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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya JIMR - Journal of Islamic Medicine Research Jurnal Kesehatan Islam : Islamic Health Journal Jurnal Kedokteran Komunitas (Journal of Community Medicine) Jurnal Kedokteran Hewan Journal of Tropical Pharmacy and Chemistry
Doti Wahyuningsih
Fakultas Kedokteran, Universitas Islam Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Promegranate Peel as Prophylactic Agent of Rheumatoid Arthritis : Study on MMP-9, IL-6, TNF- α, and Arthritic Score of Rheumatoid-arthritic Rats Wahyuningsih, Doti; Amilia, Annisa; Amiruddin, Meilano Setiawan; Cahyaningrum, Asri; Kurniasari, Laili Cinthianur
Journal of Tropical Life Science Vol 10, No 1 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.218 KB) | DOI: 10.11594/jtls.10.01.08

Abstract

Rheumatoid arthritis (RA) is a joint disease, chronically cause permanent joint impairment leading to inability of daily life activities. Early diagnosis and preven-tive managements of RA are recommended to overcome the disease. The current drugs provide benefits for RA patients, unfortunately could not be used for long period and as prevention agents, due to the adverse effects.  Even though clinical and laboratory studies of pomegranate for osteoarthritis and RA had been done, the effects of the pomegranate peel on MMP-9, TNF-α, and IL-6 of RA are still unrevealed. TNF-α promotes inflammation process in RA and collaborates with osteoclasts to trigger osteoporosis. IL-6 shows negative effect on osteoblasts dif-ferentiation and MMP-9 stimulates cartilage degradation and inflammation mediat-ed by synovial fibroblast. This study measured TNF-α, IL-6, MMP-9, and arthri-tis score (AS) of RA rats treated with ethanolic extract of pomegranate peel (EPP) to evaluate its potency as a RA prevention agent. Male Lewis rats (three groups, five each), 200 g, received 80 mg, 160 mg, and 320 mg of EPP/rat respectively, in alternate day, within 60 days. On the 30th day, the rats were subcutaneously in-jected with 0,1 ml mycobacterium-complete Freund’s adjuvant (1 mg/mL) on plan-tar of the right hind paws to induce RA. Serum IL-6 and TNF-α were determined by ELISA.  Immunohistochemistry processed-synovial MMP-9 slices of ankle joints were evaluated by light microscope (400× magnification).  Arthritis score of Smit was used to determined AS. Data were analyzed by Kruskall Wallis, Mann Whitney U, and Pearson correlation test. p < 0.05 was significant. The EPP of 320 mg corrected serum TNF-α and IL-6, and synovial MMP-9 of RA rats (p < 0.05). No significant change was observed in arthritic score following the EPP treatment (p > 0.05). In conclusion, the results indicate the EPP may potential to be devel-oped as preventive agent of rheumatoid arthritis.
Red Yeast Rice Protects Hepatocytes conditions of Rats Receiving High Fat Diet Wahyuningsih, Doti; Elyani, Helmin; Damayanti, Dini Sri; Yahya, Arif; Fadli, Muhammad Zainal
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.954 KB) | DOI: 10.11594/jtls.09.02.06

Abstract

Triglyceride (TG) is a simple and the main lipid of the daily diet. Nowadays, TG is emerging as an independent risk factor for cardiovascular disease, and increasing clinical data that indicate a high level of this simple lipid in serum may alert and play a role in liver impairment. Red yeast rice (RYR) reduces serum TG in human and animals. The rice is widely used as a natural inhibitor of HMG-CoA reductase to correct serum cholesterol level. Bioactive components of the rice are well known to have antioxidant properties. This study examined whether RYR protects hepatocytes by evaluating the serum AST-ALT, HDL-Cholesterol (HDL-C), TG levels, and the number of liver foam cells in hypertriglyceridemic rats. Twenty male Wistar rats were grouped into 5. Four groups received a high-fat diet (HFD), 40 g/animal/day for 60 days to induce hypertriglyceridemic condition. Along with the HFD treatment, three groups received 108, 54, and 27 mg/kgBW/day of RYR, respectively. Two other groups received standard and only HFD diet, respectively. Intracardiac blood was collected for measuring AST and ALT using AST or ALT activity assay Kit, respectively, serum TG and HDL-C by Enzymatic Caloric Test.  Hematoxylin-Eosin–stained 4µmm thick slices of liver tissues were prepared to count foam cell number by a light microscope with 400x magnificence. Data were analyzed using the Kruskal Wallis continued by the Mann Whitney U test. The p values of < 0.05 were considered to be significant. The present study found that 108, 54, and 27 mg/kgBW/day of RYR significantly decreased serum TG, HDL-C, AST, and ALT compared with those of hypertriglyceridemic rats receiving no RYR. The decreasing levels of the parameters were in relation to the doses of RYR. The doses of 108 and 54 mg/kgBW/day resulted in complete recovery of the liver tissues suffered from steatosis (p < 0.05). The RYR dose of 108 mg/KgBW/day completely corrects the serum HDL-C level. In conclusion, red yeast rice may have a potency to protect hepatocytes injuries due to hypertriglyceridemia.
Co-Authors Adam Bregas Regowo Alfania Novita Putri Perdana Amilia, Annisa Amiruddin, Meilano Setiawan Andri Tilaqza Andrie Devin Susilo Hadi Anita Puspa Widiyana Ariya Indra Krisna Cahyaningrum, Asri Damayanti, Dini Sri Dewi Intan Putri Dwiyanti Elyani, Helmin Fadli, Muhammad Zainal Faizah Dwi Qurrotul Aini Fidurroty Baidho Fifin Pradina Duhitatrissari Hanny Hafiar Helmin Elyani Ike Widyaningrum Kurniasari, Laili Cinthianur Marindra Firmansyah Merlita Herbani Muhammad Yusuf Musthafa Nour Athiroh Abdoes Sjakoer Nugroho Wibisono Siti Norazizah Vina Zahirotul Izzah Yahya, Arif Yoyon Arif Martino Yudi Purnomo Yundari, Yundari