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All Journal FaST : Jurnal Sains dan Teknologi BIODIVERS - BIOTROP Scientific Magazine
Suryadi, Jennifer Claudia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Energy Environmental Science

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The Effect of Mycorrhiza Application and Phosphorus Addition on AMF Spores Density and Pueraria javanica Growth Rosita, Risa; Suryadi, Jennifer Claudia; Eris, Deden Dewantara
BIODIVERS - BIOTROP Science Magazine Vol. 4 No. 1 (2025): Vol. 4 No. 1 (2025): BIODIVERS Volume 4 No. 1 (2025)
Publisher : SEAMEO BIOTROP

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Abstract

One of the suitable host plants for AMF (Arbuscular Mycorrhizal Fungi) is Pueraria javanica. Several factors that can affect the growth of P. javanica are the availability of nutrients and the activity of potential soil microbes, such as AMF. Applying potential soil microbes (AMF) to plants can increase plant growth, chlorophyll levels, and enzyme activity, and even improve soil quality. The ability of AMF to increase plant nutrient absorption causes plants with AMF tend to have optimum growth. The study aimed to understand the effect of AMF application and phosphorus (P) nutrients addition on AMF spore density and P. javanica growth. The experiment used a completely randomized design with one factor (formulation). Results of the study proved that phosphorus (P) addition was able to reduce AMF spore densities (Glomus etunicatum and Glomus mosseae) 3 weeks after application (WAA). The availability of P in a fairly high amount around the root area caused plants to reduce their dependence on AMF, which resulted in a decrease in AMF colonization and AMF spore densities. On the other hand, adding P nutrients proved to increase growth parameters (plant height and number of leaves) of P. javanica because AMF helped the absorption of P and received carbon from plants in return. Treatment P6 (G. etunicatum 10 g + without phosphorus) had the highest spore density value (400.33/10 g planting media), and treatment P9 (G. mosseae 10 g + phosphorus 10 ppm) showed the highest increase in plant height of 13.333 (P < 0.05) 3 weeks after application. Meanwhile, the maximum increase in the number of leaves occurred in plants that received 10 g of G. mosseae and 10 ppm of P every two days for three weeks. Studying AMF spore density can significantly improve plant growth, agronomic efficiency, and agricultural sustainability.
Pemanfaatan Kolagen Limbah Ikan Tuna dalam Meningkatkan Pertumbuhan Bacillus Subtilis sebagai Kandidat Probiotik: [Utilization of Collagen From Tuna Fish Waste to Enhance The Growth of Bacillus Subtilis as A Probiotic Candidate] Christy, Suawa Natania Abigail; Wijaya, Accoladea; Putri, Angela Clarita; Suryadi, Jennifer Claudia; Yap, Cecilia Aileen; Simamora, Chelsea Valeria; Tjhajadi, Jennifer Patricia; Thanos, Celeste Abigail; Theodora, Gisella; Irawan, Michella; Soputro, Veronica Tammy; Thenoch, Adrian Samuel; Pinontoan, Reinhard; Jo, Juandy
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 8 No. 2 (2025): MAY
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jstfast.v9i1.9730

Abstract

Probiotics are a group of bacteria with health benefits, which are widely used in various nutritional products. Various efforts are made to effectively increase growth of probiotics, including adding supplemental nutrition, such as protein. In this study, a series of tests were conducted (optical density, colony forming unit, and pH test) to evaluate the effect of collagen hydrolysate supplementation, obtained from tuna waste, on the growth of Bacillus subtilis G8, a candidate of probiotic bacteria which have been isolated from the Japanese traditional food natto. The results demonstrated positive impacts of collagen hydrolysate supplementation on cell density and colony-forming units, as well as on the reduction of pH of bacterial growth medium. Concentration of 5% was observed as the most optimum concentration to support the bacterial growth, while the most acidic pH of bacterial growth medium is observed at 3% supplementation. In conclusion, supplementation of collagen hydrolysate derived from tuna waste supported the growth of B. subtilis G8 as well as reduced the pH of its growth media. Bahasa Indonesia Abstract: Probiotik merupakan sekelompok bakteri yang memiliki manfaat kesehatan dan banyak digunakan dalam berbagai produk nutrisi. Beragam upaya telah dilakukan untuk secara efektif meningkatkan pertumbuhan probiotik, salah satunya melalui penambahan nutrisi tambahan seperti protein. Dalam penelitian ini, serangkaian uji dilakukan, meliputi uji kerapatan optik, unit pembentuk koloni (CFU), dan pengukuran pH, untuk mengevaluasi pengaruh suplementasi kolagen hidrolisat yang diperoleh dari limbah ikan tuna terhadap pertumbuhan Bacillus subtilis G8, yakni kandidat bakteri probiotik yang diisolasi dari makanan tradisional Jepang, natto. Hasil penelitian menunjukkan bahwa suplementasi kolagen hidrolisat memberikan dampak positif terhadap kerapatan sel dan jumlah koloni, serta menyebabkan penurunan pH medium pertumbuhan bakteri. Konsentrasi 5% diketahui sebagai konsentrasi yang paling optimal dalam mendukung pertumbuhan bakteri, sedangkan pH terendah pada medium pertumbuhan tercatat pada konsentrasi suplementasi sebesar 3%. Dengan demikian, dapat disimpulkan bahwa suplementasi kolagen hidrolisat yang berasal dari limbah ikan tuna mampu mendukung pertumbuhan B. subtilis G8 serta menurunkan pH media pertumbuhannya.  
Co-Authors Christy, Suawa Natania Abigail Eris, Deden Dewantara Irawan, Michella Juandy Jo Putri, Angela Clarita Reinhard Pinontoan ROSITA, Risa Simamora, Chelsea Valeria Soputro, Veronica Tammy Thanos, Celeste Abigail Thenoch, Adrian Samuel Theodora, Gisella Tjhajadi, Jennifer Patricia Wijaya, Accoladea Yap, Cecilia Aileen