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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Unram Journal of Community Service (UJCS) Rumphius Pattimura Biological Journal Innovation for Community Service Journal Kalwedo Sains (KASA)
Ukratalo, Abdul Mahid
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Physics Social Sciences Other

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EFFECTIVENESS OF Sargassum sp EXTRACT IN REDUCE BLOOD SUGAR LEVELS AND ACCELERATE WOUND HEALING ON THE SKIN OF DIABETES MELLITUS MICE (Rattus novergicus) Kaihena, Martha; Umagapi, Muhammad Cesar; Kaliky, Arief Rahman Shabri; Tarangi, Filep Marfil; Ukratalo, Abdul Mahid
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p093-100

Abstract

Diabetes Mellitus is a metabolic disorder characterised by elevated blood sugar levels beyond normal ranges, resulting from reduced secretion of the hormone insulin by pancreatic β cells and/or impaired insulin action. Diabetes therapy is often overseen by regulating blood glucose levels regularly and preventing or reducing the risk of complications. Sargassum sp is a variety of brown seaweed native to Indonesia with possible antioxidant and antidiabetic properties. This study seeks to evaluate the efficacy of Sargassum sp extract in lowering blood sugar levels and speeding up wound healing on the skin of diabetic mice. This is an experimental study that uses mice as experimental subjects. The mice were categorised into 5 groups (K-, K+, P1, P2, and P3). Their initial blood sugar levels were recorded, a skin incision was performed, and STZ was administered. When blood sugar levels rise, the K+ group receives metformin; on the other hand, P1, P2, and P3 are administered Sargassum sp extract at a specified dosage. The data were examined using ANOVA and SPSS. The analysis revealed that the water content of Sargassum sp was 4.32%, and the yield value of the concentrated extract was 8.75%. The ethanol extract of Sargassum sp has been revealed to lower blood sugar levels and speed up the wound healing process in mice with diabetes mellitus. The effects observed include decreased blood sugar levels and enhanced wound healing percentage, which are dose-dependent.
BIOLARVACIDE ACTIVITY OF ETHANOL EXTRACT OF KEDONDONG STEM (Spondias pinnata) AGAINST Aedes aegypti Moniharapon, Debby D; Ukratalo, Abdul Mahid; Wisnanda, Bayu
RUMPHIUS Vol 1 No 1 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i1p012-017

Abstract

Aedes aegypti mosquito is a vector for Dengue Hemorrhagic Fever. One effort to prevent the spread of the disease is vector control. The use of insecticides is a form of vector control in an effort to control DHF. There are two broad categories of insecticides that are often used as household insecticides, namely insecticides that function to kill insects and insecticides that function to repel insects. Kedondong (Spondias pinnata) which is a family of anacardiaceae which generally grows well in tropical climates. Kedondong bark contains saponins, alkaloids, and flavonoids which are secondary metabolites involved in defense mechanisms against attack by many microorganisms. This study aims to determine the biolarvicidal activity of the ethanol extract of kedondong stem bark in killing Aedes aegypti mosquito larvae. This study used 5 treatments and 3 replications. Each treatment contained 20 Aedes aegypti mosquito larvae. Observations were made for 24 hours with observation times of 0 hours, 6 hours, 12 hours, 18 hours and 24 hours. The time calculation starts after the larvae are inserted into the experimental bottle. The results showed that kedondong bark extract was able to kill Aedes aegypti mosquito larvae. The effective dose of ethanol extract of kedondong stem bark in killing Aedes aegypti mosquito larvae is 20 mg/mL.
Pelatihan Pengamatan Sel Dengan Mikroskop Olympus Cx-21 dan Dokumentasi Sel Menggunakan Smartphone Bagi Siswa Dan Guru Ma Al-Muluuk Persis Telaga Kodok, Leihitu, Kabupaten Maluku Tengah Seumahu, Cecilia Anna; Leimena, Handy Erwin Pier; Sahertian, Dece Elisabeth; Ukratalo, Abdul Mahid
Innovation for Community Service Journal Vol 2 No 1 (2024): April 2024
Publisher : Department of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/icsj.v2i1.12486

Abstract

Penggunaan mikroskop sebagai alat dalam praktikum biologi menjadi sangat penting karena dapat mendukung keterampilan proses belajar sains. Tujuan dari kegiatan ini adalah untuk memberikan pemahaman yang mendalam kepada siswa dan guru mengenai penggunaan mikroskop Olympus CX-21 untuk pengamatan sel serta penggunaan smartphone untuk dokumentasi sel. Metode yang digunakan adalah ceramah, diskusi, tanya jawab serta demonstrasi untuk menyiapkan preparat segar tumbuhan, menggunakan mikroskop dengan benar serta mendokumentasi hasil pengamatan. Hasil yang dicapai adalah peserta mampu menggunakan mikroskop dan menyiapkan preparat segar tumbuhan dan mendokumentasikan serta memvisualisasikan hasil pengamatan. Peserta terlihat sangat antusias dan tertarik karena mampu mengamati jaringan tumbuhan langsung di bawah mikroskop.
Introducing Indonesian endemism, its threat and conservation solution for SMA Negeri 10 students in Kamarian Village, West Seram Regency, Maluku Leimena, Handy Erwin Pier; Seumahu, Cecilia Anna; Sahertian, Dece Elizabeth; Ukratalo, Abdul Mahid; Kaihena, Martha
Unram Journal of Community Service Vol. 5 No. 1 (2024): March
Publisher : Pascasarjana Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/ujcs.v5i1.585

Abstract

West Seram Regency on Seram Island in Maluku Province, Indonesia has extraordinary biodiversity and is the habitat of several endemic species of Maluku and Indonesia. As a form of implementation of the dharma of community service, socialization was conducted with students of SMA Negeri 10 in Kamarian Village, West Seram Regency, Maluku about Indonesian endemism, its threats and conservation. The socialization was attended by 38 students. The socialization results indicate that 91% of students demonstrated a good understanding of endemic species, and 82% of students obtained information on endemic species from this activity. Additionally, students were able to identify 11 Indonesian endemic species, as well as the threats and efforts to protect them. This activity has the potential to increase environmental awareness among school students, the nation's young generation.
Bioprospeksi Ekstrak Etanol Gagang Cengkeh (Syzogium aromatica) Sebagai Biolarvasida Nyamuk Aedes aegypti Ayal, Yonri; Nindatu, Maria; Ukratalo, Abdul Mahid
Kalwedo Sains (KASA) Vol 2 No 1 (2021): Kalwedo Sains (KASA), Maret 2021
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav2i1p7-14

Abstract

Vektor penyebab penyakit DBD adalah nyamuk Aedes aegypti yang dapat ditemukan hampir di seluruh provinsi di Indonesia karena nyamuk ini sangat mudah beradaptasi dengan lingkungan sekitarnya. Cengkeh (Syzogium aromatica) merupakan tanaman rempah asli Kepulauan Maluku. Masyarakat Maluku sering menggunakan gagang cengkeh dalam upaya mengusir nyamuk. Penggunaan gagang cengkeh dalam mengusir nyamuk umumnya dengan cara dibakar. Asap hasil pembakaran gagang cengkeh tersebut efektif dalam membasmi nyamuk. Tujuan dari penelitian ini adalah membuktikan ekstraketanol gagang cengkeh (Syzogium aromatica) dapat membunuh larva nyamuk Aedes aegypti. Penelitian menggunakan 5 perlakuan dan 3 kali ulangan. Tiga ratus larva nyamuk instar III dimasukan pada tiap kelompok perlakuan. Tiap wadah diisi 20 larva. Pengamatan aktivitas larvasida dilakukan setiap 4 jam sekali selama 24 jam. Data hasil pengamaan dianalisis menggunakan ANOVA pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa ekstrak etanol gagang cengkeh dapat membunuh larva nyamuk Aedes aegypti.Nilai LC50 ekstrak etanol gagang cengkeh sebesar 2,386 dengan batas bawah 0,319 dan batas atas 3,836.
Analisis Kandungan Gizi Dan Jumlah Mikroba Produk Ikan Kaleng Yang Dijual Di Beberapa Supermarket Di Kota Ambon Berdasarkan Lama Penyimpanan Kaihena, Martha; Ukratalo, Abdul Mahid
Kalwedo Sains (KASA) Vol 3 No 1 (2022): Kalwedo Sains (KASA), Maret 2022
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav3i1p55-62

Abstract

Ikan kaleng merupakan salah satu jenis makanan yang cukup digemari karena praktis dan dapat disimpan dalam jangka waktu lama. Namun, komposisi kaleng yang terbuat dari logam berpotensi mencemari makanan sehingga berpotensi terdapat logam berat dan mikroba dalam produk ikan kaleng tersebut. Penelitian ini bertujuan untuk mengetahui kandungan gizi dan jumlah mikroba pada produk ikan kaleng yang dijual pada beberapa supermarket di Kota Ambon. Sampel ikan kaleng dibeli dari beberapa supermarket di Kota Ambon. Ikan kaleng yang digunakan adalah ikan kaleng jenis A, B dan C. Ikan kaleng yang telah dibeli dari supermarket kemudian di bawah ke Laboratorium Kimia Dasar untuk proses lebih lanjut. Ikan kaleng disimpan dengan lama penyimpanan 7, 14 dan 21 hari. Hasil penelitian menunjukkan bahwa penyimpanan produk ikan kaleng selama 7, 14 dan 21 hari menyebabkan peningkatan kadar protein dan kadar abu, sedangkan kadar air dan kadar lemak mengalami penurunan. Hasil uji terhadap kandungan bakteri pada produk ikan kaleng yang disimpan selama 7, 14 maupun 21 hari tidak menunjukkan adanya pertumbuhan mikroba.
Methanol Extract Alstonia scholaris L. R. Br as Hepatoprotective Mice (Mus musculus) Infected with Plasmodium berghei ANKA Strains Kaihena, Martha; Nindatu, Maria; Ukratalo, Abdul Mahid
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.4834

Abstract

Alstonia scholaris L. R. Br is one of the traditional plants that contain natural antioxidant compounds which are thought to be able to repair damage to the liver cells of mice (Mus musculus) infected with Plasmodium berghei strain ANKA. This study aimed to determine the effect of methanol extract of Alstonia scholaris L. R. Br stem bark on levels of SGPT enzymes and liver cells of mice (Mus musculus) infected with Plasmodium berghei strain ANKA. Mice with a body weight of 20-30 g were infected with Plasmodium berghei as much as 0.1 ml per head and left until the percentage of parasitemia reached 1-5%. Then mice (Mus musculus) were given methanol extract of Alstonia scholaris L. R. Br stem bark at doses of 1, 10, 100, and 200 mg/kg BW for 4 consecutive days. After that, surgery was performed to take blood to observe SGPT enzyme levels and mice liver cells to be prepared with Hematoxylin Eosin (HE) staining. The results of ANOVA showed that the methanol extract of Alstonia scholaris L. R. Br stems bark doses of 1 mg/kg BW, 10 mg/kg BW, 100 mg/kg BW and 200 mg/kg BW could reduce SGPT enzyme levels and repair damage to the liver cells of mice infected with Plasmodium berghei ANKA strains
The Immunomodulatory Activity of Cinnamomum burmanni Bark Extract on Leucocyte Differentiation of Mice (Mus musculus) In Diabetes Mellitus Model Kaihena, Martha; Ukratalo, Abdul Mahid; Killay, Amos; Kaliky, Nunun Ainun Putri Sari Banun
Jurnal Penelitian Pendidikan IPA Vol 10 No 1 (2024): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i1.5947

Abstract

Diabetes mellitus frequently coexists with infection, leading to an immunological response characterised by an elevation in leukocyte levels. This research aimed to assess the immunomodulatory activity of an extract derived from the bark of Cinnamomum burmanni on the differentiation of leukocytes in a diabetes mellitus model using mice (Mus musculus). Prior to the administration of therapy, the glucose levels of all mice were assessed. Subsequently, groups K (+), P1, P2, and P3 were administered STZ at 0.2 ml per individual. The blood glucose levels (post-STZ blood glucose) were checked once more on the seventh day. In the event of a rise in blood glucose levels over (>200 mg/dL), subjects belonging to groups P1, P2, and P3 were administered Cinnamomum burmanii extract under the prescribed dosage. The intervention was administered for 14 days, and blood glucose levels were assessed on the final day of experimentation. On the final day of the experiment, blood samples were collected from the mice's tails in order to prepare blood preparations. The findings indicate that the methanol extract derived from cinnamon bark possesses immunomodulatory properties, as evidenced by its ability to decrease the population of eosinophils, lymphocytes, and monocytes while concurrently increasing the amount of neutrophils.
Co-Authors Amos Killay Ayal, Yonri CECILIA ANNA SEUMAHU Handy Erwin Pier Leimena Kaliky, Arief Rahman Shabri Kaliky, Nunun Ainun Putri Sari Banun Martha Kaihena Moniharapon, Debby D Nindatu, Maria Sahertian, Dece Elisabeth Sahertian, Dece Elizabeth Tarangi, Filep Marfil Umagapi, Muhammad Cesar Wisnanda, Bayu