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All Journal Rumphius Pattimura Biological Journal Innovation for Community Service Journal
Ukratalo, Abdul Mahid
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Social Sciences Other

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EFFECTIVENESS OF Sargassum sp EXTRACT IN REDUCE BLOOD SUGAR LEVELS AND ACCELERATE WOUND HEALING ON THE SKIN OF DIABETES MELLITUS MICE (Rattus novergicus) Kaihena, Martha; Umagapi, Muhammad Cesar; Kaliky, Arief Rahman Shabri; Tarangi, Filep Marfil; Ukratalo, Abdul Mahid
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p093-100

Abstract

Diabetes Mellitus is a metabolic disorder characterised by elevated blood sugar levels beyond normal ranges, resulting from reduced secretion of the hormone insulin by pancreatic β cells and/or impaired insulin action. Diabetes therapy is often overseen by regulating blood glucose levels regularly and preventing or reducing the risk of complications. Sargassum sp is a variety of brown seaweed native to Indonesia with possible antioxidant and antidiabetic properties. This study seeks to evaluate the efficacy of Sargassum sp extract in lowering blood sugar levels and speeding up wound healing on the skin of diabetic mice. This is an experimental study that uses mice as experimental subjects. The mice were categorised into 5 groups (K-, K+, P1, P2, and P3). Their initial blood sugar levels were recorded, a skin incision was performed, and STZ was administered. When blood sugar levels rise, the K+ group receives metformin; on the other hand, P1, P2, and P3 are administered Sargassum sp extract at a specified dosage. The data were examined using ANOVA and SPSS. The analysis revealed that the water content of Sargassum sp was 4.32%, and the yield value of the concentrated extract was 8.75%. The ethanol extract of Sargassum sp has been revealed to lower blood sugar levels and speed up the wound healing process in mice with diabetes mellitus. The effects observed include decreased blood sugar levels and enhanced wound healing percentage, which are dose-dependent.
BIOLARVACIDE ACTIVITY OF ETHANOL EXTRACT OF KEDONDONG STEM (Spondias pinnata) AGAINST Aedes aegypti Moniharapon, Debby D; Ukratalo, Abdul Mahid; Wisnanda, Bayu
RUMPHIUS Vol 1 No 1 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i1p012-017

Abstract

Aedes aegypti mosquito is a vector for Dengue Hemorrhagic Fever. One effort to prevent the spread of the disease is vector control. The use of insecticides is a form of vector control in an effort to control DHF. There are two broad categories of insecticides that are often used as household insecticides, namely insecticides that function to kill insects and insecticides that function to repel insects. Kedondong (Spondias pinnata) which is a family of anacardiaceae which generally grows well in tropical climates. Kedondong bark contains saponins, alkaloids, and flavonoids which are secondary metabolites involved in defense mechanisms against attack by many microorganisms. This study aims to determine the biolarvicidal activity of the ethanol extract of kedondong stem bark in killing Aedes aegypti mosquito larvae. This study used 5 treatments and 3 replications. Each treatment contained 20 Aedes aegypti mosquito larvae. Observations were made for 24 hours with observation times of 0 hours, 6 hours, 12 hours, 18 hours and 24 hours. The time calculation starts after the larvae are inserted into the experimental bottle. The results showed that kedondong bark extract was able to kill Aedes aegypti mosquito larvae. The effective dose of ethanol extract of kedondong stem bark in killing Aedes aegypti mosquito larvae is 20 mg/mL.
Pelatihan Pengamatan Sel Dengan Mikroskop Olympus Cx-21 dan Dokumentasi Sel Menggunakan Smartphone Bagi Siswa Dan Guru Ma Al-Muluuk Persis Telaga Kodok, Leihitu, Kabupaten Maluku Tengah Seumahu, Cecilia Anna; Leimena, Handy Erwin Pier; Sahertian, Dece Elisabeth; Ukratalo, Abdul Mahid
Innovation for Community Service Journal Vol 2 No 1 (2024): April 2024
Publisher : Department of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/icsj.v2i1.12486

Abstract

Penggunaan mikroskop sebagai alat dalam praktikum biologi menjadi sangat penting karena dapat mendukung keterampilan proses belajar sains. Tujuan dari kegiatan ini adalah untuk memberikan pemahaman yang mendalam kepada siswa dan guru mengenai penggunaan mikroskop Olympus CX-21 untuk pengamatan sel serta penggunaan smartphone untuk dokumentasi sel. Metode yang digunakan adalah ceramah, diskusi, tanya jawab serta demonstrasi untuk menyiapkan preparat segar tumbuhan, menggunakan mikroskop dengan benar serta mendokumentasi hasil pengamatan. Hasil yang dicapai adalah peserta mampu menggunakan mikroskop dan menyiapkan preparat segar tumbuhan dan mendokumentasikan serta memvisualisasikan hasil pengamatan. Peserta terlihat sangat antusias dan tertarik karena mampu mengamati jaringan tumbuhan langsung di bawah mikroskop.
Co-Authors CECILIA ANNA SEUMAHU Handy Erwin Pier Leimena Kaliky, Arief Rahman Shabri Martha Kaihena Moniharapon, Debby D Sahertian, Dece Elisabeth Tarangi, Filep Marfil Umagapi, Muhammad Cesar Wisnanda, Bayu