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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Rekayasa Bahan Alam dan Energi Berkelanjutan Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) BIODIVERS - BIOTROP Scientific Magazine
Eris, Deden Dewantara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Veterinary

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Synthesis of Chitosan Composite based on Black Soldier Fly (BSF) Exuviae with Kipahit Leaf Extract and its Inhibition Test against Xanthomonas Oryzae. Wahyuni, Sri; Fauziyah, Ridha; Aziz, Muhammad Abdul; Eris, Deden Dewantara; Prakoso, Haryo Tejo; siswanto, Siswanto; Priyono, Priyono
Jurnal Rekayasa Bahan Alam dan Energi Berkelanjutan Vol. 5 No. 2 (2021)
Publisher : Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rbaet.2021.005.02.03

Abstract

HDB disease caused by the bacteria Xanthomonas oryzae pv.oryzae (Xoo) is an important disease in rice plants. Recently, many organic based antibacterial agents are being developed, one of which is chitosan. One of the potential raw materials for producing chitosan is Black Soldier Fly (BSF) exuviae. During the development, chitosan was formulated with other ingredients such as kipahit leaves (Tithonia diversifolia). The purpose of this study is to synthesize BSF-based chitosan, formulate BSF-based chitosan composites with kipahit leaf extract using 0.1% sodium tripolyphosphate and test the antibacterial activity against Xanthomonas oryzae composites. The process of optimizing the extraction of kipahit leaves was carried out with a variety of solvents (2% acetic acid, 96% ethanol and 20% DMSO). The antibacterial activity test was carried out by the disc diffusion method at a concentration of 0.1; 0.2; 0.4; 0.6; 0.8 and 1% (w / v). The results showed that the best kipahit leaf extraction was obtained using 96% ethanol as a solvent. 
The Effect of Mycorrhiza Application and Phosphorus Addition on AMF Spores Density and Pueraria javanica Growth Rosita, Risa; Suryadi, Jennifer Claudia; Eris, Deden Dewantara
BIODIVERS - BIOTROP Science Magazine Vol. 4 No. 1 (2025): Vol. 4 No. 1 (2025): BIODIVERS Volume 4 No. 1 (2025)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One of the suitable host plants for AMF (Arbuscular Mycorrhizal Fungi) is Pueraria javanica. Several factors that can affect the growth of P. javanica are the availability of nutrients and the activity of potential soil microbes, such as AMF. Applying potential soil microbes (AMF) to plants can increase plant growth, chlorophyll levels, and enzyme activity, and even improve soil quality. The ability of AMF to increase plant nutrient absorption causes plants with AMF tend to have optimum growth. The study aimed to understand the effect of AMF application and phosphorus (P) nutrients addition on AMF spore density and P. javanica growth. The experiment used a completely randomized design with one factor (formulation). Results of the study proved that phosphorus (P) addition was able to reduce AMF spore densities (Glomus etunicatum and Glomus mosseae) 3 weeks after application (WAA). The availability of P in a fairly high amount around the root area caused plants to reduce their dependence on AMF, which resulted in a decrease in AMF colonization and AMF spore densities. On the other hand, adding P nutrients proved to increase growth parameters (plant height and number of leaves) of P. javanica because AMF helped the absorption of P and received carbon from plants in return. Treatment P6 (G. etunicatum 10 g + without phosphorus) had the highest spore density value (400.33/10 g planting media), and treatment P9 (G. mosseae 10 g + phosphorus 10 ppm) showed the highest increase in plant height of 13.333 (P < 0.05) 3 weeks after application. Meanwhile, the maximum increase in the number of leaves occurred in plants that received 10 g of G. mosseae and 10 ppm of P every two days for three weeks. Studying AMF spore density can significantly improve plant growth, agronomic efficiency, and agricultural sustainability.
Characterization of Phosphate Solubilizing Bacteria from Three Types of Soil Rhizosphere and Their Potency to Increase Growth of Corn Plants (Zea mays) Rosita, Risa; Apriana, Edwin; Hazra, Fahrizal; Eris, Deden Dewantara
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.288

Abstract

Pemberian dosis pupuk P (fosfat) yang tinggi tidak sejalan dengan ketersediaan P dalam tanah karena sebagian besar P terikat oleh Al, Fe dan Ca sehingga tidak tersedia bagi tanaman. Pemanfaatan mikrob pelarut P merupakan salah satu upaya dalam meningkatkan ketersediaan P yang dapat diserap oleh tanaman. Tujuan dari penelitian ini adalah untuk mengisolasi, mengkarakterisasi dan menguji potensi mikroba pelarut P yang diisolasi dari tiga jenis rizosfer. Penelitian menggunakan Rancangan Acak Lengkap (RAL) satu faktorial. Serangkaian karakterisasi melalui uji hipersensitivitas, uji pewarnaan gram, uji katalase, uji oksidase, uji kebutuhan oksigen, uji motilitas, kemampuan menggunakan berbagai sumber karbon (glukosa, laktosa, dan sukrosa), uji metil merah, dan uji pertumbuhan pada berbagai pH . Hasil penelitian menunjukkan sebanyak 5 bakteri pelarut P terpilih (Ca-Al-7, Ca-Al-8, Ca-Al-4, Ca-NF-1, dan Ca-NF-3) bukan patogen tanaman, dengan nilai kelarutan fosfat berturut-turut 1,7; 2,9; 2,5; 3,1; 3,2 berpengaruh nyata terhadap parameter tinggi tanaman, jumlah daun, biomassa segar dan kering tanaman Zea mays setelah inokulasi 4 minggu (p < 0,05).
EFFECTIVENESS OF LIQCORIS ORGANIC PESTICIDE AS GROWTH INHIBITOR OF PLANT PATHOGENIC MICROORGANISMS Rosita, Risa; Eris, Deden Dewantara; Bait, Misbakhul; Imran, Dr. Zulhamsyah; Widayanti, Sri; Bigcas, Evelyn V.; Subchan, Wachju
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2347

Abstract

Article Highlights:- First study on Liqcoris, an organic pesticide made from coconut waste.- 15% Liqcoris concentration effectively inhibits pests and plant pathogens.- Active compounds include phenol and carbamic acid.- Pest and fungal pathogens were isolated directly from diseased plants in a school garden.- Coconut waste-based pesticide offers practical, eco-friendly pest control solutions. Abstract:Sustainable control of plant diseases is essential to maintain biodiversity. Learning using prototypes is an effort to support students in understanding and learning science and technology in order to answer problems related to biodiversity loss. The research aimed to: 1) produce a prototype of Liqcoris organic pesticide (BLM 06) from coconut shell and shell waste, 2) measure the ability of the Liqcoris organic pesticide in inhibiting the growth of plant pathogenic microorganisms, and 3) study the content of  Liqcoris organic pesticide. The data obtained were statistically analyzed using the SAS Version 9.0 program. The analysis carried out was the analysis of variance (ANOVA). Duncan's advanced test was used at the 5% level to determine the differences among the treatments, i.e., 00 = control or without PDA + pesticide; 10 = PDA +  pesticide concentration 10%; 15 = PDA + pesticide concentration 15%, 20 = PDA + pesticide concentration 20%, and 25 = PDA + pesticide concentration 25%. Based on in vitro test, the study showed that Liqcoris concentration of 15% proved effective in killing phytopathogen fungi (P < 0.05), including Fusarium equiseti, Fusarium graminearum, Nigrospora sphaerica and Colletotrichum gloeosporioides. Treatments with various concentrations of 15%, 20%, and 25% effectively produced inhibitory values ​​in the diameter of pathogenic fungal colonies, respectively 0.118 cm, 0.000 cm, and 0.000 cm that were significantly different from concentrations of 10% (2.7140 cm) and control 0% (5.2180 cm). Considering the economic value of production, the best concentration of organic pesticides chosen was 15%. On the other hand, in vivo test results indicated that in controlling mosaic viruses on diseased curly chili variety TM 999, without Liqcoris treatment (control), the intensity of disease attack increased by 20.22% after 6 weeks after treatment (wat). Meanwhile, after applying Liqcoris organic pesticide with a concentration of 15%, the percentage of mosaic virus attacks decreased to 2.72% after 6 wat (P < 0.05). This study showed that the content of 15% Liqcoris organic pesticide was dominated by active compound phenol (35.16%) and carbamic acid, phenyl ester (23.61%).
Co-Authors Apriana, Edwin Bait, Misbakhul Bigcas, Evelyn V. Fahrizal Hazra Fauziyah, Ridha Imran, Dr. Zulhamsyah PRAKOSO, Haryo Tejo Priyono Priyono ROSITA, Risa RR. Ella Evrita Hestiandari S Siswanto Sri Wahyuni Sri Widayanti Suryadi, Jennifer Claudia Wachju Subchan