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All Journal HAYATI Journal of Biosciences Molekul: Jurnal Ilmiah Kimia
Nik Malek, Nik Ahmad Nizam
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Materials Science & Nanotechnology

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Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells Susanto, Hendra; Sholeh, Moch; Tri Endharti, Agustina; Nik Malek, Nik Ahmad Nizam; Amaliyah Firdaus, Sa’diyatul Rizqie; Yuda Handaya, Adeodatus; Taufiq, Ahmad
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.414-425

Abstract

Hepatocellular carcinoma (HCC) with high epidemiological report data. Pathogenesis in HCC also involves several signaling pathways. This study aims to evaluate the in vitro activity of Moringa seed NanoChitosan against Hep G2 liver cancer cells and Co-Treatment with Doxorubicin. Initially, nanoparticles were prepared by extracting Moringa seeds, formulating them into nano chitosan, and then characterizing the compounds and particle sizes. The IC50 dose was investigated using the MTT assay. Then, the IC50 dose was confirmed in more detail through immunofluorescence, betatrophin gene, several genes in the Wnt-βcatenin-CyclinD1 proliferation pathway, and the addition of the apoptotic effector Caspase-3 using RT-qPCR analysis. Each treatment used a single dose of NCH-Mosee and co-treatment or combination with 4 μg/ml doxorubicin. The IC50 dose was 994 μg/ml in single treatment and 649 μg/ml in combined treatment with Dox. Hep G2 showed a decrease in the expression level of each parameter measured with increasing single dose and combination treatment (p < 0.050). Histologically, cells shrank, betatrophin expression was inhibited, and luminescence was seen, which decreased with increasing dose. In conclusion, NCH-Mosee with dose-tracking toxicity combined with Dox can suppress the viability of Hep G2 cells.
Effect of Organic Solvents in the Preparation of Single Aged Garlic Transfersomes and Their Phytochemical Activities Lestari, Sri Rahayu; Gofur, Abdul; Rakhmawati, Yunita; Suharti, Suharti; Nik Malek, Nik Ahmad Nizam; Miasih, Dewi Sekar; Baroroh, Alif Rosyidah El; Annisa, Yuslinda
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.13310

Abstract

ABSTRACT. Single Aged Garlic (SAG), a fermented product from a single garlic, has been extensively studied for its health benefits due to its allicin content. To enhance SAG's drug delivery capabilities, this study aimed to characterize transfersome formulations containing SAG, investigating their phytochemical activities and the effects of different absolute solvents. Transfersome formulations, consisting of soy-phospholipid and either Span-60 (T1) or Tween-80 (T2), were prepared using absolute ethanol (EA) or a chloroform-methanol mixture (CM). Characterization included particle size, polydispersity index (PDI), and zeta potential. Phytochemical tests assessed antioxidant activity, total phenolic content, and total flavonoid content. Results showed that T2-CM formulations exhibited the best PDI (0.372 ± 0.022), smallest particle size (T1-CM: 84.333 ± 1.762 nm), and lowest zeta potential (T2-EA: -25.667 ± 0.666 mV). Additionally, T1-CM and T2-CM formulations demonstrated superior antioxidant, flavonoid, and phenolic content compared to T1-EA and T2-EA. Transfersomes formulated with organic solvents like absolute ethanol, methanol, and chloroform exhibit promising characteristics and can effectively protect the antioxidant compounds, flavonoids, and phenols present in SAG extracts. These solvents, known for their ability to dissolve polar and nonpolar compounds, facilitate the formation of stable, well-characterized transfersomes. These findings suggest that transfersomes prepared with chloroform-methanol mixtures are more promising for SAG delivery. Key words: Organic solvents, Phytochemicals, Single aged garlic, Transfersomes
Co-Authors Abdul Gofur Ahmad Taufiq Amaliyah Firdaus, Sa’diyatul Rizqie Annisa, Yuslinda Baroroh, Alif Rosyidah El Hendra Susanto Miasih, Dewi Sekar sholeh, moch Sri Rahayu Lestari Suharti Tri Endharti, Agustina Yuda Handaya, Adeodatus Yunita Rakhmawati