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dwi ayu pratiwi, nabila
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Public Health

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The Effect of Boiled Mung Bean Seeds on Alanine Aminotransferase Levels in Rats (Rattus norvegicus L.) Induced by NaNO2 dwi ayu pratiwi, nabila; Mufidah, Hartalina; Imroatul Muflihah, Ahdiah; Anggitasari, Wima
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 2 No. 2 (2024): Desember
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v2i2.26

Abstract

Background: Sodium nitrite is a chemical compound used in the coloring and preserving process of processed meat. Excess sodium nitrite entering the body can have clinical impacts on health, especially the liver. Disorders of the liver can be identified by increased levels of Alanine Aminotransferase. Mung beans contain antioxidants, a compound that has benefits as a hepatoprotection. Purpose: This study aims to determine the effect of Mung bean stew on Alanine aminotransferase levels in rats (Rattus norvegicus L.) with anemia before and after administration. Method: This research design is an experimental study with quantitative data collection. This study was conducted in June–July 2024. The sample in this study was rats (Rattus norvegicus), which were taken randomly using random sampling techniques. The Mung beans used in this study were Mung beans that had undergone an infusion process. After collecting data, the researcher processed the data using Paired sample-t test analysis, One-Way ANOVA, and Post Hoc Tukey using SPSS. Results: The Paired sample-t test for Alanine Aminotransferase levels in rats (Rattus norvegicus) with anemia showed sig>0.05 in all treatment groups. The test results of the average change in Alanine Aminotransferase levels before and after between groups showed a sig value <0.05. Conclusion: Based on this study, it can be concluded that Based on this research, it can be concluded that there is no effect of boiled Mung beans on Alanine aminotransferase levels.
Effects of Various Mung Bean Decoction Doses on Hematological Profile in Anemic Wistar Rats Dwi Ayu Pratiwi, Nabila; Mufidah, Hartalina; Imroatul Muflihah, Ahdiah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.32

Abstract

Background: Anemia remains a significant global public health problem, with iron deficiency being the primary cause. Mung beans (Vigna radiata L.) are nutritionally rich plants containing iron and glycine, which are essential for hemoglobin synthesis and red blood cell formation Purpose: This study aimed to evaluate the effects of various doses of mung bean decoction on hematological profile improvement in anemic Wistar rats induced with sodium nitrite (NaNO₂). Methods: An experimental study was conducted from June to August 2024 involving 25 male Wistar rats divided into five groups: healthy control (aquadest), negative control (NaNO₂), and three treatment groups receiving NaNO₂ followed by mung bean decoction at doses of 0.2 ml, 0.4 ml, and 0.8 ml respectively. Blood samples were collected before and after treatment for hemoglobin level assessment. Data were analyzed using paired sample t-test and One-way ANOVA with post-hoc Tukey tests. Results: The One-way ANOVA test showed highly significant differences in hemoglobin level changes between groups (p < 0.0001). The 0.2 ml dose group exhibited the highest hemoglobin increase from 8.12 g/dl to 17.70 g/dl (p = 0.0002), followed by the 0.4 ml dose group with an increase from 7.32 g/dl to 16.08 g/dl (p < 0.0001), and the 0.8 ml dose group with an increase from 6.40 g/dl to 14.16 g/dl (p = 0.0005). Conclusions: Mung bean decoction effectively improved hemoglobin levels in anemic Wistar rats, with the 0.2 ml dose demonstrating the most pronounced therapeutic effect.
Co-Authors AHDIAH IMROATUL MUFLIHAH Anggitasari, Wima Imroatul Muflihah, Ahdiah Mufidah, Hartalina