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All Journal Airlangga Journal of Innovation Management Filogeni: Jurnal Mahasiswa Biologi
Ardani, Aliefia Miftakhul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology Social Sciences

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Effectiveness of Indigenous Bacteria of Salt-Washing Wastewater (Bittern) As Plastic Waste Degraders Saputri, Wahyu Dwi; Ardani, Aliefia Miftakhul; Junaedi, Abdus Salam; Triajie, Haryo
Airlangga Journal of Innovation Management Vol. 6 No. 1 (2025): Airlangga Journal of Innovation Management
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ajim.v6i1.65188

Abstract

Plastic is a man-made synthetic polymer that has stable and durable characteristics The process of decomposing plastic takes a long time and the use of plastic which is increasing every year results in the accumulation of plastic waste in the environment, causing environmental pollution. Biodegradation is one solution to reduce the amount of plastic with the help of microorganisms or bacteria. This study has an objective to analyze the OD value; TPC; and the percentage value of plastic waste weight loss from each Indigenous bittern bacterial isolate in degrading plastic waste on YEB media + 1% glucose. The research procedure includes sterilization of tools and materials until data analysis. Data on TPC and OD values during the degradation process were analyzed descriptively and the percentage value of plastic dry weight after 7 days of incubation time was analyzed statistically. The highest OD value was 1,613 (BB1), the highest TPC value was 13,93 Log CFU/ml (BB4), and the highest plastic dry weight percentage value was 25%+0b (BB4). This research provides new insights into the potential of Indigenous bittern bacteria as plastic waste biodegradation agents and enriches knowledge in the field of environmental biotechnology. This research can be used as a basis for the waste management industry and government to design more sustainable policies in waste management to reduce dependence on conventional methods that are expensive and less effective
Analisis Cemaran E.Coli Pada Ikan Gulamah (Jhonius. sp) Di Perairan Selat Madura, Jawa Timur Ardani, Aliefia Miftakhul; Safitri, Talitha Aulia Nadifah; Silvia, Farah Faiqotus; Felissa, Gita Shafa; Hidayat, Firman; Nexvia, Cinta Marshanda; Roushan, Muhammad Evik; Alfarizi, Rois; Sigalingging, Fery Kurniawan; Saputri, Wahyu Dwi; Junaedi, Abdus Salam
Filogeni: Jurnal Mahasiswa Biologi Vol 5 No 1 (2025): Januari-April
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v5i1.56374

Abstract

Perikanan berperan penting dalam perekonomian Jawa Timur, salah satunya melalui penangkapan ikan gulamah (Johnius sp.) di Selat Madura. Namun, pencemaran limbah domestik berpotensi mengontaminasi ikan oleh mikroba patogen seperti Escherichia coli yang berbahaya bagi kesehatan manusia. Penelitian ini bertujuan untuk mendeteksi keberadaan dan kadar cemaran E. coli pada ikan gulamah di Perairan Selat Madura. Metode yang digunakan adalah deskriptif kuantitatif. Sampel ikan yang diambil diuji melalui uji penduga coliform, uji penegasan coliform, uji penduga E. coli, dan uji penegasan E. coli. Hasil menunjukkan adanya kontaminasi E. coli dengan nilai 43 MPN/g atau 4 sel/10 ml, melebihi ambang batas ≤3 MPN/g sesuai SNI 2332.1:2015. Uji penegasan pada media EMBA menunjukkan warna hijau metalik, menandakan hasil positif E. coli. Keberadaan E. coli yang melebihi ambang batas dalam ikan gulamah dapat berisiko terhadap keamanan pangan dan kesehatan masyarakat, sehingga diperlukan upaya mitigasi pencemaran serta pengawasan ketat dalam rantai pasok perikanan di Selat Madura.
Analisis Kelimpahan Total Bakteri Coliform di Pelabuhan Kamal, Kecamatan Kamal, Kabupaten Bangkalan Saputri, Wahyu Dwi; Salma, Kholidiyah Sindy; Sintasari, Riski; Simanjutak, Sania; Romadhon, Gilang; Ariel, Maulana Habib Putra; Wahyuningtyas, Putri; Edelweis, Dinda Shafa Adzania; Dhiyaulhaq, Khaizuran Maziz; Ardani, Aliefia Miftakhul; Junaedi, Abdus Salam
Filogeni: Jurnal Mahasiswa Biologi Vol 5 No 1 (2025): Januari-April
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v5i1.56376

Abstract

Pelabuhan Kamal merupakan salah satu gerbang utama menuju Pulau Madura setelah adanya Jembatan Suramadu. Kondisi perairan Pelabuhan Kamal mengalami penurunan kualitas air yang dipengaruhi oleh aktivitas tambat-labuh kapal serta aktivitas masyarakat sekitar. Bakteri coliform menjadi bakteri indikator timbulnya pencemaran perairan yang termasuk dalam bakteri Gram negatif dan tidak membentuk spora. Tujuan dari penelitian ini adalah untuk mengetahui jumlah bakteri coliform yang ditemukan pada sampel air sekitar Pelabuhan Kamal serta melakukan pengukuran parameter kualitas air suhu, DO, pH dan salinitas. Metode pengambilan sampel menggunakan random sampling pada 3 stasiun dengan 3 kali pengulangan. Analisa laboratorium melalui identifikasi bakteri coliform menggunakan media Lactose Broth sebagai uji pendugaan serta media Brilliant Green Lactose Broth (BGLB) sebagai uji penegasan. Hasil uji parameter kualitas air (suhu, pH, DO, dan salinitas) di Perairan Pelabuhan Kamal dari semua sampel yang diperoleh masih memenuhi standar baku mutu berdasarkan Keputusan Menteri Lingkungan Hidup Nomor 51 tahun 2004. Sedangkan hasil uji MPN menunjukkan bahwa kelimpahan bakteri coliform telah melebihi standar baku mutu yang dipersyaratkan. Tingginya jumlah total bakteri coliform di perairan Pelabuhan Kamal menunjukkan adanya potensi risiko terhadap kualitas lingkungan perairan. Temuan ini menegaskan perlunya pengelolaan dan pemantauan lebih lanjut guna menjaga ekosistem dan memastikan keberlanjutan sumber daya air di kawasan tersebut.
Application of Indigenous Bacterial Formulation of Salt-Washing Wastewater (Bittern) in Degrading Plastic Waste Ardani, Aliefia Miftakhul; Saputri, Wahyu Dwi; Junaedi, Abdus Salam; Triajie, Haryo
Airlangga Journal of Innovation Management Vol. 5 No. 4 (2024): Airlangga Journal of Innovation Management
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ajim.v5i4.65189

Abstract

The accumulation of plastic waste can lead to environmental changes. One solution to reduce plastic waste is through a biodegradation process involving microorganisms or bacteria. One solution is biodegradation by using microorganisms or bacteria to break down plastic. Biodegradation is a solution to reduce plastic numbers with the help of microorganisms or bacteria. This study aims to measure the Optical Density (OD), Total Plate Count (TPC), and degradation effectiveness of indigenous bacteria from salt wash wastewater (bittern) in degrading plastic on Nutrient Broth (NB) media with 1% glucose. The research process included sterilization of tools and materials, sampling, media preparation, rejuvenation of bacterial cultures, inoculation, OD measurement, TPC calculation, and percentage of plastic weight loss. Data on OD and TPC were analyzed quantitatively with visual aids. The plastic degradation percentage was measured after 7 days of incubation. The percentage of plastic degradation was measured after 7 days of incubation. The results showed that the highest OD value was bacteria BB8 which amounted to 1.614. TPC for plastic degradation was highest in BB9 which amounted to 13.37 Log CFU/g. BB4 showed the highest percentage of plastic dry weight reduction 36.4% after 7 days. These findings highlight the potential of salt-washing wastewater bacteria for plastic waste degradation.
Co-Authors Abdus Salam Junaedi Alfarizi, Rois Ariel, Maulana Habib Putra Dhiyaulhaq, Khaizuran Maziz Edelweis, Dinda Shafa Adzania Felissa, Gita Shafa Firman Hidayat Gilang Romadhon Haryo Triajie, Haryo Junaedi, Abdus Salam Nexvia, Cinta Marshanda Putri Wahyuningtyas, Putri Roushan, Muhammad Evik Safitri, Talitha Aulia Nadifah Salma, Kholidiyah Sindy Saputri, Wahyu Dwi Sigalingging, Fery Kurniawan Silvia, Farah Faiqotus Simanjutak, Sania Sintasari, Riski