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All Journal VALENSI Jurnal Pendidikan Kimia (JPKim) Sains Natural: Journal of Biology and Chemistry
Ricson Pemimpin Hutagaol
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Nusa Bangsa University, Bogor 16166, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science

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Crystal guava (Psidium guajava L. “Crystal”) treated with gibberelic acid (GA3) : Evaluation of in vitro antioxidant capacities Fajar Sumi Lestari; Ricson Pemimpin Hutagaol; Srikandi Srikandi
Jurnal Pendidikan Kimia (JPKIM) Vol 15, No 2 (2023): August
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v15i2.41780

Abstract

Free radicals can cause many diseases, such as cancer. Antioxidant is a compound that could scavenge free radicals. One of the natural source antioxidants is guava. The application of gibberellic acid (GA3) is known to affect the physicochemical properties and antioxidant content of the fruit. The goals of this research were to investigate the antioxidant activity of skin, flesh and fruit of crystal guava were given additional GA3 treatment with concentration 0; 60; 120; 180; 240; and 300 ppm by determining the value of the Antioxidant Activity Index (AAI) using DPPH; evaluate the total phenolic content (TPC), ferric reducing antioxidant power (FRAP) and physicochemical properties; analyse the correlation between the TPC with AAI DPPH by Pearson’s method. Extraction was performed by the maceration method using acetone 50% mixed with a magnetic stirrer for 1 h. Determination of AAI DPPH, FRAP and the TPC was performed by UV-Visible spectrophotometry. TPC in the flesh and fruit fractions tended to increase and FRAP values in all fractions decreased with GA3 application. The highest TPC and FRAP was given by the G3 treatment on the skin fraction and G0 treatment on the skin fraction. The highest AAI in all fractions was given by the G3 treatment. The TPC was significantly positively correlated with the AAI DPPH of the flesh and fruit fraction extracts (0.250 ≤ r ≤ 0.880), and the skin fraction extracts showed a strong negative correlation between TPC and AAI DPPH (-0.813 < r < -0.973).Keywords: Antioxidant activity; DPPH; FRAP; Gibberellic acid; Psidium guajava
Co-Authors Ace Tatang Hidayat Agus Taufik Agus Taufiq Alvera Raisa Desi Harneti Putri Huspa Fajar Sumi Lestari I Gusti Ayu Manik Widhyastini Ihsan Rahadian Jessica Analy Khalijah Awang Lany Nurhayati Lilis Sugiarti Mamay Maslahat Nasrudin Nasrudin Niken Niken Nina Maria Santi Shanti Lestari Srikandi Srikandi Srikandi, Srikandi Tb Achyadi Ujang Pramono Unang Supratman Yoshihito shiono