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DIAMETER OOSIT KELINCI (Lepus sp.) SETELAH DIBERI PAKAN KOMERSIAL DISUPLEMENTASI MINYAK HATI IKAN KOD Ni Gusti Ayu Manik Ermayanti; Gusti Ayu Sugi Wahyuni; I Gusti Ayu Manik Widhyastini
JURNAL WIDYA BIOLOGI Vol 10 No 02 (2019): Widya Biologi
Publisher : UNHI Press

Minyak hati ikan kod sebagai sumber asam lemak tidak jenuh ganda mempunyai manfaat yang sangat beragam dan kompleks, di antaranya untuk kesehatan organ reproduksi. Penelitian ini bertujuan untuk mengetahui diameter oosit kelinci setelah diberi pakan komersial disuplementasi minyak hati ikan kod. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan empat perlakuan pakan, yaitu pakan komersial tanpa suplementasi minyak hati ikan kod (P0) sebagai kontrol, pakan komersial disuplementasi minyak hati ikan kod 3% (P1), pakan komersial disuplementasi minyak hati ikan kod 4,5% (P2), dan pakan komersial disuplementasi minyak hati ikan kod 6% (P3). Masing-masing perlakuan terdiri atas sepuluh ekor kelinci sebagai ulangan dan perlakuan diberikan mulai kelinci berumur 4 bulan sampai umur 6 bulan. Akhir perlakuan, kelinci dibedah dan selanjutnya dibuat preparathistologi ovarium. Parameter yang diamati adalah diameter oosit pada folikel primer, sekunder, tersier dan folekel praovulasi. Data yang diperoleh dianalisis dengan ANOVA, jika berbeda nyata diuji lanjut dengan DMRT. Hasil penelitian menunjukkan bahwa suplementasi minyak hati ikan kod dengan taraf yang berbeda pada pakan komersial berpengaruh nyata (P<0,05) terhadap diameter oosit kelinci. Dapat disimpulkan bahwa suplementasi minyak hati ikan kod pada taraf 4,5% pada pakan komersial mampu meningkatkan diameter oosit kelinci.

PEMANFAATAN TALAS BOGOR (Colocasia esculenta (L) Schoot) SEBAGAI LARVASIDA NYAMUK I. G. A. Manik Widhyastini; Ricson P. Hutagaol
JURNAL SAINS NATURAL Vol. 4 No. 2 (2014): Sains Natural
Publisher : Universitas Nusa Bangsa

Use of Bogor Taro (Colocasia esculenta (L)) Schoot Â as a Mosquito LarvacideÂ Â Â Â Â Â Â The case of dengue fever in Indonesia is still a major issue. Various efforts have been made towards the controllingof mosquitoes which have succeeded either chemically or naturally. Colocasia esculenta (L.)Schott (Bogor taro) have the potential to be a larvacide to exterminate mosquito larva. Taro is one of the many plants used as a source of non-rice carbohydrate that contains a high amount of nutrients, taro also contains anti nutrition .The purpose of this research was to harness waste from the Bogor taro harvest in which the use of the leaves and stem. Generally, the leaves and stem of taro is used as food for cattle as well as the presence of anti nutrition.The result of the phytochemical analysis had shown that taroÂ (Colocasia esculenta (L) Schott), consists ofÂ anti nutrition substance, other than calcium oxalate, saponin, tannin, flavonoid, thereby potentially beingÂ a larvacide. During the preliminary test, a LC50 with a magnitude of 83 ppm was obtained for the concentration of the stem extract and 61,75 ppm for the leaf extract. The leaf extract had a stronger ability than the stem extract for it can could 50% of the sample being testedKey words: larvacide, antinutritrion,metabolite secondaryÂ ABSTRAKÂ Â Â Â Â Â Â Kasus demam berdarah dengue (DBD) di Indonesia, masih sangat mengkhawatirkan. Berbagai upaya pengendalian terhadap kerapatan populasi nyamuk telah dilakukan baik secara kimia maupun alamiah. Colocasia esculenta (L.)Schott (talas Bogor) berpotensi sebagai larvasida untuk membunuh larva nyamuk.Talas merupakan salah satu tanaman sumber karbohidratÂ non beras yang bergizi tinggi. Tetapi talas juga memiliki zat antinutrisi. Penelitian ini bertujuan memanfaatan limbah hasil panen talas Bogor yang berupa daunÂ dan batang yang masih terbatas penggunaannya, umumnya digunakan sebagai makanan ternak. Hasil uji fitokimiaÂ bahwaÂ talas Bogor, mengandungÂ zat antinutrisi diantaranya, kalsium oksalat, saponin, tannin, dan flavonoid, adanya kandungan senyawa ini menunjukkan bahwa batang dan daun talas berpotensi sebagai larvasida. Pada uji pendahuluan perhitungan nilai profit diperolehÂ persamaan regresiÂ linier, peroleh hasil prediksi nilai LC 50 sebesar 83 ppm merupakan kemampuan konsentrasi dari ekstrak batang yang mampu membunuh 50 %Â larva uji dan 61,75 ppm merupakan konsentrasi dari ekstrak daun yang mampu membunuh larva uji dalamÂ hal ini adalah larva nyamuk stadium III. Berdasarkan hasil tersebut, bahwa ekstrak daun talas mempunyai kemampuan yang lebih kuat dalam membunuh 50 %Â larvaÂ Â uji daripada ekstrak batang talas.Kata Kunci: larvasida, antinutritrion,metabolite secondary

KAJIAN BERBAGAI JENIS PAKAN TERHADAP PERTUMBUHAN BENIH IKAN CUPANG BAGAN (Betta imbellis var. Sumatraensis) Asniati Asniati; I.G.A. Manik Widhyastini; Supriyono Eko Wardoyo
JURNAL SAINS NATURAL Vol. 3 No. 1 (2013): Sains Natural
Publisher : Universitas Nusa Bangsa

Study

ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.) Srikandi Srikandi; I.G.A. Manik Widhyastini
JURNAL SAINS NATURAL Vol. 4 No. 2 (2014): Sains Natural
Publisher : Universitas Nusa Bangsa

Antibacterial Extract of Mangosteen (Garcinia mangostana L.)Fruit Shell Â Â Â Â Â Â Â Â Â The skin of the mangosteen fruit is extracted with n-hexane and ethyl acetate to determine the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27 853. Results showed that n-hexane extract gave inhibition zone larger than the ethyl acetate extract on all concentrations . Extract n-hexane has a value of MIC against S. aureus ATCC bacterial test 25923 62.5 mg / ml while the ethyl acetate extract of 125 mg / ml . N- hexane extracts had MIC values of the test bacteria P.aeuroginosa ATCC 27 853 was 125 mg / ml , and while the ethyl acetate extract had a MIC value of 500 mg / ml . Treatment of solvent, concentration and interaction between the solvent and concentration significantly affected the test bacteria ATCC 25923 S. aureus at the level of 5 %, the highest interaction N-Hexane solvent with a concentration of 15,625 mg / ml and was not significantly different interactions with the concentration of 31.25 mg/ml and 125 mg/ml. Treatment solvent and concentration significantly while the interaction between the solvent and the concentration has no effect on the test bacteria P.aeuroginosa ATCC 27 853 at 5% level .Keywords: Garcinia mangostana L., Staphylococcus aureus and Pseudomonas aeruginosaÂ ABSTRAKÂ Â Â Â Â Â Â Â Â Â Kulit buah manggis diekstrak dengan n-heksan dan etil asetatÂ Â untuk mengetahui Konsentrasi Hambat Minimum (KHTM) terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aeruginosa ATCC 27853. Hasil penelitian menunjukkan bahwa ekstrak n-heksana Â memberikan zona hambatan lebih besar dibandingkan dengan ekstrak etil asetat pada semua konsentrasi. Ekstrak n-heksanaÂ memiliki nilai kadar hambat minimal (KHM) terhadap bakteri uji S. aureus ATCC 25923Â 62,5 mg/ml sedangkan ekstrak etil asetat 125 mg/ml.Â Â Ekstrak n-heksana memiliki nilai KHMÂ Â terhadap bakteri uji P.aeuroginosaÂ ATCC 27853 adalah 125 mg/ml danÂ sedangkan ekstrak etil asetat memiliki nilai KHMÂ 500 mg/ml. Perlakuan pelarut, konsentrasi dan interaksi antara pelarut dan konsentrasiÂ berpengaruh nyata terhadap bakteri uji S. aureus ATCC 25923 pada taraf 5%, interaksi tertinggi yaitu pelarut N-Heksan dengan konsentrasi 15,625mg/ml dan interaksi ini tidak berbeda nyata dengan konsentrasi 31,25 mg/ml dan 125 mg/ml. Perlakuan pelarut dan konsentrasiÂ berpengaruh nyata sedangkan interaksi antara pelarut dan konsentrasiÂ tidak berpengaruh terhadap bakteri uji P.aeuroginosaÂ ATCC 27853 pada taraf 5%.Â Kata kunci: Â Garcinia mangostana L., Staphylococcus aureus dan Pseudomonas aeruginosa

AKTIVITAS ANTIBAKTERI INFUSA DAUN LIDAH BUAYA (Aloe vera L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli Yuni Widyastuti; Nia Yuliani; I.G.A. Manik Widhyastini
JURNAL SAINS NATURAL Vol. 6 No. 1 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Antibacterial Activity of Infused Aloe Vera Leaf (Aloe Vera L) on Growth Of Stapylococcus aureus and Escherichia coliDisinfectants may act as antibacterials. Aloe vera (Aloe vera L) is one of the plants that is often used by the community as a medicinal plant that has many active compounds such as lignin, saponin and anthraquinone substances that act as antibacterial. This study aims to determine the antibacterial activity of Aloe vera leaf extracted with infusa method on the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is to see the reduction / reduction of bacterial count (% reduction) after 30 second and 60 second with disc diffusion method. The results showed decrease or decrease of bacteria count (% reduction) to Escherichia coli bacteria found in aloe leaf skin infected by 28,2% after 60 seconds. While decrease or decrease of bacteria amount (% reduction) to Staphyloccocus uareus bacteria infusa mixture of meat and skin of Aloe vera leaves of 27.5% after 60 seconds. Antibacterial test of aloe vera leaf aloe (Aloe veraL) of 100% concentration by disc diffusion method did not show any inhibition zone around cultures of test bacteria.Keywords: Aloe vera, antibacterial activity, Staphylococcus aureus, and Escherichia coli, Infusa.ABSTRAKDesinfektan dapat berfungsi sebagai antibakteri. Lidah buaya (Aloe vera L) merupakan salah satu tanaman yang sering digunakan masyarakat sebagai tanaman obat yang memiliki banyak senyawa aktif seperti lignin, saponin dan antrakuinon yaitu zat yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri daun lidah buaya (Aloe vera L) yang diekstrak dengan metode infusa terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Metode penelitian yaitu dengan melihat penurunan/pengurangan jumlah bakteri (% reduksi) setelah waktu 30 detik dan 60 detik dengan metode difusi cakram. Hasil penelitian menunjukkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Escherichia coli terdapat pada infusa kulit daun lidah buaya yaitu sebesar 28,2 % setelah waktu 60 detik.Sedangkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Staphyloccocus uareus terdapat pada infusa campuran daging dan kulit daun lidah buaya yaitu sebesar 27,5 % setelah waktu 60 detik. Uji antibakteri infusa daun lidah buaya (Aloe veraL) konsentrasi 100% dengan metode difusi cakram tidak menunjukkan adanya zona penghambatan disekitar biakan bakteri uji.Kata kunci : Lidah buaya, Aktivitas antibakteri, Staphylococcus aureus, danÂ Â Escherichia coli,Â Â Infusa.

PENGARUH SUHU PENGERINGAN KULIT BAGIAN LUAR DAN BAGIAN DALAM MANGGIS (Gracinia mangostana, L.) TERHADAP KANDUNGAN PROKSIMAT, ALKALOID DAN FLAVONOID I. G. A. Manik Widhyastini
JURNAL SAINS NATURAL Vol. 3 No. 2 (2013): Sains Natural
Publisher : Universitas Nusa Bangsa

The Effect of Drying on The Outer and Inner Skin of Mangosteen (Gracinia mangostana, L.) for the Content of Proximate Analysis, Alkaloid and Flavanoid Â Â Â Â Â Â Â Â Mangosteen (Garcinia mangostana,L.), is considered to be one of the leading exports commodity in which the peel has the major part (60,82% from the total weight of the fruit), the flesh of the fruit is the second (35,51), the rest are the leaf sheaths (3,67). The peel of the mangosteen is rich in antioxidant which is useful for human health and this potential hasnâ€™t been used widely. The use of the peel of mangosteen need to be furtherly reviewed.The preliminary research aimed to figure out the optimum temperature for drying the outer and the inner skin of the mangosteen as a test to obtain the content of proximate analysis, alkaloid and flavanoid. The results of the proximate test was obtained that the water content of the outer skin with temperature of 70oC ;80oC; 90oC ;100oC, was between 7% to 10% while the water content for the inner skin was between 11% to 12%, dry mass of 1% to 3%, N content between 0,1% until 0,5%, carbohydrate content between 1% until 2,5%. The results of the alkaloid test showed that all of the experiment treatments revealed positive results as well as the flavonoid test. The highest content of alkaloid and flavonoid was obtained at a temperature ofÂ 80oC to dry the outer skin of the mangosteen.Key words: proximate analysis, antioxidant ,alkaloid and flavonoid Â ABSTRAKÂ Â Â Â Â Â Â Manggis (Garcinia mangostana,L.), merupakan salah satu primadona ekspor yang menjadi andalan Indonesia Komponen terbesar dari buah manggis adalah kulit buah (60,82% dari berat buah utuh),daging buah merupakan komponen yang kedua (35,51% ), sisanya adalah komponen daun kelopak (3,67 % ). Kulit buah manggis, kaya akan senyawa antioksidan yang sangat bermanfaat bagi kesehatan manusia dan potensi ini belum dimanfaatkan secara luas.PemanfaatanÂ kulit buah manggis perlu dikaji lebih lanjut.Penelitian pendahuluan yang bertujuan untuk mengetahui suhu pengeringan optimalÂ padakulit bagian luar dan kulit bagian dalam manggis terhadap kandunganproksimat, alkaloid dan flavonoid. Hasil uji proksimat diperoleh Â bahwa kadar air untuk kulit bagian luar masing-masing dengan suhu pengeringan 70oC ;80oC; 90oC ;100oCÂ adalah berkisar dari 7 % sampai 10 % sedangkan kadar air pada kulit bagian dalam adalah berkisar dari 11 % sampai 12 %, kadar abu 1% sampai 3 %, kadar N berkisar antara 0,1 % sampai 0,5 %, kadar Karbohidrat berkisar antara 1% sampai 2,5 %. Hasil uji alkaloid menunjukkan bahwa semua perlakuan menunjukkan hasil positif demikian juga dengan hasil uji flavonoid. Kandungan alkaloid dan flavonoid terbanyak diperoleh pada pengeringan 80oC untuk kulit bagian luar dari manggis.Kata kunci: proksimat, antioksidan, alkaloid dan flavonoid

Inhibitory Capacity of Clay Mask 96% Ethanol Extract from Bitter Melon (Momordica charantia L.) Against Staphylococcus aureus Siti Qur'aniati; I Gusti Ayu Manik Widhyastini; Devy Susanty
JURNAL SAINS NATURAL Vol. 12 No. 3 (2022): Sains Natural
Publisher : Universitas Nusa Bangsa

Momordica charantia L. commonly known as bitter melon plant, is widely used as a treatment for various diseases, including skin infections. The purpose of this study was to determine the highest concentration of bitter melon extract and infusion in inhibiting the growth of Staphylococcus aureus bacteria. This research was conducted using the disc diffusion method and evaluation of the quality of the clay mask was carried out on the growth of Staphylococcus aureus bacteria. The results showed that 96 % ethanol extract of bitter melon at a concentration of 100% had an inhibitory effectiveness of 6.32 mm and was higher than that of 4.85 mm of bitter melon infusion. The clay mask formula of 96% ethanol extract of bitter melon with concentrations of 5 % (F1), 7.5 % (F2) and 10 % (F3) gave a fairly good evaluation of physical quality with an inhibition zone of 5.0 mm; 7.06 mm and 7.30 mm, respectively. The clay mask of 96% ethanol extract of bitter melon fruit F1, F2 and F3 belongs to the medium category in inhibiting bacteria.keywords:Â inhibition;Â ethanol extract;Â clay mask;Â Momordica charantia L.;Â Staphylococcus aureusABSTRAKDaya Hambat Masker Clay Ekstrak Etanol 96% Buah Pare (Momordica charantia L.) terhadap Bakteri Staphylococcus aureusMomordica charantia L. umumnya dikenal dengan tanaman pare, digunakan sebagai pengobatan terhadap berbagai penyakit, diantaranya infeksi pada kulit. Penelitian ini bertujuan untuk mengetahui konsentrasi tertinggi ekstrak dan infusa buah pare dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Penelitian ini dilakukan menggunakan metode difusi cakram dan evaluasi mutu masker clay dilakukan terhadap pertumbuhan bakteri Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak etanol 96 % buah pare pada konsentrasi 100 % memiliki efektivitas daya hambat sebesar 6,32 mmÂ dan lebih tinggi dibandingkan dengan infusa buah pare 4,85 mm. Formula masker clay ekstrak etanol 96 % buah pare konsentrasi 5 % (F1) ; 7,5 % ; (F2) dan 10 % (F3) memberikan hasil evaluasi mutu fisik yang cukup baik dengan zona hambatÂ berturut-turut 5,0 mm ; 7,06 mm dan 7,30 mm. Masker clay ekstrak etanol 96% buah pare F1, F2 dan F3 termasuk ke dalam kategori sedang dalam menghambat bakteri.Kata kunci: daya hambat; ekstrak etanol; masker clay; Momordica charantia L.; Staphylococcus aureusÂ

Blood Glucose Level and Pancreas Histological Section of Male Rabbits after Being Fed Commercial Feed Supplemented with Cod Liver Oil Ni Gusti Ayu Manik Ermayanti; Ida Bagus Made Suaskara; I Gusti Ayu Manik Widhyastini; Iriani Setyawati
JURNAL SAINS NATURAL Vol. 13 No. 1 (2023): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v13i1.441

Fish oil contains eicosapentaenoic acid (E.P.A.) and docosahexaenoic acid (D.H.A.) which are beneficial for heart health, controlling blood glucose, reducing atherosclerosis, and lowering the risk of cancer. This study aims to determine the effect of commercial feed supplemented with cod liver oil on blood glucose levels and pancreatic histology of male rabbits. Twenty rabbits were divided into four groups, namely commercial feed supplemented with cod liver oil 3% (P1), 4.5% (P2), 6% (P3), and commercial feed without cod liver oil supplementation (control). Each group consisted of five rabbits, and the treatment was given for four weeks. The parameters observed were blood glucose levels in the 1st, 2nd, 3rd, 4th, and 5th weeks and the histology of the male rabbitsâ€™ pancreas. Data were analyzed by Anova. The results showed that in the 1st, 2nd, and 3rd weeks there was no significant difference (P>0.05). In contrast, in the 4th Â and 5th weeks, there was a significant difference (P<0.05) between the control and treatment of cod liver oil supplementation on blood glucose levels in male rabbits, and the observational results on the histology of rabbits showed normal pancreatic histology. In conclusion, cod liver oil supplementation on commercial feed controlled blood glucose levels, and there was no disturbance in the histological structure of the pancreas of male rabbits.Keywords: fatty acid, glucose, cod liver oil, pancreas, rabbit

Daily Behavior of Binturong (Arctictis binturong) in Ex-situ Conservation Taman Margasatwa Ragunan Widya Anggraini; Mia Azizah; I.G.A Manik Widhyastini
JURNAL SAINS NATURAL Vol. 13 No. 2 (2023): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v13i2.471

Binturong (Arctictis binturong) is a mammal in the family of Viverridae, which includes animals such as civets. Binturong is a species with VU (Vulnerable) status, which means that it faces the risk of extinction in the wild in the future based on the International Union for Conservation of Nature and Natural Resources (IUCN) Red List and has Appendix III based on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). This research aimed to determine the behavior at Taman Margasatwa Ragunan (Ragunan Wildlife Park) from July to September 2022. Taman Margasatwa Ragunan is an ex-situ conservation for flora and fauna, which is also helpful as a place for research, education, and recreation. This research determined the behavior, frequency, and duration of binturong’s daily activities at Taman Margasatwa Ragunan (Ragunan Wildlife Park). This study used a scan sampling method, and the objects were three individual binturongs, namely male Sumatran binturong (Martin), male Kalimantan binturong (Amoy), and female Kalimantan binturong (Gaza). The results obtained showed that the highest activity was found in resting behavior (6444 minutes; 82,64%) and the lowest was in agonistic behavior (3 minutes; 0,03%) carried out by Martin, then the highest activity was in resting behavior (6607 minutes; 84,75%) and the lowest was the behavior of defecation (5 minutes; 0,06%) carried out by Amoy, then the highest activity was in resting behavior (6833 minutes; 87,61%) and the lowest was the behavior of defecation (1 minute; 0,01%) carried out by Gaza.

Hemoglobin Levels and Number of Erythrocytes in Scabies-infected Male Mice (Mus musculus) Treated with Water Extract of Taro (Colocasia esculenta (L.) Schott Cultivar Hideung Widhyastini, I Gusti Ayu Manik; Nia Yuliani; Ananda Sarah Nur Azizah
Sains Natural: Journal of Biology and Chemistry Vol. 14 No. 1 (2024): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v14i1.694

This study aims to analyze the hemoglobin levels and number of erythrocytes in scabies-infected male mice treated with taro water extract as a cheap and safe alternative treatment for scabies. The test animals were 24 mice weighing 20-25 grams, divided into 6 groups consisting of: K1: taro water extract 100gr/100 ml water, K2 taro water extract 200gr/100ml water, K3: using 300gr/100ml taro water extract water, K4: ivermectin positive control, K5: negative control with water alone, and K6 normal control not infected with scabies and not given treatment. The application of taro extract was carried out for five days. Observations were carried out every day, until day 7. Blood samples were taken from mice three times: before being infected with scabies (healthy), when infected with scabies, and after being treated with taro water extract. The data obtained was processed statistically using the SPSS software with the One Way ANOVA test. Sarcoptes scabiei infection affects the appearance of the blood profile of male mice when infected, there is an increase in the number of erythrocytes but does not affect hemoglobin levels. Treatment with water extract of the taro (Colocasia esculenta (L.) Schott) cultivar hideung in different concentrations did not show significant blood results, but it could heal wounds caused by scabies infection.

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