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All Journal JURNAL ILMIAH PLATAX Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Riset Rumpun Ilmu Hewani (JURRIH) Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Nistiarni Zebua
Universitas Nias
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Social Sciences Veterinary

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Journal : JURNAL ILMIAH PLATAX

 1 
Study of e-DNA Quality at Fishing Ground of Manado Bay, North Sulawesi Province. Zebua, Nistiarni; Mandagi, Ixchel F.; Masengi, K.W.A; Luasunaung, Alfret; Rumengan, Inneke F. M.; Wulur, Stenly; Makapedua, Daisy M.; Masengi, E. I. K.G.; Sumilat, Deiske A.; Masengi, Akira W. R.; Manoppo, Victoria
Jurnal Ilmiah Platax Vol. 13 No. 1 (2025): ISSUE JANUARY-JUNE 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i1.57404

Abstract

We used the Nansen Bottle Sampler to collect water samples in the deepsea area, ranging from 150 meters to 175 meters in six water points around Manado Bay, to test the quality of e-DNA water samples to detect target species in the fishing area. Therefore, the basis of the case study method with a sampling technique was carried out on July 29 2023 using Power Water Sterivex Kits, water samples were then stored at -250C and were then taken to TBRC, University of the Ryukyus for further laboratory works, such as; eDNA extract, eDNA quality testing, 1st and 2nd PCR and Electrophoresis eDNA analysis processes using MiFish-U primers with a target of 163–185bp and 375 bp, following the MiFish protocol.  Based on the results of the eDNA extract solution, it is known that the quality of eDNA from the 6 sampling sites locations ranged between 2.8 µg/mL – 4.4 µ g/mL, which means a good quality of eDNA. Moreover, it showed that the presence of DNA fragments at Kappa 60◦C Gelelectrophoresis 1st-PCR, 12S rRNA gene product (163–185bp), and Kappa 60◦C and 65◦C Geklelectrophoresis 2nd-PCR Products according to the target amplicon 375 bp. This means we can conduct the next step, the PCR sequence analysis.  Then, eDNA quality testing, 1st and 2nd PCR, and Electrophoresis of e-DNA analysis process were using MiFish-U F/R primers with a target of 375 bp, it is known that the concentration of Nanodrop from the 6 sampling locations ranges between 2.8 µg/mL – 4.4 µg/mL while the core or quality eDNA ranged from 1.56 µg/mL – 2.50 µg/mL. Based on identification results, five types of species were detected; Myctophum lychnobium, Selar crumenophthalmus, Photonectes sp., Oreochromis sp. Thunnus obesus and Homo sapiens were generated using eDNA metabarcoding on the mitochondria genome database MitoFish. Keywords: e-DNA, eDNA quality, species target, fishing area, Manado Bay   Abstrak Kami menggunakan Nansen Bottle Sampler untuk mengambil sampel air pada laut dalam berkisar 150meter sampai 175 meter di enam titik perairan Sekitar Teluk Manado, untuk menguji Kualitas e-DNA sample air yang digunakan untuk mendeteksi target spesies pada daerah penangkapan. Selanjutnya dasar metode studi kasus dengan teknik pengambilan sampel secara sampling dilakukan pada tanggal 29 Juli 2023 menggunakan Power Water Sterivex Kits, sapel air disimpan pada -250C yang selanjutnya dibawa ke TBRC, University of the Ryukyus untuk pnelitian laboratorium lanjutan seperti ekstrak eDNA, Pengujian kualitas eDNA, 1st and 2nd PCR dan Elektrophoresis proses analisis eDNA menggunakan primer MiFish-U dengan target 375 bp, mengikuti MiFish protokol. Berdasarkan hasil pengujian larutan ekstrak eDNA diketahui bahwa kualitas eDNA dari 6 titik lokasi sampling berkisar antara 2.8 ng/mL – 4.4 ng/mL dan menunjukkan adanya fragment DNA pada Kappa 60◦C Geklelectrophoresis 1st-PCR Produk 12S rRNA gene (163–185bp, dan Kappa 60◦C dan 65◦C Geklelectrophoresis 2nd-PCR Produk sesuai amplikon target 375 bp. Hal ini berarti dapat dilanjutkan pada tahap analisis sekuens PCR. Pengujian kualitas eDNA, 1st and 2nd PCR dan Elektrophoresis proses analisis eDNA menggunakan primer MiFish-U F/R dengan target 375 bp, diketahui bahwa konsentrasi Nanodrop dari 6 titik lokasi sampling berkisar antara 2.8 µg/mL – 4.4 µg/mL sedangkan kemurnian atau kualitas eDNA berkisar antara 1.56 µg/mL – 2.50 µg/mL. Hasil identifikasi menyatakan lima jenis spesies terdeteksi; Myctophum lychnobium, Selar crumenophthalmus, Photonectes sp., Oreochromis sp. Thunnus obesus, Homo sapiens dihasilkan dengan menggunakan eDNA metabarcoding pada MitoFish database genom mitokondria. Kata kunci : e- DNA, kualitas eDNA, target spesies, daerah penangkapan, Teluk Manado.
Analysis of the Role of Seagrass Biodiversity in Supporting the Conservation of Marine Ecosystems in the Nias Islands, North Sumatra Zebua, Nistiarni; Zebua, Ratna Dewi; Telaumbanua, Betzy V.; Laoli, Destriman; Dawolo, Januari; Zebua, Okniel
Jurnal Ilmiah PLATAX Vol. 14 No. 1 (2026): ISSUE JANUARY-JUNE 2026
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v14i1.65990

Abstract

Seagrass ecosystems are essential components of coastal environments and play a strategic role in maintaining the stability and sustainability of marine ecosystems. This study aimed to analyze seagrass species diversity and the role of seagrass biodiversity in supporting marine ecosystem conservation in the Nias Islands, North Sumatra. The research was conducted at three locations: La’aya Village (North Nias Regency), Fodo Waters (Gunungsitoli City), and waters near Sirombu Port (West Nias Regency), using the quadrat transect method during the lowest low tide. Data analysis included density, frequency, cover, Important Value Index (IVI), and the Shannon–Wiener diversity index (H′). The results identified five seagrass species, namely Syringodium isoetifolium, Cymodocea serrulata, Cymodocea rotundata, Halodule uninervis, and Thalassia hemprichii. Syringodium isoetifolium was the dominant species with the highest IVI across all study sites. The seagrass diversity index values ranged from 1.55 to 1.57, indicating a moderate level of diversity and a relatively stable community structure. These findings suggest that seagrass ecosystems in the Nias Islands are still functioning effectively and play a crucial role in supporting coastal stability, marine biodiversity, and as a scientific basis for sustainable marine ecosystem management and conservation. Keywords: coastal ecosystem; marine conservation; Nias Islands; seagrass biodiversity Abstrak Ekosistem lamun merupakan salah satu komponen penting ekosistem pesisir yang memiliki peranan strategis dalam menunjang keberlanjutan dan konservasi ekosistem laut. Penelitian ini bertujuan untuk menganalisis tingkat keanekaragaman jenis lamun serta peranan biodiversitas lamun dalam mendukung upaya konservasi ekosistem laut di Kepulauan Nias, Sumatera Utara. Penelitian dilaksanakan pada tiga lokasi, yaitu Perairan Desa La’aya (Kabupaten Nias Utara), Perairan Fodo (Kota Gunungsitoli), dan Perairan dekat Pelabuhan Sirombu (Kabupaten Nias Barat). Metode yang digunakan adalah metode transek kuadrat pada saat surut terendah, dengan analisis struktur komunitas meliputi kerapatan, frekuensi, penutupan, Indeks Nilai Penting (INP), serta indeks keanekaragaman Shannon–Wiener (H′). Hasil penelitian menunjukkan bahwa terdapat lima jenis lamun, yaitu Syringodium isoetifolium, Cymodocea serrulata, Cymodocea rotundata, Halodule uninervis, dan Thalassia hemprichii. Syringodium isoetifolium merupakan spesies dominan di seluruh lokasi dengan nilai INP tertinggi. Nilai indeks keanekaragaman lamun pada ketiga lokasi berada pada kategori sedang, dengan nilai H′ berkisar antara 1,55–1,57. Kondisi ini menunjukkan bahwa struktur komunitas lamun relatif stabil dan masih mampu menjalankan fungsi ekologisnya secara optimal. Keanekaragaman lamun yang masih terjaga berperan penting dalam mendukung stabilitas ekosistem pesisir, penyediaan habitat biota laut, serta menjadi dasar ilmiah dalam perencanaan pengelolaan dan konservasi ekosistem laut di Kepulauan Nias secara berkelanjutan. Kata kunci: Kata kunci: biodiversitas lamun; ekosistem pesisir; konservasi laut; Kepulauan Nias
Co-Authors Alfret Luasunaung Betzy Victor Telaumbanua Deiske Adeliene Sumilat, Deiske Adeliene Destriman Laoli Dewi Zebua, Ratna Halawa, Vince Kurniawan Inneke F. M Rumengan Ixchel F Mandagi Januari Dawolo Kristoper Angandowa Waruwu, Vance Makapedua, Daisy M. Masengi, Akira W. R. Masengi, E. I. K.G. Masengi, K.W.A Okniel Zebua Sanora Laia, Dian Agung Telaumbanua, Afred Nobel Telaumbanua, Betzy V. Victoria E. N. Manoppo Wulur, Stenly Zebua, Arismanto Zebua, Okniel Zebua, Ratna Dewi