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Study of Protein Hydrolysis and Peptide Antioxidants Activity of Chicken Slaughterhouse Waste and Its Potential for Feed Additives Hartoyo, Bambang; Widyastuti, Titin; Rahayu, Sri; Santosa, R Singgih Sugeng
ANIMAL PRODUCTION Vol. 24 No. 2 (2022)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2022.24.2.133

Ensuring food safety in livestock requires specific feeding technology in agriculture by using feed additives in the form of antibiotics, prebiotics, probiotics, acidifiers, hormones and enzymes. Bioactive peptides improve the health status of humans and animals. Protein hydrolysis produce peptides that are safe, quickly metabolizable, less risky for livestock products to have contaminant residue. Bioactive peptides are still bound to the original protein, so they need to be released through an enzymatic process. This research explored the potential of chicken slaughterhouse waste to produce biopeptides by hydrolyzing proteins using various proteolytic enzymes. The slaughterhouse waste included chicken feet, intestines, filleting waste and blood plasma. The proteolytic enzymes used were papain, bromelain, protease by Rhizopus oligosphorus, probiotic protease. The observed variables were dissolved protein content with and without precipitation, protein hydrolyzate content, and the degree of enzyme hydrolysis. The research was conducted using exploratory methods. The results showed that the dissolved protein content in the chicken slaughterhouse waste protein concentrate was 1,585 mg/ml (feet), 2,361 mg/ml (intestines), 1,787 (filleting waste) and 2,372 mg/ml (blood plasma). Blood plasma protein concentrate showed the highest yield among other chicken slaughterhouse waste protein concentrates, namely 0.14 mg/ml (hydrolysis of papain), 0.18 mg/ml (hydrolysis of bromelain), 0.56 mg/ml (hydrolysis of R.oligosphorus protease) 0.68 mg/ml (hydrolysis of probiotic proteases). The highest degree of hydrolysis was shown in blood plasma hydrolyzates using probiotic protease enzymes, namely 28.72%. The highest antioxidant activity was 92.92% as observed in chicken feet protein hydrolyzate which was hydrolyzed using papain. Therefore, chicken feet, intestines and fillet waste can produce protein concentrates through precipitation using ammonium sulfate, and plasma using acetone. The highest protein concentration was in blood plasma protein which also produced the highest hydrolysis from hydrolyzing blood plasma proteins with hydrolyzed probiotic protease. The highest antioxidant activity was observed in chicken feet protein hydrolyzate which was hydrolyzed using papain enzyme and incubated for 6h.

Performance Test to Select Female Tegal Ducks Based on Production Characteristics Purwantini, Dattadewi; Santosa, R Singgih Sugeng; Santosa, Setya Agus; Susanto, Agus; Candrasari , Dewi Puspita
ANIMAL PRODUCTION Vol. 26 No. 1 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.1.192-1

This study aims to determine the increase in production capacity through genetic quality improvement using selection techniques with the production capability test method in Tegal ducks. The research was conducted using an experimental method, using a nested pattern experimental design, male as treatment, female as sub-treatment, offspring as replicates, and production characteristics as observations. The selection population of Tegal ducks consisted of 10 males, 50 females, and their offspring. Production characteristics recorded included egg weight, hatching weight, and egg production. Egg production measured was Hen Day Production (HDP) at the initial laying period for 90 days. Assessment of genetic quality using individual breeding values (BV). Each individual's breeding value is calculated, and then the estimated breeding value (EBV) results are arranged based on their rank. Selection of parent candidates is done by maintaining 25, 50, and 75% of the total population. Selection results are obtained by estimating the selection response using different selection intensities. The results showed that the average and standard deviation of the characteristics of egg weight, hatching weight, and percentage of egg production in Tegal ducks were 67.76 ± 4.57 g, 40.40 ± 2.16 g, and 63.33 ± 10.89 %, respectively. Heritability values (h2) and standard error of egg weight characteristics, hatching weight, and percentage of egg production in Tegal ducks were 0.47 ± 0.032, 0.39 ± 0.0589, and 0.512 ± 0.071, respectively. The assessment for selection response was conducted based on three factors - egg weight, hatching weight, and percentage of egg production each week. The proportions were maintained at 25%, 50%, and 75%, respectively. The egg weight proportions were 0.46, 0.29, and 0.15, while the hatching weight proportions were 0.71, 0.45, and 0.23, respectively. The percentage of egg production each week was 47.92%, 30.18%, and 15.85%, respectively. The study’s results revealed that the intensity of selection and selection response of the Tegal duck were higher when smaller proportions were used in the selection process. It should be noted that the production characteristics used in the selection process were different, which resulted in varying heritability values and selection responses. Overall, these findings suggest that careful consideration of the selection factors is necessary to achieve optimal results in the selection process of the Tegal duck

Enhancing Blood Profile and Performance of Broiler Supplemented with Biopeptide from Chicken Feet Hartoyo, Bambang; Widyastuti, Titin; Rahayu, Sri; Santosa, R Singgih Sugeng
ANIMAL PRODUCTION Vol. 25 No. 3 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.3.234

Feed manipulation by feeding additive aimed to produce safety and healthy livestock product. Bioactive peptide from chicken feed as one of solution to increase immune function without give any effect to host. This study aimed to evaluate addition of biopeptide from chicken feed to blood profile and performance of broiler. Two hundred one-day-old commercial broiler chicken and assigned to four treatments (0, 2, 4, and 6 %) with ad libitum water during research to evaluate treatment to parameter tested such as feed consumption, weight gain, leucocyte, lymphocyte, erythrocyte and monocyte. Five replication and ten trial unit of broiler used. Addition biopeptide from chicken feed until 6 % did not give any effect (p>0.05) to feed consumption, but there was significant (p<0.05) effect on daily weight gain, statistically. Meanwhile, 40 % dosage of biopeptide from chicken feed increased (p<0.05) quantity of lymphocyte of broiler. It is concluded that the addition of biopeptide enhanced immune of broiler without give any negative effect of broiler performance

Study of Protein Hydrolysis and Peptide Antioxidants Activity of Chicken Slaughterhouse Waste and Its Potential for Feed Additives Hartoyo, Bambang; Widyastuti, Titin; Rahayu, Sri; Santosa, R Singgih Sugeng
ANIMAL PRODUCTION Vol. 24 No. 2 (2022)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2022.24.2.133

Ensuring food safety in livestock requires specific feeding technology in agriculture by using feed additives in the form of antibiotics, prebiotics, probiotics, acidifiers, hormones and enzymes. Bioactive peptides improve the health status of humans and animals. Protein hydrolysis produce peptides that are safe, quickly metabolizable, less risky for livestock products to have contaminant residue. Bioactive peptides are still bound to the original protein, so they need to be released through an enzymatic process. This research explored the potential of chicken slaughterhouse waste to produce biopeptides by hydrolyzing proteins using various proteolytic enzymes. The slaughterhouse waste included chicken feet, intestines, filleting waste and blood plasma. The proteolytic enzymes used were papain, bromelain, protease by Rhizopus oligosphorus, probiotic protease. The observed variables were dissolved protein content with and without precipitation, protein hydrolyzate content, and the degree of enzyme hydrolysis. The research was conducted using exploratory methods. The results showed that the dissolved protein content in the chicken slaughterhouse waste protein concentrate was 1,585 mg/ml (feet), 2,361 mg/ml (intestines), 1,787 (filleting waste) and 2,372 mg/ml (blood plasma). Blood plasma protein concentrate showed the highest yield among other chicken slaughterhouse waste protein concentrates, namely 0.14 mg/ml (hydrolysis of papain), 0.18 mg/ml (hydrolysis of bromelain), 0.56 mg/ml (hydrolysis of R.oligosphorus protease) 0.68 mg/ml (hydrolysis of probiotic proteases). The highest degree of hydrolysis was shown in blood plasma hydrolyzates using probiotic protease enzymes, namely 28.72%. The highest antioxidant activity was 92.92% as observed in chicken feet protein hydrolyzate which was hydrolyzed using papain. Therefore, chicken feet, intestines and fillet waste can produce protein concentrates through precipitation using ammonium sulfate, and plasma using acetone. The highest protein concentration was in blood plasma protein which also produced the highest hydrolysis from hydrolyzing blood plasma proteins with hydrolyzed probiotic protease. The highest antioxidant activity was observed in chicken feet protein hydrolyzate which was hydrolyzed using papain enzyme and incubated for 6h.

KAJIAN KADAR AIR, WAKTU LARUT DAN RENDEMEN TEPUNG CANGKANG TELUR ITIK DENGAN METODE DAN WAKTU PEMASAKAN YANG BERBEDA Nurmala, Nita; Santosa, R Singgih Sugeng; Setyaningrum, Agustinah
ANGON: Journal of Animal Science and Technology Vol 3 No 2 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Background.This study aims to determine the interaction between cooking method and time, and the effect of cooking method and time on water content, dissolving time, and yield of duck eggshell flour. Materials and Methods. The materials used were 240g duck eggshells and aquadest. This study used an experimental method with a completely randomized design with a factorial pattern (2x4) consisting of 2 factors. Factor A=Cooking Method (M) with a factor level M1= Boiling Method and M2=Steaming Method, Factor B=Cooking Time (L) with a factor level L1=Cooking 0 minutes, L2=Cooking 10 minutes, L3=Cooking 20 minutes and L4=Cooking for 30 minutes, the treatment combination will be repeated 3 times. Data were analyzed using analysis of variance followed by further Orthogonal Polynomial and BNJ tests if they had a significant effect. Results. The results showed that the interaction between cooking method and time had a significant effect on water content, but had no significant effect on dissolving and yield time. The mean of research results for water content was 0.43%±0.25- 2.4%±0.66. The cooking method had a significant effect on dissolving time, but had no significant effect on water content and yield. The mean of research results for dissolving time was 8.81±0.38 seconds – 10.93±0.66 seconds. Cooking time had a significant effect on water content and yield, but had no significant effect on dissolving time. The average yield of the study was 97.53%±0.47-98.87%±0.06. Conclusion. The conclusion of this research is to produce good duck eggshell flour using the boiling method for 30 minutes, with the average result of water content of 1.03%±0.61, dissolving time of 7.75±1.67 seconds and yield of 98.2%±0.20.

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