Background: According to WHO in 2020 in Hayati (2021) around 20% experience anxiety, this condition is higher in developing countries, reaching 15.6% during pregnancy and 19.8% after childbirth. In Indonesia, according to the Indonesian Ministry of Health in Suhada 2019, there are around 28.7% of pregnant women who experience anxiety in the third trimester. Based on research conducted by Lisa and Siti (2021) in Depok, that prenatal yoga is very effective in reducing anxiety in pregnant women.The researcher tried to conduct a brief interview with 10 pregnant women, out of 10 pregnant women only 1 person (10%) had done yoga exercises. This study aims to identify the frequency distribution of respondents with the interest of pregnant women in doing prenatal yoga based on age, education, information sources, knowledge of pregnant women at TPMB N Bojongsari depok in 2023, and analyze the relationship between knowledge and the interest of pregnant women in doing prenatal yoga at TPMB.N Bojongsari Depok in 2023.Methods: This study used the analytical correlation method with a cross-sectional design. The population in this study were all pregnant women in the second and third trimesters at TPMB N. Bojongsari Depok, with a total of 30 people. The sample was total sampling.Results: From 30 samples obtained most of them, namely 16 respondents (53.3%) have good knowledge about prenatal yoga and those who have high interest in doing prenatal yoga are 15 respondents (50%). And still 14 respondents (46.7%) have less knowledge about prenatal yoga with low interest in doing prenatal yoga 8 respondents (26.7%). Chi square test shows p value 0.004 with OR = 12.Conclusion: There is a relationship between knowledge and the interest of pregnant women in doing prenatal yoga. In increasing the interest of pregnant women in doing prenatal yoga, good knowledge about prenatal yoga is needed.