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All Journal HAYATI Journal of Biosciences BIOVALENTIA: Biological Research Journal
Mutiara, Ilma
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology

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Potential Probiotic Yeasts of the Pichia Genus Isolated from ‘Dadih’, a Traditional Fermented Food of West Sumatra, Indonesia Chihombori, Tatenda Calvin; Mustopa, Apon Zaenal; Astuti, Rika Indri; Mutiara, Ilma; Refli, Redoyan; Umami, Rifqiyah Nur; Fatimah; Irawan, Herman; Ekawati, Nurlaili; Trinugroho, Joko P; Akmaliyah, Rizna; Chairunnisa, Sheila; Amani, Febriyanti Nur; Manguntungi, Baso; Hertati, Ai; Mamangkey, Jendri
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.320-340

Abstract

Fermented buffalo milk, known as dadih, serves as a reservoir of potential probiotic yeasts. Over the past two decades, probiotic yeasts have gained increasing attention in both basic and clinical sciences due to their health benefits. This study aimed to isolate and characterize probiotic yeasts from dadih. Yeasts were isolated using yeast Extract, peptone, and dextrose (YPD) medium, and molecularly identified through 18S-rRNA sequencing. Probiotic potential was assessed by evaluating resistance to acidic pH, bile salts, proteolytic, lipolytic, and hemolytic activities. Secondary metabolites produced during fermentation were tested for antimicrobial properties. GBT30 and GBT37 isolates were selected based on their superior performance in probiotic property assays for further analysis. Molecular identification revealed these isolates as Pichia occidentalis (GBT30) and Pichia kudriavzevii (GBT37). Both strains demonstrated in vitro survivability under simulated gastrointestinal conditions and exhibited antimicrobial activity. Whole-genome sequencing of P. kudriavzevii GBT37 identified a genome size of 10,906,850 base pairs, distributed across four chromosomes with a GC content of 38.26%. Notably, secondary metabolite biosynthesis genes were located on contig 7. In addition, 26 probiotic-related genes, including GSY1, HSC82, HSP104, TPS1, ARN1, FLO1, ALA1, SIR2, and others, were identified in P. kudriavzevii GBT37, indicating its potential as a probiotic yeast. The traditional fermentation process of dadih offers probiotic yeasts with promising health benefits, supporting its potential as a functional food.
Biotherapeutics and Antibiofilm Properties of Sponge-Associated Bacteria from Tanjung Tiram Mangroves, Southeast Sulawesi Mamangkey, Jendri; Sunarto, Sunarto; Fadjri, Corrina Lailatul; Irawan, Herman; Huda, Muhammad Komarul; Wibowo, Des Saputro; Mutiara, Ilma
BIOVALENTIA: Biological Research Journal Vol. 11 No. 2 (2025)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.11.2.2025.504

Abstract

 The search for new therapeutic agents has increasingly focused on bioactive compounds from sponge-associated bacteria. This study investigated bacterial symbionts of marine sponges collected from the mangrove ecosystem of Tanjung Tiram, Southeast Sulawesi, which have not been previously explored for their bioactive potential. Thirty bacterial isolates were screened in triplicate for antibacterial, antioxidant, antidiabetic, and antibiofilm activities. Antibacterial activity was evaluated using the disc diffusion method, antioxidant capacity by DPPH radical scavenging assay, α-glucosidase inhibition for antidiabetic potential, and antibiofilm activity by the crystal violet binding method. Several isolates showed notable bioactivities: strain Sp.8 produced the largest inhibition zone against Escherichia coli (15 mm), and Sp.14 showed the strongest inhibition against Staphylococcus aureus (23 mm). The most active antioxidant strains (Sp.14, Sp.15, Sp.13, and Sp.6) exhibited radical scavenging activity above 80%, while Sp.13, Sp.15, Sp.31, and Sp.37 showed high α-glucosidase inhibition (>74%). Strains Sp.38 and Sp.12 significantly reduced biofilm formation by E. coli and S. aureus, respectively. These findings reveal the untapped potential of sponge-associated marine bacteria from Tanjung Tiram as promising sources of bioactive metabolites for future drug discovery.
Co-Authors Ai Hertati, Ai Akmaliyah, Rizna Amani, Febriyanti Nur Apon Zaenal Mustopa Chairunnisa, Sheila Chihombori, Tatenda Calvin Ekawati, Nurlaili Fadjri, Corrina Lailatul Fatimah Huda, Muhammad Komarul Irawan, Herman Jendri Mamangkey Manguntungi, Baso Refli, Redoyan Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Sunarto Sunarto Trinugroho, Joko P Wibowo, Des Saputro