaran NLC untuk meningkatkan stabilitas serta potensi antioksidan dan meningkatkan difusi RP melalui kulit. Bahan-bahan yang digunakan untuk pembuatan NLC dengan metode teknik mikroemulsi yaitu 4% PEG-8 beeswax dan 4% Isopropil miristat sebagai lipid padat dan lipid cair, 13% Polisorbat 80 sebagai surfaktan, dan 10% Sukrosa stearat sebagai kosurfaktan. Karakterisasi yang dilakukan yaitu pengukuran ukuran partikel dan polidisperistas indeks, efisiensi penjerapan, uji difusi dengan Franz diffusion cell, karakterisasi morfologi dengan Transmission electron microscopy (TEM) dan uji aktivitas antioksidan dengan DPPH. NLC-RP memiliki ukuran partikel 65,63Â±1,09 nm, indeks polidispersitas 0,32Â±0,07, efisiensi penjerapan 94,55Â±0,76%, hasil uji difusi tertinggi yaitu pada NLC-RP 52,58Â±4,37%, diikuti krim NLC-RP 36,36Â±1,46%, krim RP 18,70Â±2,13% dan emulsi RP 18,22Â±1,50%. Uji stabilitas fisika dan kimia NLC-RP disimpan selama 60 hari pada suhu 25Â°C RH 65% dan 40Â°C RH 75% menunjukan bahwa tidak ada perubahan pada kondisi tersebut. Dari penelitian ini didapat kesimpulan bahwa NLC-RP mampu menjaga stabilitas retinil palmitat. Sedangkan NLC-RP yang dimasukan kedalam krim mengalami penurunan kadar sebanyak 58,15% pada suhu ruang dan 70,05% pada suhu 40Â°C serta penurunan potensi antioksidan akibat keberadaan basis krim.Kata kunci: antioksidan, DPPH, nanostructured lipid carrier, retinil palmitatRETINYL PALMITATE NANOSTRUCTURED LIPID CARRIER (NLC) FORMULATION AND ANTIOXIDANT POTENTIAL TEST ABSTRACT Retinyl palmitate (RP), member of retinoid family is an ester of retinol with palmitate functional group at the end of the chain. RP is commonly used as antioxidants and anti-wrinkle component in cosmetic industry. RP instability requires a formulation system which makes it stable against heat, light and oxygen, such as Nanostructured Lipid Carriers (NLC). NLC is known as notable drug delivery system to minimize degradation. The aim of this research is to develop NLC delivery system to improve stability and antioxidant activity and increase RP diffusion through the skin. Materials used in NLC formulation were obtained using microemulsion technique are 4% PEG-8 beeswax and 4% isopropyl myristate as lipids in solid and liquid form, respectively; 13% polysorbate 80 as surfactant and 10% sucrose stearate as cosurfactant. Characterization NLC were evaluated using particle size measurement, polydispersity index, entrapment efficiency, in vitro diffusion testing using Franz diffusion cell, morphological characterization using Transmission Electron Microscopy (TEM) and antioxidant activity using DPPH. Particle size of NLC-RP was 65,63Â±1,09 nm, polydispersity index of 0,32Â±0,07, entrapment efficiency of 94,55Â±0,76%. Penetration result showed liquid NLC-RP difusion the highest 52,58Â±4,37%, followed by NLC-RP in cream 36,36Â±1,46%, RP in cream 18,70Â±2,13% and RP in emulsion 18,22Â±1,50%. Physical and chemical stability testing NLC-RP were stored for 60 days at 25Â°C RH 65% and 40Â°C RH 75%, the results shown there are no changed in these condition. Research results showed NLC-RP is able to maintain stability of RP, meanwhile NLC RP in cream formulation shows 58.15% amount decrease in room temperature and 70.05% amount decrease at 400C, and antioxidant activity decrease due to cream formulation.Keywords: antioxidant, DPPH, nanostructured lipid carrier, retinyl palmitate

Optimasi Reaksi Transesterifikasi Minyak Kelapa Sawit (Elaeis guineensis Jacq.) dan Penetapan Kadar Metil Ester Asam Lemak Menggunakan Kromatografi Gas Damayanti, Sophi; Yuanita, Vita; Kartasasmita, Rahmana Emran
Acta Pharmaceutica Indonesia Vol 38, No 1 (2013)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Indonesia merupakan produsen minyak kelapa sawit (Elaeis guineensis Jacq.) terbesar di dunia. Pengolahan menjadi produk turunannya dapat meningkatkan nilai ekonomi minyak kelapa sawit. Salah satu produk turunannya adalah metil ester asam lemak yang memiliki banyak kegunaan dalam industri pangan, farmasi dan kosmetik. Metil ester asam lemak dihasilkan melalui reaksi transesterifikasi minyak kelapa sawit. Penelitian ini bertujuan untuk menentukan kondisi optimum reaksi transesterifikasi minyak kelapa sawit. Optimasi diamati berdasarkan penetapan kadar metil ester asam lemak secara semikuantitatif menggunakan metode kromatografi gas. Metil ester asam lemak disintesis dari minyak kelapa sawit menggunakan katalis natrium hidroksida dan kalium hidroksida masing-masing pada suhu 40, 50 dan 60 ÂºC selama 1, 2 dan 3 jam. Metil ester asam lemak yang diperoleh dinetralkan dan dicuci. Baku metil ester asam lemak disintesis dari asam palmitat. Kadar metil ester asam lemak ditentukan menggunakan kromatografi gas dengan kolom DB-Wax 30 m x 0,25 mm, detektor FID, dan kondisi oven pada suhu 50 ÂºC selama 1 menit, kemudian dinaikkan 25 ÂºC /menit sampai 200 ÂºC, lalu dinaikkan kembali 3 ÂºC /menit sampai 230 ÂºC dan ditahan selama 18 menit. Kadar asam lemak bebas dari minyak kelapa sawit dan metil ester asam lemak ditentukan dengan metode titrasi. Rata-rata bobot hasil reaksi transesterifikasi menggunakan katalis natrium hidroksida dan kalium hidroksida adalah masing-masing 84,63Â±2,63 dan 84,25Â±4,77 g. Rata-rata kadar asam lemak bebas metil ester asam lemak hasil reaksi transesterifikasi menggunakan katalis natrium hidroksida dan kalium hidroksida adalah 0,68Â±0,03% dan 0,65Â±0,03 %. Kondisi optimum reaksi transesterifikasi minyak kelapa sawit (Elaeis guineensis Jacq.) menggunakan katalis natrium hidroksida dan kalium hidroksida masing-masing adalah pada suhu 60 ÂºC selama 1 jam, sebesar 34,20% (b/v) dan pada suhu 50 ÂºC selama 2 jam, sebesar 32,40% (b/v).Kata kunci: Transesterifikasi, minyak kelapa sawit, metil ester asam lemak, kromatografi gas, katalis basa.Â AbstractIndonesia is the largest producer of crude palm oil (Elaeis guineensis Jacq.) in the world. Processing into derived products can increase the economic value of crude palm oil. One of the derived products is the fatty acid methyl esters that have many uses in the food, pharmaceuticals and cosmetic industry. Fatty acid methyl ester is produced through transesterification reaction of crude palm oil. The aim of this research is to obtain the optimum condition of transesterification reaction of crude palm oil. Optimization was observed by the assay of fatty acid methyl esters as semi-quantitative using gas chromatography method. Fatty acid methyl esters were synthesized from crude palm oil using catalysts sodium hydroxide and potassium hydroxide respectively at 40, 50 and 60 ÂºC for 1, 2 and 3 hours. The fatty acid methyl esters obtained were neutralized and washed. The standard of fatty acid methyl ester was made by palmitic acid synthesis. The assay of fatty acid methyl esters was carried out using gas chromatography equipped with 30 m x 0,25 mm DB-Wax column, flame ionization detector (FID), using oven temperature at 50 Â°C, 1 minute, 25 Â°C /minute to 200 Â°C, 3 Â°C /minute to 230 Â°C, 18 minutes and determination of free fatty acid content from crude palm oil and fatty acid methyl esters. The average mass of the result of the transesterification reaction using sodium hydroxide and potassium hydroxide as catalyst were 84.63Â±2.63 and 84.25Â±4.77 g, respectively. The average of free fatty acid content of fatty acid methyl ester synthesized using sodium hydroxide and potassium hydroxide as catalyst were 0.68Â±0.03 % and 0.65Â±0.03 %. The optimum conditions of the transesterification reaction of crude palm oil (Elaeis guineensis Jacq.) using sodium hydroxide and potassium hydroxide as catalyst were at 60 ÂºC for 1 hour and at 50 ÂºC for 2 hours, which were 34.20% (w/v) and 32.40% (w/v), respectively.Keywords: Transesterification, crude palm oil, fatty acid methyl ester, gas chromatography, base catalyst.

Lactobacillus plantarum as Biopreservative Agent in Paneer for Dietary Food of Diabetic and Coronary Heart Disease Patients Singgih, Marlia; Ahsogan, Kanaka L.; Puspitasari, Irma M.; Damayanti, Sophi
Indonesian Journal of Clinical Pharmacy Vol 6, No 1 (2017)
Publisher : Indonesian Journal of Clinical Pharmacy

Paneer is a food product that is obtained by heating milk followed by acid coagulation. It is known asa diet food that is recommended for diabetic and coronary heart disease patients. A relatively shortershelf life of paneer is considered to be a major hurdle in its production. The present preliminary studywas conducted to determine antibacterial activity of Lactobacillus plantarum against Eschericia coliand Bacillus cereus, to develop it as biopreservative agent in paneer before using it for dietary food ofdiabetic and coronary heart disease patients in next clinical study. The antibacterial activities were testedthrough Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)using microdilution well method. Biopreservative activity in paneer was tested using total plate countmethod based on time and temperature variations. Result showed that Lactobacillus plantarum inhibitedBacillus cereus and Eschericia coli with MIC of 3125 and 1562.5 Î¼g/mL whereas MBC was in a value of>6250 and >3125 Î¼g/mL, respectively. As a biopreservative agent, addition of Lactobacillus plantarumto paneer showed no bacterial growth until 7 days in room temperature and 9th day in cold temperature. Itis concluded that Lactobacillus plantarum could be used as a natural biopreservative agent for extendingthe shelf life of paneer. The paneer with addition of Lactobacillus plantarum as biopreservative will thenbe consumed by diabetic patient and coronary heart patients in next clinical study.

ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA) Julianti, Elin; Fathurohman, Mochamad; Damayanti, Sophi; Kartasasmita, Rahmana Emran
Marine Research in Indonesia Vol 43, No 2 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum.

ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA) Julianti, Elin; Fathurohman, Mochamad; Damayanti, Sophi; Kartasasmita, Rahmana Emran
Marine Research in Indonesia Vol 43 No 2 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum.

Reseptor P2Y G-Protein Couple Receptors (GPCRs): Target Menarik Pengembangan Obat Baru Damayanti, Sophi
JURNAL FARMASI GALENIKA Vol 2 No 01 (2015): JURNAL FARMASI GALENIKA
Publisher : Sekolah Tinggi Farmasi Bandung

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P2Y purinergik reseptor adalah bagian dari G-protein couple receptor (GPCRs), group reseptor yang merupakan target utama pada berbagai pengobatan. Hingga kini, delapan subtipe reseptor P2Y telahdiidentifikasi. Kelompok pertama dari P2Yadalah reseptor P2Y1, P2Y2, P2Y4, P2Y6, dan P2Y11. Reseptor P2Yditemukan di berbagai lokasi di dalam tubuh seperti otak, limfosit, paru-paru, sel darah dan lain-lain. Salah satu reseptor yaitu reseptor P2Y12 memainkan peran utama dalam aktivasi trombosit. Reseptor ini dilaporkan untuk mengambil bagian dalam amplifikasi dan penyelesaian aktivasi platelet dan agregasi antagonis reseptor P2Y12 yang telah dikenal untuk pengobatan adalah clopidogrel dan ticlopidin. Berdasarkan lokasi dan peran P2Y reseptor dalam berbagai fungsi tubuh, dapat disimpulkan bahwa P2Y reseptor merupakan salah satu reseptor yang dapat dijadikan pengembangan obat secara rasional berdasarkan target. 

StudiIn SilicoSenyawaTurunan8-Tersubtitusi-7-Methoxy-2h-Chromen-2-OneSebagaiPenghambatEnzim Telomerase Tahir, Masdiana; Damayanti, Sophi; Hadi, Daryono; Tjahyono, Tjahyono
Jurnal Farmasi Indonesia Vol 10, No 2 (2018)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v10i2.588

Adjustment of telomere length by telomerase is considered as a biological marker which determine the proliferation of cancer cells. The telomerase activity in cancer cells is potential to be targeted for cancer therapies. The aim of this study was QSAR studies to determine the descriptors that affect the anticancer activity of 8-substituted-7-methoxy-2H-chromen-2-one derivatives. The descriptors were calculated by using software MOE® 2009.10. to design new derivative compounds that have anticancer potency, to predict pharmacokinetic properties by using preADMET, and to predict tocixity by ADMET predictorTM and interaction predict to telomerase by using Autodock 4.2.6 The results showed QSAR equation was log IC50 = -1.358 (± 1.149) - 2.957 (± 1.019) × AM1_LUMO + 0.041 (± 0.011) × E_vdw + 2.367 (± 0.748) × glob + 0.487 (± 0.100) × log S. Fourteen of the new derivative compounds were designed based on QSAR equation revealed higher activity prediction than the lead compound and performed good absorption in the intestine. Compound (33) is predicted to have low permeability in Caco-2 cells and weakly bound to plasma proteins. Compound (50) predicted weakly bound to plasma proteins as well. Toxicity prediction showed that 9 derivatives compounds were predicted to have lower nearly equal toxicity to the toxicity of the lead compound. The study of the interaction of compounds with the receptor showed that derivatives (2), (4), (40) and (41) have the highest affinity to the receptor TERT with the binding free energy is −9.60 kcal / mol, −9.39 kcal / mol, −9.20 kcal / mol and -9.08 kcal / mol. Based on QSAR study, pharmacokinetic profile, toxicity, and the study of the interaction, four compounds have the potential to be develop as anticancer with telomerase inhibition.

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