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All Journal Biogenesis: Jurnal Ilmiah Biologi Biology, Medicine, & Natural Product Chemistry Borneo Journal of Biology Education (BJBE)
Wati, Dwi Kartika
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Other

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Analisis Aktivitas Antioksidan pada Ekstrak Jagung Muda (Zea mays L.) dengan menggunakan Metode DPPH Ma’rifah, Shofia Jannatul; Wahidah, Shinta Nuriyah; Azizah, Nur; Jamilah, Zahrotul; Wati, Dwi Kartika; Arianti, Leny Ardini; Maulidya, Sabrina Dita; Mudzakaroh, Luluk; Safitri, Dewi Astriana; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
Borneo Journal of Biology Education (BJBE) Vol 6, No 2 (2024): Oktober
Publisher : Faculty of Teacher Training and Education Biology Education Departement

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/bjbe.v6i2.5585

Abstract

Pada penelitian ini kami menggunakan jagung muda sebagai pembaharu dari penelitian sebelumnya. Jagung muda merupakan jagung yang masih tergolong mudah (belum berbiji) biasa disebut dengan jagung semi, janggel. Jagung memiliki kandungan fenolik, flavonoid dan tannin yang berguna sebagai antioksidan, yang mampu menghambat dan melindungi radikal bebas. Penelitian ini dilakukan untuk menguji aktivitas ektrak jagung muda menggunakan metode DPPH dengan pelarut etanol. Untuk mengetahui aktivitas antioksidan suatu tanaman, salah satu pengukuran yang paling umum dengan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil). Hasil antioksidan pada ekstrak jagung muda (Zea mays L.) menggunakan metode DPPH dengan spektrofotometri UV-Vis menunjukkan bahwa nilai IC50 pada jagung muda (Zea mays L.) sebesar 257,92. Sehingga dapat disimpulkan bahwa aktivitas antioksidan ekstrak jagung muda (Zea mays L.) termasuk kategori sangat lemah.
Identifikasi dan Analisis Senyawa Kimia pada Ekstrak Daun Murbei (Morus alba) Melalui Skrining Fitokimia dengan Metode Spektrofotometer Wati, Dwi Kartika; Achyar, Zhahrotun Khofifah Nur; Aziza, Nur; Mudzakaroh, Luluk; Pratiwi, Lidia Wahyu; Paramitha, Armanda Bella; Ramadhan, Majida; Nafisa, Nafisa; Mahyantika, Seftya
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.58677

Abstract

Mulberry leaves (Morus alba Linn.) are a plant whose potential has been widely studied because it contains phytochemical compounds such as flavonoids and phenols which have various health benefits. This plant belongs to the Moraceae family and has the characteristics of a woody trunk, branches and brown bark with a height of between 5 and 10 meters, has oval or heart-shaped leaves with tapered tips, and has oval-shaped fruit that is black when ripe. This research aims to analyze chemical compounds in mulberry leaf extract (Morus alba L.) using spectrophotometric methods. Phytochemical screening showed positive results for flavonoids (Shinoda and NaOH test), alkaloids (Wagner and Dragendorff test), tannins (Braymer's solution and base) test, and steroids (Salkowski test). UV-Vis spectrophotometric analysis at a wavelength of 200-800 nm shows maximum absorption at 786 nm for the 10-2 dilution sample. Analysis of total flavonoid content using standard quercetin showed concentrations of 50.06 mg/g and 52.87 mg/g for the 1000 ppm and 500 ppm samples, respectively. The extract yield was 29.086%, with a water content of 4.415% and an ash content of 0.1%. These findings indicate that mulberry leaf extract contains various bioactive compounds with potential pharmacological properties, especially flavonoids which can be useful for medicinal applications.
Antioxidant Activity Analysis Using DPPH and Total Phenol Methods of Soursop Leaf Extract (Annona muricata L.) Wati, Dwi Kartika; Amrullah, Kammal Nizam; Mukarommah, Salsabilatul; Sjakoer, Nour Athiroh Abdoes; Faisal, Faisal; Ramadhan, Majida; Nafisa, Nafisa
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.325-330

Abstract

Soursop leaves (Annona muricata L.) are known to contain various bioactive compounds with potential antioxidant properties. This study aimed to evaluate the antioxidant activity of soursop leaf extract using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method and to determine its total phenolic content using the Folin–Ciocalteu method. Antioxidant activity was assessed at extract concentrations of 500 and 1000 ppm, with quercetin used as a positive control. The results showed that the soursop leaf extract exhibited very strong antioxidant activity, with an IC50 value of 9,486 ppm. The total phenolic content of the extract was relatively high, ranging from 102,929 to 195,702 mg GAE/g extract. The strong antioxidant activity observed is likely associated with the high content of phenolic compounds, which play a crucial role in scavenging free radicals. These findings suggest that soursop leaf extract has significant potential as a natural antioxidant source for applications in the functional food and pharmaceutical industries.
Co-Authors Achyar, Zhahrotun Khofifah Nur Amrullah, Kammal Nizam Arianti, Leny Ardini Aziza, Nur Faisal Faisal Jamilah, Zahrotul Januarista, Terra Mahyantika, Seftya Maulidya, Sabrina Dita Ma’rifah, Shofia Jannatul Mudzakaroh, Luluk Mukarommah, Salsabilatul Nafisa, Nafisa Nour Athiroh Abdoes Sjakoer Nur Azizah Paramitha, Armanda Bella Pratiwi, Lidia Wahyu Rafsanjani, Ramizard Ramadhan, Majida Safitri, Dewi Astriana Wahidah, Shinta Nuriyah