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All Journal BULETIN OSEANOGRAFI MARINA Jurnal Kelautan Tropis
Doktorasaintifika, Heradita Kaniaazzahra
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Earth & Planetary Sciences

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Molecular Characterization of Nannochloropsis sp. Based on tufA Genetic Marker and Potential Test of Nannochloropsis sp. as a Cadmium (Cd) Heavy Metal Bioremediation Agent Setyowati, Sulis; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad; Suprihadi, Agung; Doktorasaintifika, Heradita Kaniaazzahra; Herida, Azalia Puspa; Wahyuningsih, Candra; Jannah, Siti Nur; Wijanarka, Wijanarka
Buletin Oseanografi Marina Vol 14, No 1 (2025): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v14i1.66422

Abstract

Cadmium (Cd) heavy metal pollution not only affects aquatic ecosystems but also has toxic effects on human health. Bioremediation using microalgae Nannochloropsis sp. is considered more economical and sustainable for overcoming heavy metal pollution. This study aims to molecular characterization of Nannochloropsis sp. with tufA gene markers, determine the effect of different Cd concentrations on the growth and morphology of Nannochloropsis sp. and the ability of Nannochloropsis sp. to absorb Cd concentrations. The methods used include DNA isolation, quantitative and qualitative DNA analysis, amplification of tufA gene, sequencing result analysis, phylogenetic tree analysis, and bioremediation test. The results of molecular characterization showed that Nannochloropsis sp. has similarities with N. oceanica strain BR2 plastid (CP044614.1) with 60% bootstrap value. Nannochloropsis sp. grew at different Cd concentration (0, 2, 4, and 6 ppm) but optimal at 0 ppm Cd concentration. Morphology of Nannochloropsis sp. showed morphological differences in the cell structure. The cell was broken and the color turned increasingly clear. Different concentrations of Cd metal on Nannochloropsis sp. growth significantly affected the decline in Nannochloropsis sp. growth. Nannochloropsis sp. has the potential to absorb heavy metal Cd with an efficiency that increases as the concentration reaching 62.6% at 6 ppm. It can be concluded that molecular characterization using the tufA marker was effective in showing that Nannochloropsis sp. had closed relation with N. oceanica strain BR2 plastids. Cadmium (Cd) exposure significantly reduced growth and caused cell damage, while Nannochloropsis sp. effectively adsorbed Cd and lowering Cd levels by 3.76 ppm.
Molecular Characterization of Microalgae Thalassiosira sp. Based on Genetic Marker tufA and Potential Test for Bioremediation of Heavy Metal Lead (Pb) Izzudin, Maulana Zidan; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad; Nurhayati, Nurhayati; Jannah, Siti Nur; Wahyuningsih, Candra; Herida, Azalia Puspa; Doktorasaintifika, Heradita Kaniaazzahra
Jurnal Kelautan Tropis Vol 28, No 1 (2025): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v28i1.25463

Abstract

Heavy metal lead (Pb) pollution in aquatic environments poses a serious threat to aquatic ecosystems and human health. The microalga Thalassiosira  sp. has potential as a bioremediation agent for heavy metals; however, further studies are needed to maximize its capability. This study aims to molecularly characterize Thalassiosira  sp. using the tufA gene, evaluate the effects of Pb on growth and cell morphology, and assess its Pb uptake efficiency. Methods included DNA isolation, phylogenetic analysis, growth measurement using a hemocytometer, Pb uptake testing via ICP-OES, and cell morphology analysis. Results showed that the tufA gene could molecularly characterize Thalassiosira  sp., with a phylogenetic similarity of 99.53% to Conticriba weissflogii (MH571875.1). Pb significantly inhibited growth at concentrations of 0.03 ppm and 0.05 ppm and caused cell aggregation and pigment degradation at higher concentrations (2 ppm). This microalga exhibited the highest Pb uptake efficiency at lower concentrations (0.03–0.05 ppm), reaching 100%, while at 2 ppm, the efficiency decreased to 40%. This study highlights the potential of Thalassiosira  sp. in remediating Pb, providing a foundation for the development of microalgae-based biotechnological applications.
Co-Authors Chatarina Umbul Wahyuni Herida, Azalia Puspa Hermin Pancasakti Kusumaningrum Izzudin, Maulana Zidan Muhammad Zainuri Nurhayati Nurhayati Siti Nur Jannah Sulis Setyowati, Sulis Wahyuningsih, Candra Wijanarka Wijanarka