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Profiling of Single Garlic Extract Microencapsulation: Characterization, Antioxidant Activity, and Release Kinetic Lestari, Sri Rahayu; Ghofur, Abdul; Maslikah, Siti Imroatul; Sunaryono, Sunaryono; Rahma, Amalia Nur; Aisyah, Dahniar Nur; Mufidah, Ikfi Nihayatul; Rifqi, Nadiya Dini; Prastita, Nenes; Miasih, Dewi Sekar; Baroroh, Alif Rosyidah El
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.79072

Single garlic is known to have many benefits as an alternative therapy for various types of metabolic syndrome. The bioactive compounds, allicin and alliin, in garlic are unstable and easily degraded in digestion. Chitosan-alginate microencapsulation is thought to increase stability and protect active compound so its therapeutic effect is more optimal. This study aimed to characterize the microencapsulation chitosan-alginate of single garlic extract (MCA- SGE), as well as to examine the antioxidant activity and kinetic release of MCA-SGE in vitro. The research procedure includes the steps of single garlic extraction, preparation of MCA-SGE, characterization of MCA-SGE (PSA, SEM, and FTIR) as well as biological testing of MCA-SGE through antioxidant activity and kinetic release tests. PSA results showed the mean particle size of MCA-SGE was 439.0 ± 1.9 nm or 0.4 m with a polydispersity index (PDI) value of 0.579 ± 0.046 and a zeta potential value of 15.4 ± 0.3 mV. The SEM results showed that the morphology of MCA-SGE was spherical with a smooth surface and a micrometre size of 0.4 - 0.7 µm. The FTIR results describe a shift in absorption and addition of SGE functional groups after encapsulation. The results of the antioxidant activity test showed the antioxidant activity of MCA-SGE was 65%, while SGE was 55%. The results of the kinetic release showed that more allicin and alliin were released by SGE than MCA-SGE during the 4-hour kinetic release simulation. MCA-SGE has the potential to be used as a drug delivery system with controlled release.

Pelatihan Pembuatan Fermentasi Bawang Putih Tunggal (Aged Garlic) Sebagai Upaya Mencegah Tekanan Darah Tinggi dan Meningkatkan Ekonomi pada PKK RW 6 Kelurahan Jatimulyo, Kota Malang, Jawa Timur Lestari, Sri Rahayu; Maslikah, Siti Imroatul; Daniarsih, Ajeng; Witjoro, Agung; Annisa, Yuslinda; Miasih, Dewi Sekar; Baroroh, Alif Rosyidah El; Rahma, Amalia Nur; Aisyah, Dahniar Nur; Dewi, Putri Elok Septiana; Zulkifli, Muhammad Mauludi
TRI DHARMA MANDIRI: Diseminasi dan Hilirisasi Riset kepada Masyarakat (Jurnal Pengabdian kepada Masyarakat) Vol 4, No 2 (2024)
Publisher : JTRIDHARMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2024.004.02.80

Hipertensi atau tekanan darah tinggi merupakan masalah kesehatan yang signifikan di masyarakat sejalan dengan meningkatnya prevalensi penyakit di Indonesia dan global. Kondisi ini sering tidak bergejala namun dapat menyebabkan komplikasi serius seperti penyakit jantung dan strok yang memerlukan biaya pengobatan tinggi. Oleh karena itu, diperlukan solusi preventif yang efektif dan terjangkau. Salah satu pendekatan yang menjanjikan adalah penggunaan bawang putih tunggal yang difermentasi (aged garlic) yang diketahui memiliki kandungan senyawa aktif lebih tinggi setelah fermentasi seperti allicin yang efektif menurunkan tekanan darah dan meningkatkan kesehatan kardiovaskular. Pelatihan pembuatan fermentasi bawang putih tunggal yang dilakukan oleh PKK RW 6 Jatimulyo bertujuan untuk memberdayakan masyarakat dengan keterampilan ini, tidak hanya untuk meningkatkan kesehatan tetapi juga sebagai peluang ekonomi. Melalui pelatihan ini, peserta mempelajari proses fermentasi dari awal hingga akhir serta cara memasarkan produk aged garlic sebagai usaha tambahan. Berdasarkan inisiatif ini diharapkan dapat meningkatkan kesejahteraan ekonomi dan kesehatan masyarakat Jatimulyo serta menjadi model bagi daerah lain dalam menangani masalah serupa.

Characterization of SNEDDS Formulated with Single Bulb Garlic and its Anti-Inflammatory Effect on 3T3-L1 Lestari, Sri Rahayu; Sunaryono, Sunaryono; Susanto, Hendra; Rachmawati , Hidajah; Irawanto, Rony; Miasih, Dewi Sekar; El Baroroh, Alif Rosyidah; Annisa, Yuslinda; Djati, Muhammad Sasmito
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.793-804

Inflammation is an early symptom of a disease that reduces the level of health. Single-bulb garlic (Allium sativum L.) is used medicinally as a plant with a broad pharmacological effect, especially anti-inflammatory activity. Self-nanoemulsifying drug delivery systems (SNEDDS) have offered opportunities to improve drug delivery. The current study aimed to characterize SNEDDS-single bulb garlic extract (SBGE) and determine its potential as an anti-inflammatory agent in 3T3-L1 cells. SNEDDS was formulated from tween-80, glycerol, canola oil, and SBGE. The formula characterization is done using droplet size, polydispersity index, zeta potential, physical stability test, and antioxidant assay. The cytotoxicity test of SNEDDS-SBGE was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The anti-inflammatory activity was examined using 3T3-L1 cell methylglyoxal (MG) induction, and the expression of cytokines was measured using immunocytochemistry (ICC). The SNEDDS-SBGE had a nanoemulsion size of 42.30±1.39 nm, 0.6±0.03 for the polydispersion index, and -22.63±0.75 mV for the zeta potential. SNEDDS-SBGE was physically stable and had a high antioxidant level (47.579±8.017 %). SNEDDS-SBGE exhibited no toxic effect on 3T3-L1 cells. The administration of 62.5 µg/ml and 125 µg/ml SNEDDS-SBGE could inhibit (p<0.05) the expression of IL-1β after methylglyoxal induction. Thus, SNEDDS-SBGE may have potential anti-inflammatory properties.

Effect of Organic Solvents in the Preparation of Single Aged Garlic Transfersomes and Their Phytochemical Activities Lestari, Sri Rahayu; Gofur, Abdul; Rakhmawati, Yunita; Suharti, Suharti; Nik Malek, Nik Ahmad Nizam; Miasih, Dewi Sekar; Baroroh, Alif Rosyidah El; Annisa, Yuslinda
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.13310

ABSTRACT. Single Aged Garlic (SAG), a fermented product from a single garlic, has been extensively studied for its health benefits due to its allicin content. To enhance SAG's drug delivery capabilities, this study aimed to characterize transfersome formulations containing SAG, investigating their phytochemical activities and the effects of different absolute solvents. Transfersome formulations, consisting of soy-phospholipid and either Span-60 (T1) or Tween-80 (T2), were prepared using absolute ethanol (EA) or a chloroform-methanol mixture (CM). Characterization included particle size, polydispersity index (PDI), and zeta potential. Phytochemical tests assessed antioxidant activity, total phenolic content, and total flavonoid content. Results showed that T2-CM formulations exhibited the best PDI (0.372 ± 0.022), smallest particle size (T1-CM: 84.333 ± 1.762 nm), and lowest zeta potential (T2-EA: -25.667 ± 0.666 mV). Additionally, T1-CM and T2-CM formulations demonstrated superior antioxidant, flavonoid, and phenolic content compared to T1-EA and T2-EA. Transfersomes formulated with organic solvents like absolute ethanol, methanol, and chloroform exhibit promising characteristics and can effectively protect the antioxidant compounds, flavonoids, and phenols present in SAG extracts. These solvents, known for their ability to dissolve polar and nonpolar compounds, facilitate the formation of stable, well-characterized transfersomes. These findings suggest that transfersomes prepared with chloroform-methanol mixtures are more promising for SAG delivery. Key words: Organic solvents, Phytochemicals, Single aged garlic, Transfersomes

Pelatihan Pembuatan Fermentasi Bawang Putih Tunggal (Aged Garlic) Sebagai Upaya Mencegah Tekanan Darah Tinggi dan Meningkatkan Ekonomi pada PKK RW 6 Kelurahan Jatimulyo, Kota Malang, Jawa Timur Lestari, Sri Rahayu; Maslikah, Siti Imroatul; Daniarsih, Ajeng; Witjoro, Agung; Annisa, Yuslinda; Miasih, Dewi Sekar; Baroroh, Alif Rosyidah El; Rahma, Amalia Nur; Aisyah, Dahniar Nur; Dewi, Putri Elok Septiana; Zulkifli, Muhammad Mauludi
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 4 No 2 (2024)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2024.004.02.80

Hipertensi atau tekanan darah tinggi merupakan masalah kesehatan yang signifikan di masyarakat sejalan dengan meningkatnya prevalensi penyakit di Indonesia dan global. Kondisi ini sering tidak bergejala namun dapat menyebabkan komplikasi serius seperti penyakit jantung dan strok yang memerlukan biaya pengobatan tinggi. Oleh karena itu, diperlukan solusi preventif yang efektif dan terjangkau. Salah satu pendekatan yang menjanjikan adalah penggunaan bawang putih tunggal yang difermentasi (aged garlic) yang diketahui memiliki kandungan senyawa aktif lebih tinggi setelah fermentasi seperti allicin yang efektif menurunkan tekanan darah dan meningkatkan kesehatan kardiovaskular. Pelatihan pembuatan fermentasi bawang putih tunggal yang dilakukan oleh PKK RW 6 Jatimulyo bertujuan untuk memberdayakan masyarakat dengan keterampilan ini, tidak hanya untuk meningkatkan kesehatan tetapi juga sebagai peluang ekonomi. Melalui pelatihan ini, peserta mempelajari proses fermentasi dari awal hingga akhir serta cara memasarkan produk aged garlic sebagai usaha tambahan. Berdasarkan inisiatif ini diharapkan dapat meningkatkan kesejahteraan ekonomi dan kesehatan masyarakat Jatimulyo serta menjadi model bagi daerah lain dalam menangani masalah serupa.

The association of CDKNB-AS1 gene polymorphism and coronary artery calcification: A systematic review Tsuraya, Alfi; Miasih, Dewi Sekar; Ramadhani, Risa; Rohman, Mohammad Saifur; Raras, Tri Yudani Mardining
Heart Science Journal Vol. 7 No. 2 (2026): The Evolving Landscape of Heart Failure
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.hsj.2026.007.02.8

BACKGROUND: Coronary artery calcification (CAC) is a robust, heritable marker of subclinical atherosclerosis. Among genetic loci identified by genome-wide association studies (GWAS), 9p21 locus, particularly the CDKN2B-AS1 (ANRIL) region, has emerged as a key determinant of coronary artery disease (CAD). However, whether this signal generalizes to CAC across ancestries remains unresolved because the evidence is scattered and analytically heterogeneous, with certain populations underrepresented. OBJECTIVES: This systematic review was conducted to synthesize findings across studies that assessing CDKN2B-AS1 variants and CAC within populations. METHODS: We conducted a search in PubMed, BioMed Central, Wiley, ScienceDirect, and Cochrane from January 2010 to June 2025. Fourteen eligible studies were included in total. The JBI Critical Appraisal Checklist for Observational Studies was utilised to assess the risk of bias. Data were synthesised through a narrative approach. RESULTS: This systematic review integrates multi-ethnic evidence showing that rs1333049 and rs4977574 exhibit strong, reproducible associations with CAC severity in European and Admixed American populations, but weaker or absent effects in African and East Asian groups. In East Asia population, particularly Korean, rs10757272 shows the strongest influence. CONCLUSION: Overall, CDKN2B-AS1 functions as an important, ancestry-dependent in coronary calcification, emphasizing the need for fine-mapping and diverse cohort inclusion to achieve globally equitable genetic risk assessment.

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