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All Journal Jurnal Pembelajaran dan Biologi Nukleus Jurnal EduHealth Jurnal Sains dan Kesehatan
Irwansyah, Silvana Lestari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Veterinary

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Primer Design of Porcine DNA using Mitochondrial DNA (Cyt b Gene) for Halal Authentication using Polymerase Chain Reaction (PCR) Rohmah, Siti; Irwansyah, Silvana Lestari; Kasasiah, Ahsanal; Malau, Jekmal
Jurnal Sains dan Kesehatan Vol. 7 No. 1 (2025): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v7i1.2418

Abstract

The Muslim community has difficulties in determining the halal status of animal-derived items and their derivatives due to their extensive prevalence. Islamic customers are increasingly becoming more selective and are demanding halal certification for culinary goods. Developing a way to identify porcine DNA in goods is of utmost importance. The Cyt b gene is being used to evaluate swine DNA samples by using primer candidates. The process of generating primers utilizing bioinformatics tools, such as NCBI, MegaXI, Primer3Plus, SnapGene Viewer, and Net Primer websites, is utilized to assess and analyze the effectiveness of laboratory research. The study concluded that the primers effectively amplified pig DNA, but they were unsuccessful in amplifying chicken or beef DNA. After conducting in silico experiments, a total of 7 possible primers were generated. The most advantageous pair of primers was identified, which includes the forward primer 5'-AACATCCGAAATCACACCC-3' and the reverse primer 5'-AGAATGATATTTGTCCTCAGGG-3'. The efficacy of these primers was evaluated in a controlled laboratory environment. Results from the laboratory experiments demonstrate that these particular primers have the ability to amplify the Cyt b gene from the Sus scrofa species at a temperature of 58°C, producing a DNA fragment that is 415 base pairs long. DNA sequencing is essential to verify that the amplified DNA band matches to the Sus scrofa Cyt b gene. Keywords:          PCR, Porcine, Primer, Cyt b, Halal
Development of Species-Specific Primers Targeting Mitochondrial Cyt b Gene for Porcine DNA Detection in Halal Authentication via Polymerase Chain Reaction (PCR) Malau, Jekmal; Kasasiah, Ahsanal; Zahra, Aliya Azkia; Irwansyah, Silvana Lestari; Siboro, Dewi Pratiwi Purba
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.6799

Abstract

Background: The detection of porcine DNA is critical to ensuring adherence to halal standards, particularly in food and pharmaceutical products. This study aimed to design and validate species-specific primers targeting the mitochondrial Cyt b gene of Sus scrofa for porcine DNA identification. Using in silico tools such as NCBI, Primer3Plus, SnapGene, Mega and NetPrimer, four primer pairs were designed and assessed for specificity and efficiency. Methodology: Laboratory validation involved PCR amplification and bi-directional Sanger sequencing. Findings: The findings demonstrated that primers 2F/2R and 3F/3R successfully amplified the target DNA, producing amplicons of 514 bp and 456 bp, respectively. The primers exhibited high specificity, with no amplification observed in non-target DNA samples, including chicken and beef. Sanger sequencing confirmed that the amplified products corresponded to the Cyt b gene sequence of Sus scrofa with 100 % similarity, as validated through BLAST analysis. This study presents an accurate and dependable molecular method for detecting porcine DNA, with valuable applications in halal authentication and molecular diagnostics. Contribution: The developed primers offer an effective tool for accurately identifying porcine-derived components, addressing the critical demand for species-specific DNA detection to support halal compliance.
Influence Of Health Preventive Behavior On HIV/AIDS : Literature Review Irwansyah, Silvana Lestari; Hilmi, Indah Laily; Salman, Salman
Jurnal EduHealth Vol. 13 No. 02 (2022): Jurnal eduHealth, Periode Oktober - December, 2022
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.75 KB)

Abstract

HIV (Human Immunodeficiency Virus) is a major problem in many countries, including Indonesia. Preventive behavior towards HIV/AIDS must be accompanied by the implementation of appropriate health policy strategies in each country. Objective: to identify the behavior of HIV/AIDS prevention in Indonesian society. Method: literature study by choosing a topic, finding keywords, and making a journal review. Search through Google Scholar and scientific journals published nationally and internationally in the last 10 years, namely 2012 – 2022. Result: improvement in health services is needed such as HIV/AIDS counseling, peer education, VCT, and other innovative programs. Conclusion: it is necessary to strengthen community behavior to improve health status in order to prevent HIV from occurring in at-risk groups.
Co-Authors Ahsanal Kasasiah Aisiyah, Adella Indah Laily Hilmi Iskandar, Putri Mutiara Jekmal Malau Marsah Rahmawati Utami Mierza, Vriezka Nailuvar, Regita Nurfadhila, Lina Sakinah, Irene Virda Salman Salman salsabilla, Diva rizqi Siboro, Dewi Pratiwi Purba Siti Rohmah Zahra, Aliya Azkia