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All Journal AQUATIC SCIENCE & MANAGEMENT JURNAL ILMIAH PLATAX
Makapedua, Daisy M.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Management of scad fisheries (Decapterus spp.) in Sulawesi Sea Waters, North Sulawesi Province, using EAFM Puansalaing, Deysy M.; Budiman, Johnny; Boneka, Farnis B.; Makapedua, Daisy M.; Lasut, Markus T.; Ngangi, Edwin L.A.; Sumilat, Deiske A.; Darmono, Oktaviano P.
AQUATIC SCIENCE & MANAGEMENT Vol 9, No 1 (2021): APRIL
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.9.1.2021.32468

Abstract

There are a large variety and quite abundant types of small pelagic fish that have high economic value in the Sulawesi Sea. One of which is the blue scad fish or commonly known as malalugis (Decapterus macarellus). This study aims to analyze and determine the status of scad fisheries management and to develop recommendations in the management of scad fisheries in the waters of Sulawesi Sea, North Sulawesi Province. The evaluation of fishery management status is carried out using multi-criteria analysis (MCA) through the development of composite index of each indicator of Ecosystem Approach to Fisheries Management (EAFM). The results showed that, in general, the status of scad fisheries management in North Sulawesi Province, was in ‘good’ category, specifically reviewed from the domain of fish resources fall into the category of “medium”, habitat and ecosystem “good”, fishing techniques “good”, economy “not good”, social “medium” and institutional “good”. Management actions take precedence over domains that have “poor” indicator values. Priority management action is implemented in the economic domain, followed by the domain of fish resources, social, institutional and fishing techniques.Indonesian title: Pengelolaan perikanan ikan layang (Decapterus spp.) di perairan Laut Sulawesi, Provinsi Sulawesi Utara, menggunakan EAFM
Institutional Domain Assessment of the EAFM Approach to Snapper and Grouper Fisheries in the waters of the Sangihe Islands, North Sulawesi dumas, Davis Wijaksana Extrada; Lasabuda, Ridwan; Manembu, Indri Shelovita; Makapedua, Daisy M.; Darwasito, Suria; Luasunaung, Alfrets; Sumilat, Deiske Adeliene; Darmono, Oktaviano P.
Jurnal Ilmiah Platax Vol. 12 No. 1 (2024): ISSUE JANUARY-JUNE 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i1.52237

Abstract

This research aims to assess the status of snapper and grouper fisheries management in the Sangihe Islands district using an ecosystem approach (EAFM) in the institutional domain. The research method uses observation and interview methods (questionnaires). Data collection used semi-structured interview (SSI) techniques. As a result of the assessment of 6 (six) institutional indicators, there are 2 indicators with a value of 1 (less, red flag model), namely indicators of compliance with the principles of responsible fisheries and indicators of fisheries management plans. 3 indicators each: decision-making mechanism indicators, indicators of the level of synergy of fisheries management policies & institutions, and stakeholder capacity indicators) with a value of 2 (medium, yellow model flag). Only the indicator for the completeness of the rules in fisheries management has a value of 3 (good, green model flag). The average score for the 6 indicators is 1.76 while the composite value is 58.53. This value shows that the application of the EAFM institutional domain in the management of snapper and grouper fisheries in the Sangihe Islands district is in the medium category (yellow model flag). Keywords: EAFM, Sangihe Islands, Grouper Snapper Fishery, Flag Model. Abstrak Penelitian ini bertujuan untuk menilai  status pengelolaan perikanan kakap dan kerapu di kabupaten Kepulauan Sangihe dengan pendekatan ekosistem (EAFM) pada domain kelembagaan. Metode penelitian menggunakan metode observasi dan wawancara (kuisioner). Pengumpulan data menggunakan teknik semi structured interview (SSI).  Hasil penilaian dari 6 (enam) indikator kelembagaan, ada 2 indikator yang nilai-nya 1 (kurang, flag model merah) yaitu  indikator kepatuhan terhadap prinsip-prinsip perikanan yang bertanggung jawab dan indikator rencana pengelolaan  perikanan. 3 indikator masing-masing : indikator mekanisme pengambilan  keputusan, indikator tingkat sinergisitas kebijakan & kelembagaan pengelolaan perikanan, indikator kapasitas pemangku  kepentingan) bernilai 2 (sedang, flag model kuning). Hanya indikator kelengkapan aturan main dalam  pengelolaan perikanan yang bernilai 3 (baik, flag model hijau).  Nilai skor rerata 6 indikator adalah 1,76 sedangkan  nilai komposit-nya 58,53. Nilai ini menunjukkan bahwa penerapan domain kelembagaan EAFM pada pengelolaan perikanan kakap dan kerapu di kabupaten Kepulauan Sangihe kategori sedang (flag model kuning). Kata kunci : EAFM, Kepulauan Sangihe, Perikanan Kerapu Kakap, Model Bendera
Study of e-DNA Quality at Fishing Ground of Manado Bay, North Sulawesi Province. Zebua, Nistiarni; Mandagi, Ixchel F.; Masengi, K.W.A; Luasunaung, Alfret; Rumengan, Inneke F. M.; Wulur, Stenly; Makapedua, Daisy M.; Masengi, E. I. K.G.; Sumilat, Deiske A.; Masengi, Akira W. R.; Manoppo, Victoria
Jurnal Ilmiah Platax Vol. 13 No. 1 (2025): ISSUE JANUARY-JUNE 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i1.57404

Abstract

We used the Nansen Bottle Sampler to collect water samples in the deepsea area, ranging from 150 meters to 175 meters in six water points around Manado Bay, to test the quality of e-DNA water samples to detect target species in the fishing area. Therefore, the basis of the case study method with a sampling technique was carried out on July 29 2023 using Power Water Sterivex Kits, water samples were then stored at -250C and were then taken to TBRC, University of the Ryukyus for further laboratory works, such as; eDNA extract, eDNA quality testing, 1st and 2nd PCR and Electrophoresis eDNA analysis processes using MiFish-U primers with a target of 163–185bp and 375 bp, following the MiFish protocol.  Based on the results of the eDNA extract solution, it is known that the quality of eDNA from the 6 sampling sites locations ranged between 2.8 µg/mL – 4.4 µ g/mL, which means a good quality of eDNA. Moreover, it showed that the presence of DNA fragments at Kappa 60◦C Gelelectrophoresis 1st-PCR, 12S rRNA gene product (163–185bp), and Kappa 60◦C and 65◦C Geklelectrophoresis 2nd-PCR Products according to the target amplicon 375 bp. This means we can conduct the next step, the PCR sequence analysis.  Then, eDNA quality testing, 1st and 2nd PCR, and Electrophoresis of e-DNA analysis process were using MiFish-U F/R primers with a target of 375 bp, it is known that the concentration of Nanodrop from the 6 sampling locations ranges between 2.8 µg/mL – 4.4 µg/mL while the core or quality eDNA ranged from 1.56 µg/mL – 2.50 µg/mL. Based on identification results, five types of species were detected; Myctophum lychnobium, Selar crumenophthalmus, Photonectes sp., Oreochromis sp. Thunnus obesus and Homo sapiens were generated using eDNA metabarcoding on the mitochondria genome database MitoFish. Keywords: e-DNA, eDNA quality, species target, fishing area, Manado Bay   Abstrak Kami menggunakan Nansen Bottle Sampler untuk mengambil sampel air pada laut dalam berkisar 150meter sampai 175 meter di enam titik perairan Sekitar Teluk Manado, untuk menguji Kualitas e-DNA sample air yang digunakan untuk mendeteksi target spesies pada daerah penangkapan. Selanjutnya dasar metode studi kasus dengan teknik pengambilan sampel secara sampling dilakukan pada tanggal 29 Juli 2023 menggunakan Power Water Sterivex Kits, sapel air disimpan pada -250C yang selanjutnya dibawa ke TBRC, University of the Ryukyus untuk pnelitian laboratorium lanjutan seperti ekstrak eDNA, Pengujian kualitas eDNA, 1st and 2nd PCR dan Elektrophoresis proses analisis eDNA menggunakan primer MiFish-U dengan target 375 bp, mengikuti MiFish protokol. Berdasarkan hasil pengujian larutan ekstrak eDNA diketahui bahwa kualitas eDNA dari 6 titik lokasi sampling berkisar antara 2.8 ng/mL – 4.4 ng/mL dan menunjukkan adanya fragment DNA pada Kappa 60◦C Geklelectrophoresis 1st-PCR Produk 12S rRNA gene (163–185bp, dan Kappa 60◦C dan 65◦C Geklelectrophoresis 2nd-PCR Produk sesuai amplikon target 375 bp. Hal ini berarti dapat dilanjutkan pada tahap analisis sekuens PCR. Pengujian kualitas eDNA, 1st and 2nd PCR dan Elektrophoresis proses analisis eDNA menggunakan primer MiFish-U F/R dengan target 375 bp, diketahui bahwa konsentrasi Nanodrop dari 6 titik lokasi sampling berkisar antara 2.8 µg/mL – 4.4 µg/mL sedangkan kemurnian atau kualitas eDNA berkisar antara 1.56 µg/mL – 2.50 µg/mL. Hasil identifikasi menyatakan lima jenis spesies terdeteksi; Myctophum lychnobium, Selar crumenophthalmus, Photonectes sp., Oreochromis sp. Thunnus obesus, Homo sapiens dihasilkan dengan menggunakan eDNA metabarcoding pada MitoFish database genom mitokondria. Kata kunci : e- DNA, kualitas eDNA, target spesies, daerah penangkapan, Teluk Manado.
Co-Authors Alfret Luasunaung Darmono, Oktaviano P. Darwasito, Suria Deiske Adeliene Sumilat, Deiske Adeliene Deysy M. Puansalaing dumas, Davis Wijaksana Extrada Edwin D Ngangi Farnis B. Boneka Indri Manembu Inneke F. M Rumengan Ixchel F Mandagi Johnny Budiman Luasunaung, Alfrets Manoppo, Victoria Markus T. Lasut Masengi, Akira W. R. Masengi, E. I. K.G. Masengi, K.W.A Ridwan Lasabuda Wulur, Stenly Zebua, Nistiarni