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All Journal Jurnal EduHealth Jurnal Sains dan Kesehatan
Irwansyah, Silvana Lestari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Primer Design of Porcine DNA using Mitochondrial DNA (Cyt b Gene) for Halal Authentication using Polymerase Chain Reaction (PCR) Rohmah, Siti; Irwansyah, Silvana Lestari; Kasasiah, Ahsanal; Malau, Jekmal
Jurnal Sains dan Kesehatan Vol. 7 No. 1 (2025): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v7i1.2418

Abstract

The Muslim community has difficulties in determining the halal status of animal-derived items and their derivatives due to their extensive prevalence. Islamic customers are increasingly becoming more selective and are demanding halal certification for culinary goods. Developing a way to identify porcine DNA in goods is of utmost importance. The Cyt b gene is being used to evaluate swine DNA samples by using primer candidates. The process of generating primers utilizing bioinformatics tools, such as NCBI, MegaXI, Primer3Plus, SnapGene Viewer, and Net Primer websites, is utilized to assess and analyze the effectiveness of laboratory research. The study concluded that the primers effectively amplified pig DNA, but they were unsuccessful in amplifying chicken or beef DNA. After conducting in silico experiments, a total of 7 possible primers were generated. The most advantageous pair of primers was identified, which includes the forward primer 5'-AACATCCGAAATCACACCC-3' and the reverse primer 5'-AGAATGATATTTGTCCTCAGGG-3'. The efficacy of these primers was evaluated in a controlled laboratory environment. Results from the laboratory experiments demonstrate that these particular primers have the ability to amplify the Cyt b gene from the Sus scrofa species at a temperature of 58°C, producing a DNA fragment that is 415 base pairs long. DNA sequencing is essential to verify that the amplified DNA band matches to the Sus scrofa Cyt b gene. Keywords:          PCR, Porcine, Primer, Cyt b, Halal
Influence Of Health Preventive Behavior On HIV/AIDS : Literature Review Irwansyah, Silvana Lestari; Hilmi, Indah Laily; Salman, Salman
Jurnal EduHealth Vol. 13 No. 02 (2022): Jurnal eduHealth, Periode Oktober - December, 2022
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.75 KB)

Abstract

HIV (Human Immunodeficiency Virus) is a major problem in many countries, including Indonesia. Preventive behavior towards HIV/AIDS must be accompanied by the implementation of appropriate health policy strategies in each country. Objective: to identify the behavior of HIV/AIDS prevention in Indonesian society. Method: literature study by choosing a topic, finding keywords, and making a journal review. Search through Google Scholar and scientific journals published nationally and internationally in the last 10 years, namely 2012 – 2022. Result: improvement in health services is needed such as HIV/AIDS counseling, peer education, VCT, and other innovative programs. Conclusion: it is necessary to strengthen community behavior to improve health status in order to prevent HIV from occurring in at-risk groups.
Co-Authors Ahsanal Kasasiah Aisiyah, Adella Indah Laily Hilmi Iskandar, Putri Mutiara Jekmal Malau Marsah Rahmawati Utami Mierza, Vriezka Nailuvar, Regita Nurfadhila, Lina Sakinah, Irene Virda Salman Salman salsabilla, Diva rizqi Siti Rohmah