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Role of plant growth promoting rhizobacteria on Pteris vittata L as a potential hyperaccumulator plant for gold phytomining agent Aminatun, Tien; Rakhmawati, Anna; Atun, Sri; Idrus, Arifudin; Sujangka, Arsyad
Journal of Degraded and Mining Lands Management Vol. 12 No. 2 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.122.7217

Abstract

Gold phytomining is the extraction of gold from the soil by harvesting specially selected hyperaccumulator plants. One of the potential plant species as a gold hyperaccumulator at the Ratatotok site, North Sulawesi, Indonesia, is Pteris vitata L, possibly because of the presence of rhizobacteria colonies in the roots to help the plant's resistance to metal stress in the soil. The isolation and identification show that the most resistant rhizobacteria to the gold stress is Pseudomonas aeruginosa RTKP1. The study aimed to assess the Pseudomonas aeruginosa RTKP1 to assist the gold bioaccumulation in Pteris vittata L. The phytomining test was carried out with four series of treatments for Pteris vittata L growing media, i.e., (1) tailings without compost and bacterial isolates, (2) tailings with bacterial isolates, (3) tailings with compost, and (4) tailings with compost and bacterial isolate. A descriptive analysis was carried out to analyze the role of the Pseudomnas aeruginosa RTKP1 on gold reduction in the media and gold bioaccumulation in Pteris vittata L. A quantitative analysis was carried out to analyze the bioaccumulation ability of Pteris vittata L with and without the addition of bacterial isolate. The role of the Pseudomonas aeruginosa RTKP1 is to increase the Translocation Factor (TF) and Biological Concentration Factor (BCF), particularly in the roots. The addition of compost to tailings as growing media for Pteris vittata L inhibits the effectiveness of the work of the Pseudomonas aeruginosa RTKP1 in increasing BCF and TF. However, this effect needs to be tested further to obtain significant results.
Pengaruh variasi jenis dan peletakan refugia terhadap kemelimpahan serangga pada ekosistem sawah Aminatun, Tien; Suryadarma, I Gusti Putu; Suhartini, Suhartini; Sujangka, Arsyad
Jurnal Penelitian Saintek Vol 28, No 1 (2023)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.58324

Abstract

Tujuan penelitian ini adalah mengkaji pengaruh variasi jenis dan peletakan tanaman refugia terhadap kemelimpahan serangga pada pertanaman padi dan perlakuan variasi jenis dan peletakan tanaman refugia yang paling berpotensi dalam natural pest control berdasarkan kanekaragaman musuh alami. Metode penelitian ini dilakukan dengan menanam tumbuhan kenikir (Tagetes erecta L.) dan kacang panjang (Vigna unguiculata (L.) Walp.) yang masing-masing dengan variasi peletakan di pinggir dan di tengah plot pertanaman padi. Setiap perlakuan terdiri atas 3 plot ulangan, dengan luas setiap plot 4 m2 . Pengamatan keanekaragaman dan kemelimpahan serangga dilakukan 3 minggu sekali selama 4 kali dalam satu musim tanam padi. Hasil penelitian menunjukkan variasi jenis dan peletakan tanaman refugia berpengaruh terhadap kemelimpahan serangga. Perlakuan refugia tanaman kacang panjang di pinggir plot memiliki keanekaragaman musuh alami yang lebih tinggi dibandingkan di tengah plot pertanaman padi, akan tetapi keanekaragaman musuh alami tertinggi adalah pada perlakuan refugia tanaman kenikir di tengah plot pertanaman padi. Hal ini menunjukkan bahwa perlakuan tanaman refugia kenikir di tengah pertanaman padi paling berpotensi dalam natural pest control karena memiliki indeks keanekaragaman musuh alami paling tinggi, sehingga mampu menciptakan agroekosistem yang lebih stabil.  
The Effect of Pellets and Natural Feed Combination in the Form of Insects on the Protein Content of Catfish (Clarias sp.) in the Minapadi Cultivation System Trinandita, Syfa; Aminatun, Tien; Suhartini; Putri, Rizka Apriani; Budiwati; Sujangka, Arsyad
Jurnal Sains Dasar Vol. 14 No. 2 (2025): October 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v14i2.83499

Abstract

This study aims to analyze the effect of the combination of pellets and natural feed on catfish protein levels in the minapadi system. The method used was an experimental design with two feeding treatments, which were treatment A (a combination of pellets and natural feed) and treatment B (pellets only). The natural feed consisted of insects available within the minapadi system, while the pellets used were commercial pellets. The study was conducted for 76 days in the rice field area of Donotirto Village, Kapanewon Kretek, Bantul Regency, Special Region of Yogyakarta. Catfish protein content was tested using the Kjeldahl method. The data obtained was statistically analysed using an independent t-test. The average protein content of catfish in treatment A was 16.17%, whereas that in treatment B was 16.89%. The statistical analysis indicated that the combination of pellets and natural feed had no significant effect on the protein content of catfish. These results are presumably influenced by the limited availability of insects during the study, which limited their contribution as natural feed to significantly enhance the protein content of catfish. Theese finding indicates that the utilization of insects within the minapadi system requires improved management and adjustments in cultivation practices to increase their availability. Further studies are therefore necessary to explore alternative natural feed sources as well as modifications to the minapadi system that could enhance insect availability as an additional nutritional resource for catfish.