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All Journal Jurnal Pembelajaran dan Biologi Nukleus Journal of Pharmaceutical and Sciences.
Siboro, Dewi Pratiwi Purba
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Development of Species-Specific Primers Targeting Mitochondrial Cyt b Gene for Porcine DNA Detection in Halal Authentication via Polymerase Chain Reaction (PCR) Malau, Jekmal; Kasasiah, Ahsanal; Zahra, Aliya Azkia; Irwansyah, Silvana Lestari; Siboro, Dewi Pratiwi Purba
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.6799

Abstract

Background: The detection of porcine DNA is critical to ensuring adherence to halal standards, particularly in food and pharmaceutical products. This study aimed to design and validate species-specific primers targeting the mitochondrial Cyt b gene of Sus scrofa for porcine DNA identification. Using in silico tools such as NCBI, Primer3Plus, SnapGene, Mega and NetPrimer, four primer pairs were designed and assessed for specificity and efficiency. Methodology: Laboratory validation involved PCR amplification and bi-directional Sanger sequencing. Findings: The findings demonstrated that primers 2F/2R and 3F/3R successfully amplified the target DNA, producing amplicons of 514 bp and 456 bp, respectively. The primers exhibited high specificity, with no amplification observed in non-target DNA samples, including chicken and beef. Sanger sequencing confirmed that the amplified products corresponded to the Cyt b gene sequence of Sus scrofa with 100 % similarity, as validated through BLAST analysis. This study presents an accurate and dependable molecular method for detecting porcine DNA, with valuable applications in halal authentication and molecular diagnostics. Contribution: The developed primers offer an effective tool for accurately identifying porcine-derived components, addressing the critical demand for species-specific DNA detection to support halal compliance.
Menelusuri Perkembangan, Kondisi Terkini, dan Prospek Masa Depan Pengujian DNA dalam Aplikasi Forensik Manusia dan Non-Manusia: Tinjauan Naratif Siboro, Dewi Pratiwi Purba; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Malau, Jekmal; Aprillia, Cantika; Ainaputri, Aliza Salsabila; Nugraha, Afif Tri; Aprianti, Endeh
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1389

Abstract

DNA typing has become a cornerstone of modern forensic science, profoundly influencing criminal investigations, forensic human identification, and non-human forensic applications. Since its introduction in the mid-1980s, forensic DNA analysis has evolved from restriction fragment length polymorphism-based methods to polymerase chain reaction-based short tandem repeat profiling, and more recently to sequence-based approaches enabled by massively parallel sequencing, resulting in substantial improvements in analytical sensitivity, robustness, and discriminatory power. This narrative review aims to trace the historical development, examine the current state, and explore future directions of DNA typing in both human and non-human forensic contexts, with particular emphasis on empirical case studies from Asia. A narrative review methodology was employed through a comprehensive analysis of peer-reviewed literature published between 2015 and 2026, sourced from major scientific databases including Google Scholar, PubMed, and ScienceDirect, with studies selected based on forensic relevance, methodological rigor, and regional significance. The review highlights the extensive application of DNA typing in routine criminal casework, disaster victim identification, missing persons investigations, wildlife forensic genetics, food fraud detection, and biosecurity, and documents emerging technologies such as portable DNA systems and CRISPR-based detection. Despite these advances, significant challenges remain related to data interpretation, validation requirements, contamination control, ethical and legal governance, and uneven forensic capacity across regions. Overall, this review underscores the continuing evolution of forensic DNA typing and emphasizes the importance of standardized protocols, interdisciplinary collaboration, and region-specific validation to ensure the reliable and responsible application of DNA evidence in modern forensic science.
Co-Authors Ahsanal Kasasiah Ainaputri, Aliza Salsabila Aprianti, Endeh Aprillia, Cantika Irwansyah, Silvana Lestari Jekmal Malau Nugraha, Afif Tri Zahra, Aliya Azkia